Restriction map for
pF1KSDA0873
(Circular) (Six-base) MAPSORT of: pF1KSDA0873.seq Check: 2290 from: 1 to: 4480
pF1KSDA0873 4480 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1516 1516
Size: 4480
Acc65I G'GTAC_C
Cuts at: 1494 1494
Size: 4480
AclI AA'CG_TT
Cuts at: 4320 4320
Size: 4480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 198 320 2303 2735 3484 3752 148
Size: 50 122 1983 432 749 268 876
Fragments arranged by size:
1983 876 749 432 268 122 50
AflIII A'CryG_T
Cuts at: 531 1830 2936 3913 531
Size: 1299 1106 977 1098
Fragments arranged by size:
1299 1106 1098 977
AgeI A'CCGG_T
Cuts at: 2868 2868
Size: 4480
AhdI GACnn_n'nnGTC
Cuts at: 353 353
Size: 4480
AlwNI CAG_nnn'CTG
Cuts at: 1783 3352 1783
Size: 1569 2911
ApaLI G'TGCA_C
Cuts at: 3250 3250
Size: 4480
ApoI r'AATT_y
Cuts at: 1482 3833 4089 1482
Size: 2351 256 1873
Fragments arranged by size:
2351 1873 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4480
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4480
AvaI C'yCGr_G
Cuts at: 713 786 1498 713
Size: 73 712 3695
Fragments arranged by size:
3695 712 73
BamHI G'GATC_C
Cuts at: 1503 1503
Size: 4480
BanI G'GyrC_C
Cuts at: 1494 1908 2156 2191 1494
Size: 414 248 35 3783
Fragments arranged by size:
3783 414 248 35
BanII G_rGCy'C
Cuts at: 1042 1492 2522 1042
Size: 450 1030 3000
Fragments arranged by size:
3000 1030 450
BbeI G_GCGC'C
Cuts at: 1912 2160 1912
Size: 248 4232
BbsI GAAGACnn'nnnn_
Cuts at: 349 623 349
Size: 274 4206
BciVI GTATCCnnnnn_n'
Cuts at: 2376 3139 4480 2376
Size: 763 1341 2376
Fragments arranged by size:
2376 1341 763
BclI T'GATC_A
Cuts at: 1151 1998 1151
Size: 847 3633
BglII A'GATC_T
Cuts at: 1993 4032 1993
Size: 2039 2441
BlpI GC'TnA_GC
Cuts at: 1590 1590
Size: 4480
Bme1580I G_kGCm'C
Cuts at: 2103 2196 3254 2103
Size: 93 1058 3329
Fragments arranged by size:
3329 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 423 1690 1771 2106 4252 423
Size: 1267 81 335 2146 651
Fragments arranged by size:
2146 1267 651 335 81
BmtI G_CTAG'C
Cuts at: 1348 1348
Size: 4480
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2845 2845
Size: 4480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 563 271
Size: 292 4188
Bpu10I CC'TnA_GC
Cuts at: 1036 1036
Size: 4480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1201 1654 2533 3027 3325 958
Size: 243 453 879 494 298 2113
Fragments arranged by size:
2113 879 494 453 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 751 4184 751
Size: 3433 1047
BsaAI yAC'GTr
Cuts at: 1470 1831 1470
Size: 361 4119
BsaBI GATnn'nnATC
Cuts at: 6 662 1289 1992 4031 6
Size: 656 627 703 2039 455
Fragments arranged by size:
2039 703 656 627 455
BsaHI Gr'CG_yC
Cuts at: 1909 2157 2861 4357 1909
Size: 248 704 1496 2032
Fragments arranged by size:
2032 1496 704 248
BsaWI w'CCGG_w
Cuts at: 395 1667 2188 2868 3142 3289 3700 395
Size: 1272 521 680 274 147 411 1175
Fragments arranged by size:
1272 1175 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1024 1054 1024
Size: 30 4450
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1222 1222
Size: 4480
BseYI C'CCAG_C
Cuts at: 1903 2661 3240 3736 1903
Size: 758 579 496 2647
Fragments arranged by size:
2647 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 828 2020 2066 3276 104
Size: 724 1192 46 1210 1308
Fragments arranged by size:
1308 1210 1192 724 46
BsiHKAI G_wGCw'C
Cuts at: 785 1042 1214 1492 2270 2460 3254 785
Size: 257 172 278 778 190 794 2011
Fragments arranged by size:
2011 794 778 278 257 190 172
BsmI GAATG_Cn'
Cuts at: 384 384
Size: 4480
Bsp1286I G_dGCh'C
Cuts at: 785 1042 1214 1492 2103 2196 2270 2460 2522
Size: 257 172 278 611 93 74 190 62
Cuts at: 2522 3254 785
Size: 732 2011
Fragments arranged by size:
2011 732 611 278 257 190 172 93
74 62
BspEI T'CCGG_A
Cuts at: 3700 3700
Size: 4480
BspHI T'CATG_A
Cuts at: 3656 3656
Size: 4480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 769 1528 1742 2044 2425 769
Size: 759 214 302 381 2824
Fragments arranged by size:
2824 759 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2770 2824 4310 4475 50
Size: 2720 54 1486 165 55
Fragments arranged by size:
2720 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2390 2390
Size: 4480
BsrFI r'CCGG_y
Cuts at: 2476 2657 2868 2476
Size: 181 211 4088
Fragments arranged by size:
4088 211 181
BssSI C'ACGA_G
Cuts at: 436 2749 3109 436
Size: 2313 360 1807
Fragments arranged by size:
2313 1807 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1855 1855
Size: 4480
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4480
BstXI CCAn_nnnn'nTGG
Cuts at: 132 132
Size: 4480
BstYI r'GATC_y
Cuts at: 1 1259 1503 1970 1993 2328 2574 3588 3674
Size: 1258 244 467 23 335 246 1014 86
Cuts at: 3674 3686 4032 1
Size: 12 346 449
Fragments arranged by size:
1258 1014 467 449 346 335 246 244
86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2428 2609 2772 2428
Size: 181 163 4136
Fragments arranged by size:
4136 181 163
BtsI GCAGTG_nn'
Cuts at: 1136 1721 1136
Size: 585 3895
DraI TTT'AAA
Cuts at: 956 1477 956
Size: 521 3959
DrdI GACnn_nn'nnGTC
Cuts at: 2184 3044 2184
Size: 860 3620
EaeI y'GGCC_r
Cuts at: 825 2063 2237 2628 2655 4002 825
Size: 1238 174 391 27 1347 1303
Fragments arranged by size:
1347 1303 1238 391 174 27
EagI C'GGCC_G
Cuts at: 825 2063 825
Size: 1238 3242
EarI CTCTTCn'nnn_
Cuts at: 896 1119 1316 1370 2501 2711 896
Size: 223 197 54 1131 210 2665
Fragments arranged by size:
2665 1131 223 210 197 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2686 2998 3144 4290 2686
Size: 312 146 1146 2876
Fragments arranged by size:
2876 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1040 1490 1040
Size: 450 4030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 198 271 320 563 2303 2735 3484 3752
Size: 50 73 49 243 1740 432 749 268
Cuts at: 3752 148
Size: 876
Fragments arranged by size:
1740 876 749 432 268 243 73 50
49
EcoO109I rG'GnC_Cy
Cuts at: 720 969 1176 1617 720
Size: 249 207 441 3583
Fragments arranged by size:
3583 441 249 207
EcoRI G'AATT_C
Cuts at: 1482 1482
Size: 4480
EcoRV GAT'ATC
Cuts at: 513 870 513
Size: 357 4123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4480
FspI TGC'GCA
Cuts at: 1418 2259 1418
Size: 841 3639
HaeII r_GCGC'y
Cuts at: 1912 2160 3184 4163 1912
Size: 248 1024 979 2229
Fragments arranged by size:
2229 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 339 371 536 568 927 959 1118 1150 1201
Size: 32 165 32 359 32 159 32 51
Cuts at: 1201 1233 1432 1464 2320 2350 2352 2382 339
Size: 32 199 32 856 30 2 30 2437
Fragments arranged by size:
2437 856 359 199 165 159 51 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1517 1517
Size: 4480
KasI G'GCGC_C
Cuts at: 1908 2156 1908
Size: 248 4232
KpnI G_GTAC'C
Cuts at: 1498 1498
Size: 4480
MluI A'CGCG_T
Cuts at: 3913 3913
