Restriction map for
pF1KSDA0893
(Circular) (Six-base) MAPSORT of: pF1KSDA0893.seq Check: 9686 from: 1 to: 5896
pF1KSDA0893 5896 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2932 2932
Size: 5896
Acc65I G'GTAC_C
Cuts at: 2910 2910
Size: 5896
AclI AA'CG_TT
Cuts at: 5736 5736
Size: 5896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 304 406 853 1805 3719 4151 4900 5168
Size: 149 102 447 952 1914 432 749 268
Cuts at: 5168 155
Size: 883
Fragments arranged by size:
1914 952 883 749 447 432 268 149
102
AflIII A'CryG_T
Cuts at: 3246 4352 5329 3246
Size: 1106 977 3813
Fragments arranged by size:
3813 1106 977
AgeI A'CCGG_T
Cuts at: 4284 4284
Size: 5896
AlwNI CAG_nnn'CTG
Cuts at: 453 3199 4768 453
Size: 2746 1569 1581
Fragments arranged by size:
2746 1581 1569
ApaLI G'TGCA_C
Cuts at: 2051 4666 2051
Size: 2615 3281
ApoI r'AATT_y
Cuts at: 192 352 457 646 751 1115 2001 2233 2898
Size: 160 105 189 105 364 886 232 665
Cuts at: 2898 5249 5505 192
Size: 2351 256 583
Fragments arranged by size:
2351 886 665 583 364 256 232 189
160 105 105
AseI AT'TA_AT
Cuts at: 20 1925 20
Size: 1905 3991
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5896
AvaI C'yCGr_G
Cuts at: 2914 2914
Size: 5896
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1250 1283 1250
Size: 33 5863
BamHI G'GATC_C
Cuts at: 2110 2919 2110
Size: 809 5087
BanI G'GyrC_C
Cuts at: 2910 3324 3572 3607 2910
Size: 414 248 35 5199
Fragments arranged by size:
5199 414 248 35
BanII G_rGCy'C
Cuts at: 446 2688 2908 3938 446
Size: 2242 220 1030 2404
Fragments arranged by size:
2404 2242 1030 220
BbeI G_GCGC'C
Cuts at: 3328 3576 3328
Size: 248 5648
BbsI GAAGACnn'nnnn_
Cuts at: 882 1032 1943 882
Size: 150 911 4835
Fragments arranged by size:
4835 911 150
BciVI GTATCCnnnnn_n'
Cuts at: 3792 4555 5896 3792
Size: 763 1341 3792
Fragments arranged by size:
3792 1341 763
BclI T'GATC_A
Cuts at: 1521 3414 1521
Size: 1893 4003
BfrBI ATG'CAT
Cuts at: 2244 2244
Size: 5896
BglII A'GATC_T
Cuts at: 2226 3409 5448 2226
Size: 1183 2039 2674
Fragments arranged by size:
2674 2039 1183
BlpI GC'TnA_GC
Cuts at: 3006 3006
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 2055 3519 3612 4670 2055
Size: 1464 93 1058 3281
Fragments arranged by size:
3281 1464 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2399 2549 3106 3187 3522 5668 2399
Size: 150 557 81 335 2146 2627
Fragments arranged by size:
2627 2146 557 335 150 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1046 4261 1046
Size: 3215 2681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 794 1810 1844 1997 2151 2336 2436 2702
Size: 547 1016 34 153 154 185 100 266
Cuts at: 2702 247
Size: 3441
Fragments arranged by size:
3441 1016 547 266 185 154 153 100
34
Bpu10I CC'TnA_GC
Cuts at: 1250 2066 1250
Size: 816 5080
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 3070 3949 4443 4741 1231
Size: 1839 879 494 298 2386
Fragments arranged by size:
2386 1839 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5600 5600
Size: 5896
BsaAI yAC'GTr
Cuts at: 2886 3247 2886
Size: 361 5535
