Restriction map for
pF1KSDA0617
(Circular) (Six-base) MAPSORT of: pF1KSDA0617.seq Check: 9957 from: 1 to: 5443
pF1KSDA0617 5443 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 542 659 1761 2210 2414 2479 542
Size: 117 1102 449 204 65 3506
Fragments arranged by size:
3506 1102 449 204 117 65
Acc65I G'GTAC_C
Cuts at: 2457 2457
Size: 5443
AclI AA'CG_TT
Cuts at: 1413 5283 1413
Size: 3870 1573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1136 1189 1865 3266 3698 4447 4715 800
Size: 336 53 676 1401 432 749 268 1528
Fragments arranged by size:
1528 1401 749 676 432 336 268 53
AflIII A'CryG_T
Cuts at: 2793 3899 4876 2793
Size: 1106 977 3360
Fragments arranged by size:
3360 1106 977
AgeI A'CCGG_T
Cuts at: 3831 3831
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1686 1686
Size: 5443
AlwNI CAG_nnn'CTG
Cuts at: 2158 2746 4315 2158
Size: 588 1569 3286
Fragments arranged by size:
3286 1569 588
ApaLI G'TGCA_C
Cuts at: 1862 4213 1862
Size: 2351 3092
ApoI r'AATT_y
Cuts at: 408 769 793 966 1582 1970 2445 4796 5052
Size: 361 24 173 616 388 475 2351 256
Cuts at: 5052 408
Size: 799
Fragments arranged by size:
2351 799 616 475 388 361 256 173
24
AseI AT'TA_AT
Cuts at: 20 556 848 1930 20
Size: 536 292 1082 3533
Fragments arranged by size:
3533 1082 536 292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5443
AvaI C'yCGr_G
Cuts at: 2461 2461
Size: 5443
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1595 1628 1595
Size: 33 5410
BamHI G'GATC_C
Cuts at: 176 2466 176
Size: 2290 3153
BanI G'GyrC_C
Cuts at: 2457 2871 3119 3154 2457
Size: 414 248 35 4746
Fragments arranged by size:
4746 414 248 35
BanII G_rGCy'C
Cuts at: 296 2016 2455 3485 296
Size: 1720 439 1030 2254
Fragments arranged by size:
2254 1720 1030 439
BbeI G_GCGC'C
Cuts at: 2875 3123 2875
Size: 248 5195
BbsI GAAGACnn'nnnn_
Cuts at: 952 1013 952
Size: 61 5382
BciVI GTATCCnnnnn_n'
Cuts at: 3339 4102 5443 3339
Size: 763 1341 3339
Fragments arranged by size:
3339 1341 763
BclI T'GATC_A
Cuts at: 456 2112 2961 456
Size: 1656 849 2938
Fragments arranged by size:
2938 1656 849
BfrBI ATG'CAT
Cuts at: 307 307
Size: 5443
BglII A'GATC_T
Cuts at: 1797 2956 4995 1797
Size: 1159 2039 2245
Fragments arranged by size:
2245 2039 1159
BlpI GC'TnA_GC
Cuts at: 2553 2553
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1866 3066 3159 4217 1866
Size: 1200 93 1058 3092
Fragments arranged by size:
3092 1200 1058 93
BmgBI CAC'GTC
Cuts at: 1526 1526
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 2653 2734 3069 5215 2653
Size: 81 335 2146 2881
Fragments arranged by size:
2881 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 358 3808 358
Size: 3450 1993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1254 1759 1254
Size: 505 4938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2617 3496 3990 4288 2617
Size: 879 494 298 3772
Fragments arranged by size:
3772 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 712 1280 5147 712
Size: 568 3867 1008
Fragments arranged by size:
3867 1008 568
BsaAI yAC'GTr
Cuts at: 2433 2794 2433
Size: 361 5082
BsaBI GATnn'nnATC
Cuts at: 6 175 2955 4994 6
Size: 169 2780 2039 455
Fragments arranged by size:
2780 2039 455 169
BsaHI Gr'CG_yC
Cuts at: 2872 3120 3824 5320 2872
Size: 248 704 1496 2995
Fragments arranged by size:
2995 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2630 3151 3831 4105 4252 4663 2630
Size: 521 680 274 147 411 3410
Fragments arranged by size:
3410 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 641 671 1252 1282 641
Size: 30 581 30 4802
Fragments arranged by size:
4802 581 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 304
Size: 5443
BseYI C'CCAG_C
Cuts at: 2866 3624 4203 4699 2866
Size: 758 579 496 3610
Fragments arranged by size:
3610 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 1185 2983 3029 4239 104
Size: 1081 1798 46 1210 1308
Fragments arranged by size:
1798 1308 1210 1081 46
BsiHKAI G_wGCw'C
Cuts at: 296 1649 1866 2455 3233 3423 4217 296
Size: 1353 217 589 778 190 794 1522
Fragments arranged by size:
1522 1353 794 778 589 217 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1222 1222
Size: 5443
Bsp1286I G_dGCh'C
Cuts at: 296 1649 1866 2016 2455 3066 3159 3233 3423
Size: 1353 217 150 439 611 93 74 190
Cuts at: 3423 3485 4217 296
Size: 62 732 1522
Fragments arranged by size:
1522 1353 732 611 439 217 190 150
93 74 62
BspEI T'CCGG_A
Cuts at: 4663 4663
Size: 5443
BspHI T'CATG_A
Cuts at: 4619 4619
Size: 5443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2491 2705 3007 3388 2491
Size: 214 302 381 4546
Fragments arranged by size:
4546 381 302 214
BsrBI CCG'CTC
Cuts at: 50 642 3733 3787 5273 5438 50
Size: 592 3091 54 1486 165 55
Fragments arranged by size:
3091 1486 592 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1376 3353 1376
Size: 1977 3466
BsrFI r'CCGG_y
Cuts at: 3439 3620 3831 3439
Size: 181 211 5051
Fragments arranged by size:
5051 211 181
BsrGI T'GTAC_A
Cuts at: 497 516 1022 497
Size: 19 506 4918
Fragments arranged by size:
4918 506 19
BssSI C'ACGA_G
Cuts at: 3712 4072 3712
Size: 360 5083
BstAPI GCAn_nnn'nTGC
Cuts at: 1899 2818 1899
Size: 919 4524
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5443
BstYI r'GATC_y
Cuts at: 1 176 1164 1797 1840 2466 2933 2956 3291
Size: 175 988 633 43 626 467 23 335
Cuts at: 3291 3537 4551 4637 4649 4995 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 988 633 626 467 449 346 335
246 175 86 43 23 12
BstZ17I GTA'TAC
Cuts at: 2415 2415
Size: 5443
Bsu36I CC'TnA_GG
Cuts at: 2399 2399
Size: 5443
BtgI C'CryG_G
Cuts at: 197 197
Size: 5443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3391 3572 3735 3391
Size: 181 163 5099
Fragments arranged by size:
5099 181 163
BtsI GCAGTG_nn'
Cuts at: 873 2684 873
Size: 1811 3632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1737 1772 1737
Size: 35 5408
DraI TTT'AAA
Cuts at: 1224 2440 1224
Size: 1216 4227
DrdI GACnn_nn'nnGTC
Cuts at: 3147 4007 3147
Size: 860 4583
EaeI y'GGCC_r
Cuts at: 3026 3200 3591 3618 4965 3026
Size: 174 391 27 1347 3504
Fragments arranged by size:
3504 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3026 3026
Size: 5443
EarI CTCTTCn'nnn_
Cuts at: 1165 3464 3674 1165
Size: 2299 210 2934
Fragments arranged by size:
2934 2299 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3649 3961 4107 5253 3649
Size: 312 146 1146 3839
Fragments arranged by size:
3839 1146 312 146
EcoICRI GAG'CTC
Cuts at: 294 2453 294
Size: 2159 3284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1136 1189 1254 1759 1865 3266 3698 4447
Size: 336 53 65 505 106 1401 432 749
Cuts at: 4447 4715 800
Size: 268 1528
