Restriction map for
pF1KSDA0924
(Circular) (Six-base) MAPSORT of: pF1KSDA0924.seq Check: 225 from: 1 to: 4955
pF1KSDA0924 4955 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1638 1781 1638
Size: 143 4812
AccI GT'mk_AC
Cuts at: 1993 1993
Size: 4955
Acc65I G'GTAC_C
Cuts at: 899 1971 899
Size: 1072 3883
AclI AA'CG_TT
Cuts at: 920 4795 920
Size: 3875 1080
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 3212 3959 4227 2780
Size: 432 747 268 3508
Fragments arranged by size:
3508 747 432 268
AflII C'TTAA_G
Cuts at: 565 1576 565
Size: 1011 3944
AflIII A'CryG_T
Cuts at: 2307 3411 4388 2307
Size: 1104 977 2874
Fragments arranged by size:
2874 1104 977
AgeI A'CCGG_T
Cuts at: 3343 3343
Size: 4955
AleI CACnn'nnGTG
Cuts at: 271 271
Size: 4955
AlwNI CAG_nnn'CTG
Cuts at: 156 388 2260 3827 156
Size: 232 1872 1567 1284
Fragments arranged by size:
1872 1567 1284 232
ApaLI G'TGCA_C
Cuts at: 1246 3725 1246
Size: 2479 2476
ApoI r'AATT_y
Cuts at: 420 1108 1121 1135 1959 4308 4564 420
Size: 688 13 14 824 2349 256 811
Fragments arranged by size:
2349 824 811 688 256 14 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 4955
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
AvaI C'yCGr_G
Cuts at: 1975 1975
Size: 4955
BamHI G'GATC_C
Cuts at: 1980 1980
Size: 4955
BanI G'GyrC_C
Cuts at: 241 899 1907 1971 2385 2633 2668 241
Size: 658 1008 64 414 248 35 2528
Fragments arranged by size:
2528 1008 658 414 248 64 35
BanII G_rGCy'C
Cuts at: 815 1969 2999 815
Size: 1154 1030 2771
Fragments arranged by size:
2771 1154 1030
BbeI G_GCGC'C
Cuts at: 2389 2637 2389
Size: 248 4707
BbsI GAAGACnn'nnnn_
Cuts at: 416 416
Size: 4955
BciVI GTATCCnnnnn_n'
Cuts at: 2853 3614 4955 2853
Size: 761 1341 2853
Fragments arranged by size:
2853 1341 761
BclI T'GATC_A
Cuts at: 2475 2475
Size: 4955
BglII A'GATC_T
Cuts at: 2470 4507 2470
Size: 2037 2918
BlpI GC'TnA_GC
Cuts at: 2067 2067
Size: 4955
Bme1580I G_kGCm'C
Cuts at: 877 1250 1355 2580 2673 3729 877
Size: 373 105 1225 93 1056 2103
Fragments arranged by size:
2103 1225 1056 373 105 93
BmgBI CAC'GTC
Cuts at: 393 393
Size: 4955
BmrI ACTGGGnnnn_n'
Cuts at: 1663 2167 2248 2583 4727 1663
Size: 504 81 335 2144 1891
Fragments arranged by size:
2144 1891 504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3322 3322
Size: 4955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1286 1454 1805 1829 925
Size: 361 168 351 24 4051
Fragments arranged by size:
4051 361 351 168 24
Bpu10I CC'TnA_GC
Cuts at: 785 785
Size: 4955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2131 3010 3502 3800 1225
Size: 906 879 492 298 2380
Fragments arranged by size:
2380 906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4659 4659
Size: 4955
BsaAI yAC'GTr
Cuts at: 1947 2308 1947
Size: 361 4594
BsaBI GATnn'nnATC
Cuts at: 6 2469 4506 6
Size: 2463 2037 455
Fragments arranged by size:
2463 2037 455
BsaHI Gr'CG_yC
Cuts at: 2386 2634 3336 4832 2386
Size: 248 702 1496 2509
Fragments arranged by size:
2509 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2144 2665 3343 3617 3764 4175 2144
Size: 521 678 274 147 411 2924
Fragments arranged by size:
2924 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 323 353 323
Size: 30 4925
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 595 451
Size: 144 4811
BseYI C'CCAG_C
Cuts at: 1659 2380 3138 3715 4211 1659
Size: 721 758 577 496 2403
Fragments arranged by size:
