Restriction map for
pF1KSDA0843
(Circular) (Six-base) MAPSORT of: pF1KSDA0843.seq Check: 1793 from: 1 to: 5188
pF1KSDA0843 5188 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 220 220
Size: 5188
AccI GT'mk_AC
Cuts at: 1486 2045 2224 1486
Size: 559 179 4450
Fragments arranged by size:
4450 559 179
Acc65I G'GTAC_C
Cuts at: 2202 2202
Size: 5188
AclI AA'CG_TT
Cuts at: 5028 5028
Size: 5188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 1726 2173 3011 3443 4192 4460 477
Size: 1249 447 838 432 749 268 1205
Fragments arranged by size:
1249 1205 838 749 447 432 268
AflIII A'CryG_T
Cuts at: 1130 2538 3644 4621 1130
Size: 1408 1106 977 1697
Fragments arranged by size:
1697 1408 1106 977
AgeI A'CCGG_T
Cuts at: 3576 3576
Size: 5188
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1170 1202 1170
Size: 32 5156
AlwNI CAG_nnn'CTG
Cuts at: 2491 4060 2491
Size: 1569 3619
ApaI G_GGCC'C
Cuts at: 1334 1334
Size: 5188
ApaLI G'TGCA_C
Cuts at: 235 3958 235
Size: 3723 1465
ApoI r'AATT_y
Cuts at: 2190 4541 4797 2190
Size: 2351 256 2581
Fragments arranged by size:
2581 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5188
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5188
AvaI C'yCGr_G
Cuts at: 920 1245 1333 1616 2206 920
Size: 325 88 283 590 3902
Fragments arranged by size:
3902 590 325 283 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 762 795 762
Size: 33 5155
BamHI G'GATC_C
Cuts at: 979 2211 979
Size: 1232 3956
BanI G'GyrC_C
Cuts at: 361 896 2202 2616 2864 2899 361
Size: 535 1306 414 248 35 2650
Fragments arranged by size:
2650 1306 535 414 248 35
BanII G_rGCy'C
Cuts at: 1334 1351 1735 1768 2200 3230 1334
Size: 17 384 33 432 1030 3292
Fragments arranged by size:
3292 1030 432 384 33 17
BbeI G_GCGC'C
Cuts at: 2620 2868 2620
Size: 248 4940
BbsI GAAGACnn'nnnn_
Cuts at: 1520 1520
Size: 5188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 715 749 992 1026 715
Size: 34 243 34 4877
Fragments arranged by size:
4877 243 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3084 3847 5188 3084
Size: 763 1341 3084
Fragments arranged by size:
3084 1341 763
BclI T'GATC_A
Cuts at: 2706 2706
Size: 5188
BglI GCCn_nnn'nGGC
Cuts at: 1280 1280
Size: 5188
BglII A'GATC_T
Cuts at: 1982 2701 4740 1982
Size: 719 2039 2430
Fragments arranged by size:
2430 2039 719
BlpI GC'TnA_GC
Cuts at: 2298 2298
Size: 5188
Bme1580I G_kGCm'C
Cuts at: 239 1334 1616 2811 2904 3962 239
Size: 1095 282 1195 93 1058 1465
Fragments arranged by size:
1465 1195 1095 1058 282 93
BmgBI CAC'GTC
Cuts at: 642 642
Size: 5188
BmrI ACTGGGnnnn_n'
Cuts at: 291 522 732 1971 2398 2479 2814 4960 291
Size: 231 210 1239 427 81 335 2146 519
Fragments arranged by size:
2146 1239 519 427 335 231 210 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3553 3553
Size: 5188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 1224 1451 1658 1168
Size: 56 227 207 4698
Fragments arranged by size:
4698 227 207 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2362 3241 3735 4033 2362
Size: 879 494 298 3517
Fragments arranged by size:
3517 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4892 4892
Size: 5188
BsaAI yAC'GTr
Cuts at: 2178 2539 2178
Size: 361 4827
BsaBI GATnn'nnATC
Cuts at: 6 2700 4739 6
Size: 2694 2039 455
Fragments arranged by size:
2694 2039 455
BsaHI Gr'CG_yC
Cuts at: 2617 2865 3569 5065 2617
Size: 248 704 1496 2740
Fragments arranged by size:
2740 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1448 2375 2896 3576 3850 3997 4408 1448
Size: 927 521 680 274 147 411 2228
Fragments arranged by size:
2228 927 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 470 500 1170 1200 1243 1273 1567 1597 470
Size: 30 670 30 43 30 294 30 4061
Fragments arranged by size:
4061 670 294 43 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 1655 1660 607
Size: 1048 5 4135
Fragments arranged by size:
4135 1048 5
BseYI C'CCAG_C
Cuts at: 1173 1425 1918 2611 3369 3948 4444 1173
Size: 252 493 693 758 579 496 1917
Fragments arranged by size:
1917 758 693 579 496 493 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 468 477 1774 1891 318
Size: 150 9 1297 117 3615
Fragments arranged by size:
3615 1297 150 117 9
BsiEI CG_ry'CG
Cuts at: 104 1729 1834 2728 2774 3984 104
Size: 1625 105 894 46 1210 1308
Fragments arranged by size:
1625 1308 1210 894 105 46
BsiHKAI G_wGCw'C
Cuts at: 239 526 1351 1735 1768 2200 2978 3168 3962
Size: 287 825 384 33 432 778 190 794
Cuts at: 3962 239
Size: 1465
Fragments arranged by size:
1465 825 794 778 432 384 287 190
33
BsmI GAATG_Cn'
Cuts at: 137 137
Size: 5188
BsmBI CGTCTCn'nnnn_
Cuts at: 1224 1224
Size: 5188
Bsp1286I G_dGCh'C
Cuts at: 239 526 1334 1351 1616 1735 1768 2200 2811
Size: 287 808 17 265 119 33 432 611
Cuts at: 2811 2904 2978 3168 3230 3962 239
Size: 93 74 190 62 732 1465
Fragments arranged by size:
1465 808 732 611 432 287 265 190
119 93 74 62 33 17
BspEI T'CCGG_A
Cuts at: 4408 4408
Size: 5188
BspHI T'CATG_A
Cuts at: 4364 4364
Size: 5188
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 673 2236 2450 2752 3133 220
Size: 453 1563 214 302 381 2275
Fragments arranged by size:
2275 1563 453 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1304 1325 3478 3532 5018 5183 50
Size: 1254 21 2153 54 1486 165 55
Fragments arranged by size:
2153 1486 1254 165 55 54 21
BsrDI GCAATG_nn'
Cuts at: 3098 3098
Size: 5188
BsrFI r'CCGG_y
Cuts at: 1274 1694 2123 3184 3365 3576 1274
Size: 420 429 1061 181 211 2886
Fragments arranged by size:
2886 1061 429 420 211 181
BssSI C'ACGA_G
Cuts at: 3457 3817 3457
Size: 360 4828
BstAPI GCAn_nnn'nTGC
Cuts at: 455 2563 455
Size: 2108 3080
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5188
BstEII G'GTnAC_C
Cuts at: 486 992 486
Size: 506 4682
BstXI CCAn_nnnn'nTGG
Cuts at: 2098 2098
Size: 5188
BstYI r'GATC_y
Cuts at: 1 979 1031 1743 1982 2211 2678 2701 3036
Size: 978 52 712 239 229 467 23 335
Cuts at: 3036 3282 4296 4382 4394 4740 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 978 712 467 449 346 335 246
239 229 86 52 23 12
BtgI C'CryG_G
Cuts at: 166 533 1596 166
Size: 367 1063 3758
Fragments arranged by size:
3758 1063 367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3136 3317 3480 3136
Size: 181 163 4844
Fragments arranged by size:
4844 181 163
BtsI GCAGTG_nn'
Cuts at: 640 2429 640
Size: 1789 3399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 543 578 543
Size: 35 5153
DraI TTT'AAA
Cuts at: 2185 2185
Size: 5188
DrdI GACnn_nn'nnGTC
Cuts at: 2892 3752 2892
Size: 860 4328
EaeI y'GGCC_r
Cuts at: 536 606 1341 1692 2771 2945 3336 3363 4710
Size: 70 735 351 1079 174 391 27 1347
Cuts at: 4710 536
Size: 1014
Fragments arranged by size:
1347 1079 1014 735 391 351 174 70
27
EagI C'GGCC_G
Cuts at: 2771 2771
Size: 5188
EarI CTCTTCn'nnn_
Cuts at: 1936 2075 2134 3209 3419 1936
Size: 139 59 1075 210 3705
Fragments arranged by size:
3705 1075 210 139 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3394 3706 3852 4998 3394
Size: 312 146 1146 3584
Fragments arranged by size:
3584 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1349 1733 1766 2198 1349
Size: 384 33 432 4339
Fragments arranged by size:
4339 432 384 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 1168 1224 1451 1658 1726 2173 3011 3443
Size: 691 56 227 207 68 447 838 432
Cuts at: 3443 4192 4460 477
Size: 749 268 1205
Fragments arranged by size:
1205 838 749 691 447 432 268 227
207 68 56
EcoNI CCTnn'n_nnAGG
Cuts at: 825 2074 825
Size: 1249 3939
EcoO109I rG'GnC_Cy
Cuts at: 1379 1601 2051 2325 1379
Size: 222 450 274 4242
Fragments arranged by size:
4242 450 274 222
EcoRI G'AATT_C
Cuts at: 2190 2190
Size: 5188
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 507 3205 507
Size: 2698 2490
FspI TGC'GCA
Cuts at: 2967 2967
Size: 5188
HaeII r_GCGC'y
Cuts at: 2067 2620 2868 3892 4871 2067
Size: 553 248 1024 979 2384
Fragments arranged by size:
2384 1024 979 553 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1363 1395 3028 3058 3060 3090 1363
Size: 32 1633 30 2 30 3461
Fragments arranged by size:
3461 1633 32 30 30 2
HincII GTy'rAC
Cuts at: 1959 2225 1959
Size: 266 4922
KasI G'GCGC_C
Cuts at: 2616 2864 2616
Size: 248 4940
KpnI G_GTAC'C
Cuts at: 2206 2206
Size: 5188
MluI A'CGCG_T
Cuts at: 4621 4621
Size: 5188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 749 1160 1414 3859 4043 749
Size: 411 254 2445 184 1894
Fragments arranged by size:
2445 1894 411 254 184
MscI TGG'CCA
Cuts at: 538 2947 538
Size: 2409 2779
MslI CAynn'nnrTG
Cuts at: 3175 3302 3175
Size: 127 5061
MspA1I CmG'CkG
Cuts at: 200 368 377 497 647 1429 1798 2297 2611
Size: 168 9 120 150 782 369 499 314
Cuts at: 2611 2971 3986 4231 200
Size: 360 1015 245 1157
Fragments arranged by size:
1157 1015 782 499 369 360 314 245
168 150 120 9
NaeI GCC'GGC
Cuts at: 1276 1696 3367 1276
Size: 420 1671 3097
Fragments arranged by size:
3097 1671 420
NarI GG'CG_CC
Cuts at: 2617 2865 2617
Size: 248 4940
NcoI C'CATG_G
Cuts at: 533 1596 533
Size: 1063 4125
NgoMIV G'CCGG_C
Cuts at: 1274 1694 3365 1274
Size: 420 1671 3097
Fragments arranged by size:
3097 1671 420
NruI TCG'CGA
Cuts at: 3549 3549
Size: 5188
NspI r_CATG'y
Cuts at: 1134 1287 1671 1971 2239 2739 3648 1134
Size: 153 384 300 268 500 909 2674
Fragments arranged by size:
2674 909 500 384 300 268 153
PasI CC'CwG_GG
Cuts at: 414 414
Size: 5188
PciI A'CATG_T
Cuts at: 1130 3644 1130
Size: 2514 2674
PflMI CCAn_nnn'nTGG
Cuts at: 534 534
Size: 5188
PmeI GTTT'AAAC
Cuts at: 2185 2185
Size: 5188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1170 1202 4327 4359 1170
Size: 32 3125 32 1999
Fragments arranged by size:
3125 1999 32 32
PpuMI rG'GwC_Cy
Cuts at: 1379 1601 2051 1379
Size: 222 450 4516
Fragments arranged by size:
4516 450 222
PspOMI G'GGCC_C
Cuts at: 1330 1330
Size: 5188
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 310 342 310
Size: 32 5156
PstI C_TGCA'G
Cuts at: 670 2233 2918 670
Size: 1563 685 2940
Fragments arranged by size:
2940 1563 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5188
PvuII CAG'CTG
Cuts at: 377 647 1429 1798 2611 2971 377
Size: 270 782 369 813 360 2594
Fragments arranged by size:
2594 813 782 369 360 270
RsrII CG'GwC_CG
Cuts at: 3381 3381
Size: 5188
SacI G_AGCT'C
Cuts at: 1351 1735 1768 2200 1351
Size: 384 33 432 4339
Fragments arranged by size:
4339 432 384 33
SalI G'TCGA_C
Cuts at: 2223 2223
Size: 5188
SanDI GG'GwC_CC
Cuts at: 1601 1601
Size: 5188
SapI GCTCTTCn'nnn_
Cuts at: 3209 3419 3209
Size: 210 4978
SbfI CC_TGCA'GG
Cuts at: 2233 2233
Size: 5188
ScaI AGT'ACT
Cuts at: 1585 4705 1585
Size: 3120 2068
SfcI C'TryA_G
Cuts at: 33 666 1269 1487 2229 2914 3909 4100 33
Size: 633 603 218 742 685 995 191 1121
Fragments arranged by size:
1121 995 742 685 633 603 218 191
SfoI GGC'GCC
Cuts at: 2618 2866 2618
Size: 248 4940
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5188
SmaI CCC'GGG
Cuts at: 922 1247 2208 922
Size: 325 961 3902
Fragments arranged by size:
3902 961 325
SmlI C'TyrA_G
Cuts at: 2341 3256 3750 4012 2341
Size: 915 494 262 3517
Fragments arranged by size:
3517 915 494 262
SnaBI TAC'GTA
Cuts at: 2178 2178
Size: 5188
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5188
SphI G_CATG'C
Cuts at: 1671 1971 2239 2739 1671
Size: 300 268 500 4120
Fragments arranged by size:
4120 500 300 268
SrfI GCCC'GGGC
Cuts at: 922 922
Size: 5188
StyI C'CwwG_G
Cuts at: 533 1064 1596 2036 2320 2674 533
Size: 531 532 440 284 354 3047
Fragments arranged by size:
3047 532 531 440 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 782 830 1011 1820 1932 2769 2902 3361
Size: 430 48 181 809 112 837 133 459
Cuts at: 3361 3572 352
Size: 211 1968
Fragments arranged by size:
1968 837 809 459 430 211 181 133
112 48
TatI w'GTAC_w
Cuts at: 326 1535 1583 1976 4703 326
Size: 1209 48 393 2727 811
Fragments arranged by size:
2727 1209 811 393 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 123 155 470 502 619 651 4705 4737 123
Size: 32 315 32 117 32 4054 32 574
Fragments arranged by size:
4054 574 315 117 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2983 2983
Size: 5188
XbaI T'CTAG_A
Cuts at: 2217 2217
Size: 5188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 355 739 1687 2681 355
Size: 384 948 994 2862
Fragments arranged by size:
2862 994 948 384
XmaI C'CCGG_G
Cuts at: 920 1245 2206 920
Size: 325 961 3902
Fragments arranged by size:
3902 961 325
XmnI GAAnn'nnTTC
Cuts at: 466 466
Size: 5188
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BbvCI BfrBI BmtI Bpu10I BsiWI BsrGI BssHII BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PfoI PmlI
PshAI PsiI PspXI SacII SexAI SfiI SgrAI SspI
StuI SwaI XhoI ZraI