BsaBI GATnn'nnATC
Cuts at: 6 845 3408 5447 6
Size: 839 2563 2039 455
Fragments arranged by size:
2563 2039 839 455
BsaHI Gr'CG_yC
Cuts at: 3325 3573 4277 5773 3325
Size: 248 704 1496 3448
Fragments arranged by size:
3448 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3083 3604 4284 4558 4705 5116 3083
Size: 521 680 274 147 411 3863
Fragments arranged by size:
3863 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1734 1764 2124 2154 1734
Size: 30 360 30 5476
Fragments arranged by size:
5476 360 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 971 1742 2309 2630 971
Size: 771 567 321 4237
Fragments arranged by size:
4237 771 567 321
BseYI C'CCAG_C
Cuts at: 446 595 3319 4077 4656 5152 446
Size: 149 2724 758 579 496 1190
Fragments arranged by size:
2724 1190 758 579 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2549 2826 2549
Size: 277 5619
BsiEI CG_ry'CG
Cuts at: 104 3436 3482 4692 104
Size: 3332 46 1210 1308
Fragments arranged by size:
3332 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1344 2055 2688 2786 2908 3686 3876 4670 1344
Size: 711 633 98 122 778 190 794 2570
Fragments arranged by size:
2570 794 778 711 633 190 122 98
BsmI GAATG_Cn'
Cuts at: 745 745
Size: 5896
BsmBI CGTCTCn'nnnn_
Cuts at: 2569 2569
Size: 5896
Bsp1286I G_dGCh'C
Cuts at: 446 1344 2055 2688 2786 2908 3519 3612 3686
Size: 898 711 633 98 122 611 93 74
Cuts at: 3686 3876 3938 4670 446
Size: 190 62 732 1672
Fragments arranged by size:
1672 898 732 711 633 611 190 122
98 93 74 62
BspEI T'CCGG_A
Cuts at: 5116 5116
Size: 5896
BspHI T'CATG_A
Cuts at: 1104 5072 1104
Size: 3968 1928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2944 3158 3460 3841 2944
Size: 214 302 381 4999
Fragments arranged by size:
4999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1058 4186 4240 5726 5891 50
Size: 1008 3128 54 1486 165 55
Fragments arranged by size:
3128 1486 1008 165 55 54
BsrDI GCAATG_nn'
Cuts at: 487 1639 1659 3806 487
Size: 1152 20 2147 2577
Fragments arranged by size:
2577 2147 1152 20
BsrFI r'CCGG_y
Cuts at: 2073 3892 4073 4284 2073
Size: 1819 181 211 3685
Fragments arranged by size:
3685 1819 211 181
BssSI C'ACGA_G
Cuts at: 605 4165 4525 605
Size: 3560 360 1976
Fragments arranged by size:
3560 1976 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3271 3271
Size: 5896
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5896
BstYI r'GATC_y
Cuts at: 1 846 1657 2110 2226 2469 2550 2823 2919
Size: 845 811 453 116 243 81 273 96
Cuts at: 2919 3386 3409 3744 3990 5004 5090 5102 5448
Size: 467 23 335 246 1014 86 12 346
Cuts at: 5448 1
Size: 449
Fragments arranged by size:
1014 845 811 467 453 449 346 335
273 246 243 116 96 86 81 23
12
Bsu36I CC'TnA_GG
Cuts at: 280 280
Size: 5896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 439 3844 4025 4188 439
Size: 3405 181 163 2147
Fragments arranged by size:
3405 2147 181 163
BtsI GCAGTG_nn'
Cuts at: 1296 1964 2532 2537 3137 1296
Size: 668 568 5 600 4055
Fragments arranged by size:
4055 668 600 568 5
DraI TTT'AAA
Cuts at: 2088 2893 2088
Size: 805 5091
DraIII CAC_nnn'GTG
Cuts at: 1804 1804
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 1100 2176 3600 4460 1100
Size: 1076 1424 860 2536
Fragments arranged by size:
2536 1424 1076 860
EaeI y'GGCC_r
Cuts at: 3479 3653 4044 4071 5418 3479
Size: 174 391 27 1347 3957
Fragments arranged by size:
3957 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3479 3479
Size: 5896
EarI CTCTTCn'nnn_
Cuts at: 617 1824 1883 3917 4127 617
Size: 1207 59 2034 210 2386
Fragments arranged by size:
2386 2034 1207 210 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4102 4414 4560 5706 4102
Size: 312 146 1146 4292
Fragments arranged by size:
4292 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2686 2906 2686
Size: 220 5676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 247 304 406 794 853 1805 1810 1844
Size: 92 57 102 388 59 952 5 34
Cuts at: 1844 1997 2151 2336 2436 2702 3719 4151 4900
Size: 153 154 185 100 266 1017 432 749
Cuts at: 4900 5168 155
Size: 268 883
Fragments arranged by size:
1017 952 883 749 432 388 268 266
185 154 153 102 100 92 59 57
34 5
EcoNI CCTnn'n_nnAGG
Cuts at: 229 229
Size: 5896
EcoO109I rG'GnC_Cy
Cuts at: 2364 2745 3033 2364
Size: 381 288 5227
Fragments arranged by size:
5227 381 288
EcoRI G'AATT_C
Cuts at: 192 646 2898 192
Size: 454 2252 3190
Fragments arranged by size:
3190 2252 454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 748 820 3913 748
Size: 72 3093 2731
Fragments arranged by size:
3093 2731 72
FspI TGC'GCA
Cuts at: 3675 3675
Size: 5896
HaeII r_GCGC'y
Cuts at: 3328 3576 4600 5579 3328
Size: 248 1024 979 3645
Fragments arranged by size:
3645 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 540 572 1044 1076 1885 1917 2647 2679 3736
Size: 32 472 32 809 32 730 32 1057
Cuts at: 3736 3766 3768 3798 540
Size: 30 2 30 2638
Fragments arranged by size:
2638 1057 809 730 472 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 417 933 2933 417
Size: 516 2000 3380
Fragments arranged by size:
3380 2000 516
HindIII A'AGCT_T
Cuts at: 202 404 1570 202
Size: 202 1166 4528
Fragments arranged by size:
4528 1166 202
HpaI GTT'AAC
Cuts at: 417 417
Size: 5896
KasI G'GCGC_C
Cuts at: 3324 3572 3324
Size: 248 5648
KpnI G_GTAC'C
Cuts at: 2914 2914
Size: 5896
MluI A'CGCG_T
Cuts at: 5329 5329
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4567 4751 4567
Size: 184 5712
MscI TGG'CCA
Cuts at: 3655 3655
Size: 5896
MslI CAynn'nnrTG
Cuts at: 214 2651 2773 3883 4010 214
Size: 2437 122 1110 127 2100
Fragments arranged by size:
2437 2100 1110 127 122
MspA1I CmG'CkG
Cuts at: 1039 1464 1975 3005 3319 3679 4694 4939 1039
Size: 425 511 1030 314 360 1015 245 1996
Fragments arranged by size:
1996 1030 1015 511 425 360 314 245
NaeI GCC'GGC
Cuts at: 4075 4075
Size: 5896
NarI GG'CG_CC
Cuts at: 3325 3573 3325
Size: 248 5648
NdeI CA'TA_TG
Cuts at: 963 963
Size: 5896
NgoMIV G'CCGG_C
Cuts at: 4073 4073
Size: 5896
NruI TCG'CGA
Cuts at: 4257 4257
Size: 5896
NsiI A_TGCA'T
Cuts at: 2246 2246
Size: 5896
NspI r_CATG'y
Cuts at: 493 2601 2947 3447 4356 493
Size: 2108 346 500 909 2033