Size: 4480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 1055 3151 3335 350
Size: 705 2096 184 1495
Fragments arranged by size:
2096 1495 705 184
MscI TGG'CCA
Cuts at: 2239 2239
Size: 4480
MslI CAynn'nnrTG
Cuts at: 130 2467 2594 130
Size: 2337 127 2016
Fragments arranged by size:
2337 2016 127
MspA1I CmG'CkG
Cuts at: 1341 1589 1903 2263 3278 3523 1341
Size: 248 314 360 1015 245 2298
Fragments arranged by size:
2298 1015 360 314 248 245
NaeI GCC'GGC
Cuts at: 2659 2659
Size: 4480
NarI GG'CG_CC
Cuts at: 1909 2157 1909
Size: 248 4232
NcoI C'CATG_G
Cuts at: 125 125
Size: 4480
NgoMIV G'CCGG_C
Cuts at: 2657 2657
Size: 4480
NheI G'CTAG_C
Cuts at: 1344 1344
Size: 4480
NruI TCG'CGA
Cuts at: 2841 2841
Size: 4480
NspI r_CATG'y
Cuts at: 535 1531 2031 2940 535
Size: 996 500 909 2075
Fragments arranged by size:
2075 996 909 500
PciI A'CATG_T
Cuts at: 531 2936 531
Size: 2405 2075
PfoI T'CCnGG_A
Cuts at: 1109 1109
Size: 4480
PmeI GTTT'AAAC
Cuts at: 1477 1477
Size: 4480
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 344 376 3619 3651 344
Size: 32 3243 32 1173
Fragments arranged by size:
3243 1173 32 32
PpuMI rG'GwC_Cy
Cuts at: 720 969 720
Size: 249 4231
PsiI TTA'TAA
Cuts at: 464 464
Size: 4480
PstI C_TGCA'G
Cuts at: 766 1525 2210 766
Size: 759 685 3036
Fragments arranged by size:
3036 759 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4480
PvuII CAG'CTG
Cuts at: 1341 1903 2263 1341
Size: 562 360 3558
Fragments arranged by size:
3558 562 360
RsrII CG'GwC_CG
Cuts at: 2673 2673
Size: 4480
SacI G_AGCT'C
Cuts at: 1042 1492 1042
Size: 450 4030
SalI G'TCGA_C
Cuts at: 1515 1515
Size: 4480
SapI GCTCTTCn'nnn_
Cuts at: 1119 1316 2501 2711 1119
Size: 197 1185 210 2888
Fragments arranged by size:
2888 1185 210 197
SbfI CC_TGCA'GG
Cuts at: 766 1525 766
Size: 759 3721
ScaI AGT'ACT
Cuts at: 1465 3997 1465
Size: 2532 1948
SfcI C'TryA_G
Cuts at: 33 290 762 1521 2206 3201 3392 33
Size: 257 472 759 685 995 191 1121
Fragments arranged by size:
1121 995 759 685 472 257 191
SfoI GGC'GCC
Cuts at: 1910 2158 1910
Size: 248 4232
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4480
SmaI CCC'GGG
Cuts at: 1500 1500
Size: 4480
SmlI C'TyrA_G
Cuts at: 973 1180 1633 2548 3042 3304 973
Size: 207 453 915 494 262 2149
Fragments arranged by size:
2149 915 494 453 262 207
SnaBI TAC'GTA
Cuts at: 1470 1470
Size: 4480
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4480
SphI G_CATG'C
Cuts at: 1531 2031 1531
Size: 500 3980
SspI AAT'ATT
Cuts at: 1425 1425
Size: 4480
StyI C'CwwG_G
Cuts at: 125 1242 1612 1966 125
Size: 1117 370 354 2639
Fragments arranged by size:
2639 1117 370 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 404 730 1229 2061 2194 2653 2864 404
Size: 326 499 832 133 459 211 2020
Fragments arranged by size:
2020 832 499 459 326 211 133
TatI w'GTAC_w
Cuts at: 1463 3995 1463
Size: 2532 1948
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3997 4029 3997
Size: 32 4448
Tth111I GACn'n_nGTC
Cuts at: 2275 2275
Size: 4480
XbaI T'CTAG_A
Cuts at: 813 1509 813
Size: 696 3784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1973 1973
Size: 4480
XmaI C'CCGG_G
Cuts at: 1498 1498
Size: 4480
XmnI GAAnn'nnTTC
Cuts at: 356 356
Size: 4480
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NspI PciI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NotI NsiI PacI PasI PflMI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI