Restriction map for
pF1KSDA0845
(Circular) (Six-base) MAPSORT of: pF1KSDA0845.seq Check: 8411 from: 1 to: 6217
pF1KSDA0845 6217 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 653 653
Size: 6217
AatII G_ACGT'C
Cuts at: 286 931 286
Size: 645 5572
AccI GT'mk_AC
Cuts at: 3253 3253
Size: 6217
Acc65I G'GTAC_C
Cuts at: 3231 3231
Size: 6217
AclI AA'CG_TT
Cuts at: 6057 6057
Size: 6217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1547 1559 2572 2855 4040 4472 5221 5489
Size: 613 12 1013 283 1185 432 749 268
Cuts at: 5489 934
Size: 1662
Fragments arranged by size:
1662 1185 1013 749 613 432 283 268
12
AfeI AGC'GCT
Cuts at: 1162 1162
Size: 6217
AflIII A'CryG_T
Cuts at: 3567 4673 5650 3567
Size: 1106 977 4134
Fragments arranged by size:
4134 1106 977
AgeI A'CCGG_T
Cuts at: 4605 4605
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 927 1023 927
Size: 96 6121
AlwNI CAG_nnn'CTG
Cuts at: 435 792 1224 1287 3520 5089 435
Size: 357 432 63 2233 1569 1563
Fragments arranged by size:
2233 1569 1563 432 357 63
ApaLI G'TGCA_C
Cuts at: 4987 4987
Size: 6217
ApoI r'AATT_y
Cuts at: 1409 2482 3219 5570 5826 1409
Size: 1073 737 2351 256 1800
Fragments arranged by size:
2351 1800 1073 737 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6217
AvaI C'yCGr_G
Cuts at: 649 1867 2341 3235 649
Size: 1218 474 894 3631
Fragments arranged by size:
3631 1218 894 474
BamHI G'GATC_C
Cuts at: 3240 3240
Size: 6217
BanI G'GyrC_C
Cuts at: 160 331 818 871 2733 3231 3645 3893 3928
Size: 171 487 53 1862 498 414 248 35
Cuts at: 3928 160
Size: 2449
Fragments arranged by size:
2449 1862 498 487 414 248 171 53
35
BanII G_rGCy'C
Cuts at: 295 868 2657 3229 4259 295
Size: 573 1789 572 1030 2253
Fragments arranged by size:
2253 1789 1030 573 572
BbeI G_GCGC'C
Cuts at: 164 335 822 875 3649 3897 164
Size: 171 487 53 2774 248 2484
Fragments arranged by size:
2774 2484 487 248 171 53
BbsI GAAGACnn'nnnn_
Cuts at: 2903 3188 2903
Size: 285 5932
BbvCI CC'TCA_GC
Cuts at: 1237 1709 1853 1913 1973 2015 1237
Size: 472 144 60 60 42 5439
Fragments arranged by size:
5439 472 144 60 60 42
BciVI GTATCCnnnnn_n'
Cuts at: 4113 4876 6217 4113
Size: 763 1341 4113
Fragments arranged by size:
4113 1341 763
BclI T'GATC_A
Cuts at: 3735 3735
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 503 715 757 880 503
Size: 212 42 123 5840
Fragments arranged by size:
5840 212 123 42
BglII A'GATC_T
Cuts at: 3730 5769 3730
Size: 2039 4178
BlpI GC'TnA_GC
Cuts at: 364 1062 1232 3327 364
Size: 698 170 2095 3254
Fragments arranged by size:
3254 2095 698 170
Bme1580I G_kGCm'C
Cuts at: 3840 3933 4991 3840
Size: 93 1058 5066
Fragments arranged by size:
5066 1058 93
BmgBI CAC'GTC
Cuts at: 924 924
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 1939 1981 2143 2185 2227 2269 2311 2353 2605
Size: 42 162 42 42 42 42 42 252
Cuts at: 2605 3427 3508 3843 5989 1939
Size: 822 81 335 2146 2167
Fragments arranged by size:
2167 2146 822 335 252 162 81 42
42 42 42 42 42
BmtI G_CTAG'C
Cuts at: 211 211
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4582 4582
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 499 520 652 1141 1171 1192 1766 1832
Size: 259 21 132 489 30 21 574 66
Cuts at: 1832 1910 1994 2417 2432 3120 3149 240
Size: 78 84 423 15 688 29 3308
Fragments arranged by size:
3308 688 574 489 423 259 132 84
78 66 30 29 21 21 15
Bpu10I CC'TnA_GC
Cuts at: 1237 1709 1853 1913 1973 2015 1237
Size: 472 144 60 60 42 5439
Fragments arranged by size:
5439 472 144 60 60 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 3391 4270 4764 5062 324
Size: 3067 879 494 298 1479
Fragments arranged by size:
3067 1479 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1507 5921 1507
Size: 4414 1803
BsaAI yAC'GTr
Cuts at: 666 3207 3568 666
Size: 2541 361 3315
Fragments arranged by size:
3315 2541 361
BsaBI GATnn'nnATC
Cuts at: 6 3729 5768 6
Size: 3723 2039 455
Fragments arranged by size:
3723 2039 455
BsaHI Gr'CG_yC
Cuts at: 161 283 332 819 872 908 928 1092 3646
Size: 122 49 487 53 36 20 164 2554
Cuts at: 3646 3894 4598 6094 161
Size: 248 704 1496 284
Fragments arranged by size:
2554 1496 704 487 284 248 164 122
53 49 36 20
BsaWI w'CCGG_w
Cuts at: 2997 3404 3925 4605 4879 5026 5437 2997
Size: 407 521 680 274 147 411 3777
Fragments arranged by size:
3777 680 521 411 407 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1183 1213 1617 1647 2509 2539 1183
Size: 30 404 30 862 30 4861
Fragments arranged by size:
4861 862 404 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 243 718 814 1009 1087 1525 1561 1579 1582
Size: 475 96 195 78 438 36 18 3
Cuts at: 1582 1615 2530 2635 2638 2719 2743 2782 3034
Size: 33 915 105 3 81 24 39 252
Cuts at: 3034 3055 243
Size: 21 3405
Fragments arranged by size:
3405 915 475 438 252 195 105 96
81 78 39 36 33 24 21 18
3 3
BseYI C'CCAG_C
Cuts at: 156 310 1265 1638 1746 1884 2965 3640 4398
Size: 154 955 373 108 138 1081 675 758
Cuts at: 4398 4977 5473 156
Size: 579 496 900
Fragments arranged by size:
1081 955 900 758 675 579 496 373
154 138 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 994
Size: 6217
BsiEI CG_ry'CG
Cuts at: 104 444 3757 3803 5013 104
Size: 340 3313 46 1210 1308
Fragments arranged by size:
3313 1308 1210 340 46
BsiHKAI G_wGCw'C
Cuts at: 295 644 982 3229 4007 4197 4991 295
Size: 349 338 2247 778 190 794 1521
Fragments arranged by size:
2247 1521 794 778 349 338 190
BsmBI CGTCTCn'nnnn_
Cuts at: 892 892
Size: 6217
Bsp1286I G_dGCh'C
Cuts at: 295 644 868 982 2657 3229 3840 3933 4007
Size: 349 224 114 1675 572 611 93 74
Cuts at: 4007 4197 4259 4991 295
Size: 190 62 732 1521
Fragments arranged by size:
1675 1521 732 611 572 349 224 190
114 93 74 62
BspEI T'CCGG_A
Cuts at: 5437 5437
Size: 6217
BspHI T'CATG_A
Cuts at: 5393 5393
Size: 6217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 653 779 821 1295 2790 3265 3479 3781 4162
Size: 126 42 474 1495 475 214 302 381
Cuts at: 4162 653
Size: 2708
Fragments arranged by size:
2708 1495 475 474 381 302 214 126
42
BsrBI CCG'CTC
Cuts at: 50 542 4507 4561 6047 6212 50
Size: 492 3965 54 1486 165 55
Fragments arranged by size:
3965 1486 492 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1193 4127 1193
Size: 2934 3283
BsrFI r'CCGG_y
Cuts at: 1088 1704 1908 1968 4213 4394 4605 1088
Size: 616 204 60 2245 181 211 2700
Fragments arranged by size:
2700 2245 616 211 204 181 60
BssHII G'CGCG_C
Cuts at: 613 725 733 760 950 613
Size: 112 8 27 190 5880
Fragments arranged by size:
5880 190 112 27 8
BssSI C'ACGA_G
Cuts at: 270 4486 4846 270
Size: 4216 360 1641
Fragments arranged by size:
4216 1641 360
BstAPI GCAn_nnn'nTGC
Cuts at: 792 3592 792
Size: 2800 3417
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 1119 1119
Size: 6217
BstYI r'GATC_y
Cuts at: 1 857 2582 3240 3707 3730 4065 4311 5325
Size: 856 1725 658 467 23 335 246 1014
Cuts at: 5325 5411 5423 5769 1
Size: 86 12 346 449
Fragments arranged by size:
1725 1014 856 658 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1811 3038 3110 1811
Size: 1227 72 4918
Fragments arranged by size:
4918 1227 72
BtgI C'CryG_G
Cuts at: 321 321
Size: 6217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 642 4165 4346 4509 642
Size: 3523 181 163 2350
Fragments arranged by size:
3523 2350 181 163
BtsI GCAGTG_nn'
Cuts at: 417 3458 417
Size: 3041 3176
DraI TTT'AAA
Cuts at: 3214 3214
Size: 6217
DraIII CAC_nnn'GTG
Cuts at: 412 972 1250 412
Size: 560 278 5379
Fragments arranged by size:
5379 560 278
DrdI GACnn_nn'nnGTC
Cuts at: 282 3921 4781 282
Size: 3639 860 1718
Fragments arranged by size:
3639 1718 860
EaeI y'GGCC_r
Cuts at: 398 852 1259 2927 3800 3974 4365 4392 5739
Size: 454 407 1668 873 174 391 27 1347
Cuts at: 5739 398
Size: 876
Fragments arranged by size:
1668 1347 876 873 454 407 391 174
27
EagI C'GGCC_G
Cuts at: 3800 3800
Size: 6217
EarI CTCTTCn'nnn_
Cuts at: 827 1346 1442 1538 1592 1640 1685 1787 1889
Size: 519 96 96 54 48 45 102 102
Cuts at: 1889 2111 2591 2669 2822 4238 4448 827
Size: 222 480 78 153 1416 210 2596
Fragments arranged by size:
2596 1416 519 480 222 210 153 102
102 96 96 78 54 48 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 231 291 4423 4735 4881 6027 231
Size: 60 4132 312 146 1146 421
Fragments arranged by size:
4132 1146 421 312 146 60
EcoICRI GAG'CTC
Cuts at: 293 3227 293
Size: 2934 3283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 499 520 652 934 1141 1171 1192 1547
Size: 259 21 132 282 207 30 21 355
Cuts at: 1547 1559 1766 1832 1910 1994 2417 2432 2572
Size: 12 207 66 78 84 423 15 140
Cuts at: 2572 2855 3120 3149 4040 4472 5221 5489 240
Size: 283 265 29 891 432 749 268 968
Fragments arranged by size:
968 891 749 432 423 355 283 282
268 265 259 207 207 140 132 84
78 66 30 29 21 21 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 634 649 2554 634
Size: 15 1905 4297
Fragments arranged by size:
4297 1905 15
EcoO109I rG'GnC_Cy
Cuts at: 1284 2468 2568 3354 1284
Size: 1184 100 786 4147
Fragments arranged by size:
4147 1184 786 100
EcoRI G'AATT_C
Cuts at: 3219 3219
Size: 6217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4234 4234
Size: 6217
FspI TGC'GCA
Cuts at: 3996 3996
Size: 6217
HaeII r_GCGC'y
Cuts at: 164 335 436 520 678 793 822 875 937
Size: 171 101 84 158 115 29 53 62
Cuts at: 937 1164 3649 3897 4921 5900 164
Size: 227 2485 248 1024 979 481
Fragments arranged by size:
2485 1024 979 481 248 227 171 158
115 101 84 62 53 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 913 945 1009 1041 1183 1215 1639 1671 1684
Size: 32 64 32 142 32 424 32 13
Cuts at: 1684 1702 1716 1734 1786 1804 1818 1836 1846
Size: 18 14 18 52 18 14 18 10
Cuts at: 1846 1878 1888 1906 1920 1938 1966 1998 2008
Size: 32 10 18 14 18 28 32 10
Cuts at: 2008 2040 2110 2142 2413 2445 2509 2541 2590
Size: 32 70 32 271 32 64 32 49
Cuts at: 2590 2622 4057 4087 4089 4119 913
Size: 32 1435 30 2 30 3011
Fragments arranged by size:
3011 1435 424 271 142 70 64 64
52 49 32 32 32 32 32 32
32 32 32 32 32 30 30 28
18 18 18 18 18 18 14 14
14 13 10 10 10 2
HincII GTy'rAC
Cuts at: 3254 3254
Size: 6217
KasI G'GCGC_C
Cuts at: 160 331 818 871 3645 3893 160
Size: 171 487 53 2774 248 2484
Fragments arranged by size:
2774 2484 487 248 171 53
KpnI G_GTAC'C
Cuts at: 3235 3235
Size: 6217
MluI A'CGCG_T
Cuts at: 5650 5650
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1007 1339 2071 4888 5072 1007
Size: 332 732 2817 184 2152
Fragments arranged by size:
2817 2152 732 332 184
MscI TGG'CCA
Cuts at: 400 2929 3976 400
Size: 2529 1047 2641
Fragments arranged by size:
2641 2529 1047
MslI CAynn'nnrTG
Cuts at: 1435 4204 4331 1435
Size: 2769 127 3321
Fragments arranged by size:
3321 2769 127
MspA1I CmG'CkG
Cuts at: 177 247 260 426 477 1098 1224 1269 1852
Size: 70 13 166 51 621 126 45 583
Cuts at: 1852 2014 3326 3640 4000 5015 5260 177
Size: 162 1312 314 360 1015 245 1134
Fragments arranged by size:
1312 1134 1015 621 583 360 314 245
