Restriction map for
pF1KSDA0794
(Circular) (Six-base) MAPSORT of: pF1KSDA0794.seq Check: 7806 from: 1 to: 4606
pF1KSDA0794 4606 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1642 1642
Size: 4606
Acc65I G'GTAC_C
Cuts at: 1620 1620
Size: 4606
AclI AA'CG_TT
Cuts at: 1476 4446 1476
Size: 2970 1636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 314 2429 2861 3610 3878 184
Size: 130 2115 432 749 268 912
Fragments arranged by size:
2115 912 749 432 268 130
AflIII A'CryG_T
Cuts at: 1956 3062 4039 1956
Size: 1106 977 2523
Fragments arranged by size:
2523 1106 977
AgeI A'CCGG_T
Cuts at: 196 2994 196
Size: 2798 1808
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 684 684
Size: 4606
AlwNI CAG_nnn'CTG
Cuts at: 1909 3478 1909
Size: 1569 3037
ApaLI G'TGCA_C
Cuts at: 3376 3376
Size: 4606
ApoI r'AATT_y
Cuts at: 1608 3959 4215 1608
Size: 2351 256 1999
Fragments arranged by size:
2351 1999 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4606
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4606
AvaI C'yCGr_G
Cuts at: 1624 1624
Size: 4606
BamHI G'GATC_C
Cuts at: 1629 1629
Size: 4606
BanI G'GyrC_C
Cuts at: 851 1620 2034 2282 2317 851
Size: 769 414 248 35 3140
Fragments arranged by size:
3140 769 414 248 35
BanII G_rGCy'C
Cuts at: 145 302 1618 2648 145
Size: 157 1316 1030 2103
Fragments arranged by size:
2103 1316 1030 157
BbeI G_GCGC'C
Cuts at: 2038 2286 2038
Size: 248 4358
BbsI GAAGACnn'nnnn_
Cuts at: 989 989
Size: 4606
BciVI GTATCCnnnnn_n'
Cuts at: 1391 1456 2502 3265 4606 1391
Size: 65 1046 763 1341 1391
Fragments arranged by size:
1391 1341 1046 763 65
BclI T'GATC_A
Cuts at: 2124 2124
Size: 4606
BfrBI ATG'CAT
Cuts at: 573 573
Size: 4606
BglII A'GATC_T
Cuts at: 543 2119 4158 543
Size: 1576 2039 991
Fragments arranged by size:
2039 1576 991
BlpI GC'TnA_GC
Cuts at: 1716 1716
Size: 4606
Bme1580I G_kGCm'C
Cuts at: 363 954 2229 2322 3380 363
Size: 591 1275 93 1058 1589
Fragments arranged by size:
1589 1275 1058 591 93
BmrI ACTGGGnnnn_n'
Cuts at: 296 1816 1897 2232 4378 296
Size: 1520 81 335 2146 524
Fragments arranged by size:
2146 1520 524 335 81
BmtI G_CTAG'C
Cuts at: 1435 1435
Size: 4606
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 1321
Size: 4606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1780 2659 3153 3451 1547
Size: 233 879 494 298 2702
Fragments arranged by size:
2702 879 494 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 4310 4310
Size: 4606
BsaAI yAC'GTr
Cuts at: 1449 1596 1957 1449
Size: 147 361 4098
Fragments arranged by size:
4098 361 147
BsaBI GATnn'nnATC
Cuts at: 6 2118 4157 6
Size: 2112 2039 455
Fragments arranged by size:
2112 2039 455
BsaHI Gr'CG_yC
Cuts at: 151 2035 2283 2987 4483 151
Size: 1884 248 704 1496 274
Fragments arranged by size:
1884 1496 704 274 248
BsaWI w'CCGG_w
Cuts at: 196 1793 2314 2994 3268 3415 3826 196
Size: 1597 521 680 274 147 411 976
Fragments arranged by size:
1597 976 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 127
Size: 30 4576
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 524 1120 1219 524
Size: 596 99 3911
Fragments arranged by size:
3911 596 99
BseYI C'CCAG_C
Cuts at: 2029 2787 3366 3862 2029
Size: 758 579 496 2773
Fragments arranged by size:
2773 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 1471 419
Size: 1052 3554
BsiEI CG_ry'CG
Cuts at: 104 2146 2192 3402 104
Size: 2042 46 1210 1308
Fragments arranged by size:
2042 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 417 1618 2396 2586 3380 417
Size: 1201 778 190 794 1643
Fragments arranged by size:
1643 1201 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 145 302 363 417 954 1618 2229 2322 2396
Size: 157 61 54 537 664 611 93 74
Cuts at: 2396 2586 2648 3380 145
Size: 190 62 732 1371
Fragments arranged by size:
1371 732 664 611 537 190 157 93
74 62 61 54
BspEI T'CCGG_A
Cuts at: 3826 3826
Size: 4606
BspHI T'CATG_A
Cuts at: 747 3782 747
Size: 3035 1571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 729 1654 1868 2170 2551 729
Size: 925 214 302 381 2784
Fragments arranged by size:
2784 925 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1064 2896 2950 4436 4601 50
Size: 1014 1832 54 1486 165 55
Fragments arranged by size:
1832 1486 1014 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1537 2516 1537
Size: 979 3627
BsrFI r'CCGG_y
Cuts at: 196 2602 2783 2994 196
Size: 2406 181 211 1808
Fragments arranged by size:
2406 1808 211 181
BsrGI T'GTAC_A
Cuts at: 1578 1578
Size: 4606
BssSI C'ACGA_G
Cuts at: 2875 3235 2875
Size: 360 4246
BstAPI GCAn_nnn'nTGC
Cuts at: 1981 1981
Size: 4606
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4606
BstYI r'GATC_y
Cuts at: 1 543 1629 2096 2119 2454 2700 3714 3800
Size: 542 1086 467 23 335 246 1014 86
Cuts at: 3800 3812 4158 1
Size: 12 346 449
Fragments arranged by size:
1086 1014 542 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 135 146 823 125
Size: 10 11 677 3908
Fragments arranged by size:
3908 677 11 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 695 2554 2735 2898 695
Size: 1859 181 163 2403
Fragments arranged by size:
2403 1859 181 163
BtsI GCAGTG_nn'
Cuts at: 1107 1847 1107
Size: 740 3866
ClaI AT'CG_AT
Cuts at: 518 518
Size: 4606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 585 604 620 639 585
Size: 19 16 19 4552
Fragments arranged by size:
4552 19 19 16
DraI TTT'AAA
Cuts at: 1603 1603
Size: 4606
DrdI GACnn_nn'nnGTC
Cuts at: 2310 3170 2310
Size: 860 3746
EaeI y'GGCC_r
Cuts at: 553 606 2189 2363 2754 2781 4128 553
Size: 53 1583 174 391 27 1347 1031
Fragments arranged by size:
1583 1347 1031 391 174 53 27
EagI C'GGCC_G
Cuts at: 2189 2189
Size: 4606
EarI CTCTTCn'nnn_
Cuts at: 290 1136 1415 2627 2837 290
Size: 846 279 1212 210 2059
Fragments arranged by size:
2059 1212 846 279 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2812 3124 3270 4416 2812
Size: 312 146 1146 3002
Fragments arranged by size:
3002 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1616 1616
Size: 4606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 314 1321 2429 2861 3610 3878 184
Size: 130 1007 1108 432 749 268 912
Fragments arranged by size:
1108 1007 912 749 432 268 130
EcoO109I rG'GnC_Cy
Cuts at: 1743 1743
Size: 4606
EcoRI G'AATT_C
Cuts at: 1608 1608
Size: 4606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4606
FspI TGC'GCA
Cuts at: 2385 2385
Size: 4606
HaeII r_GCGC'y
Cuts at: 163 2038 2286 3310 4289 163
Size: 1875 248 1024 979 480
Fragments arranged by size:
1875 1024 979 480 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 500 532 1048 1060 1080 1092 1099 1131 2446
Size: 32 516 12 20 12 7 32 1315
Cuts at: 2446 2476 2478 2508 500
Size: 30 2 30 2598
Fragments arranged by size:
2598 1315 516 32 32 30 30 20
12 12 7 2
HincII GTy'rAC
Cuts at: 1643 1643
Size: 4606
KasI G'GCGC_C
Cuts at: 2034 2282 2034
Size: 248 4358
KpnI G_GTAC'C
Cuts at: 1624 1624
Size: 4606
MluI A'CGCG_T
Cuts at: 4039 4039
Size: 4606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3277 3461 3277
Size: 184 4422
MscI TGG'CCA
Cuts at: 608 2365 608
Size: 1757 2849
MslI CAynn'nnrTG
Cuts at: 666 752 2593 2720 666
Size: 86 1841 127 2552
Fragments arranged by size:
2552 1841 127 86
MspA1I CmG'CkG
Cuts at: 148 1221 1282 1368 1715 2029 2389 3404 3649
Size: 1073 61 86 347 314 360 1015 245
Cuts at: 3649 148
Size: 1105
Fragments arranged by size:
1105 1073 1015 360 347 314 245 86
61
NaeI GCC'GGC
Cuts at: 2785 2785
Size: 4606
NarI GG'CG_CC
Cuts at: 2035 2283 2035
Size: 248 4358
NcoI C'CATG_G
Cuts at: 125 125
Size: 4606
NdeI CA'TA_TG
Cuts at: 224 224
Size: 4606
NgoMIV G'CCGG_C
Cuts at: 2783 2783
Size: 4606
NheI G'CTAG_C
Cuts at: 1431 1431
Size: 4606
NruI TCG'CGA
Cuts at: 2967 2967
Size: 4606
NsiI A_TGCA'T
Cuts at: 575 575
Size: 4606
NspI r_CATG'y
Cuts at: 665 1657 2157 3066 665
Size: 992 500 909 2205
Fragments arranged by size:
2205 992 909 500
PciI A'CATG_T
Cuts at: 3062 3062
Size: 4606
PflMI CCAn_nnn'nTGG
Cuts at: 409 409
Size: 4606
PfoI T'CCnGG_A
Cuts at: 458 716 1248 458
Size: 258 532 3816
Fragments arranged by size:
3816 532 258
PmeI GTTT'AAAC
Cuts at: 1603 1603
Size: 4606
PmlI CAC'GTG
Cuts at: 1449 1449
Size: 4606
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3745 3777 3745
Size: 32 4574
PsiI TTA'TAA
Cuts at: 785 785
Size: 4606
PstI C_TGCA'G
Cuts at: 1651 2336 1651
Size: 685 3921
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4606
PvuII CAG'CTG
Cuts at: 1282 1368 2029 2389 1282
Size: 86 661 360 3499
Fragments arranged by size:
3499 661 360 86
RsrII CG'GwC_CG
Cuts at: 2799 2799
Size: 4606
SacI G_AGCT'C
Cuts at: 1618 1618
Size: 4606
SacII CC_GC'GG
Cuts at: 149 149
Size: 4606
SalI G'TCGA_C
Cuts at: 1641 1641
Size: 4606
SapI GCTCTTCn'nnn_
Cuts at: 290 2627 2837 290
Size: 2337 210 2059
Fragments arranged by size:
2337 2059 210
SbfI CC_TGCA'GG
Cuts at: 1651 1651
Size: 4606
ScaI AGT'ACT
Cuts at: 4123 4123
Size: 4606
SfcI C'TryA_G
Cuts at: 33 1647 2332 3327 3518 33
Size: 1614 685 995 191 1121
Fragments arranged by size:
1614 1121 995 685 191
SfoI GGC'GCC
Cuts at: 2036 2284 2036
Size: 248 4358
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4606
SmaI CCC'GGG
Cuts at: 1626 1626
Size: 4606
SmlI C'TyrA_G
Cuts at: 1562 1759 2674 3168 3430 1562
Size: 197 915 494 262 2738
Fragments arranged by size:
2738 915 494 262 197
SnaBI TAC'GTA
Cuts at: 1596 1596
Size: 4606
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4606
SphI G_CATG'C
Cuts at: 1657 2157 1657
Size: 500 4106
SspI AAT'ATT
Cuts at: 711 711
Size: 4606
StuI AGG'CCT
Cuts at: 1553 1553
Size: 4606
StyI C'CwwG_G
Cuts at: 125 1269 1738 2092 125
Size: 1144 469 354 2639
Fragments arranged by size:
2639 1144 469 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 573 889 2187 2320 2779 2990 573
Size: 316 1298 133 459 211 2189
Fragments arranged by size:
2189 1298 459 316 211 133
TatI w'GTAC_w
Cuts at: 1578 4121 1578
Size: 2543 2063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 1482 1514 4123 4155 127
Size: 32 1323 32 2609 32 578
Fragments arranged by size:
2609 1323 578 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2401 2401
Size: 4606
XbaI T'CTAG_A
Cuts at: 1635 1635
Size: 4606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 2099
Size: 4606
XmaI C'CCGG_G
Cuts at: 1624 1624
Size: 4606
XmnI GAAnn'nnTTC
Cuts at: 353 479 353
Size: 126 4480
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI Bpu10I
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NotI PacI PasI PpuMI PshAI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SrfI SwaI XhoI ZraI