Restriction map for
pF1KSDA0833
(Circular) (Six-base) MAPSORT of: pF1KSDA0833.seq Check: 5071 from: 1 to: 8158
pF1KSDA0833 8158 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3629 3629
Size: 8158
AccI GT'mk_AC
Cuts at: 4117 5194 4117
Size: 1077 7081
Acc65I G'GTAC_C
Cuts at: 5172 5172
Size: 8158
AclI AA'CG_TT
Cuts at: 7998 7998
Size: 8158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1674 1866 1905 2415 3996 5981 6413 7162
Size: 1058 192 39 510 1581 1985 432 749
Cuts at: 7162 7430 616
Size: 268 1344
Fragments arranged by size:
1985 1581 1344 1058 749 510 432 268
192 39
AflIII A'CryG_T
Cuts at: 1046 1502 1910 5508 6614 7591 1046
Size: 456 408 3598 1106 977 1613
Fragments arranged by size:
3598 1613 1106 977 456 408
AgeI A'CCGG_T
Cuts at: 6546 6546
Size: 8158
AhdI GACnn_n'nnGTC
Cuts at: 1911 3027 1911
Size: 1116 7042
AleI CACnn'nnGTG
Cuts at: 1264 1501 2050 1264
Size: 237 549 7372
Fragments arranged by size:
7372 549 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2362 2598 2362
Size: 236 7922
AlwNI CAG_nnn'CTG
Cuts at: 2096 2860 4045 4414 5461 7030 2096
Size: 764 1185 369 1047 1569 3224
Fragments arranged by size:
3224 1569 1185 1047 764 369
ApaI G_GGCC'C
Cuts at: 2562 2562
Size: 8158
ApaLI G'TGCA_C
Cuts at: 655 6928 655
Size: 6273 1885
ApoI r'AATT_y
Cuts at: 4089 4371 4615 4864 4891 5160 7511 7767 4089
Size: 282 244 249 27 269 2351 256 4480
Fragments arranged by size:
4480 2351 282 269 256 249 244 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 8158
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8158
AvaI C'yCGr_G
Cuts at: 1675 1965 2334 2987 3976 5176 1675
Size: 290 369 653 989 1200 4657
Fragments arranged by size:
4657 1200 989 653 369 290
AvrII C'CTAG_G
Cuts at: 2706 3483 2706
Size: 777 7381
BamHI G'GATC_C
Cuts at: 5181 5181
Size: 8158
BanI G'GyrC_C
Cuts at: 338 2044 2501 2701 5172 5586 5834 5869 338
Size: 1706 457 200 2471 414 248 35 2627
Fragments arranged by size:
2627 2471 1706 457 414 248 200 35
BanII G_rGCy'C
Cuts at: 767 1087 1198 1223 1435 2405 2562 2994 3689
Size: 320 111 25 212 970 157 432 695
Cuts at: 3689 4546 5170 6200 767
Size: 857 624 1030 2725
Fragments arranged by size:
2725 1030 970 857 695 624 432 320
212 157 111 25
BbeI G_GCGC'C
Cuts at: 5590 5838 5590
Size: 248 7910
BbsI GAAGACnn'nnnn_
Cuts at: 3798 3798
Size: 8158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 276 310 276
Size: 34 8124
BciVI GTATCCnnnnn_n'
Cuts at: 6054 6817 8158 6054
Size: 763 1341 6054
Fragments arranged by size:
6054 1341 763
BclI T'GATC_A
Cuts at: 243 2453 4677 5676 243
Size: 2210 2224 999 2725
Fragments arranged by size:
2725 2224 2210 999
BglI GCCn_nnn'nGGC
Cuts at: 1931 2179 2692 3277 4550 1931
Size: 248 513 585 1273 5539
Fragments arranged by size:
5539 1273 585 513 248
BglII A'GATC_T
Cuts at: 2849 4098 5671 7710 2849
Size: 1249 1573 2039 3297
Fragments arranged by size:
3297 2039 1573 1249
BlpI GC'TnA_GC
Cuts at: 2404 2623 3641 4661 5268 2404
Size: 219 1018 1020 607 5294
Fragments arranged by size:
5294 1020 1018 607 219
Bme1580I G_kGCm'C
Cuts at: 659 2047 2504 2562 2704 3483 3491 4382 5781
Size: 1388 457 58 142 779 8 891 1399
Cuts at: 5781 5874 6932 659
Size: 93 1058 1885
Fragments arranged by size:
1885 1399 1388 1058 891 779 457 142
93 58 8
BmgBI CAC'GTC
Cuts at: 1047 1913 2667 1047
Size: 866 754 6538
Fragments arranged by size:
6538 866 754
BmrI ACTGGGnnnn_n'
Cuts at: 3215 3705 3986 4472 5368 5449 5784 7930 3215
Size: 490 281 486 896 81 335 2146 3443
Fragments arranged by size:
3443 2146 896 490 486 335 281 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1173 1752 2697 3812 6523 1173
Size: 579 945 1115 2711 2808
Fragments arranged by size:
2808 2711 1115 945 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1071 1806 1888 3094 3634 3722 4022 1071
Size: 735 82 1206 540 88 300 5207
Fragments arranged by size:
5207 1206 735 540 300 88 82
Bpu10I CC'TnA_GC
Cuts at: 831 831
Size: 8158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 2224 4560 5332 6211 6705 7003 529
Size: 1695 2336 772 879 494 298 1684
Fragments arranged by size:
2336 1695 1684 879 772 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1158 2197 2284 2685 7862 1158
Size: 1039 87 401 5177 1454
Fragments arranged by size:
5177 1454 1039 401 87
BsaAI yAC'GTr
Cuts at: 2109 5148 5509 2109
Size: 3039 361 4758
Fragments arranged by size:
4758 3039 361
BsaBI GATnn'nnATC
Cuts at: 6 3058 5670 7709 6
Size: 3052 2612 2039 455
Fragments arranged by size:
3052 2612 2039 455
BsaHI Gr'CG_yC
Cuts at: 1403 1985 2321 5587 5835 6539 8035 1403
Size: 582 336 3266 248 704 1496 1526
Fragments arranged by size:
3266 1526 1496 704 582 336 248
BsaWI w'CCGG_w
Cuts at: 611 1270 4730 5345 5866 6546 6820 6967 7378
Size: 659 3460 615 521 680 274 147 411
Cuts at: 7378 611
Size: 1391
Fragments arranged by size:
3460 1391 680 659 615 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 1759 1789 2065 2095 2428 2452 2458
Size: 30 1176 30 276 30 333 24 6
Cuts at: 2458 2482 2774 2804 3436 3466 3746 3776 4579
Size: 24 292 30 632 30 280 30 803
Cuts at: 4579 4609 553
Size: 30 4102
Fragments arranged by size:
4102 1176 803 632 333 292 280 276
30 30 30 30 30 30 30 24
24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1146 1381 1761 1764 2795 3102 3452 1146
Size: 235 380 3 1031 307 350 5852
Fragments arranged by size:
5852 1031 380 350 307 235 3
BseYI C'CCAG_C
Cuts at: 890 1855 2369 2374 2603 2901 3014 3113 3275
Size: 965 514 5 229 298 113 99 162
Cuts at: 3275 3645 3688 4245 5581 6339 6918 7414 890
Size: 370 43 557 1336 758 579 496 1634
Fragments arranged by size:
1634 1336 965 758 579 557 514 496
370 298 229 162 113 99 43 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 900 1054 1117 2368 3899 4286 877
Size: 23 154 63 1251 1531 387 4749
Fragments arranged by size:
4749 1531 1251 387 154 63 23
BsiEI CG_ry'CG
Cuts at: 104 2687 5698 5744 6954 104
Size: 2583 3011 46 1210 1308
Fragments arranged by size:
3011 2583 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 659 767 1070 1130 1198 1435 2276 2405 3620
Size: 108 303 60 68 237 841 129 1215
Cuts at: 3620 4922 5170 5948 6138 6932 659
Size: 1302 248 778 190 794 1885
Fragments arranged by size:
1885 1302 1215 841 794 778 303 248
237 190 129 108 68 60
BsiWI C'GTAC_G
Cuts at: 1616 1616
Size: 8158
BsmI GAATG_Cn'
Cuts at: 4285 4285
Size: 8158
BsmBI CGTCTCn'nnnn_
Cuts at: 2107 2107
Size: 8158
Bsp1286I G_dGCh'C
Cuts at: 659 767 1070 1087 1130 1198 1223 1435 2047
Size: 108 303 17 43 68 25 212 612
Cuts at: 2047 2276 2405 2504 2562 2704 2994 3483 3491
Size: 229 129 99 58 142 290 489 8
Cuts at: 3491 3620 3689 4382 4546 4922 5170 5781 5874
Size: 129 69 693 164 376 248 611 93
Cuts at: 5874 5948 6138 6200 6932 659
Size: 74 190 62 732 1885
Fragments arranged by size:
1885 732 693 612 611 489 376 303
290 248 229 212 190 164 142 129
129 108 99 93 74 69 68 62
58 43 25 17 8
BspEI T'CCGG_A
Cuts at: 4730 7378 4730
Size: 2648 5510
BspHI T'CATG_A
Cuts at: 4680 7334 4680
Size: 2654 5504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 817 2288 3629 5206 5420 5722 6103 817
Size: 1471 1341 1577 214 302 381 2872
Fragments arranged by size:
2872 1577 1471 1341 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1683 2726 3134 3167 6448 6502 7988 8153
Size: 1633 1043 408 33 3281 54 1486 165
Cuts at: 8153 50
Size: 55
Fragments arranged by size:
3281 1633 1486 1043 408 165 55 54
33
BsrDI GCAATG_nn'
Cuts at: 822 6068 822
Size: 5246 2912
BsrFI r'CCGG_y
Cuts at: 672 919 1816 2314 6154 6335 6546 672
Size: 247 897 498 3840 181 211 2284
Fragments arranged by size:
3840 2284 897 498 247 211 181
BsrGI T'GTAC_A
Cuts at: 1352 3512 4992 5105 1352
Size: 2160 1480 113 4405
Fragments arranged by size:
4405 2160 1480 113
BssHII G'CGCG_C
Cuts at: 132 132
Size: 8158
BssSI C'ACGA_G
Cuts at: 1063 6427 6787 1063
Size: 5364 360 2434
Fragments arranged by size:
5364 2434 360
BstAPI GCAn_nnn'nTGC
Cuts at: 698 2280 3193 5533 698
Size: 1582 913 2340 3323
Fragments arranged by size:
3323 2340 1582 913
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8158
BstEII G'GTnAC_C
Cuts at: 1335 2193 2742 1335
Size: 858 549 6751
Fragments arranged by size:
6751 858 549
BstXI CCAn_nnnn'nTGG
Cuts at: 1170 2652 2914 2967 1170
Size: 1482 262 53 6361
Fragments arranged by size:
6361 1482 262 53
BstYI r'GATC_y
Cuts at: 1 863 2849 4066 4098 4490 5181 5648 5671
Size: 862 1986 1217 32 392 691 467 23
Cuts at: 5671 6006 6252 7266 7352 7364 7710 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1986 1217 1014 862 691 467 449 392
346 335 246 86 32 23 12
Bsu36I CC'TnA_GG
Cuts at: 4392 4392
Size: 8158
BtgI C'CryG_G
Cuts at: 792 895 1000 1316 1463 1778 2060 2198 2363
Size: 103 105 316 147 315 282 138 165
Cuts at: 2363 2471 2651 2806 3315 4189 4292 4382 4601
Size: 108 180 155 509 874 103 90 219
Cuts at: 4601 792
Size: 4349
Fragments arranged by size:
4349 874 509 316 315 282 219 180
165 155 147 138 108 105 103 103
90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6106 6287 6450 6106
Size: 181 163 7814
Fragments arranged by size:
7814 181 163
BtsI GCAGTG_nn'
Cuts at: 3153 3831 3911 5399 3153
Size: 678 80 1488 5912
Fragments arranged by size:
5912 1488 678 80
DraI TTT'AAA
Cuts at: 5155 5155
Size: 8158
DraIII CAC_nnn'GTG
Cuts at: 3193 3193
Size: 8158
DrdI GACnn_nn'nnGTC
Cuts at: 5862 6722 5862
Size: 860 7298
EaeI y'GGCC_r
Cuts at: 674 1115 1424 1440 1542 1754 2684 3095 3338
Size: 441 309 16 102 212 930 411 243
Cuts at: 3338 4284 4445 5741 5915 6306 6333 7680 674
Size: 946 161 1296 174 391 27 1347 1152
Fragments arranged by size:
1347 1296 1152 946 930 441 411 391
309 243 212 174 161 102 27 16
EagI C'GGCC_G
Cuts at: 2684 5741 2684
Size: 3057 5101
EarI CTCTTCn'nnn_
Cuts at: 163 755 1093 1505 3677 3923 6179 6389 163
Size: 592 338 412 2172 246 2256 210 1932
Fragments arranged by size:
2256 2172 1932 592 412 338 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2233 4587 6364 6676 6822 7968 2233
Size: 2354 1777 312 146 1146 2423
Fragments arranged by size:
2423 2354 1777 1146 312 146
EcoICRI GAG'CTC
Cuts at: 765 1196 1433 2403 5168 765
Size: 431 237 970 2765 3755
Fragments arranged by size:
3755 2765 970 431 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1071 1674 1806 1866 1888 1905 2415 3094
Size: 455 603 132 60 22 17 510 679
Cuts at: 3094 3634 3722 3996 4022 5981 6413 7162 7430
Size: 540 88 274 26 1959 432 749 268
Cuts at: 7430 616
Size: 1344
Fragments arranged by size:
1959 1344 749 679 603 540 510 455
432 274 268 132 88 60 26 22
17
EcoNI CCTnn'n_nnAGG
Cuts at: 1711 1711
Size: 8158
EcoO109I rG'GnC_Cy
Cuts at: 1413 2232 2559 2790 3110 3209 4250 5295 1413
Size: 819 327 231 320 99 1041 1045 4276
Fragments arranged by size:
4276 1045 1041 819 327 320 231 99
EcoRI G'AATT_C
Cuts at: 4089 4615 5160 4089
Size: 526 545 7087
Fragments arranged by size:
7087 545 526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1470 3581 6175 1470
Size: 2111 2594 3453
Fragments arranged by size:
3453 2594 2111
FspI TGC'GCA
Cuts at: 5937 5937
Size: 8158
HaeII r_GCGC'y
Cuts at: 968 1316 1889 2315 2533 5590 5838 6862 7841
Size: 348 573 426 218 3057 248 1024 979
Cuts at: 7841 968
Size: 1285
Fragments arranged by size:
3057 1285 1024 979 573 426 348 248
218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1468 1500 1897 1929 2065 2097 2452 2484 2774
Size: 32 397 32 136 32 355 32 290
Cuts at: 2774 2806 2836 2868 3013 3045 3436 3468 4122
Size: 32 30 32 145 32 391 32 654
Cuts at: 4122 4154 4234 4266 5998 6028 6030 6060 1468
Size: 32 80 32 1732 30 2 30 3566
Fragments arranged by size:
3566 1732 654 397 391 355 290 145
136 80 32 32 32 32 32 32
32 32 32 32 30 30 30 2
HincII GTy'rAC
Cuts at: 3429 5195 3429
Size: 1766 6392
KasI G'GCGC_C
Cuts at: 5586 5834 5586
Size: 248 7910
KpnI G_GTAC'C
Cuts at: 5176 5176
Size: 8158
MluI A'CGCG_T
Cuts at: 7591 7591
Size: 8158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1679 1922 2417 2471 2984 3014 6829 7013
Size: 648 243 495 54 513 30 3815 184
Cuts at: 7013 1031
Size: 2176
Fragments arranged by size:
3815 2176 648 513 495 243 184 54
30
MscI TGG'CCA
Cuts at: 1117 1442 1756 4447 5917 1117
Size: 325 314 2691 1470 3358
Fragments arranged by size:
3358 2691 1470 325 314
MslI CAynn'nnrTG
Cuts at: 1264 1501 2050 2470 4111 4306 6145 6272 1264
Size: 237 549 420 1641 195 1839 127 3150
Fragments arranged by size:
3150 1839 1641 549 420 237 195 127
MspA1I CmG'CkG
Cuts at: 344 777 852 897 1217 1372 1780 2102 2508
Size: 433 75 45 320 155 408 322 406
Cuts at: 2508 2622 2834 3161 3317 3343 3645 4603 5072
Size: 114 212 327 156 26 302 958 469
Cuts at: 5072 5267 5581 5941 6956 7201 344
Size: 195 314 360 1015 245 1301
Fragments arranged by size:
1301 1015 958 469 433 408 406 360
327 322 320 314 302 245 212 195
156 155 114 75 45 26
NaeI GCC'GGC
Cuts at: 674 1818 2316 6337 674
Size: 1144 498 4021 2495
Fragments arranged by size:
4021 2495 1144 498
NarI GG'CG_CC
Cuts at: 5587 5835 5587
Size: 248 7910
NcoI C'CATG_G
Cuts at: 792 2198 2651 4292 4382 792
Size: 1406 453 1641 90 4568
Fragments arranged by size:
4568 1641 1406 453 90
NgoMIV G'CCGG_C
Cuts at: 672 1816 2314 6335 672
Size: 1144 498 4021 2495
Fragments arranged by size:
4021 2495 1144 498
NruI TCG'CGA
Cuts at: 6519 6519
Size: 8158
NspI r_CATG'y
Cuts at: 2664 2715 5209 5709 6618 2664
Size: 51 2494 500 909 4204
Fragments arranged by size:
4204 2494 909 500 51
PasI CC'CwG_GG
Cuts at: 2697 3348 2697
Size: 651 7507
PciI A'CATG_T
Cuts at: 6614 6614
Size: 8158
PflMI CCAn_nnn'nTGG
Cuts at: 436 558 4293 436
Size: 122 3735 4301
Fragments arranged by size:
4301 3735 122
PfoI T'CCnGG_A
Cuts at: 3062 3062
Size: 8158
PmeI GTTT'AAAC
Cuts at: 5155 5155
Size: 8158
PmlI CAC'GTG
Cuts at: 2109 2109
Size: 8158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 814 846 7297 7329 814
Size: 32 6451 32 1643
Fragments arranged by size:
6451 1643 32 32
PpuMI rG'GwC_Cy
Cuts at: 2232 2790 3110 4250 2232
Size: 558 320 1140 6140
Fragments arranged by size:
6140 1140 558 320
PshAI GACnn'nnGTC
Cuts at: 2915 2915
Size: 8158
PsiI TTA'TAA
Cuts at: 4826 4946 4826
Size: 120 8038
PspOMI G'GGCC_C
Cuts at: 2558 2558
Size: 8158
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3862 3894 3862
Size: 32 8126
PstI C_TGCA'G
Cuts at: 814 1982 2101 2258 2285 2621 2720 3626 4050
Size: 1168 119 157 27 336 99 906 424
Cuts at: 4050 5203 5888 814
Size: 1153 685 3084
Fragments arranged by size:
3084 1168 1153 906 685 424 336 157
119 99 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8158
PvuII CAG'CTG
Cuts at: 777 852 2508 2622 2834 3645 5581 5941 777
Size: 75 1656 114 212 811 1936 360 2994
Fragments arranged by size:
2994 1936 1656 811 360 212 114 75
RsrII CG'GwC_CG
Cuts at: 6351 6351
Size: 8158
SacI G_AGCT'C
Cuts at: 767 1198 1435 2405 5170 767
Size: 431 237 970 2765 3755
Fragments arranged by size:
3755 2765 970 431 237
SacII CC_GC'GG
Cuts at: 898 1781 3318 4604 898
Size: 883 1537 1286 4452
Fragments arranged by size:
4452 1537 1286 883
SalI G'TCGA_C
Cuts at: 5193 5193
Size: 8158
SanDI GG'GwC_CC
Cuts at: 3110 3110
Size: 8158
SapI GCTCTTCn'nnn_
Cuts at: 163 755 1505 3677 3923 6179 6389 163
Size: 592 750 2172 246 2256 210 1932
Fragments arranged by size:
2256 2172 1932 750 592 246 210
SbfI CC_TGCA'GG
Cuts at: 1982 5203 1982
Size: 3221 4937
ScaI AGT'ACT
Cuts at: 3880 4933 7675 3880
Size: 1053 2742 4363
Fragments arranged by size:
4363 2742 1053
SexAI A'CCwGG_T
Cuts at: 1166 1166
Size: 8158
SfcI C'TryA_G
Cuts at: 33 233 810 1978 2097 2254 2281 2595 2617
Size: 200 577 1168 119 157 27 314 22
Cuts at: 2617 2716 2829 3622 3819 4046 4076 4118 5199
Size: 99 113 793 197 227 30 42 1081
Cuts at: 5199 5884 6879 7070 33
Size: 685 995 191 1121
Fragments arranged by size:
1168 1121 1081 995 793 685 577 314
227 200 197 191 157 119 113 99
42 30 27 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 2692 2692
Size: 8158
SfoI GGC'GCC
Cuts at: 5588 5836 5588
Size: 248 7910
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8158
SgrAI Cr'CCGG_yG
Cuts at: 2314 2314
Size: 8158
SmaI CCC'GGG
Cuts at: 1677 2336 2989 5178 1677
Size: 659 653 2189 4657
Fragments arranged by size:
4657 2189 659 653
SmlI C'TyrA_G
Cuts at: 544 2239 4539 5311 6226 6720 6982 544
Size: 1695 2300 772 915 494 262 1720
Fragments arranged by size:
2300 1720 1695 915 772 494 262
SnaBI TAC'GTA
Cuts at: 5148 5148
Size: 8158
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8158
SphI G_CATG'C
Cuts at: 2715 5209 5709 2715
Size: 2494 500 5164
Fragments arranged by size:
5164 2494 500
SrfI GCCC'GGGC
Cuts at: 2989 2989
Size: 8158
StuI AGG'CCT
Cuts at: 1631 2077 1631
Size: 446 7712
StyI C'CwwG_G
Cuts at: 792 989 1712 2198 2651 2706 3212 3483 3749
Size: 197 723 486 453 55 506 271 266
Cuts at: 3749 4292 4322 4382 5136 5290 5644 792
Size: 543 30 60 754 154 354 3306
Fragments arranged by size:
3306 754 723 543 506 486 453 354
271 266 197 154 60 55 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1204 1741 2032 2584 2761 4370 5739 5872 6331
Size: 537 291 552 177 1609 1369 133 459
Cuts at: 6331 6542 1204
Size: 211 2820
Fragments arranged by size:
2820 1609 1369 552 537 459 291 211
177 133
TatI w'GTAC_w
Cuts at: 1352 2978 3457 3512 3878 4122 4429 4931 4992
Size: 1626 479 55 366 244 307 502 61
Cuts at: 4992 5105 7673 1352
Size: 113 2568 1837
Fragments arranged by size:
2568 1837 1626 502 479 366 307 244
113 61 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7675 7707 7675
Size: 32 8126
Tth111I GACn'n_nGTC
Cuts at: 646 3108 5953 646
Size: 2462 2845 2851
Fragments arranged by size:
2851 2845 2462
XbaI T'CTAG_A
Cuts at: 5187 5187
Size: 8158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1167 1863 5651 1167
Size: 696 3788 3674
Fragments arranged by size:
3788 3674 696
XmaI C'CCGG_G
Cuts at: 1675 2334 2987 5176 1675
Size: 659 653 2189 4657
Fragments arranged by size:
4657 2189 659 653
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI BaeI BbvCI BfrBI
BmtI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PspXI
SspI SwaI XhoI XmnI ZraI