Restriction map for
pF1KSDA0871
(Circular) (Six-base) MAPSORT of: pF1KSDA0871.seq Check: 6945 from: 1 to: 4546
pF1KSDA0871 4546 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1582 1582
Size: 4546
Acc65I G'GTAC_C
Cuts at: 1560 1560
Size: 4546
AclI AA'CG_TT
Cuts at: 4386 4386
Size: 4546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 899 952 2369 2801 3550 3818 899
Size: 53 1417 432 749 268 1627
Fragments arranged by size:
1627 1417 749 432 268 53
AflII C'TTAA_G
Cuts at: 592 592
Size: 4546
AflIII A'CryG_T
Cuts at: 1896 3002 3979 1896
Size: 1106 977 2463
Fragments arranged by size:
2463 1106 977
AgeI A'CCGG_T
Cuts at: 2934 2934
Size: 4546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1364 1364
Size: 4546
AlwNI CAG_nnn'CTG
Cuts at: 1317 1849 3418 1317
Size: 532 1569 2445
Fragments arranged by size:
2445 1569 532
ApaLI G'TGCA_C
Cuts at: 3316 3316
Size: 4546
ApoI r'AATT_y
Cuts at: 214 700 1548 3899 4155 214
Size: 486 848 2351 256 605
Fragments arranged by size:
2351 848 605 486 256
AseI AT'TA_AT
Cuts at: 20 637 20
Size: 617 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4546
AvaI C'yCGr_G
Cuts at: 1564 1564
Size: 4546
BamHI G'GATC_C
Cuts at: 1569 1569
Size: 4546
BanI G'GyrC_C
Cuts at: 1560 1974 2222 2257 1560
Size: 414 248 35 3849
Fragments arranged by size:
3849 414 248 35
BanII G_rGCy'C
Cuts at: 1335 1558 2588 1335
Size: 223 1030 3293
Fragments arranged by size:
3293 1030 223
BbeI G_GCGC'C
Cuts at: 1978 2226 1978
Size: 248 4298
BbsI GAAGACnn'nnnn_
Cuts at: 812 1426 812
Size: 614 3932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 135 169 697 731 135
Size: 34 528 34 3950
Fragments arranged by size:
3950 528 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2442 3205 4546 2442
Size: 763 1341 2442
Fragments arranged by size:
2442 1341 763
BclI T'GATC_A
Cuts at: 674 2064 674
Size: 1390 3156
BfrBI ATG'CAT
Cuts at: 985 985
Size: 4546
BglII A'GATC_T
Cuts at: 579 2059 4098 579
Size: 1480 2039 1027
Fragments arranged by size:
2039 1480 1027
BlpI GC'TnA_GC
Cuts at: 1656 1656
Size: 4546
Bme1580I G_kGCm'C
Cuts at: 2169 2262 3320 2169
Size: 93 1058 3395
Fragments arranged by size:
3395 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1756 1837 2172 4318 1756
Size: 81 335 2146 1984
Fragments arranged by size:
2146 1984 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 259 2911 259
Size: 2652 1894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1234 1285 412
Size: 822 51 3673
Fragments arranged by size:
3673 822 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1321 1720 2599 3093 3391 835
Size: 486 399 879 494 298 1990
Fragments arranged by size:
1990 879 494 486 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 190 4250 190
Size: 4060 486
BsaAI yAC'GTr
Cuts at: 1536 1897 1536
Size: 361 4185
BsaBI GATnn'nnATC
Cuts at: 6 1222 2058 4097 6
Size: 1216 836 2039 455
Fragments arranged by size:
2039 1216 836 455
BsaHI Gr'CG_yC
Cuts at: 139 1975 2223 2927 4423 139
Size: 1836 248 704 1496 262
Fragments arranged by size:
1836 1496 704 262 248
BsaWI w'CCGG_w
Cuts at: 1733 2254 2934 3208 3355 3766 1733
Size: 521 680 274 147 411 2513
Fragments arranged by size:
2513 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1030 1030
Size: 4546
BseYI C'CCAG_C
Cuts at: 1969 2727 3306 3802 1969
Size: 758 579 496 2713
Fragments arranged by size:
2713 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1523
Size: 4546
BsiEI CG_ry'CG
Cuts at: 104 149 2086 2132 3342 104
Size: 45 1937 46 1210 1308
Fragments arranged by size:
1937 1308 1210 46 45
BsiHKAI G_wGCw'C
Cuts at: 455 1335 1558 2336 2526 3320 455
Size: 880 223 778 190 794 1681
Fragments arranged by size:
1681 880 794 778 223 190
BsmI GAATG_Cn'
Cuts at: 954 954
Size: 4546
BsmBI CGTCTCn'nnnn_
Cuts at: 878 878
Size: 4546
Bsp1286I G_dGCh'C
Cuts at: 455 1335 1558 2169 2262 2336 2526 2588 3320
Size: 880 223 611 93 74 190 62 732
Cuts at: 3320 455
Size: 1681
Fragments arranged by size:
1681 880 732 611 223 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3766 3766
Size: 4546
BspHI T'CATG_A
Cuts at: 341 722 3722 341
Size: 381 3000 1165
Fragments arranged by size:
3000 1165 381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1594 1808 2110 2491 1594
Size: 214 302 381 3649
Fragments arranged by size:
3649 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2836 2890 4376 4541 50
Size: 2786 54 1486 165 55
Fragments arranged by size:
2786 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2456 2456
Size: 4546
BsrFI r'CCGG_y
Cuts at: 2542 2723 2934 2542
Size: 181 211 4154
Fragments arranged by size:
4154 211 181
BssSI C'ACGA_G
Cuts at: 2815 3175 2815
Size: 360 4186
BstAPI GCAn_nnn'nTGC
Cuts at: 1921 1921
Size: 4546
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4546
BstYI r'GATC_y
Cuts at: 1 303 579 1569 2036 2059 2394 2640 3654
Size: 302 276 990 467 23 335 246 1014
Cuts at: 3654 3740 3752 4098 1
Size: 86 12 346 449
Fragments arranged by size:
1014 990 467 449 346 335 302 276
246 86 23 12
BtgI C'CryG_G
Cuts at: 191 227 191
Size: 36 4510
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2494 2675 2838 2494
Size: 181 163 4202
Fragments arranged by size:
4202 181 163
BtsI GCAGTG_nn'
Cuts at: 1787 1787
Size: 4546
DraI TTT'AAA
Cuts at: 1172 1543 1172
Size: 371 4175
DraIII CAC_nnn'GTG
Cuts at: 1153 1153
Size: 4546
DrdI GACnn_nn'nnGTC
Cuts at: 2250 3110 2250
Size: 860 3686
EaeI y'GGCC_r
Cuts at: 350 2129 2303 2694 2721 4068 350
Size: 1779 174 391 27 1347 828
Fragments arranged by size:
1779 1347 828 391 174 27
EagI C'GGCC_G
Cuts at: 2129 2129
Size: 4546
EarI CTCTTCn'nnn_
Cuts at: 609 1172 1310 1409 2567 2777 609
Size: 563 138 99 1158 210 2378
Fragments arranged by size:
2378 1158 563 210 138 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2752 3064 3210 4356 2752
Size: 312 146 1146 2942
Fragments arranged by size:
2942 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1333 1556 1333
Size: 223 4323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 899 952 1234 1285 2369 2801 3550 3818
Size: 487 53 282 51 1084 432 749 268
Cuts at: 3818 412
Size: 1140
Fragments arranged by size:
1140 1084 749 487 432 282 268 53
51
EcoO109I rG'GnC_Cy
Cuts at: 517 1114 1683 517
Size: 597 569 3380
Fragments arranged by size:
3380 597 569
EcoRI G'AATT_C
Cuts at: 700 1548 700
Size: 848 3698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4546
FspI TGC'GCA
Cuts at: 2325 2325
Size: 4546
HaeII r_GCGC'y
Cuts at: 1978 2226 3250 4229 1978
Size: 248 1024 979 2295
Fragments arranged by size:
2295 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 799 831 2386 2416 2418 2448 253
Size: 32 514 32 1555 30 2 30 2351
Fragments arranged by size:
2351 1555 514 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1583 1583
Size: 4546
HindIII A'AGCT_T
Cuts at: 538 668 538
Size: 130 4416
KasI G'GCGC_C
Cuts at: 1974 2222 1974
Size: 248 4298
KpnI G_GTAC'C
Cuts at: 1564 1564
Size: 4546
MluI A'CGCG_T
Cuts at: 3979 3979
Size: 4546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 169 804 1022 3217 3401 169
Size: 635 218 2195 184 1314
Fragments arranged by size:
2195 1314 635 218 184
MscI TGG'CCA
Cuts at: 352 2305 352
Size: 1953 2593
MslI CAynn'nnrTG
Cuts at: 346 947 2533 2660 346
Size: 601 1586 127 2232
Fragments arranged by size:
2232 1586 601 127
MspA1I CmG'CkG
Cuts at: 1317 1655 1969 2329 3344 3589 1317
Size: 338 314 360 1015 245 2274
Fragments arranged by size:
2274 1015 360 338 314 245
NaeI GCC'GGC
Cuts at: 2725 2725
Size: 4546
NarI GG'CG_CC
Cuts at: 1975 2223 1975
Size: 248 4298
NcoI C'CATG_G
Cuts at: 191 227 191
Size: 36 4510
NgoMIV G'CCGG_C
Cuts at: 2723 2723
Size: 4546
NruI TCG'CGA
Cuts at: 2907 2907
Size: 4546
NsiI A_TGCA'T
Cuts at: 987 987
Size: 4546
NspI r_CATG'y
Cuts at: 1597 2097 3006 1597
Size: 500 909 3137
Fragments arranged by size:
3137 909 500
PciI A'CATG_T
Cuts at: 3002 3002
Size: 4546
PfoI T'CCnGG_A
Cuts at: 585 585
Size: 4546
PmeI GTTT'AAAC
Cuts at: 1543 1543
Size: 4546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3685 3717 3685
Size: 32 4514
PpuMI rG'GwC_Cy
Cuts at: 1114 1114
Size: 4546
PstI C_TGCA'G
Cuts at: 1137 1364 1591 2276 1137
Size: 227 227 685 3407
Fragments arranged by size:
3407 685 227 227
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4546
PvuII CAG'CTG
Cuts at: 1317 1969 2329 1317
Size: 652 360 3534
Fragments arranged by size:
3534 652 360
RsrII CG'GwC_CG
Cuts at: 2739 2739
Size: 4546
SacI G_AGCT'C
Cuts at: 1335 1558 1335
Size: 223 4323
SalI G'TCGA_C
Cuts at: 1581 1581
Size: 4546
SanDI GG'GwC_CC
Cuts at: 1114 1114
Size: 4546
SapI GCTCTTCn'nnn_
Cuts at: 609 2567 2777 609
Size: 1958 210 2378
Fragments arranged by size:
2378 1958 210
SbfI CC_TGCA'GG
Cuts at: 1591 1591
Size: 4546
ScaI AGT'ACT
Cuts at: 4063 4063
Size: 4546
SfcI C'TryA_G
Cuts at: 33 1133 1360 1587 2272 3267 3458 33
Size: 1100 227 227 685 995 191 1121
Fragments arranged by size:
1121 1100 995 685 227 227 191
SfoI GGC'GCC
Cuts at: 1976 2224 1976
Size: 248 4298
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4546
SmaI CCC'GGG
Cuts at: 1566 1566
Size: 4546
SmlI C'TyrA_G
Cuts at: 592 850 1336 1699 2614 3108 3370 592
Size: 258 486 363 915 494 262 1768
Fragments arranged by size:
1768 915 494 486 363 262 258
SnaBI TAC'GTA
Cuts at: 1536 1536
Size: 4546
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4546
SphI G_CATG'C
Cuts at: 1597 2097 1597
Size: 500 4046
StyI C'CwwG_G
Cuts at: 191 227 1678 2032 191
Size: 36 1451 354 2705
Fragments arranged by size:
2705 1451 354 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 2127 2260 2719 2930 163
Size: 1964 133 459 211 1779
Fragments arranged by size:
1964 1779 459 211 133
TatI w'GTAC_w
Cuts at: 4061 4061
Size: 4546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4063 4095 4063
Size: 32 4514
Tth111I GACn'n_nGTC
Cuts at: 2341 2341
Size: 4546
XbaI T'CTAG_A
Cuts at: 522 1575 522
Size: 1053 3493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2039 2039
Size: 4546
XmaI C'CCGG_G
Cuts at: 1564 1564
Size: 4546
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BsaXI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI