Restriction map for
pF1KSDA0848
(Circular) (Six-base) MAPSORT of: pF1KSDA0848.seq Check: 9391 from: 1 to: 6070
pF1KSDA0848 6070 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2295 3106 2295
Size: 811 5259
Acc65I G'GTAC_C
Cuts at: 3084 3084
Size: 6070
AclI AA'CG_TT
Cuts at: 5910 5910
Size: 6070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 874 1608 2172 3893 4325 5074 5342 528
Size: 346 734 564 1721 432 749 268 1256
Fragments arranged by size:
1721 1256 749 734 564 432 346 268
AflII C'TTAA_G
Cuts at: 496 496
Size: 6070
AflIII A'CryG_T
Cuts at: 835 3420 4526 5503 835
Size: 2585 1106 977 1402
Fragments arranged by size:
2585 1402 1106 977
AgeI A'CCGG_T
Cuts at: 4458 4458
Size: 6070
AleI CACnn'nnGTG
Cuts at: 2614 2614
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2248 2248
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 3373 4942 3373
Size: 1569 4501
ApaI G_GGCC'C
Cuts at: 2074 2583 2074
Size: 509 5561
ApaLI G'TGCA_C
Cuts at: 2869 4840 2869
Size: 1971 4099
ApoI r'AATT_y
Cuts at: 384 1007 1413 3072 5423 5679 384
Size: 623 406 1659 2351 256 775
Fragments arranged by size:
2351 1659 775 623 406 256
AseI AT'TA_AT
Cuts at: 20 443 20
Size: 423 5647
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6070
AvaI C'yCGr_G
Cuts at: 2986 3088 2986
Size: 102 5968
AvrII C'CTAG_G
Cuts at: 2499 2499
Size: 6070
BamHI G'GATC_C
Cuts at: 3093 3093
Size: 6070
BanI G'GyrC_C
Cuts at: 2028 3084 3498 3746 3781 2028
Size: 1056 414 248 35 4317
Fragments arranged by size:
4317 1056 414 248 35
BanII G_rGCy'C
Cuts at: 254 806 1906 2074 2583 3082 4112 254
Size: 552 1100 168 509 499 1030 2212
Fragments arranged by size:
2212 1100 1030 552 509 499 168
BbeI G_GCGC'C
Cuts at: 3502 3750 3502
Size: 248 5822
BbsI GAAGACnn'nnnn_
Cuts at: 534 961 3046 534
Size: 427 2085 3558
Fragments arranged by size:
3558 2085 427
BciVI GTATCCnnnnn_n'
Cuts at: 3966 4729 6070 3966
Size: 763 1341 3966
Fragments arranged by size:
3966 1341 763
BclI T'GATC_A
Cuts at: 3588 3588
Size: 6070
BfrBI ATG'CAT
Cuts at: 460 1039 460
Size: 579 5491
BglII A'GATC_T
Cuts at: 3583 5622 3583
Size: 2039 4031
BlpI GC'TnA_GC
Cuts at: 3180 3180
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 2031 2074 2583 2849 2873 3693 3786 4844 2031
Size: 43 509 266 24 820 93 1058 3257
Fragments arranged by size:
3257 1058 820 509 266 93 43 24
BmrI ACTGGGnnnn_n'
Cuts at: 1259 2693 3280 3361 3696 5842 1259
Size: 1434 587 81 335 2146 1487
Fragments arranged by size:
2146 1487 1434 587 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4435 4435
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 790 2006 2027 2297 3055 500
Size: 290 1216 21 270 758 3515
Fragments arranged by size:
3515 1216 758 290 270 21
Bpu10I CC'TnA_GC
Cuts at: 1124 1124
Size: 6070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3244 4123 4617 4915 3244
Size: 879 494 298 4399
Fragments arranged by size:
4399 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 904 5774 904
Size: 4870 1200
BsaAI yAC'GTr
Cuts at: 736 2157 2868 3060 3421 736
Size: 1421 711 192 361 3385
Fragments arranged by size:
3385 1421 711 361 192
BsaBI GATnn'nnATC
Cuts at: 6 3582 5621 6
Size: 3576 2039 455
Fragments arranged by size:
3576 2039 455
BsaHI Gr'CG_yC
Cuts at: 1691 2169 3499 3747 4451 5947 1691
Size: 478 1330 248 704 1496 1814
Fragments arranged by size:
1814 1496 1330 704 478 248
BsaWI w'CCGG_w
Cuts at: 2549 3257 3778 4458 4732 4879 5290 2549
Size: 708 521 680 274 147 411 3329
Fragments arranged by size:
3329 708 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2051 2419 2422 2425 2051
Size: 368 3 3 5696
Fragments arranged by size:
5696 368 3 3
BseYI C'CCAG_C
Cuts at: 1995 2000 2600 2646 3493 4251 4830 5326 1995
Size: 5 600 46 847 758 579 496 2739
Fragments arranged by size:
2739 847 758 600 579 496 46 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1806 1954 2468 2793 2848 1806
Size: 148 514 325 55 5028
Fragments arranged by size:
5028 514 325 148 55
BsiEI CG_ry'CG
Cuts at: 104 3610 3656 4866 104
Size: 3506 46 1210 1308
Fragments arranged by size:
3506 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 806 2162 2873 3082 3860 4050 4844 806
Size: 1356 711 209 778 190 794 2032
Fragments arranged by size:
2032 1356 794 778 711 209 190
BsmI GAATG_Cn'
Cuts at: 776 872 1041 1356 1785 776
Size: 96 169 315 429 5061
Fragments arranged by size:
5061 429 315 169 96
Bsp1286I G_dGCh'C
Cuts at: 254 806 1906 2031 2074 2162 2583 2849 2873
Size: 552 1100 125 43 88 421 266 24
Cuts at: 2873 3082 3693 3786 3860 4050 4112 4844 254
Size: 209 611 93 74 190 62 732 1480
Fragments arranged by size:
1480 1100 732 611 552 421 266 209
190 125 93 88 74 62 43 24
BspEI T'CCGG_A
Cuts at: 5290 5290
Size: 6070
BspHI T'CATG_A
Cuts at: 5246 5246
Size: 6070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3118 3332 3634 4015 3118
Size: 214 302 381 5173
Fragments arranged by size:
5173 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4360 4414 5900 6065 50
Size: 4310 54 1486 165 55
Fragments arranged by size:
4310 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 460 1202 2242 3980 460
Size: 742 1040 1738 2550
Fragments arranged by size:
2550 1738 1040 742
BsrFI r'CCGG_y
Cuts at: 4066 4247 4458 4066
Size: 181 211 5678
Fragments arranged by size:
5678 211 181
BsrGI T'GTAC_A
Cuts at: 832 846 832
Size: 14 6056
BssSI C'ACGA_G
Cuts at: 2399 4339 4699 2399
Size: 1940 360 3770
Fragments arranged by size:
3770 1940 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2446 3445 2446
Size: 999 5071
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6070
BstEII G'GTnAC_C
Cuts at: 2364 2364
Size: 6070
BstXI CCAn_nnnn'nTGG
Cuts at: 928 928
Size: 6070
BstYI r'GATC_y
Cuts at: 1 1428 3093 3560 3583 3918 4164 5178 5264
Size: 1427 1665 467 23 335 246 1014 86
Cuts at: 5264 5276 5622 1
Size: 12 346 449
Fragments arranged by size:
1665 1427 1014 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1726 1726
Size: 6070
BtgI C'CryG_G
Cuts at: 927 1754 2330 927
Size: 827 576 4667
Fragments arranged by size:
4667 827 576
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4018 4199 4362 4018
Size: 181 163 5726
Fragments arranged by size:
5726 181 163
BtsI GCAGTG_nn'
Cuts at: 2594 3311 2594
Size: 717 5353
DraI TTT'AAA
Cuts at: 366 506 665 1845 3067 366
Size: 140 159 1180 1222 3369
Fragments arranged by size:
3369 1222 1180 159 140
DraIII CAC_nnn'GTG
Cuts at: 1253 1253
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 3774 4634 3774
Size: 860 5210
EaeI y'GGCC_r
Cuts at: 1042 3653 3827 4218 4245 5592 1042
Size: 2611 174 391 27 1347 1520
Fragments arranged by size:
2611 1520 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3653 3653
Size: 6070
EarI CTCTTCn'nnn_
Cuts at: 850 1513 2938 4091 4301 850
Size: 663 1425 1153 210 2619
Fragments arranged by size:
2619 1425 1153 663 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2291 2695 4276 4588 4734 5880 2291
Size: 404 1581 312 146 1146 2481
Fragments arranged by size:
2481 1581 1146 404 312 146
EcoICRI GAG'CTC
Cuts at: 804 3080 804
Size: 2276 3794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 528 790 874 1608 2006 2027 2172 2297
Size: 28 262 84 734 398 21 145 125
Cuts at: 2297 3055 3893 4325 5074 5342 500
Size: 758 838 432 749 268 1228
Fragments arranged by size:
1228 838 758 749 734 432 398 268
262 145 125 84 28 21
EcoNI CCTnn'n_nnAGG
Cuts at: 226 2750 226
Size: 2524 3546
EcoO109I rG'GnC_Cy
Cuts at: 936 1343 1475 2070 2071 2322 2580 3207 936
Size: 407 132 595 1 251 258 627 3799
Fragments arranged by size:
3799 627 595 407 258 251 132 1
EcoRI G'AATT_C
Cuts at: 384 1007 3072 384
Size: 623 2065 3382
Fragments arranged by size:
3382 2065 623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1524 1755 2202 2944 4087 1524
Size: 231 447 742 1143 3507
Fragments arranged by size:
3507 1143 742 447 231
FspI TGC'GCA
Cuts at: 1933 3849 1933
Size: 1916 4154
FspAI rTGC'GCAy
Cuts at: 1933 1933
Size: 6070
HaeII r_GCGC'y
Cuts at: 3502 3750 4774 5753 3502
Size: 248 1024 979 3819
Fragments arranged by size:
3819 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 337 369 501 533 1214 1246 1362 1394 2610
Size: 32 132 32 681 32 116 32 1216
Cuts at: 2610 2642 3910 3940 3942 3972 337
Size: 32 1268 30 2 30 2435
Fragments arranged by size:
2435 1268 1216 681 132 116 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1296 2296 3107 1296
Size: 1000 811 4259
Fragments arranged by size:
4259 1000 811
HindIII A'AGCT_T
Cuts at: 1172 1172
Size: 6070
KasI G'GCGC_C
Cuts at: 3498 3746 3498
Size: 248 5822
KpnI G_GTAC'C
Cuts at: 3088 3088
Size: 6070
MfeI C'AATT_G
Cuts at: 191 191
Size: 6070
MluI A'CGCG_T
Cuts at: 5503 5503
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 715 4741 4925 715
Size: 4026 184 1860
Fragments arranged by size:
4026 1860 184
MscI TGG'CCA
Cuts at: 1044 3829 1044
Size: 2785 3285
MslI CAynn'nnrTG
Cuts at: 682 926 1274 1778 1925 2230 2342 2614 4057
Size: 244 348 504 147 305 112 272 1443
Cuts at: 4057 4184 682
Size: 127 2568
Fragments arranged by size:
2568 1443 504 348 305 272 244 147
127 112
MspA1I CmG'CkG
Cuts at: 810 1984 3179 3493 3853 4868 5113 810
Size: 1174 1195 314 360 1015 245 1767
Fragments arranged by size:
1767 1195 1174 1015 360 314 245
NaeI GCC'GGC
Cuts at: 4249 4249
Size: 6070
NarI GG'CG_CC
Cuts at: 3499 3747 3499
Size: 248 5822
NcoI C'CATG_G
Cuts at: 927 927
Size: 6070
NgoMIV G'CCGG_C
Cuts at: 4247 4247
Size: 6070
NruI TCG'CGA
Cuts at: 4431 4431
Size: 6070
NsiI A_TGCA'T
Cuts at: 462 1041 462
Size: 579 5491
NspI r_CATG'y
Cuts at: 839 1551 2886 3121 3621 4530 839
Size: 712 1335 235 500 909 2379
Fragments arranged by size:
2379 1335 909 712 500 235
PciI A'CATG_T
Cuts at: 835 4526 835
Size: 3691 2379
PflMI CCAn_nnn'nTGG
Cuts at: 2653 2653
Size: 6070
PfoI T'CCnGG_A
Cuts at: 1601 1766 3032 1601
Size: 165 1266 4639
Fragments arranged by size:
4639 1266 165
PmeI GTTT'AAAC
Cuts at: 3067 3067
Size: 6070
PmlI CAC'GTG
Cuts at: 2868 2868
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5209 5241 5209
Size: 32 6038
PpuMI rG'GwC_Cy
Cuts at: 936 936
Size: 6070
PspOMI G'GGCC_C
Cuts at: 2070 2579 2070
Size: 509 5561
PspXI vC'TCGA_Gb
Cuts at: 2986 2986
Size: 6070
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1573 1605 1573
Size: 32 6038
PstI C_TGCA'G
Cuts at: 380 590 1620 2142 3115 3800 380
Size: 210 1030 522 973 685 2650
Fragments arranged by size:
2650 1030 973 685 522 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6070
PvuII CAG'CTG
Cuts at: 810 1984 3493 3853 810
Size: 1174 1509 360 3027
Fragments arranged by size:
3027 1509 1174 360
RsrII CG'GwC_CG
Cuts at: 4263 4263
Size: 6070
SacI G_AGCT'C
Cuts at: 806 3082 806
Size: 2276 3794
SalI G'TCGA_C
Cuts at: 2294 3105 2294
Size: 811 5259
SapI GCTCTTCn'nnn_
Cuts at: 4091 4301 4091
Size: 210 5860
SbfI CC_TGCA'GG
Cuts at: 3115 3115
Size: 6070
ScaI AGT'ACT
Cuts at: 5587 5587
Size: 6070
SexAI A'CCwGG_T
Cuts at: 1832 1832
Size: 6070
SfcI C'TryA_G
Cuts at: 33 376 586 1561 1616 2138 3111 3796 4791
Size: 343 210 975 55 522 973 685 995
Cuts at: 4791 4982 33
Size: 191 1121
Fragments arranged by size:
1121 995 975 973 685 522 343 210
191 55
SfoI GGC'GCC
Cuts at: 3500 3748 3500
Size: 248 5822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6070
SmaI CCC'GGG
Cuts at: 3090 3090
Size: 6070
SmlI C'TyrA_G
Cuts at: 496 2986 3223 4138 4632 4894 496
Size: 2490 237 915 494 262 1672
Fragments arranged by size:
2490 1672 915 494 262 237
SnaBI TAC'GTA
Cuts at: 3060 3060
Size: 6070
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6070
SphI G_CATG'C
Cuts at: 2886 3121 3621 2886
Size: 235 500 5335
Fragments arranged by size:
5335 500 235
SspI AAT'ATT
Cuts at: 330 330
Size: 6070
StuI AGG'CCT
Cuts at: 1559 2144 1559
Size: 585 5485
StyI C'CwwG_G
Cuts at: 561 823 927 1150 1281 1339 1424 1813 2499
Size: 262 104 223 131 58 85 389 686
Cuts at: 2499 2528 3202 3556 561
Size: 29 674 354 3075
Fragments arranged by size:
3075 686 674 389 354 262 223 131
104 85 58 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1241 2476 2541 2660 3651 3784 4243 4454 1241
Size: 1235 65 119 991 133 459 211 2857
Fragments arranged by size:
2857 1235 991 459 211 133 119 65
TatI w'GTAC_w
Cuts at: 832 846 1580 2348 5585 832
Size: 14 734 768 3237 1317
Fragments arranged by size:
3237 1317 768 734 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5587 5619 5587
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1834 3865 1834
Size: 2031 4039
XbaI T'CTAG_A
Cuts at: 576 3099 576
Size: 2523 3547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2650 2719 3563 2650
Size: 69 844 5157
Fragments arranged by size:
5157 844 69
XhoI C'TCGA_G
Cuts at: 2986 2986
Size: 6070
XmaI C'CCGG_G
Cuts at: 3088 3088
Size: 6070
XmnI GAAnn'nnTTC
Cuts at: 872 1334 1738 2927 872
Size: 462 404 1189 4015
Fragments arranged by size:
4015 1189 462 404
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI AscI BaeI BbvCI
BcgI BglI BmgBI BmtI BsaXI BsiWI BsmBI BssHII
BstZ17I ClaI CspCI EcoRV FseI HpaI NdeI NheI
NotI PacI PasI PshAI PsiI SacII SanDI SfiI
SgrAI SrfI SwaI ZraI