Restriction map for
pF1KSDA0759
(Circular) (Six-base) MAPSORT of: pF1KSDA0759.seq Check: 795 from: 1 to: 5149
pF1KSDA0759 5149 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 1852 1852
Size: 5149
AccI GT'mk_AC
Cuts at: 2185 2185
Size: 5149
Acc65I G'GTAC_C
Cuts at: 1822 2163 1822
Size: 341 4808
AclI AA'CG_TT
Cuts at: 4989 4989
Size: 5149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 850 2057 2069 2972 3404 4153 4421 422
Size: 428 1207 12 903 432 749 268 1150
Fragments arranged by size:
1207 1150 903 749 432 428 268 12
AflIII A'CryG_T
Cuts at: 202 2499 3605 4582 202
Size: 2297 1106 977 769
Fragments arranged by size:
2297 1106 977 769
AgeI A'CCGG_T
Cuts at: 3537 3537
Size: 5149
AhdI GACnn_n'nnGTC
Cuts at: 450 450
Size: 5149
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 220 252 220
Size: 32 5117
AlwNI CAG_nnn'CTG
Cuts at: 828 894 1400 2452 4021 828
Size: 66 506 1052 1569 1956
Fragments arranged by size:
1956 1569 1052 506 66
ApaI G_GGCC'C
Cuts at: 280 365 280
Size: 85 5064
ApaLI G'TGCA_C
Cuts at: 3919 3919
Size: 5149
ApoI r'AATT_y
Cuts at: 776 979 1428 2151 4502 4758 776
Size: 203 449 723 2351 256 1167
Fragments arranged by size:
2351 1167 723 449 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 5149
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
AvaI C'yCGr_G
Cuts at: 272 2167 272
Size: 1895 3254
BamHI G'GATC_C
Cuts at: 2172 2172
Size: 5149
BanI G'GyrC_C
Cuts at: 625 1822 2163 2577 2825 2860 625
Size: 1197 341 414 248 35 2914
Fragments arranged by size:
2914 1197 414 341 248 35
BanII G_rGCy'C
Cuts at: 280 365 559 593 910 917 1478 2161 3191
Size: 85 194 34 317 7 561 683 1030
Cuts at: 3191 280
Size: 2238
Fragments arranged by size:
2238 1030 683 561 317 194 85 34
7
BbeI G_GCGC'C
Cuts at: 2581 2829 2581
Size: 248 4901
BbsI GAAGACnn'nnnn_
Cuts at: 1526 1526
Size: 5149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1102 1136 1102
Size: 34 5115
BciVI GTATCCnnnnn_n'
Cuts at: 3045 3808 5149 3045
Size: 763 1341 3045
Fragments arranged by size:
3045 1341 763
BclI T'GATC_A
Cuts at: 1977 2667 1977
Size: 690 4459
BfrBI ATG'CAT
Cuts at: 1786 1786
Size: 5149
BglI GCCn_nnn'nGGC
Cuts at: 1345 1345
Size: 5149
BglII A'GATC_T
Cuts at: 2662 4701 2662
Size: 2039 3110
BlpI GC'TnA_GC
Cuts at: 2259 2259
Size: 5149
Bme1580I G_kGCm'C
Cuts at: 280 365 630 698 2772 2865 3923 280
Size: 85 265 68 2074 93 1058 1506
Fragments arranged by size:
2074 1506 1058 265 93 85 68
BmrI ACTGGGnnnn_n'
Cuts at: 588 613 999 1047 1109 2359 2440 2775 4921
Size: 25 386 48 62 1250 81 335 2146
Cuts at: 4921 588
Size: 816
Fragments arranged by size:
2146 1250 816 386 335 81 62 48
25
BmtI G_CTAG'C
Cuts at: 1166 2107 1166
Size: 941 4208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 819 3514 819
Size: 2695 2454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 556 693 997 1462 1794 440
Size: 116 137 304 465 332 3795
Fragments arranged by size:
3795 465 332 304 137 116
Bpu10I CC'TnA_GC
Cuts at: 281 686 821 887 281
Size: 405 135 66 4543
Fragments arranged by size:
4543 405 135 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1795 1816 1990 2323 3202 3696 3994 1795
Size: 21 174 333 879 494 298 2950
Fragments arranged by size:
2950 879 494 333 298 174 21
BsaI GGTCTCn'nnnn_
Cuts at: 4853 4853
Size: 5149
BsaAI yAC'GTr
Cuts at: 2139 2500 2139
Size: 361 4788
BsaBI GATnn'nnATC
Cuts at: 6 2661 4700 6
Size: 2655 2039 455
Fragments arranged by size:
2655 2039 455
BsaHI Gr'CG_yC
Cuts at: 1849 2578 2826 3530 5026 1849
Size: 729 248 704 1496 1972
Fragments arranged by size:
1972 1496 729 704 248
BsaWI w'CCGG_w
Cuts at: 2336 2857 3537 3811 3958 4369 2336
Size: 521 680 274 147 411 3116
Fragments arranged by size:
3116 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 580 610 580
Size: 30 5119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 1107 538
Size: 569 4580
BseYI C'CCAG_C
Cuts at: 657 809 1570 1874 2572 3330 3909 4405 657
Size: 152 761 304 698 758 579 496 1401
Fragments arranged by size:
1401 761 758 698 579 496 304 152
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3945 104
Size: 2585 46 1210 1308
Fragments arranged by size:
2585 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 344 910 917 1478 2161 2939 3129 3923 344
Size: 566 7 561 683 778 190 794 1570
Fragments arranged by size:
1570 794 778 683 566 561 190 7
BsmI GAATG_Cn'
Cuts at: 435 435
Size: 5149
BsmBI CGTCTCn'nnnn_
Cuts at: 1629 2026 1629
Size: 397 4752
Bsp1286I G_dGCh'C
Cuts at: 280 344 365 559 593 630 698 910 917
Size: 64 21 194 34 37 68 212 7
Cuts at: 917 1478 2161 2772 2865 2939 3129 3191 3923
Size: 561 683 611 93 74 190 62 732
Cuts at: 3923 280
Size: 1506
Fragments arranged by size:
1506 732 683 611 561 212 194 190
93 74 68 64 62 37 34 21
7
BspEI T'CCGG_A
Cuts at: 4369 4369
Size: 5149
BspHI T'CATG_A
Cuts at: 771 4325 771
Size: 3554 1595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 158 1758 2197 2411 2713 3094 158
Size: 1600 439 214 302 381 2213
Fragments arranged by size:
2213 1600 439 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3439 3493 4979 5144 50
Size: 3389 54 1486 165 55
Fragments arranged by size:
3389 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 924 3059 924
Size: 2135 3014
BsrFI r'CCGG_y
Cuts at: 159 3145 3326 3537 159
Size: 2986 181 211 1771
Fragments arranged by size:
2986 1771 211 181
BssSI C'ACGA_G
Cuts at: 3418 3778 3418
Size: 360 4789
BstAPI GCAn_nnn'nTGC
Cuts at: 607 1400 2524 607
Size: 793 1124 3232
Fragments arranged by size:
3232 1124 793
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5149
BstXI CCAn_nnnn'nTGG
Cuts at: 1356 1486 1578 1356
Size: 130 92 4927
Fragments arranged by size:
4927 130 92
BstYI r'GATC_y
Cuts at: 1 2172 2639 2662 2997 3243 4257 4343 4355
Size: 2171 467 23 335 246 1014 86 12
Cuts at: 4355 4701 1
Size: 346 449
Fragments arranged by size:
2171 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 263 1485 263
Size: 1222 3927
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1340 1668 3097 3278 3441 1340
Size: 328 1429 181 163 3048
Fragments arranged by size:
3048 1429 328 181 163
BtsI GCAGTG_nn'
Cuts at: 319 529 649 761 1398 1833 2390 319
Size: 210 120 112 637 435 557 3078
Fragments arranged by size:
3078 637 557 435 210 120 112
DraI TTT'AAA
Cuts at: 2146 2146
Size: 5149
DrdI GACnn_nn'nnGTC
Cuts at: 2853 3713 2853
Size: 860 4289
EaeI y'GGCC_r
Cuts at: 161 632 1346 1376 1647 1881 2732 2906 3297
Size: 471 714 30 271 234 851 174 391
Cuts at: 3297 3324 4671 161
Size: 27 1347 639
Fragments arranged by size:
1347 851 714 639 471 391 271 234
174 30 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5149
EarI CTCTTCn'nnn_
Cuts at: 178 419 644 698 2033 2088 3170 3380 178
Size: 241 225 54 1335 55 1082 210 1947
Fragments arranged by size:
1947 1335 1082 241 225 210 55 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1138 1375 3355 3667 3813 4959 1138
Size: 237 1980 312 146 1146 1328
Fragments arranged by size:
1980 1328 1146 312 237 146
EcoICRI GAG'CTC
Cuts at: 908 915 1476 2159 908
Size: 7 561 683 3898
Fragments arranged by size:
3898 683 561 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 440 556 693 850 997 1462 1794 2057
Size: 18 116 137 157 147 465 332 263
Cuts at: 2057 2069 2972 3404 4153 4421 422
Size: 12 903 432 749 268 1150
Fragments arranged by size:
1150 903 749 465 432 332 268 263
157 147 137 116 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 886 973 1809 886
Size: 87 836 4226
Fragments arranged by size:
4226 836 87
EcoO109I rG'GnC_Cy
Cuts at: 276 277 300 361 362 654 729 825 891
Size: 1 23 61 1 292 75 96 66
Cuts at: 891 993 1422 1557 2286 276
Size: 102 429 135 729 3139
Fragments arranged by size:
3139 729 429 292 135 102 96 75
66 61 23 1 1
EcoRI G'AATT_C
Cuts at: 979 2151 979
Size: 1172 3977
EcoRV GAT'ATC
Cuts at: 1002 1002
Size: 5149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3166 3166
Size: 5149
FspI TGC'GCA
Cuts at: 2928 2928
Size: 5149
HaeII r_GCGC'y
Cuts at: 2581 2829 3853 4832 2581
Size: 248 1024 979 2898
Fragments arranged by size:
2898 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 399 431 436 468 2989 3019 3021 3051 399
Size: 32 5 32 2521 30 2 30 2497
Fragments arranged by size:
2521 2497 32 32 30 30 5 2
HincII GTy'rAC
Cuts at: 2186 2186
Size: 5149
KasI G'GCGC_C
Cuts at: 2577 2825 2577
Size: 248 4901
KpnI G_GTAC'C
Cuts at: 1826 2167 1826
Size: 341 4808
MfeI C'AATT_G
Cuts at: 945 945
Size: 5149
MluI A'CGCG_T
Cuts at: 4582 4582
Size: 5149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 566 3820 4004 566
Size: 3254 184 1711
Fragments arranged by size:
3254 1711 184
MscI TGG'CCA
Cuts at: 634 1348 1378 2908 634
Size: 714 30 1530 2875
Fragments arranged by size:
2875 1530 714 30
MslI CAynn'nnrTG
Cuts at: 865 1900 3136 3263 865
Size: 1035 1236 127 2751
Fragments arranged by size:
2751 1236 1035 127
MspA1I CmG'CkG
Cuts at: 465 661 1047 1281 1948 2258 2572 2932 3947
Size: 196 386 234 667 310 314 360 1015
Cuts at: 3947 4192 465
Size: 245 1422
Fragments arranged by size:
1422 1015 667 386 360 314 310 245
234 196
NaeI GCC'GGC
Cuts at: 161 3328 161
Size: 3167 1982
NarI GG'CG_CC
Cuts at: 2578 2826 2578
Size: 248 4901
NcoI C'CATG_G
Cuts at: 263 1485 263
Size: 1222 3927
NgoMIV G'CCGG_C
Cuts at: 159 3326 159
Size: 3167 1982
NheI G'CTAG_C
Cuts at: 1162 2103 1162
Size: 941 4208
NruI TCG'CGA
Cuts at: 3510 3510
Size: 5149
NsiI A_TGCA'T
Cuts at: 1788 1788
Size: 5149
NspI r_CATG'y
Cuts at: 206 2200 2700 3609 206
Size: 1994 500 909 1746
Fragments arranged by size:
1994 1746 909 500
PasI CC'CwG_GG
Cuts at: 1578 1578
Size: 5149
PciI A'CATG_T
Cuts at: 202 3605 202
Size: 3403 1746
PfoI T'CCnGG_A
Cuts at: 417 1013 1022 1810 417
Size: 596 9 788 3756
Fragments arranged by size:
3756 788 596 9
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4288 4320 4288
Size: 32 5117
PpuMI rG'GwC_Cy
Cuts at: 654 891 993 1422 654
Size: 237 102 429 4381
Fragments arranged by size:
4381 429 237 102
PsiI TTA'TAA
Cuts at: 875 875
Size: 5149
PspOMI G'GGCC_C
Cuts at: 276 361 276
Size: 85 5064
PstI C_TGCA'G
Cuts at: 311 486 1556 1681 2194 2879 311
Size: 175 1070 125 513 685 2581
Fragments arranged by size:
2581 1070 685 513 175 125
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5149
PvuII CAG'CTG
Cuts at: 465 1047 1948 2572 2932 465
Size: 582 901 624 360 2682
Fragments arranged by size:
2682 901 624 582 360
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5149
SacI G_AGCT'C
Cuts at: 910 917 1478 2161 910
Size: 7 561 683 3898
Fragments arranged by size:
3898 683 561 7
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5149
SanDI GG'GwC_CC
Cuts at: 993 993
Size: 5149
SapI GCTCTTCn'nnn_
Cuts at: 2088 3170 3380 2088
Size: 1082 210 3857
Fragments arranged by size:
3857 1082 210
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5149
ScaI AGT'ACT
Cuts at: 1177 4666 1177
Size: 3489 1660
SfcI C'TryA_G
Cuts at: 33 307 482 1552 1677 1817 2190 2875 3870
Size: 274 175 1070 125 140 373 685 995
Cuts at: 3870 4061 33
Size: 191 1121
Fragments arranged by size:
1121 1070 995 685 373 274 191 175
140 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 1345 1345
Size: 5149
SfoI GGC'GCC
Cuts at: 2579 2827 2579
Size: 248 4901
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5149
SmlI C'TyrA_G
Cuts at: 1774 1795 2005 2302 3217 3711 3973 1774
Size: 21 210 297 915 494 262 2950
Fragments arranged by size:
2950 915 494 297 262 210 21
SnaBI TAC'GTA
Cuts at: 2139 2139
Size: 5149
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5149
SphI G_CATG'C
Cuts at: 2200 2700 2200
Size: 500 4649
StuI AGG'CCT
Cuts at: 1256 1256
Size: 5149
StyI C'CwwG_G
Cuts at: 263 1485 2281 2635 263
Size: 1222 796 354 2777
Fragments arranged by size:
2777 1222 796 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 613 823 1091 2730 2863 3322 3533 613
Size: 210 268 1639 133 459 211 2229
Fragments arranged by size:
2229 1639 459 268 211 210 133
TatI w'GTAC_w
Cuts at: 1175 4664 1175
Size: 3489 1660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4666 4698 4666
Size: 32 5117
Tth111I GACn'n_nGTC
Cuts at: 2944 2944
Size: 5149
XbaI T'CTAG_A
Cuts at: 2178 2178
Size: 5149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2115 2642 2115
Size: 527 4622
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5149
ZraI GAC'GTC
Cuts at: 1850 1850
Size: 5149
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NsiI NspI PasI
PciI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AscI AvrII BaeI
BbvCI BmgBI BsgI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII FseI FspAI HindIII HpaI
NdeI NotI PacI PflMI PmlI PshAI PspXI PsrI
SacII SexAI SgrAI SrfI SspI SwaI XhoI XmnI