166 162 126 70 51 45 13
NaeI GCC'GGC
Cuts at: 4396 4396
Size: 6217
NarI GG'CG_CC
Cuts at: 161 332 819 872 3646 3894 161
Size: 171 487 53 2774 248 2484
Fragments arranged by size:
2774 2484 487 248 171 53
NgoMIV G'CCGG_C
Cuts at: 4394 4394
Size: 6217
NheI G'CTAG_C
Cuts at: 207 207
Size: 6217
NruI TCG'CGA
Cuts at: 4578 4578
Size: 6217
NspI r_CATG'y
Cuts at: 3268 3768 4677 3268
Size: 500 909 4808
Fragments arranged by size:
4808 909 500
PciI A'CATG_T
Cuts at: 4673 4673
Size: 6217
PfoI T'CCnGG_A
Cuts at: 1340 1340
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3214 3214
Size: 6217
PmlI CAC'GTG
Cuts at: 666 666
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 985 1017 5356 5388 985
Size: 32 4339 32 1814
Fragments arranged by size:
4339 1814 32 32
PpuMI rG'GwC_Cy
Cuts at: 1284 2468 1284
Size: 1184 5033
PspXI vC'TCGA_Gb
Cuts at: 649 649
Size: 6217
PstI C_TGCA'G
Cuts at: 431 776 797 989 1103 2478 3262 3947 431
Size: 345 21 192 114 1375 784 685 2701
Fragments arranged by size:
2701 1375 784 685 345 192 114 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6217
PvuII CAG'CTG
Cuts at: 426 477 1098 1224 1269 1852 2014 3640 4000
Size: 51 621 126 45 583 162 1626 360
Cuts at: 4000 426
Size: 2643
Fragments arranged by size:
2643 1626 621 583 360 162 126 51
45
RsrII CG'GwC_CG
Cuts at: 4410 4410
Size: 6217
SacI G_AGCT'C
Cuts at: 295 3229 295
Size: 2934 3283
SalI G'TCGA_C
Cuts at: 3252 3252
Size: 6217
SapI GCTCTTCn'nnn_
Cuts at: 4238 4448 4238
Size: 210 6007
SbfI CC_TGCA'GG
Cuts at: 3262 3262
Size: 6217
ScaI AGT'ACT
Cuts at: 5734 5734
Size: 6217
SfcI C'TryA_G
Cuts at: 33 427 772 793 985 1099 2474 3258 3943
Size: 394 345 21 192 114 1375 784 685
Cuts at: 3943 4938 5129 33
Size: 995 191 1121
Fragments arranged by size:
1375 1121 995 784 685 394 345 192
191 114 21
SfoI GGC'GCC
Cuts at: 162 333 820 873 3647 3895 162
Size: 171 487 53 2774 248 2484
Fragments arranged by size:
2774 2484 487 248 171 53
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6217
SmaI CCC'GGG
Cuts at: 3237 3237
Size: 6217
SmlI C'TyrA_G
Cuts at: 339 649 3370 4285 4779 5041 339
Size: 310 2721 915 494 262 1515
Fragments arranged by size:
2721 1515 915 494 310 262
SnaBI TAC'GTA
Cuts at: 3207 3207
Size: 6217
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6217
SphI G_CATG'C
Cuts at: 3268 3768 3268
Size: 500 5717
StyI C'CwwG_G
Cuts at: 1037 1139 2564 2759 2879 2951 2957 3014 3068
Size: 102 1425 195 120 72 6 57 54
Cuts at: 3068 3194 3349 3703 1037
Size: 126 155 354 3551
Fragments arranged by size:
3551 1425 354 195 155 126 120 102
72 57 54 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1034 2751 3798 3931 4390 4601 1034
Size: 1717 1047 133 459 211 2650
Fragments arranged by size:
2650 1717 1047 459 211 133
TatI w'GTAC_w
Cuts at: 5732 5732
Size: 6217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5734 5766 5734
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 4012 4012
Size: 6217
XbaI T'CTAG_A
Cuts at: 3246 3246
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1146 1221 1254 3710 1146
Size: 75 33 2456 3653
Fragments arranged by size:
3653 2456 75 33
XhoI C'TCGA_G
Cuts at: 649 649
Size: 6217
XmaI C'CCGG_G
Cuts at: 3235 3235
Size: 6217
XmnI GAAnn'nnTTC
Cuts at: 1214 1214
Size: 6217
ZraI GAC'GTC
Cuts at: 284 929 284
Size: 645 5572
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NruI NspI PciI PfoI
PmeI PmlI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AleI AlfI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BsiWI BsmI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NsiI PacI PasI PflMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI