Restriction map for
pF1KSDA0669
(Circular) (Six-base) MAPSORT of: pF1KSDA0669.seq Check: 4613 from: 1 to: 5479
pF1KSDA0669 5479 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AatII G_ACGT'C
Cuts at: 257 278 257
Size: 21 5458
AccI GT'mk_AC
Cuts at: 1690 1839 1898 2515 1690
Size: 149 59 617 4654
Fragments arranged by size:
4654 617 149 59
Acc65I G'GTAC_C
Cuts at: 2493 2493
Size: 5479
AclI AA'CG_TT
Cuts at: 5319 5319
Size: 5479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1993 3302 3734 4483 4751 1993
Size: 1309 432 749 268 2721
Fragments arranged by size:
2721 1309 749 432 268
AflIII A'CryG_T
Cuts at: 595 667 2123 2829 3935 4912 595
Size: 72 1456 706 1106 977 1162
Fragments arranged by size:
1456 1162 1106 977 706 72
AgeI A'CCGG_T
Cuts at: 3867 3867
Size: 5479
AlwNI CAG_nnn'CTG
Cuts at: 568 1095 2782 4351 568
Size: 527 1687 1569 1696
Fragments arranged by size:
1696 1687 1569 527
ApaI G_GGCC'C
Cuts at: 839 1082 1156 839
Size: 243 74 5162
Fragments arranged by size:
5162 243 74
ApaLI G'TGCA_C
Cuts at: 4249 4249
Size: 5479
ApoI r'AATT_y
Cuts at: 2481 4832 5088 2481
Size: 2351 256 2872
Fragments arranged by size:
2872 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5479
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5479
AvaI C'yCGr_G
Cuts at: 281 367 838 1011 1081 2497 281
Size: 86 471 173 70 1416 3263
Fragments arranged by size:
3263 1416 471 173 86 70
BamHI G'GATC_C
Cuts at: 2502 2502
Size: 5479
BanI G'GyrC_C
Cuts at: 781 1165 1324 1366 1632 2493 2907 3155 3190
Size: 384 159 42 266 861 414 248 35
Cuts at: 3190 781
Size: 3070
Fragments arranged by size:
3070 861 414 384 266 248 159 42
35
BanII G_rGCy'C
Cuts at: 550 647 839 1082 1156 1339 2491 3521 550
Size: 97 192 243 74 183 1152 1030 2508
Fragments arranged by size:
2508 1152 1030 243 192 183 97 74
BbeI G_GCGC'C
Cuts at: 785 1169 1328 2911 3159 785
Size: 384 159 1583 248 3105
Fragments arranged by size:
3105 1583 384 248 159
BbsI GAAGACnn'nnnn_
Cuts at: 1406 2084 1406
Size: 678 4801
BbvCI CC'TCA_GC
Cuts at: 1205 2460 1205
Size: 1255 4224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1476 1510 1476
Size: 34 5445
BciVI GTATCCnnnnn_n'
Cuts at: 1211 3375 4138 5479 1211
Size: 2164 763 1341 1211
Fragments arranged by size:
2164 1341 1211 763
BclI T'GATC_A
Cuts at: 617 2343 2997 617
Size: 1726 654 3099
Fragments arranged by size:
3099 1726 654
BglI GCCn_nnn'nGGC
Cuts at: 190 505 1174 1348 190
Size: 315 669 174 4321
Fragments arranged by size:
4321 669 315 174
BglII A'GATC_T
Cuts at: 2992 5031 2992
Size: 2039 3440
BlpI GC'TnA_GC
Cuts at: 1747 2589 1747
Size: 842 4637
Bme1580I G_kGCm'C
Cuts at: 839 1082 1156 1369 1499 1637 1667 3102 3195
Size: 243 74 213 130 138 30 1435 93
Cuts at: 3195 4253 839
Size: 1058 2065
Fragments arranged by size:
2065 1435 1058 243 213 138 130 93
74 30
BmgBI CAC'GTC
Cuts at: 668 668
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 1554 1728 2689 2770 3105 5251 1554
Size: 174 961 81 335 2146 1782
Fragments arranged by size:
2146 1782 961 335 174 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 273 339 3844 273
Size: 66 3505 1908
Fragments arranged by size:
3505 1908 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1511
Size: 5479
Bpu10I CC'TnA_GC
Cuts at: 1205 1806 1855 2460 1205
Size: 601 49 605 4224
Fragments arranged by size:
4224 605 601 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2653 3532 4026 4324 2653
Size: 879 494 298 3808
Fragments arranged by size:
3808 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5183 5183
Size: 5479
BsaAI yAC'GTr
Cuts at: 1278 2469 2830 1278
Size: 1191 361 3927
Fragments arranged by size:
3927 1191 361
BsaBI GATnn'nnATC
Cuts at: 6 2991 5030 6
Size: 2985 2039 455
Fragments arranged by size:
2985 2039 455
BsaHI Gr'CG_yC
Cuts at: 254 275 782 1166 1325 2908 3156 3860 5356
Size: 21 507 384 159 1583 248 704 1496
Cuts at: 5356 254
Size: 377
Fragments arranged by size:
1583 1496 704 507 384 377 248 159
21
BsaWI w'CCGG_w
Cuts at: 787 2666 3187 3867 4141 4288 4699 787
Size: 1879 521 680 274 147 411 1567
Fragments arranged by size:
1879 1567 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 244 274 428 431 458 461 782 812 1505
Size: 30 154 3 27 3 321 30 693
Cuts at: 1505 1535 2159 2189 2421 2451 244
Size: 30 624 30 232 30 3272
Fragments arranged by size:
3272 693 624 321 232 154 30 30
30 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 249 319 379 452 455 2448 249
Size: 70 60 73 3 1993 3280
Fragments arranged by size:
3280 1993 73 70 60 3
BseYI C'CCAG_C
Cuts at: 536 917 1057 1187 1418 1423 1549 1877 2902
Size: 381 140 130 231 5 126 328 1025
Cuts at: 2902 3660 4239 4735 536
Size: 758 579 496 1280
Fragments arranged by size:
1280 1025 758 579 496 381 328 231
140 130 126 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 1543
Size: 5479
BsiEI CG_ry'CG
Cuts at: 104 660 1025 3019 3065 4275 104
Size: 556 365 1994 46 1210 1308
Fragments arranged by size:
1994 1308 1210 556 365 46
BsiHKAI G_wGCw'C
Cuts at: 525 2491 3269 3459 4253 525
Size: 1966 778 190 794 1751
Fragments arranged by size:
1966 1751 794 778 190
BsmI GAATG_Cn'
Cuts at: 1383 1383
Size: 5479
BsmBI CGTCTCn'nnnn_
Cuts at: 261 282 328 381 261
Size: 21 46 53 5359
Fragments arranged by size:
5359 53 46 21
Bsp1286I G_dGCh'C
Cuts at: 525 550 647 839 1082 1156 1339 1369 1499
Size: 25 97 192 243 74 183 30 130
Cuts at: 1499 1637 1667 2491 3102 3195 3269 3459 3521
Size: 138 30 824 611 93 74 190 62
Cuts at: 3521 4253 525
Size: 732 1751
Fragments arranged by size:
1751 824 732 611 243 192 190 183
138 130 97 93 74 74 62 30
30 25
BspEI T'CCGG_A
Cuts at: 4699 4699
Size: 5479
BspHI T'CATG_A
Cuts at: 4655 4655
Size: 5479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1274 1458 2527 2741 3043 3424 1274
Size: 184 1069 214 302 381 3329
Fragments arranged by size:
3329 1069 381 302 214 184
BsrBI CCG'CTC
Cuts at: 50 638 765 1075 1669 3769 3823 5309 5474
Size: 588 127 310 594 2100 54 1486 165
Cuts at: 5474 50
Size: 55
Fragments arranged by size:
2100 1486 594 588 310 165 127 55
54
BsrDI GCAATG_nn'
Cuts at: 1949 2210 2346 3389 1949
Size: 261 136 1043 4039
Fragments arranged by size:
4039 1043 261 136
BsrFI r'CCGG_y
Cuts at: 138 1125 1282 1321 1369 3475 3656 3867 138
Size: 987 157 39 48 2106 181 211 1750
Fragments arranged by size:
2106 1750 987 211 181 157 48 39
BssHII G'CGCG_C
Cuts at: 1394 1394
Size: 5479
BssSI C'ACGA_G
Cuts at: 3748 4108 3748
Size: 360 5119
BstAPI GCAn_nnn'nTGC
Cuts at: 2854 2854
Size: 5479
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5479
BstXI CCAn_nnnn'nTGG
Cuts at: 486 1795 486
Size: 1309 4170
BstYI r'GATC_y
Cuts at: 1 715 2208 2502 2969 2992 3327 3573 4587
Size: 714 1493 294 467 23 335 246 1014
Cuts at: 4587 4673 4685 5031 1
Size: 86 12 346 449
Fragments arranged by size:
1493 1014 714 467 449 346 335 294
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 302 302
Size: 5479
BtgI C'CryG_G
Cuts at: 179 287 630 1172 1343 1361 1433 1628 179
Size: 108 343 542 171 18 72 195 4030
Fragments arranged by size:
4030 542 343 195 171 108 72 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3427 3608 3771 3427
Size: 181 163 5135
Fragments arranged by size:
5135 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1596 2411 2720 1166
Size: 430 815 309 3925
Fragments arranged by size:
3925 815 430 309
DraI TTT'AAA
Cuts at: 2261 2476 2261
Size: 215 5264
DraIII CAC_nnn'GTG
Cuts at: 1629 1629
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 703 870 3183 4043 703
Size: 167 2313 860 2139
Fragments arranged by size:
2313 2139 860 167
EaeI y'GGCC_r
Cuts at: 140 191 657 1251 1280 1349 1371 2033 3062
Size: 51 466 594 29 69 22 662 1029
Cuts at: 3062 3236 3627 3654 5001 140
Size: 174 391 27 1347 618
Fragments arranged by size:
1347 1029 662 618 594 466 391 174
69 51 29 27 22
EagI C'GGCC_G
Cuts at: 657 3062 657
Size: 2405 3074
EarI CTCTTCn'nnn_
Cuts at: 145 326 330 3500 3710 145
Size: 181 4 3170 210 1914
Fragments arranged by size:
3170 1914 210 181 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 543 1562 3685 3997 4143 5289 543
Size: 1019 2123 312 146 1146 733
Fragments arranged by size:
2123 1146 1019 733 312 146
EcoICRI GAG'CTC
Cuts at: 2489 2489
Size: 5479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1993 3302 3734 4483 4751 1511
Size: 482 1309 432 749 268 2239
Fragments arranged by size:
2239 1309 749 482 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1078 1153 2616 1078
Size: 75 1463 3941
Fragments arranged by size:
3941 1463 75
EcoRI G'AATT_C
Cuts at: 2481 2481
Size: 5479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 174 355 2312 3496 174
Size: 181 1957 1184 2157
Fragments arranged by size:
2157 1957 1184 181
FseI GG_CCGG'CC
Cuts at: 1286 1286
Size: 5479
FspI TGC'GCA
Cuts at: 3258 3258
Size: 5479
HaeII r_GCGC'y
Cuts at: 475 785 1169 1328 1813 2911 3159 4183 5162
Size: 310 384 159 485 1098 248 1024 979
Cuts at: 5162 475
Size: 792
Fragments arranged by size:
1098 1024 979 792 485 384 310 248
159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 428 460 2143 2175 2335 2367 3319
Size: 32 152 32 1683 32 160 32 952
Cuts at: 3319 3349 3351 3381 244
Size: 30 2 30 2342
Fragments arranged by size:
2342 1683 952 160 152 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1899 2015 2516 1899
Size: 116 501 4862
Fragments arranged by size:
4862 501 116
HpaI GTT'AAC
Cuts at: 2015 2015
Size: 5479
KasI G'GCGC_C
Cuts at: 781 1165 1324 2907 3155 781
Size: 384 159 1583 248 3105
Fragments arranged by size:
3105 1583 384 248 159
KpnI G_GTAC'C
Cuts at: 2497 2497
Size: 5479
MluI A'CGCG_T
Cuts at: 4912 4912
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 869 4150 4334 869
Size: 3281 184 2014
Fragments arranged by size:
3281 2014 184
MscI TGG'CCA
Cuts at: 193 2035 3238 193
Size: 1842 1203 2434
Fragments arranged by size:
2434 1842 1203
MslI CAynn'nnrTG
Cuts at: 3466 3593 3466
Size: 127 5352
MspA1I CmG'CkG
Cuts at: 405 412 773 999 1174 1599 1746 2588 2902
Size: 7 361 226 175 425 147 842 314
Cuts at: 2902 3262 4277 4522 405
Size: 360 1015 245 1362
Fragments arranged by size:
1362 1015 842 425 361 360 314 245
226 175 147 7
NaeI GCC'GGC
Cuts at: 140 1127 1284 3658 140
Size: 987 157 2374 1961
Fragments arranged by size:
2374 1961 987 157
NarI GG'CG_CC
Cuts at: 782 1166 1325 2908 3156 782
Size: 384 159 1583 248 3105
Fragments arranged by size:
3105 1583 384 248 159
NcoI C'CATG_G
Cuts at: 1433 1433
Size: 5479
NgoMIV G'CCGG_C
Cuts at: 138 1125 1282 3656 138
Size: 987 157 2374 1961
Fragments arranged by size:
2374 1961 987 157
NruI TCG'CGA
Cuts at: 3840 3840
Size: 5479
NspI r_CATG'y
Cuts at: 599 1795 2530 3030 3939 599
Size: 1196 735 500 909 2139
Fragments arranged by size:
2139 1196 909 735 500
PasI CC'CwG_GG
Cuts at: 1068 1068
Size: 5479
PciI A'CATG_T
Cuts at: 595 3935 595
Size: 3340 2139
PmeI GTTT'AAAC
Cuts at: 2476 2476
Size: 5479
PmlI CAC'GTG
Cuts at: 1278 1278
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4618 4650 4618
Size: 32 5447
PspOMI G'GGCC_C
Cuts at: 835 1078 1152 835
Size: 243 74 5162
Fragments arranged by size:
5162 243 74
PstI C_TGCA'G
Cuts at: 573 1161 1271 1292 1659 2524 3209 573
Size: 588 110 21 367 865 685 2843
Fragments arranged by size:
2843 865 685 588 367 110 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5479
PvuII CAG'CTG
Cuts at: 405 1746 2902 3262 405
Size: 1341 1156 360 2622
Fragments arranged by size:
2622 1341 1156 360
RsrII CG'GwC_CG
Cuts at: 3672 3672
Size: 5479
SacI G_AGCT'C
Cuts at: 2491 2491
Size: 5479
SacII CC_GC'GG
Cuts at: 1175 1175
Size: 5479
SalI G'TCGA_C
Cuts at: 1897 2514 1897
Size: 617 4862
SapI GCTCTTCn'nnn_
Cuts at: 145 330 3500 3710 145
Size: 185 3170 210 1914
Fragments arranged by size:
3170 1914 210 185
SbfI CC_TGCA'GG
Cuts at: 2524 2524
Size: 5479
ScaI AGT'ACT
Cuts at: 744 4996 744
Size: 4252 1227
SexAI A'CCwGG_T
Cuts at: 1611 1611
Size: 5479
SfcI C'TryA_G
Cuts at: 33 569 648 1157 1267 1288 1655 2520 3205
Size: 536 79 509 110 21 367 865 685
Cuts at: 3205 4200 4391 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 865 685 536 509 367 191
110 79 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 190 190
Size: 5479
SfoI GGC'GCC
Cuts at: 783 1167 1326 2909 3157 783
Size: 384 159 1583 248 3105
Fragments arranged by size:
3105 1583 384 248 159
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1321 1321
Size: 5479
SmaI CCC'GGG
Cuts at: 369 1013 1083 2499 369
Size: 644 70 1416 3349
Fragments arranged by size:
3349 1416 644 70
SmlI C'TyrA_G
Cuts at: 2632 3547 4041 4303 2632
Size: 915 494 262 3808
Fragments arranged by size:
3808 915 494 262
SnaBI TAC'GTA
Cuts at: 2469 2469
Size: 5479
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5479
SphI G_CATG'C
Cuts at: 2530 3030 2530
Size: 500 4979
StyI C'CwwG_G
Cuts at: 1433 2611 2965 1433
Size: 1178 354 3947
Fragments arranged by size:
3947 1178 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1525 1573 3060 3193 3652 3863 1525
Size: 48 1487 133 459 211 3141
Fragments arranged by size:
3141 1487 459 211 133 48
TatI w'GTAC_w
Cuts at: 742 4994 742
Size: 4252 1227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 782 814 1505 1537 4996 5028 782
Size: 32 691 32 3459 32 1233
Fragments arranged by size:
3459 1233 691 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3274 3274
Size: 5479
XbaI T'CTAG_A
Cuts at: 2508 2508
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 186 1359 1431 2972 186
Size: 1173 72 1541 2693
Fragments arranged by size:
2693 1541 1173 72
XmaI C'CCGG_G
Cuts at: 367 1011 1081 2497 367
Size: 644 70 1416 3349
Fragments arranged by size:
3349 1416 644 70
ZraI GAC'GTC
Cuts at: 255 276 255
Size: 21 5458
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmtI BsiWI BsrGI BstEII BstZ17I
ClaI CspCI EcoNI EcoRV FspAI HindIII MfeI NdeI
NheI NotI NsiI PacI PflMI PfoI PpuMI PshAI
PsiI PspXI PsrI SanDI SrfI SspI StuI SwaI
XhoI XmnI