Fragments arranged by size:
2108 2033 909 500 346
PciI A'CATG_T
Cuts at: 4352 4352
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 453 1849 453
Size: 1396 4500
PfoI T'CCnGG_A
Cuts at: 1350 1761 1350
Size: 411 5485
PmeI GTTT'AAAC
Cuts at: 2893 2893
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5035 5067 5035
Size: 32 5864
PpuMI rG'GwC_Cy
Cuts at: 2745 2745
Size: 5896
PshAI GACnn'nnGTC
Cuts at: 1706 1706
Size: 5896
PstI C_TGCA'G
Cuts at: 2850 2941 3626 2850
Size: 91 685 5120
Fragments arranged by size:
5120 685 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5896
PvuII CAG'CTG
Cuts at: 1039 1975 3319 3679 1039
Size: 936 1344 360 3256
Fragments arranged by size:
3256 1344 936 360
RsrII CG'GwC_CG
Cuts at: 4089 4089
Size: 5896
SacI G_AGCT'C
Cuts at: 2688 2908 2688
Size: 220 5676
SalI G'TCGA_C
Cuts at: 2931 2931
Size: 5896
SapI GCTCTTCn'nnn_
Cuts at: 3917 4127 3917
Size: 210 5686
SbfI CC_TGCA'GG
Cuts at: 2941 2941
Size: 5896
ScaI AGT'ACT
Cuts at: 5413 5413
Size: 5896
SfcI C'TryA_G
Cuts at: 33 525 2540 2546 2846 2937 3622 4617 4808
Size: 492 2015 6 300 91 685 995 191
Cuts at: 4808 33
Size: 1121
Fragments arranged by size:
2015 1121 995 685 492 300 191 91
6
SfoI GGC'GCC
Cuts at: 3326 3574 3326
Size: 248 5648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5896
SmaI CCC'GGG
Cuts at: 2916 2916
Size: 5896
SmlI C'TyrA_G
Cuts at: 1210 3049 3964 4458 4720 1210
Size: 1839 915 494 262 2386
Fragments arranged by size:
2386 1839 915 494 262
SnaBI TAC'GTA
Cuts at: 2886 2886
Size: 5896
SpeI A'CTAG_T
Cuts at: 67 1678 67
Size: 1611 4285
SphI G_CATG'C
Cuts at: 2947 3447 2947
Size: 500 5396
SspI AAT'ATT
Cuts at: 924 1411 1587 1737 924
Size: 487 176 150 5083
Fragments arranged by size:
5083 487 176 150
StuI AGG'CCT
Cuts at: 728 1281 728
Size: 553 5343
StyI C'CwwG_G
Cuts at: 1125 1602 3028 3382 1125
Size: 477 1426 354 3639
Fragments arranged by size:
3639 1426 477 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1821 2833 3477 3610 4069 4280 1821
Size: 1012 644 133 459 211 3437
Fragments arranged by size:
3437 1012 644 459 211 133
TatI w'GTAC_w
Cuts at: 5411 5411
Size: 5896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 657 689 2124 2156 5413 5445 657
Size: 32 1435 32 3257 32 1108
Fragments arranged by size:
3257 1435 1108 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3691 3691
Size: 5896
XbaI T'CTAG_A
Cuts at: 336 495 2925 336
Size: 159 2430 3307
Fragments arranged by size:
3307 2430 159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1636 2121 2377 3389 1636
Size: 485 256 1012 4143
Fragments arranged by size:
4143 1012 485 256
XmaI C'CCGG_G
Cuts at: 2914 2914
Size: 5896
XmnI GAAnn'nnTTC
Cuts at: 317 800 1400 317
Size: 483 600 4813
Fragments arranged by size:
4813 600 483
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NruI NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BglI BmgBI BmtI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI EcoRV FseI FspAI MfeI NcoI NheI NotI
PacI PasI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SwaI XhoI ZraI