Restriction map for
pF1KSDA0646
(Circular) (Six-base) MAPSORT of: pF1KSDA0646.seq Check: 7443 from: 1 to: 4594
pF1KSDA0646 4594 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1630 1630
Size: 4594
Acc65I G'GTAC_C
Cuts at: 1608 1608
Size: 4594
AclI AA'CG_TT
Cuts at: 4434 4434
Size: 4594
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 644 827 985 1081 1153 2417 2849 3598
Size: 123 183 158 96 72 1264 432 749
Cuts at: 3598 3866 521
Size: 268 1249
Fragments arranged by size:
1264 1249 749 432 268 183 158 123
96 72
AflIII A'CryG_T
Cuts at: 1944 3050 4027 1944
Size: 1106 977 2511
Fragments arranged by size:
2511 1106 977
AgeI A'CCGG_T
Cuts at: 2982 2982
Size: 4594
AhdI GACnn_n'nnGTC
Cuts at: 1011 1458 1011
Size: 447 4147
AlwNI CAG_nnn'CTG
Cuts at: 616 1155 1391 1897 3466 616
Size: 539 236 506 1569 1744
Fragments arranged by size:
1744 1569 539 506 236
ApaLI G'TGCA_C
Cuts at: 3364 3364
Size: 4594
ApoI r'AATT_y
Cuts at: 589 636 1596 3947 4203 589
Size: 47 960 2351 256 980
Fragments arranged by size:
2351 980 960 256 47
AseI AT'TA_AT
Cuts at: 20 1496 20
Size: 1476 3118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4594
AvaI C'yCGr_G
Cuts at: 1612 1612
Size: 4594
BamHI G'GATC_C
Cuts at: 735 1617 735
Size: 882 3712
BanI G'GyrC_C
Cuts at: 179 1608 2022 2270 2305 179
Size: 1429 414 248 35 2468
Fragments arranged by size:
2468 1429 414 248 35
BanII G_rGCy'C
Cuts at: 137 780 1173 1328 1522 1606 2636 137
Size: 643 393 155 194 84 1030 2095
Fragments arranged by size:
2095 1030 643 393 194 155 84
BbeI G_GCGC'C
Cuts at: 2026 2274 2026
Size: 248 4346
BbsI GAAGACnn'nnnn_
Cuts at: 512 1075 512
Size: 563 4031
BciVI GTATCCnnnnn_n'
Cuts at: 2490 3253 4594 2490
Size: 763 1341 2490
Fragments arranged by size:
2490 1341 763
BclI T'GATC_A
Cuts at: 2112 2112
Size: 4594
BglII A'GATC_T
Cuts at: 2107 4146 2107
Size: 2039 2555
BlpI GC'TnA_GC
Cuts at: 1037 1704 1037
Size: 667 3927
Bme1580I G_kGCm'C
Cuts at: 1383 2217 2310 3368 1383
Size: 834 93 1058 2609
Fragments arranged by size:
2609 1058 834 93
BmrI ACTGGGnnnn_n'
Cuts at: 519 1804 1885 2220 4366 519
Size: 1285 81 335 2146 747
Fragments arranged by size:
2146 1285 747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2959 2959
Size: 4594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 638
Size: 4594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1768 2647 3141 3439 775
Size: 993 879 494 298 1930
Fragments arranged by size:
1930 993 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 149 4298 149
Size: 4149 445
BsaAI yAC'GTr
Cuts at: 1584 1945 1584
Size: 361 4233
BsaBI GATnn'nnATC
Cuts at: 6 2106 4145 6
Size: 2100 2039 455
Fragments arranged by size:
2100 2039 455
BsaHI Gr'CG_yC
Cuts at: 2023 2271 2975 4471 2023
Size: 248 704 1496 2146
Fragments arranged by size:
2146 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1781 2302 2982 3256 3403 3814 1781
Size: 521 680 274 147 411 2561
Fragments arranged by size:
2561 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 762 792 762
Size: 30 4564
BseYI C'CCAG_C
Cuts at: 1016 2017 2775 3354 3850 1016
Size: 1001 758 579 496 1760
Fragments arranged by size:
1760 1001 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1046 1046
Size: 4594
BsiEI CG_ry'CG
Cuts at: 104 2134 2180 3390 104
Size: 2030 46 1210 1308
Fragments arranged by size:
2030 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1328 1522 1606 2384 2574 3368 1328
Size: 194 84 778 190 794 2554
Fragments arranged by size:
2554 794 778 194 190 84
BsmI GAATG_Cn'
Cuts at: 480 1438 480
Size: 958 3636
Bsp1286I G_dGCh'C
Cuts at: 137 780 1173 1328 1383 1522 1606 2217 2310
Size: 643 393 155 55 139 84 611 93
Cuts at: 2310 2384 2574 2636 3368 137
Size: 74 190 62 732 1363
Fragments arranged by size:
1363 732 643 611 393 190 155 139
93 84 74 62 55
BspEI T'CCGG_A
Cuts at: 3814 3814
Size: 4594
BspHI T'CATG_A
Cuts at: 819 3770 819
Size: 2951 1643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 601 1642 1856 2158 2539 601
Size: 1041 214 302 381 2656
Fragments arranged by size:
2656 1041 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2884 2938 4424 4589 50
Size: 2834 54 1486 165 55
Fragments arranged by size:
2834 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2504 2504
Size: 4594
BsrFI r'CCGG_y
Cuts at: 163 2590 2771 2982 163
Size: 2427 181 211 1775
Fragments arranged by size:
2427 1775 211 181
BssSI C'ACGA_G
Cuts at: 2863 3223 2863
Size: 360 4234
BstAPI GCAn_nnn'nTGC
Cuts at: 1969 1969
Size: 4594
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4594
BstEII G'GTnAC_C
Cuts at: 876 876
Size: 4594
BstYI r'GATC_y
Cuts at: 1 735 1617 2084 2107 2442 2688 3702 3788
Size: 734 882 467 23 335 246 1014 86
Cuts at: 3788 3800 4146 1
Size: 12 346 449
Fragments arranged by size:
1014 882 734 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 335 335
Size: 4594
BtgI C'CryG_G
Cuts at: 125 125
Size: 4594
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2542 2723 2886 2542
Size: 181 163 4250
Fragments arranged by size:
4250 181 163
BtsI GCAGTG_nn'
Cuts at: 1835 1835
Size: 4594
DraI TTT'AAA
Cuts at: 1591 1591
Size: 4594
DrdI GACnn_nn'nnGTC
Cuts at: 2298 3158 2298
Size: 860 3734
EaeI y'GGCC_r
Cuts at: 168 710 2177 2351 2742 2769 4116 168
Size: 542 1467 174 391 27 1347 646
Fragments arranged by size:
1467 1347 646 542 391 174 27
EagI C'GGCC_G
Cuts at: 2177 2177
Size: 4594
EarI CTCTTCn'nnn_
Cuts at: 846 1076 1175 1250 2615 2825 846
Size: 230 99 75 1365 210 2615
Fragments arranged by size:
2615 1365 230 210 99 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2800 3112 3258 4404 2800
Size: 312 146 1146 2990
Fragments arranged by size:
2990 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1326 1520 1604 1326
Size: 194 84 4316
Fragments arranged by size:
4316 194 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 638 644 827 985 1081 1153 2417 2849
Size: 117 6 183 158 96 72 1264 432
Cuts at: 2849 3598 3866 521
Size: 749 268 1249
Fragments arranged by size:
1264 1249 749 432 268 183 158 117
96 72 6
EcoO109I rG'GnC_Cy
Cuts at: 613 628 1128 1731 613
Size: 15 500 603 3476
Fragments arranged by size:
3476 603 500 15
EcoRI G'AATT_C
Cuts at: 1596 1596
Size: 4594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1221 1309 2611 1221
Size: 88 1302 3204
Fragments arranged by size:
3204 1302 88
FspI TGC'GCA
Cuts at: 2373 2373
Size: 4594
HaeII r_GCGC'y
Cuts at: 206 2026 2274 3298 4277 206
Size: 1820 248 1024 979 523
Fragments arranged by size:
1820 1024 979 523 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 245 277 288 320 425 457 507 539 997
Size: 32 11 32 105 32 50 32 458
Cuts at: 997 1029 1444 1476 2434 2464 2466 2496 245
Size: 32 415 32 958 30 2 30 2343
Fragments arranged by size:
2343 958 458 415 105 50 32 32
32 32 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 746 1631 746
Size: 885 3709
HindIII A'AGCT_T
Cuts at: 858 858
Size: 4594
KasI G'GCGC_C
Cuts at: 2022 2270 2022
Size: 248 4346
KpnI G_GTAC'C
Cuts at: 1612 1612
Size: 4594
MfeI C'AATT_G
Cuts at: 1488 1488
Size: 4594
MluI A'CGCG_T
Cuts at: 4027 4027
Size: 4594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 468 1426 3265 3449 468
Size: 958 1839 184 1613
Fragments arranged by size:
1839 1613 958 184
MscI TGG'CCA
Cuts at: 712 2353 712
Size: 1641 2953
MslI CAynn'nnrTG
Cuts at: 1304 2581 2708 1304
Size: 1277 127 3190
Fragments arranged by size:
3190 1277 127
MspA1I CmG'CkG
Cuts at: 1020 1143 1703 2017 2377 3392 3637 1020
Size: 123 560 314 360 1015 245 1977
Fragments arranged by size:
1977 1015 560 360 314 245 123
NaeI GCC'GGC
Cuts at: 2773 2773
Size: 4594
NarI GG'CG_CC
Cuts at: 2023 2271 2023
Size: 248 4346
NcoI C'CATG_G
Cuts at: 125 125
Size: 4594
NgoMIV G'CCGG_C
Cuts at: 2771 2771
Size: 4594
NruI TCG'CGA
Cuts at: 2955 2955
Size: 4594
NspI r_CATG'y
Cuts at: 1645 2145 3054 1645
Size: 500 909 3185
Fragments arranged by size:
3185 909 500
PciI A'CATG_T
Cuts at: 3050 3050
Size: 4594
PfoI T'CCnGG_A
Cuts at: 615 886 1076 615
Size: 271 190 4133
Fragments arranged by size:
4133 271 190
PmeI GTTT'AAAC
Cuts at: 1591 1591
Size: 4594
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3733 3765 3733
Size: 32 4562
PpuMI rG'GwC_Cy
Cuts at: 613 1128 613
Size: 515 4079
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1326 1358 1326
Size: 32 4562
PstI C_TGCA'G
Cuts at: 1160 1639 2324 1160
Size: 479 685 3430
Fragments arranged by size:
3430 685 479
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4594
PvuII CAG'CTG
Cuts at: 1020 1143 2017 2377 1020
Size: 123 874 360 3237
Fragments arranged by size:
3237 874 360 123
RsrII CG'GwC_CG
Cuts at: 2787 2787
Size: 4594
SacI G_AGCT'C
Cuts at: 1328 1522 1606 1328
Size: 194 84 4316
Fragments arranged by size:
4316 194 84
SalI G'TCGA_C
Cuts at: 1629 1629
Size: 4594
SapI GCTCTTCn'nnn_
Cuts at: 1175 2615 2825 1175
Size: 1440 210 2944
Fragments arranged by size:
2944 1440 210
SbfI CC_TGCA'GG
Cuts at: 1639 1639
Size: 4594
ScaI AGT'ACT
Cuts at: 4111 4111
Size: 4594
SfcI C'TryA_G
Cuts at: 33 242 1156 1635 2320 3315 3506 33
Size: 209 914 479 685 995 191 1121
Fragments arranged by size:
1121 995 914 685 479 209 191
SfoI GGC'GCC
Cuts at: 2024 2272 2024
Size: 248 4346
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4594
SgrAI Cr'CCGG_yG
Cuts at: 163 163
Size: 4594
SmaI CCC'GGG
Cuts at: 1614 1614
Size: 4594
SmlI C'TyrA_G
Cuts at: 754 1747 2662 3156 3418 754
Size: 993 915 494 262 1930
Fragments arranged by size:
1930 993 915 494 262
SnaBI TAC'GTA
Cuts at: 1584 1584
Size: 4594
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4594
SphI G_CATG'C
Cuts at: 1645 2145 1645
Size: 500 4094
StyI C'CwwG_G
Cuts at: 125 1116 1250 1726 2080 125
Size: 991 134 476 354 2639
Fragments arranged by size:
2639 991 476 354 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 218 2175 2308 2767 2978 218
Size: 1957 133 459 211 1834
Fragments arranged by size:
1957 1834 459 211 133
TatI w'GTAC_w
Cuts at: 715 4109 715
Size: 3394 1200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 214 246 762 794 4111 4143 214
Size: 32 516 32 3317 32 665
Fragments arranged by size:
3317 665 516 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 882 2389 882
Size: 1507 3087
XbaI T'CTAG_A
Cuts at: 1623 1623
Size: 4594
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2087 2087
Size: 4594
XmaI C'CCGG_G
Cuts at: 1612 1612
Size: 4594
XmnI GAAnn'nnTTC
Cuts at: 1072 1289 1072
Size: 217 4377
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PfoI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NdeI NheI NotI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SrfI SspI StuI SwaI XhoI ZraI