Restriction map for
pF1KSDA0634
(Circular) (Six-base) MAPSORT of: pF1KSDA0634.seq Check: 2773 from: 1 to: 7003
pF1KSDA0634 7003 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1980 2725 3104 1980
Size: 745 379 5879
Fragments arranged by size:
5879 745 379
AatII G_ACGT'C
Cuts at: 1174 1174
Size: 7003
AccI GT'mk_AC
Cuts at: 1726 4039 1726
Size: 2313 4690
Acc65I G'GTAC_C
Cuts at: 4017 4017
Size: 7003
AclI AA'CG_TT
Cuts at: 6843 6843
Size: 7003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 3434 4826 5258 6007 6275 340
Size: 3094 1392 432 749 268 1068
Fragments arranged by size:
3094 1392 1068 749 432 268
AflIII A'CryG_T
Cuts at: 3931 3965 4353 5459 6436 3931
Size: 34 388 1106 977 4498
Fragments arranged by size:
4498 1106 977 388 34
AgeI A'CCGG_T
Cuts at: 5391 5391
Size: 7003
AhdI GACnn_n'nnGTC
Cuts at: 1908 3539 1908
Size: 1631 5372
AleI CACnn'nnGTG
Cuts at: 1616 2977 1616
Size: 1361 5642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 538 1267 538
Size: 729 6274
AlwNI CAG_nnn'CTG
Cuts at: 621 1631 2130 2757 2862 3313 4306 5875 621
Size: 1010 499 627 105 451 993 1569 1749
Fragments arranged by size:
1749 1569 1010 993 627 499 451 105
ApaI G_GGCC'C
Cuts at: 2251 3752 2251
Size: 1501 5502
ApaLI G'TGCA_C
Cuts at: 601 3056 5773 601
Size: 2455 2717 1831
Fragments arranged by size:
2717 2455 1831
ApoI r'AATT_y
Cuts at: 2275 3331 4005 6356 6612 2275
Size: 1056 674 2351 256 2666
Fragments arranged by size:
2666 2351 1056 674 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7003
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7003
AvaI C'yCGr_G
Cuts at: 165 201 438 1375 2001 4021 165
Size: 36 237 937 626 2020 3147
Fragments arranged by size:
3147 2020 937 626 237 36
AvrII C'CTAG_G
Cuts at: 1030 1030
Size: 7003
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3597 3630 3597
Size: 33 6970
BamHI G'GATC_C
Cuts at: 4026 4026
Size: 7003
BanI G'GyrC_C
Cuts at: 139 274 379 460 631 3003 4017 4431 4679
Size: 135 105 81 171 2372 1014 414 248
Cuts at: 4679 4714 139
Size: 35 2428
Fragments arranged by size:
2428 2372 1014 414 248 171 135 105
81 35
BanII G_rGCy'C
Cuts at: 173 302 445 707 1180 2251 2468 2714 2810
Size: 129 143 262 473 1071 217 246 96
Cuts at: 2810 2971 3149 3206 3455 3752 4015 5045 173
Size: 161 178 57 249 297 263 1030 2131
Fragments arranged by size:
2131 1071 1030 473 297 263 262 249
246 217 178 161 143 129 96 57
BbeI G_GCGC'C
Cuts at: 143 278 383 4435 4683 143
Size: 135 105 4052 248 2463
Fragments arranged by size:
4052 2463 248 135 105
BbsI GAAGACnn'nnnn_
Cuts at: 328 1850 1904 2343 328
Size: 1522 54 439 4988
Fragments arranged by size:
4988 1522 439 54
BbvCI CC'TCA_GC
Cuts at: 3124 3124
Size: 7003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 814 848 814
Size: 34 6969
BciVI GTATCCnnnnn_n'
Cuts at: 3483 4899 5662 7003 3483
Size: 1416 763 1341 3483
Fragments arranged by size:
3483 1416 1341 763
BclI T'GATC_A
Cuts at: 4521 4521
Size: 7003
BglI GCCn_nnn'nGGC
Cuts at: 1032 2951 1032
Size: 1919 5084
BglII A'GATC_T
Cuts at: 3584 4516 6555 3584
Size: 932 2039 4032
Fragments arranged by size:
4032 2039 932
BlpI GC'TnA_GC
Cuts at: 148 2575 2965 3514 4113 148
Size: 2427 390 549 599 3038
Fragments arranged by size:
3038 2427 599 549 390
Bme1580I G_kGCm'C
Cuts at: 605 974 1376 2017 2251 3060 3373 3752 3854
Size: 369 402 641 234 809 313 379 102
Cuts at: 3854 4626 4719 5777 605
Size: 772 93 1058 1831
Fragments arranged by size:
1831 1058 809 772 641 402 379 369
313 234 102 93
BmgBI CAC'GTC
Cuts at: 648 648
Size: 7003
BmrI ACTGGGnnnn_n'
Cuts at: 1258 1641 4213 4294 4629 6775 1258
Size: 383 2572 81 335 2146 1486
Fragments arranged by size:
2572 2146 1486 383 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5368 5368
Size: 7003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 586 627 643 682 2513 2749 3192 3469
Size: 193 41 16 39 1831 236 443 277
Cuts at: 3469 3747 3789 3802 393
Size: 278 42 13 3594
Fragments arranged by size:
3594 1831 443 278 277 236 193 42
41 39 16 13
Bpu10I CC'TnA_GC
Cuts at: 3081 3124 3081
Size: 43 6960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 2599 2794 3898 4177 5056 5550 5848 1690
Size: 909 195 1104 279 879 494 298 2845
Fragments arranged by size:
2845 1104 909 879 494 298 279 195
BsaI GGTCTCn'nnnn_
Cuts at: 2773 3225 3270 3466 3660 6707 2773
Size: 452 45 196 194 3047 3069
Fragments arranged by size:
3069 3047 452 196 194 45
BsaAI yAC'GTr
Cuts at: 3932 3968 3993 4354 3932
Size: 36 25 361 6581
Fragments arranged by size:
6581 361 36 25
BsaBI GATnn'nnATC
Cuts at: 6 733 4515 6554 6
Size: 727 3782 2039 455
Fragments arranged by size:
3782 2039 727 455
BsaHI Gr'CG_yC
Cuts at: 140 275 380 1171 4432 4680 5384 6880 140
Size: 135 105 791 3261 248 704 1496 263
Fragments arranged by size:
3261 1496 791 704 263 248 135 105
BsaWI w'CCGG_w
Cuts at: 1561 2321 2472 2549 4190 4711 5391 5665 5812
Size: 760 151 77 1641 521 680 274 147
Cuts at: 5812 6223 1561
Size: 411 2341
Fragments arranged by size:
2341 1641 760 680 521 411 274 151
147 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1033 1063 2047 2077 2428 2458 2638 2659 2668
Size: 30 984 30 351 30 180 21 9
Cuts at: 2668 2689 2797 2827 1033
Size: 21 108 30 5209
Fragments arranged by size:
5209 984 351 180 108 30 30 30
30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 736 1027 1036 2035 2203 2452 2659 2920 3889
Size: 291 9 999 168 249 207 261 969
Cuts at: 3889 3952 736
Size: 63 3787
Fragments arranged by size:
3787 999 969 291 261 249 207 168
63 9
BseYI C'CCAG_C
Cuts at: 400 812 903 2327 2396 2734 2790 3744 4426
Size: 412 91 1424 69 338 56 954 682
Cuts at: 4426 5184 5763 6259 400
Size: 758 579 496 1144
Fragments arranged by size:
1424 1144 954 758 682 579 496 412
338 91 69 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1590 1941 2554 3132 544
Size: 1046 351 613 578 4415
Fragments arranged by size:
4415 1046 613 578 351
BsiEI CG_ry'CG
Cuts at: 104 184 4543 4589 5799 104
Size: 80 4359 46 1210 1308
Fragments arranged by size:
4359 1308 1210 80 46
BsiHKAI G_wGCw'C
Cuts at: 605 1382 1835 2161 2714 2810 3060 3082 4015
Size: 777 453 326 553 96 250 22 933
Cuts at: 4015 4793 4983 5777 605
Size: 778 190 794 1831
Fragments arranged by size:
1831 933 794 778 777 553 453 326
250 190 96 22
BsiWI C'GTAC_G
Cuts at: 3968 3968
Size: 7003
BsmBI CGTCTCn'nnnn_
Cuts at: 654 654
Size: 7003
Bsp1286I G_dGCh'C
Cuts at: 173 302 445 605 707 974 1180 1376 1382
Size: 129 143 160 102 267 206 196 6
Cuts at: 1382 1835 2017 2161 2251 2468 2714 2810 2971
Size: 453 182 144 90 217 246 96 161
Cuts at: 2971 3060 3082 3149 3206 3373 3455 3752 3854
Size: 89 22 67 57 167 82 297 102
Cuts at: 3854 4015 4626 4719 4793 4983 5045 5777 173
Size: 161 611 93 74 190 62 732 1399
Fragments arranged by size:
1399 732 611 453 297 267 246 217
206 196 190 182 167 161 161 160
144 143 129 102 102 96 93 90
89 82 74 67 62 57 22 6
BspEI T'CCGG_A
Cuts at: 2472 2549 6223 2472
Size: 77 3674 3252
Fragments arranged by size:
3674 3252 77
BspHI T'CATG_A
Cuts at: 873 1578 6179 873
Size: 705 4601 1697
Fragments arranged by size:
4601 1697 705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1913 1980 2725 3104 4051 4265 4567 4948 1913
Size: 67 745 379 947 214 302 381 3968
Fragments arranged by size:
3968 947 745 381 379 302 214 67
BsrBI CCG'CTC
Cuts at: 50 377 5293 5347 6833 6998 50
Size: 327 4916 54 1486 165 55
Fragments arranged by size:
4916 1486 327 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4913 4913
Size: 7003
BsrFI r'CCGG_y
Cuts at: 136 271 314 457 1985 2430 2945 3174 3986
Size: 135 43 143 1528 445 515 229 812
Cuts at: 3986 4999 5180 5391 136
Size: 1013 181 211 1748
Fragments arranged by size:
1748 1528 1013 812 515 445 229 211
181 143 135 43
BsrGI T'GTAC_A
Cuts at: 3320 3596 3320
Size: 276 6727
BssSI C'ACGA_G
Cuts at: 3494 5272 5632 3494
Size: 1778 360 4865
Fragments arranged by size:
4865 1778 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2078 2130 3177 4378 2078
Size: 52 1047 1201 4703
Fragments arranged by size:
4703 1201 1047 52
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7003
BstEII G'GTnAC_C
Cuts at: 1077 2521 3522 1077
Size: 1444 1001 4558
Fragments arranged by size:
4558 1444 1001
BstXI CCAn_nnnn'nTGG
Cuts at: 2671 2790 3031 3216 2671
Size: 119 241 185 6458
Fragments arranged by size:
6458 241 185 119
BstYI r'GATC_y
Cuts at: 1 878 2618 3584 4026 4493 4516 4851 5097
Size: 877 1740 966 442 467 23 335 246
Cuts at: 5097 6111 6197 6209 6555 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1740 1014 966 877 467 449 442 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2018 3529 2018
Size: 1511 5492
BtgI C'CryG_G
Cuts at: 125 797 1316 1495 2516 2528 3399 125
Size: 672 519 179 1021 12 871 3729
Fragments arranged by size:
3729 1021 871 672 519 179 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 501 750 1885 2670 4951 5132 5295 501
Size: 249 1135 785 2281 181 163 2209
Fragments arranged by size:
2281 2209 1135 785 249 181 163
BtsI GCAGTG_nn'
Cuts at: 214 340 622 1632 2449 2545 3798 4244 214
Size: 126 282 1010 817 96 1253 446 2973
Fragments arranged by size:
2973 1253 1010 817 446 282 126 96
ClaI AT'CG_AT
Cuts at: 3101 3101
Size: 7003
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1869 1904 1869
Size: 35 6968
DraI TTT'AAA
Cuts at: 4000 4000
Size: 7003
DrdI GACnn_nn'nnGTC
Cuts at: 4707 5567 4707
Size: 860 6143
EaeI y'GGCC_r
Cuts at: 128 181 269 1313 1454 1811 2402 2947 4586
Size: 53 88 1044 141 357 591 545 1639
Cuts at: 4586 4760 5151 5178 6525 128
Size: 174 391 27 1347 606
Fragments arranged by size:
1639 1347 1044 606 591 545 391 357
174 141 88 53 27
EagI C'GGCC_G
Cuts at: 181 4586 181
Size: 4405 2598
EarI CTCTTCn'nnn_
Cuts at: 844 1109 5024 5234 844
Size: 265 3915 210 2613
Fragments arranged by size:
3915 2613 265 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 601 1772 5209 5521 5667 6813 601
Size: 1171 3437 312 146 1146 791
Fragments arranged by size:
3437 1171 1146 791 312 146
EcoICRI GAG'CTC
Cuts at: 2712 2808 4013 2712
Size: 96 1205 5702
Fragments arranged by size:
5702 1205 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 393 586 627 643 682 2513 2749 3192
Size: 53 193 41 16 39 1831 236 443
Cuts at: 3192 3434 3469 3747 3789 3802 4826 5258 6007
Size: 242 35 278 42 13 1024 432 749
Cuts at: 6007 6275 340
Size: 268 1068
Fragments arranged by size:
1831 1068 1024 749 443 432 278 268
242 236 193 53 42 41 39 35
16 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2626 2626
Size: 7003
EcoO109I rG'GnC_Cy
Cuts at: 907 1506 1648 2247 2248 2454 2694 3408 3748
Size: 599 142 599 1 206 240 714 340
Cuts at: 3748 4140 907
Size: 392 3770
Fragments arranged by size:
3770 714 599 599 392 340 240 206
142 1
EcoRI G'AATT_C
Cuts at: 3331 4005 3331
Size: 674 6329
EcoRV GAT'ATC
Cuts at: 2499 2499
Size: 7003
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1143 3063 5020 1143
Size: 1920 1957 3126
Fragments arranged by size:
3126 1957 1920
FspI TGC'GCA
Cuts at: 1624 4782 1624
Size: 3158 3845
HaeII r_GCGC'y
Cuts at: 143 278 383 805 2919 3131 4435 4683 5707
Size: 135 105 422 2114 212 1304 248 1024
Cuts at: 5707 6686 143
Size: 979 460
Fragments arranged by size:
2114 1304 1024 979 460 422 248 212
135 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1071 1103 1326 1358 1894 1926 2200 2232 2638
Size: 32 223 32 536 32 274 32 406
Cuts at: 2638 2670 2797 2829 3343 3375 3525 3557 3576
Size: 32 127 32 514 32 150 32 19
Cuts at: 3576 3608 4843 4873 4875 4905 1071
Size: 32 1235 30 2 30 3169
Fragments arranged by size:
3169 1235 536 514 406 274 223 150
127 32 32 32 32 32 32 32
32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 4040 4040
Size: 7003
HindIII A'AGCT_T
Cuts at: 1459 1770 1459
Size: 311 6692
KasI G'GCGC_C
Cuts at: 139 274 379 4431 4679 139
Size: 135 105 4052 248 2463
Fragments arranged by size:
4052 2463 248 135 105
KpnI G_GTAC'C
Cuts at: 4021 4021
Size: 7003
MluI A'CGCG_T
Cuts at: 6436 6436
Size: 7003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1463 2505 3293 5674 5858 1463
Size: 1042 788 2381 184 2608
Fragments arranged by size:
2608 2381 1042 788 184
MscI TGG'CCA
Cuts at: 1315 1813 4762 1315
Size: 498 2949 3556
Fragments arranged by size:
3556 2949 498
MslI CAynn'nnrTG
Cuts at: 520 748 967 1616 2167 2977 3646 4990 5117
Size: 228 219 649 551 810 669 1344 127
Cuts at: 5117 520
Size: 2406
Fragments arranged by size:
2406 1344 810 669 649 551 228 219
127
MspA1I CmG'CkG
Cuts at: 225 264 286 583 672 1203 1574 1817 2112
Size: 39 22 297 89 531 371 243 295
Cuts at: 2112 2400 2427 2757 2964 3388 4112 4426 4786
Size: 288 27 330 207 424 724 314 360
Cuts at: 4786 5801 6046 225
Size: 1015 245 1182
Fragments arranged by size:
1182 1015 724 531 424 371 360 330
314 297 295 288 245 243 207 89
39 27 22
NaeI GCC'GGC
Cuts at: 138 273 316 459 2947 5182 138
Size: 135 43 143 2488 2235 1959
Fragments arranged by size:
2488 2235 1959 143 135 43
NarI GG'CG_CC
Cuts at: 140 275 380 4432 4680 140
Size: 135 105 4052 248 2463
Fragments arranged by size:
4052 2463 248 135 105
NcoI C'CATG_G
Cuts at: 125 1316 1495 2516 2528 3399 125
Size: 1191 179 1021 12 871 3729
Fragments arranged by size:
3729 1191 1021 871 179 12
NgoMIV G'CCGG_C
Cuts at: 136 271 314 457 2945 5180 136
Size: 135 43 143 2488 2235 1959
Fragments arranged by size:
2488 2235 1959 143 135 43
NruI TCG'CGA
Cuts at: 5364 5364
Size: 7003
NspI r_CATG'y
Cuts at: 1372 2335 4054 4554 5463 1372
Size: 963 1719 500 909 2912
Fragments arranged by size:
2912 1719 963 909 500
PciI A'CATG_T
Cuts at: 5459 5459
Size: 7003
PflMI CCAn_nnn'nTGG
Cuts at: 1089 3253 1089
Size: 2164 4839
PfoI T'CCnGG_A
Cuts at: 698 977 992 698
Size: 279 15 6709
Fragments arranged by size:
6709 279 15
PmeI GTTT'AAAC
Cuts at: 4000 4000
Size: 7003
PmlI CAC'GTG
Cuts at: 3932 3932
Size: 7003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 915 947 6142 6174 915
Size: 32 5195 32 1744
Fragments arranged by size:
5195 1744 32 32
PpuMI rG'GwC_Cy
Cuts at: 907 1506 1648 2454 3408 907
Size: 599 142 806 954 4502
Fragments arranged by size:
4502 954 806 599 142
PshAI GACnn'nnGTC
Cuts at: 3262 3262
Size: 7003
PspOMI G'GGCC_C
Cuts at: 2247 3748 2247
Size: 1501 5502
PstI C_TGCA'G
Cuts at: 1854 2123 3160 3173 3284 3891 4048 4733 1854
Size: 269 1037 13 111 607 157 685 4124
Fragments arranged by size:
4124 1037 685 607 269 157 111 13
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7003
PvuII CAG'CTG
Cuts at: 672 1203 1574 1817 2112 2400 2757 2964 3388
Size: 531 371 243 295 288 357 207 424
Cuts at: 3388 4426 4786 672
Size: 1038 360 2889
Fragments arranged by size:
2889 1038 531 424 371 360 357 295
288 243 207
RsrII CG'GwC_CG
Cuts at: 5196 5196
Size: 7003
SacI G_AGCT'C
Cuts at: 2714 2810 4015 2714
Size: 96 1205 5702
Fragments arranged by size:
5702 1205 96
SalI G'TCGA_C
Cuts at: 4038 4038
Size: 7003
SanDI GG'GwC_CC
Cuts at: 907 907
Size: 7003
SapI GCTCTTCn'nnn_
Cuts at: 844 5024 5234 844
Size: 4180 210 2613
Fragments arranged by size:
4180 2613 210
SbfI CC_TGCA'GG
Cuts at: 3891 4048 3891
Size: 157 6846
ScaI AGT'ACT
Cuts at: 3213 6520 3213
Size: 3307 3696
SfcI C'TryA_G
Cuts at: 33 1850 2119 3156 3169 3280 3887 4044 4729
Size: 1817 269 1037 13 111 607 157 685
Cuts at: 4729 5724 5915 33
Size: 995 191 1121
Fragments arranged by size:
1817 1121 1037 995 685 607 269 191
157 111 13
SfoI GGC'GCC
Cuts at: 141 276 381 4433 4681 141
Size: 135 105 4052 248 2463
Fragments arranged by size:
4052 2463 248 135 105
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7003
SgrAI Cr'CCGG_yG
Cuts at: 136 136
Size: 7003
SmaI CCC'GGG
Cuts at: 203 440 4023 203
Size: 237 3583 3183
Fragments arranged by size:
3583 3183 237
SmlI C'TyrA_G
Cuts at: 1669 2614 2809 3913 4156 5071 5565 5827 1669
Size: 945 195 1104 243 915 494 262 2845
Fragments arranged by size:
2845 1104 945 915 494 262 243 195
SnaBI TAC'GTA
Cuts at: 3993 3993
Size: 7003
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7003
SphI G_CATG'C
Cuts at: 4054 4554 4054
Size: 500 6503
StuI AGG'CCT
Cuts at: 1748 3046 1748
Size: 1298 5705
StyI C'CwwG_G
Cuts at: 125 1030 1163 1316 1495 1651 1743 2340 2516
Size: 905 133 153 179 156 92 597 176
Cuts at: 2516 2528 2627 3224 3260 3399 3980 4135 4489
Size: 12 99 597 36 139 581 155 354
Cuts at: 4489 125
Size: 2639
Fragments arranged by size:
2639 905 597 597 581 354 179 176
156 155 153 139 133 99 92 36
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 745 1488 1840 3222 4584 4717 5176 5387 745
Size: 743 352 1382 1362 133 459 211 2361
Fragments arranged by size:
2361 1382 1362 743 459 352 211 133
TatI w'GTAC_w
Cuts at: 638 2192 3211 3320 3596 6518 638
Size: 1554 1019 109 276 2922 1123
Fragments arranged by size:
2922 1554 1123 1019 276 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2428 2460 2659 2691 6520 6552 2428
Size: 32 199 32 3829 32 2879
Fragments arranged by size:
3829 2879 199 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3721 4798 3721
Size: 1077 5926
XbaI T'CTAG_A
Cuts at: 4032 4032
Size: 7003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1806 2404 2488 4496 1806
Size: 598 84 2008 4313
Fragments arranged by size:
4313 2008 598 84
XmaI C'CCGG_G
Cuts at: 201 438 4021 201
Size: 237 3583 3183
Fragments arranged by size:
3583 3183 237
XmnI GAAnn'nnTTC
Cuts at: 1715 2377 1715
Size: 662 6341
ZraI GAC'GTC
Cuts at: 1172 1172
Size: 7003
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI BfrBI BmtI BsmI BssHII
BstZ17I DraIII FseI FspAI HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PsiI PspXI PsrI SacII
SexAI SfiI SrfI SspI SwaI XhoI