2403 758 721 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 1759
Size: 4955
BsiEI CG_ry'CG
Cuts at: 104 2497 2543 3751 104
Size: 2393 46 1208 1308
Fragments arranged by size:
2393 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1250 1969 2747 2937 3729 1250
Size: 719 778 190 792 2476
Fragments arranged by size:
2476 792 778 719 190
BsmI GAATG_Cn'
Cuts at: 1260 1340 1260
Size: 80 4875
Bsp1286I G_dGCh'C
Cuts at: 815 877 1250 1355 1969 2580 2673 2747 2937
Size: 62 373 105 614 611 93 74 190
Cuts at: 2937 2999 3729 815
Size: 62 730 2041
Fragments arranged by size:
2041 730 614 611 373 190 105 93
74 62 62
BspEI T'CCGG_A
Cuts at: 4175 4175
Size: 4955
BspHI T'CATG_A
Cuts at: 1068 4131 1068
Size: 3063 1892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1638 1781 2005 2219 2521 2902 1638
Size: 143 224 214 302 381 3691
Fragments arranged by size:
3691 381 302 224 214 143
BsrBI CCG'CTC
Cuts at: 50 3247 3301 4785 4950 50
Size: 3197 54 1484 165 55
Fragments arranged by size:
3197 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 630 2867 630
Size: 2237 2718
BsrFI r'CCGG_y
Cuts at: 2953 3134 3343 2953
Size: 181 209 4565
Fragments arranged by size:
4565 209 181
BsrGI T'GTAC_A
Cuts at: 275 275
Size: 4955
BssSI C'ACGA_G
Cuts at: 3226 3584 3226
Size: 358 4597
BstAPI GCAn_nnn'nTGC
Cuts at: 1254 2332 1254
Size: 1078 3877
BstBI TT'CG_AA
Cuts at: 110 3316 110
Size: 3206 1749
BstEII G'GTnAC_C
Cuts at: 1605 1605
Size: 4955
BstXI CCAn_nnnn'nTGG
Cuts at: 1378 1378
Size: 4955
BstYI r'GATC_y
Cuts at: 1 1640 1980 2447 2470 2805 3051 4063 4149
Size: 1639 340 467 23 335 246 1012 86
Cuts at: 4149 4161 4507 1
Size: 12 346 449
Fragments arranged by size:
1639 1012 467 449 346 340 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1089 1145 1089
Size: 56 4899
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2905 3086 3249 2905
Size: 181 163 4611
Fragments arranged by size:
4611 181 163
BtsI GCAGTG_nn'
Cuts at: 389 582 2198 389
Size: 193 1616 3146
Fragments arranged by size:
3146 1616 193
DraI TTT'AAA
Cuts at: 1871 1954 1871
Size: 83 4872
DrdI GACnn_nn'nnGTC
Cuts at: 2661 3519 2661
Size: 858 4097
EaeI y'GGCC_r
Cuts at: 1548 2540 2714 3105 3132 4477 1548
Size: 992 174 391 27 1345 2026
Fragments arranged by size:
2026 1345 992 391 174 27
EagI C'GGCC_G
Cuts at: 2540 2540
Size: 4955
EarI CTCTTCn'nnn_
Cuts at: 437 543 581 587 599 763 839 1489 1742
Size: 106 38 6 12 164 76 650 253
Cuts at: 1742 2978 3188 437
Size: 1236 210 2204
Fragments arranged by size:
2204 1236 650 253 210 164 106 76
38 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3163 3473 3619 4765 3163
Size: 310 146 1146 3353
Fragments arranged by size:
3353 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1967 1967
Size: 4955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1286 1454 1805 1829 2780 3212 3959 4227
Size: 361 168 351 24 951 432 747 268
Cuts at: 4227 925
Size: 1653
Fragments arranged by size:
1653 951 747 432 361 351 268 168
24
EcoO109I rG'GnC_Cy
Cuts at: 2094 2094
Size: 4955
EcoRI G'AATT_C
Cuts at: 420 1959 420
Size: 1539 3416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1422 2974 1422
Size: 1552 3403
FspI TGC'GCA
Cuts at: 2736 2736
Size: 4955
HaeII r_GCGC'y
Cuts at: 2389 2637 3659 4638 2389
Size: 248 1022 979 2706
Fragments arranged by size:
2706 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2797 2827 2829 2859 2797
Size: 30 2 30 4893
Fragments arranged by size:
4893 30 30 2
HincII GTy'rAC
Cuts at: 1994 1994
Size: 4955
HindIII A'AGCT_T
Cuts at: 1470 1470
Size: 4955
KasI G'GCGC_C
Cuts at: 2385 2633 2385
Size: 248 4707
KpnI G_GTAC'C
Cuts at: 903 1975 903
Size: 1072 3883
MluI A'CGCG_T
Cuts at: 4388 4388
Size: 4955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3626 3810 3626
Size: 184 4771
MscI TGG'CCA
Cuts at: 1550 2716 1550
Size: 1166 3789
MslI CAynn'nnrTG
Cuts at: 271 322 341 1094 1197 2944 3071 271
Size: 51 19 753 103 1747 127 2155
Fragments arranged by size:
2155 1747 753 127 103 51 19
MspA1I CmG'CkG
Cuts at: 1554 2066 2380 2740 3753 3998 1554
Size: 512 314 360 1013 245 2511
Fragments arranged by size:
2511 1013 512 360 314 245
NaeI GCC'GGC
Cuts at: 3136 3136
Size: 4955
NarI GG'CG_CC
Cuts at: 2386 2634 2386
Size: 248 4707
NcoI C'CATG_G
Cuts at: 1089 1145 1089
Size: 56 4899
NgoMIV G'CCGG_C
Cuts at: 3134 3134
Size: 4955
NspI r_CATG'y
Cuts at: 181 400 939 1191 1202 1575 2008 2508 3415
Size: 219 539 252 11 373 433 500 907
Cuts at: 3415 181
Size: 1721
Fragments arranged by size:
1721 907 539 500 433 373 252 219
11
PasI CC'CwG_GG
Cuts at: 878 878
Size: 4955
PciI A'CATG_T
Cuts at: 3411 3411
Size: 4955
PfoI T'CCnGG_A
Cuts at: 971 971
Size: 4955
PmeI GTTT'AAAC
Cuts at: 1954 1954
Size: 4955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 4923
PstI C_TGCA'G
Cuts at: 2002 2687 2002
Size: 685 4270
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4955
PvuII CAG'CTG
Cuts at: 2380 2740 2380
Size: 360 4595
RsrII CG'GwC_CG
Cuts at: 3150 3150
Size: 4955
SacI G_AGCT'C
Cuts at: 1969 1969
Size: 4955
SalI G'TCGA_C
Cuts at: 1992 1992
Size: 4955
SapI GCTCTTCn'nnn_
Cuts at: 2978 3188 2978
Size: 210 4745
SbfI CC_TGCA'GG
Cuts at: 2002 2002
Size: 4955
ScaI AGT'ACT
Cuts at: 1405 4472 1405
Size: 3067 1888
SfcI C'TryA_G
Cuts at: 33 1998 2683 3676 3867 33
Size: 1965 685 993 191 1121
Fragments arranged by size:
1965 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2387 2635 2387
Size: 248 4707
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
SmaI CCC'GGG
Cuts at: 1977 1977
Size: 4955
SmlI C'TyrA_G
Cuts at: 565 1240 1576 2110 3025 3517 3779 565
Size: 675 336 534 915 492 262 1741
Fragments arranged by size:
1741 915 675 534 492 336 262
SnaBI TAC'GTA
Cuts at: 1947 1947
Size: 4955
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4955
SphI G_CATG'C
Cuts at: 400 939 2008 2508 400
Size: 539 1069 500 2847
Fragments arranged by size:
2847 1069 539 500
StyI C'CwwG_G
Cuts at: 1089 1145 2089 2443 1089
Size: 56 944 354 3601
Fragments arranged by size:
3601 944 354 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1799 1814 2538 2671 3130 3339 1799
Size: 15 724 133 459 209 3415
Fragments arranged by size:
3415 724 459 209 133 15
TatI w'GTAC_w
Cuts at: 275 1310 1403 4470 275
Size: 1035 93 3067 760
Fragments arranged by size:
3067 1035 760 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1619 1651 1656 1688 4472 4504 1619
Size: 32 5 32 2784 32 2070
Fragments arranged by size:
2784 2070 32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 2752 2752
Size: 4955
XbaI T'CTAG_A
Cuts at: 1986 1986
Size: 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2450 2450
Size: 4955
XmaI C'CCGG_G
Cuts at: 1975 1975
Size: 4955
XmnI GAAnn'nnTTC
Cuts at: 1135 1304 1135
Size: 169 4786
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI BsmBI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI