Restriction map for
pF1KSDA0668
(Circular) (Six-base) MAPSORT of: pF1KSDA0668.seq Check: 6841 from: 1 to: 5290
pF1KSDA0668 5290 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1695 2245 1695
Size: 550 4740
AatII G_ACGT'C
Cuts at: 725 725
Size: 5290
AccI GT'mk_AC
Cuts at: 2326 2326
Size: 5290
Acc65I G'GTAC_C
Cuts at: 689 1871 2304 689
Size: 1182 433 3675
Fragments arranged by size:
3675 1182 433
AclI AA'CG_TT
Cuts at: 5130 5130
Size: 5290
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 775 1174 1318 1435 1537 2235 3113 3545
Size: 136 399 144 117 102 698 878 432
Cuts at: 3545 4294 4562 639
Size: 749 268 1367
Fragments arranged by size:
1367 878 749 698 432 399 268 144
136 117 102
AfeI AGC'GCT
Cuts at: 1738 1738
Size: 5290
AflIII A'CryG_T
Cuts at: 2640 3746 4723 2640
Size: 1106 977 3207
Fragments arranged by size:
3207 1106 977
AgeI A'CCGG_T
Cuts at: 3678 3678
Size: 5290
AleI CACnn'nnGTG
Cuts at: 1609 1609
Size: 5290
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1477 1509 1477
Size: 32 5258
AlwNI CAG_nnn'CTG
Cuts at: 1296 1398 1731 2593 4162 1296
Size: 102 333 862 1569 2424
Fragments arranged by size:
2424 1569 862 333 102
ApaI G_GGCC'C
Cuts at: 307 307
Size: 5290
ApaLI G'TGCA_C
Cuts at: 1708 1912 4060 1708
Size: 204 2148 2938
Fragments arranged by size:
2938 2148 204
ApoI r'AATT_y
Cuts at: 1003 2292 4643 4899 1003
Size: 1289 2351 256 1394
Fragments arranged by size:
2351 1394 1289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5290
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5290
AvaI C'yCGr_G
Cuts at: 519 959 1523 1889 2227 2308 519
Size: 440 564 366 338 81 3501
Fragments arranged by size:
3501 564 440 366 338 81
BamHI G'GATC_C
Cuts at: 2204 2313 2204
Size: 109 5181
BanI G'GyrC_C
Cuts at: 679 689 1871 2304 2718 2966 3001 679
Size: 10 1182 433 414 248 35 2968
Fragments arranged by size:
2968 1182 433 414 248 35 10
BanII G_rGCy'C
Cuts at: 202 307 379 514 616 1068 1345 1426 1550
Size: 105 72 135 102 452 277 81 124
Cuts at: 1550 2270 2302 3332 202
Size: 720 32 1030 2160
Fragments arranged by size:
2160 1030 720 452 277 135 124 105
102 81 72 32
BbeI G_GCGC'C
Cuts at: 2722 2970 2722
Size: 248 5042
BbsI GAAGACnn'nnnn_
Cuts at: 717 763 1244 2090 717
Size: 46 481 846 3917
Fragments arranged by size:
3917 846 481 46
BbvCI CC'TCA_GC
Cuts at: 1357 1846 1357
Size: 489 4801
BciVI GTATCCnnnnn_n'
Cuts at: 3186 3949 5290 3186
Size: 763 1341 3186
Fragments arranged by size:
3186 1341 763
BclI T'GATC_A
Cuts at: 2808 2808
Size: 5290
BglI GCCn_nnn'nGGC
Cuts at: 648 1856 648
Size: 1208 4082
BglII A'GATC_T
Cuts at: 2803 4842 2803
Size: 2039 3251
BlpI GC'TnA_GC
Cuts at: 941 1574 2400 941
Size: 633 826 3831
Fragments arranged by size:
3831 826 633
Bme1580I G_kGCm'C
Cuts at: 307 1712 1916 2015 2052 2913 3006 4064 307
Size: 1405 204 99 37 861 93 1058 1533
Fragments arranged by size:
1533 1405 1058 861 204 99 93 37
BmgBI CAC'GTC
Cuts at: 797 1482 797
Size: 685 4605
BmrI ACTGGGnnnn_n'
Cuts at: 417 1875 2226 2500 2581 2916 5062 417
Size: 1458 351 274 81 335 2146 645
Fragments arranged by size:
2146 1458 645 351 335 274 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1915 3655 1915
Size: 1740 3550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 994 1069 1141 1387 1609 1798 1885 2028
Size: 591 75 72 246 222 189 87 143
Cuts at: 2028 403
Size: 3665
Fragments arranged by size:
3665 591 246 222 189 143 87 75
72
Bpu10I CC'TnA_GC
Cuts at: 1093 1357 1657 1846 1093
Size: 264 300 189 4537
Fragments arranged by size:
4537 300 264 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2464 3343 3837 4135 2464
Size: 879 494 298 3619
Fragments arranged by size:
3619 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1813 1822 4994 1813
Size: 9 3172 2109
Fragments arranged by size:
3172 2109 9
BsaAI yAC'GTr
Cuts at: 546 2183 2280 2641 546
Size: 1637 97 361 3195
Fragments arranged by size:
3195 1637 361 97
BsaBI GATnn'nnATC
Cuts at: 6 2802 4841 6
Size: 2796 2039 455
Fragments arranged by size:
2796 2039 455
BsaHI Gr'CG_yC
Cuts at: 401 722 981 2719 2967 3671 5167 401
Size: 321 259 1738 248 704 1496 524
Fragments arranged by size:
1738 1496 704 524 321 259 248
BsaWI w'CCGG_w
Cuts at: 2477 2998 3678 3952 4099 4510 2477
Size: 521 680 274 147 411 3257
Fragments arranged by size:
3257 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 447 450 519 1576 1714 1834 447
Size: 3 69 1057 138 120 3903
Fragments arranged by size:
3903 1057 138 120 69 3
BseYI C'CCAG_C
Cuts at: 208 288 300 905 1454 1928 2713 3471 4050
Size: 80 12 605 549 474 785 758 579
Cuts at: 4050 4546 208
Size: 496 952
Fragments arranged by size:
952 785 758 605 579 549 496 474
80 12
BsiEI CG_ry'CG
Cuts at: 104 2830 2876 4086 104
Size: 2726 46 1210 1308
Fragments arranged by size:
2726 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 202 223 379 1345 1426 1712 1916 2302 3080
Size: 21 156 966 81 286 204 386 778
Cuts at: 3080 3270 4064 202
Size: 190 794 1428
Fragments arranged by size:
1428 966 794 778 386 286 204 190
156 81 21
BsmBI CGTCTCn'nnnn_
Cuts at: 606 606
Size: 5290
Bsp1286I G_dGCh'C
Cuts at: 202 223 307 379 514 616 1068 1345 1426
Size: 21 84 72 135 102 452 277 81
Cuts at: 1426 1550 1712 1916 2015 2052 2270 2302 2913
Size: 124 162 204 99 37 218 32 611
Cuts at: 2913 3006 3080 3270 3332 4064 202
Size: 93 74 190 62 732 1428
Fragments arranged by size:
1428 732 611 452 277 218 204 190
162 135 124 102 99 93 84 81
74 72 62 37 32 21
BspEI T'CCGG_A
Cuts at: 4510 4510
Size: 5290
BspHI T'CATG_A
Cuts at: 4466 4466
Size: 5290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 428 1695 2096 2245 2338 2552 2854 3235 428
Size: 1267 401 149 93 214 302 381 2483
Fragments arranged by size:
2483 1267 401 381 302 214 149 93
BsrBI CCG'CTC
Cuts at: 50 978 3580 3634 5120 5285 50
Size: 928 2602 54 1486 165 55
Fragments arranged by size:
2602 1486 928 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3200 3200
Size: 5290
BsrFI r'CCGG_y
Cuts at: 647 1381 3286 3467 3678 647
Size: 734 1905 181 211 2259
Fragments arranged by size:
2259 1905 734 211 181
BssSI C'ACGA_G
Cuts at: 352 1099 3559 3919 352
Size: 747 2460 360 1723
Fragments arranged by size:
2460 1723 747 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1731 2665 1731
Size: 934 4356
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5290
BstXI CCAn_nnnn'nTGG
Cuts at: 1038 1081 1038
Size: 43 5247
BstYI r'GATC_y
Cuts at: 1 1460 1598 2204 2313 2780 2803 3138 3384
Size: 1459 138 606 109 467 23 335 246
Cuts at: 3384 4398 4484 4496 4842 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1459 1014 606 467 449 346 335 246
138 109 86 23 12
BtgI C'CryG_G
Cuts at: 1646 1958 1646
Size: 312 4978
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 908 3238 3419 3582 908
Size: 2330 181 163 2616
Fragments arranged by size:
2616 2330 181 163
BtsI GCAGTG_nn'
Cuts at: 2531 2531
Size: 5290
DraI TTT'AAA
Cuts at: 231 2287 231
Size: 2056 3234
DraIII CAC_nnn'GTG
Cuts at: 1719 1719
Size: 5290
DrdI GACnn_nn'nnGTC
Cuts at: 1326 2994 3854 1326
Size: 1668 860 2762
Fragments arranged by size:
2762 1668 860
EaeI y'GGCC_r
Cuts at: 632 1071 1379 1634 2177 2873 3047 3438 3465
Size: 439 308 255 543 696 174 391 27
Cuts at: 3465 4812 632
Size: 1347 1110
Fragments arranged by size:
1347 1110 696 543 439 391 308 255
174 27
EagI C'GGCC_G
Cuts at: 2873 2873
Size: 5290
EarI CTCTTCn'nnn_
Cuts at: 250 519 658 1249 1858 3311 3521 250
Size: 269 139 591 609 1453 210 2019
Fragments arranged by size:
2019 1453 609 591 269 210 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1447 1954 1972 3496 3808 3954 5100 1447
Size: 507 18 1524 312 146 1146 1637
Fragments arranged by size:
1637 1524 1146 507 312 146 18
EcoICRI GAG'CTC
Cuts at: 200 377 1343 1424 2300 200
Size: 177 966 81 876 3190
Fragments arranged by size:
3190 966 876 177 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 639 775 994 1069 1141 1174 1318 1387
Size: 236 136 219 75 72 33 144 69
Cuts at: 1387 1435 1537 1609 1798 1885 2028 2235 3113
Size: 48 102 72 189 87 143 207 878
Cuts at: 3113 3545 4294 4562 403
Size: 432 749 268 1131
Fragments arranged by size:
1131 878 749 432 268 236 219 207
189 144 143 136 102 87 75 72
72 69 48 33
EcoNI CCTnn'n_nnAGG
Cuts at: 255 1156 1390 2092 255
Size: 901 234 702 3453
Fragments arranged by size:
3453 901 702 234
EcoO109I rG'GnC_Cy
Cuts at: 248 417 1728 2166 2427 248
Size: 169 1311 438 261 3111
Fragments arranged by size:
3111 1311 438 261 169
EcoRI G'AATT_C
Cuts at: 2292 2292
Size: 5290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 5290
FseI GG_CCGG'CC
Cuts at: 1385 1385
Size: 5290
FspI TGC'GCA
Cuts at: 3069 3069
Size: 5290
HaeII r_GCGC'y
Cuts at: 147 282 295 599 744 1117 1740 2722 2970
Size: 135 13 304 145 373 623 982 248
Cuts at: 2970 3994 4973 147
Size: 1024 979 464
Fragments arranged by size:
1024 982 979 623 464 373 304 248
145 135 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1471 1503 1557 1589 3130 3160 3162 3192 1471
Size: 32 54 32 1541 30 2 30 3569
Fragments arranged by size:
3569 1541 54 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2327 2327
Size: 5290
KasI G'GCGC_C
Cuts at: 2718 2966 2718
Size: 248 5042
KpnI G_GTAC'C
Cuts at: 693 1875 2308 693
Size: 1182 433 3675
Fragments arranged by size:
3675 1182 433
MluI A'CGCG_T
Cuts at: 4723 4723
Size: 5290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1037 3961 4145 1037
Size: 2924 184 2182
Fragments arranged by size:
2924 2182 184
MscI TGG'CCA
Cuts at: 634 1073 2179 3049 634
Size: 439 1106 870 2875
Fragments arranged by size:
2875 1106 870 439
MslI CAynn'nnrTG
Cuts at: 1609 3277 3404 1609
Size: 1668 127 3495
Fragments arranged by size:
3495 1668 127
MspA1I CmG'CkG
Cuts at: 191 300 473 706 783 1296 1377 1578 1648
Size: 109 173 233 77 513 81 201 70
Cuts at: 1648 2399 2713 3073 4088 4333 191
Size: 751 314 360 1015 245 1148
Fragments arranged by size:
1148 1015 751 513 360 314 245 233
201 173 109 81 77 70
NaeI GCC'GGC
Cuts at: 649 1383 3469 649
Size: 734 2086 2470
Fragments arranged by size:
2470 2086 734
NarI GG'CG_CC
Cuts at: 2719 2967 2719
Size: 248 5042
NgoMIV G'CCGG_C
Cuts at: 647 1381 3467 647
Size: 734 2086 2470
Fragments arranged by size:
2470 2086 734
NruI TCG'CGA
Cuts at: 3651 3651
Size: 5290
NspI r_CATG'y
Cuts at: 2011 2341 2841 3750 2011
Size: 330 500 909 3551
Fragments arranged by size:
3551 909 500 330
PasI CC'CwG_GG
Cuts at: 162 162
Size: 5290
PciI A'CATG_T
Cuts at: 3746 3746
Size: 5290
PflMI CCAn_nnn'nTGG
Cuts at: 2193 2193
Size: 5290
PfoI T'CCnGG_A
Cuts at: 1190 1334 1190
Size: 144 5146
PmeI GTTT'AAAC
Cuts at: 2287 2287
Size: 5290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 346 378 4429 4461 346
Size: 32 4051 32 1175
Fragments arranged by size:
4051 1175 32 32
PpuMI rG'GwC_Cy
Cuts at: 248 417 248
Size: 169 5121
PspOMI G'GGCC_C
Cuts at: 303 303
Size: 5290
PstI C_TGCA'G
Cuts at: 842 1391 1673 1736 2093 2335 3020 842
Size: 549 282 63 357 242 685 3112
Fragments arranged by size:
3112 685 549 357 282 242 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5290
PvuII CAG'CTG
Cuts at: 300 706 1296 1377 1578 2713 3073 300
Size: 406 590 81 201 1135 360 2517
Fragments arranged by size:
2517 1135 590 406 360 201 81
RsrII CG'GwC_CG
Cuts at: 3483 3483
Size: 5290
SacI G_AGCT'C
Cuts at: 202 379 1345 1426 2302 202
Size: 177 966 81 876 3190
Fragments arranged by size:
3190 966 876 177 81
SacII CC_GC'GG
Cuts at: 1649 1649
Size: 5290
SalI G'TCGA_C
Cuts at: 2325 2325
Size: 5290
SapI GCTCTTCn'nnn_
Cuts at: 519 658 3311 3521 519
Size: 139 2653 210 2288
Fragments arranged by size:
2653 2288 210 139
SbfI CC_TGCA'GG
Cuts at: 2335 2335
Size: 5290
ScaI AGT'ACT
Cuts at: 4807 4807
Size: 5290
SexAI A'CCwGG_T
Cuts at: 685 2186 685
Size: 1501 3789
SfcI C'TryA_G
Cuts at: 33 333 838 1387 1669 1732 1740 2089 2331
Size: 300 505 549 282 63 8 349 242
Cuts at: 2331 3016 4011 4202 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 685 549 505 349 300 282
242 191 63 8
SfoI GGC'GCC
Cuts at: 2720 2968 2720
Size: 248 5042
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5290
SmaI CCC'GGG
Cuts at: 961 2310 961
Size: 1349 3941
SmlI C'TyrA_G
Cuts at: 2443 3358 3852 4114 2443
Size: 915 494 262 3619
Fragments arranged by size:
3619 915 494 262
SnaBI TAC'GTA
Cuts at: 2280 2280
Size: 5290
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5290
SphI G_CATG'C
Cuts at: 2341 2841 2341
Size: 500 4790
StuI AGG'CCT
Cuts at: 2020 2020
Size: 5290
StyI C'CwwG_G
Cuts at: 2015 2054 2109 2422 2776 2015
Size: 39 55 313 354 4529
Fragments arranged by size:
4529 354 313 55 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 384 1621 1931 2043 2871 3004 3463 3674 384
Size: 1237 310 112 828 133 459 211 2000
Fragments arranged by size:
2000 1237 828 459 310 211 133 112
TatI w'GTAC_w
Cuts at: 2231 4805 2231
Size: 2574 2716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4807 4839 4807
Size: 32 5258
Tth111I GACn'n_nGTC
Cuts at: 3085 3085
Size: 5290
XbaI T'CTAG_A
Cuts at: 2319 2319
Size: 5290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 378 477 1081 1925 2187 2783 378
Size: 99 604 844 262 596 2885
Fragments arranged by size:
2885 844 604 596 262 99
XmaI C'CCGG_G
Cuts at: 959 2308 959
Size: 1349 3941
XmnI GAAnn'nnTTC
Cuts at: 760 760
Size: 5290
ZraI GAC'GTC
Cuts at: 723 723
Size: 5290
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AhdI AlfI AscI AvrII BaeI BcgI BfrBI
BmtI BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SrfI
SspI SwaI XhoI