Fragments arranged by size:
1528 1401 749 505 432 336 268 106
65 53
EcoNI CCTnn'n_nnAGG
Cuts at: 1086 1086
Size: 5443
EcoO109I rG'GnC_Cy
Cuts at: 228 2580 228
Size: 2352 3091
EcoRI G'AATT_C
Cuts at: 966 1582 2445 966
Size: 616 863 3964
Fragments arranged by size:
3964 863 616
EcoRV GAT'ATC
Cuts at: 1490 1989 1490
Size: 499 4944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 698 1733 3460 698
Size: 1035 1727 2681
Fragments arranged by size:
2681 1727 1035
FspI TGC'GCA
Cuts at: 3222 3222
Size: 5443
HaeII r_GCGC'y
Cuts at: 2875 3123 4147 5126 2875
Size: 248 1024 979 3192
Fragments arranged by size:
3192 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 690 722 3283 3313 3315 3345 690
Size: 32 2561 30 2 30 2788
Fragments arranged by size:
2788 2561 32 30 30 2
HincII GTy'rAC
Cuts at: 1143 1762 2480 1143
Size: 619 718 4106
Fragments arranged by size:
4106 718 619
HindIII A'AGCT_T
Cuts at: 446 446
Size: 5443
KasI G'GCGC_C
Cuts at: 2871 3119 2871
Size: 248 5195
KpnI G_GTAC'C
Cuts at: 2461 2461
Size: 5443
MfeI C'AATT_G
Cuts at: 902 902
Size: 5443
MluI A'CGCG_T
Cuts at: 4876 4876
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1836 2201 4114 4298 1836
Size: 365 1913 184 2981
Fragments arranged by size:
2981 1913 365 184
MscI TGG'CCA
Cuts at: 3202 3202
Size: 5443
MslI CAynn'nnrTG
Cuts at: 169 329 493 1573 1686 1930 3430 3557 169
Size: 160 164 1080 113 244 1500 127 2055
Fragments arranged by size:
2055 1500 1080 244 164 160 127 113
MspA1I CmG'CkG
Cuts at: 2049 2552 2866 3226 4241 4486 2049
Size: 503 314 360 1015 245 3006
Fragments arranged by size:
3006 1015 503 360 314 245
NaeI GCC'GGC
Cuts at: 3622 3622
Size: 5443
NarI GG'CG_CC
Cuts at: 2872 3120 2872
Size: 248 5195
NcoI C'CATG_G
Cuts at: 197 197
Size: 5443
NdeI CA'TA_TG
Cuts at: 983 1784 2421 983
Size: 801 637 4005
Fragments arranged by size:
4005 801 637
NgoMIV G'CCGG_C
Cuts at: 3620 3620
Size: 5443
NruI TCG'CGA
Cuts at: 3804 3804
Size: 5443
NsiI A_TGCA'T
Cuts at: 309 309
Size: 5443
NspI r_CATG'y
Cuts at: 1023 1717 2494 2994 3903 1023
Size: 694 777 500 909 2563
Fragments arranged by size:
2563 909 777 694 500
PciI A'CATG_T
Cuts at: 3899 3899
Size: 5443
PfoI T'CCnGG_A
Cuts at: 1193 1193
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2440 2440
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4582 4614 4582
Size: 32 5411
PpuMI rG'GwC_Cy
Cuts at: 228 228
Size: 5443
PsiI TTA'TAA
Cuts at: 1421 1421
Size: 5443
PstI C_TGCA'G
Cuts at: 768 1797 2488 3173 768
Size: 1029 691 685 3038
Fragments arranged by size:
3038 1029 691 685
PvuI CG_AT'CG
Cuts at: 104 1185 104
Size: 1081 4362
PvuII CAG'CTG
Cuts at: 2866 3226 2866
Size: 360 5083
RsrII CG'GwC_CG
Cuts at: 3636 3636
Size: 5443
SacI G_AGCT'C
Cuts at: 296 2455 296
Size: 2159 3284
SalI G'TCGA_C
Cuts at: 1760 2478 1760
Size: 718 4725
SapI GCTCTTCn'nnn_
Cuts at: 3464 3674 3464
Size: 210 5233
SbfI CC_TGCA'GG
Cuts at: 2488 2488
Size: 5443
ScaI AGT'ACT
Cuts at: 4960 4960
Size: 5443
SfcI C'TryA_G
Cuts at: 33 297 764 950 1597 1793 2017 2243 2484
Size: 264 467 186 647 196 224 226 241
Cuts at: 2484 3169 4164 4355 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 685 647 467 264 241 226
224 196 191 186
SfoI GGC'GCC
Cuts at: 2873 3121 2873
Size: 248 5195
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5443
SmaI CCC'GGG
Cuts at: 2463 2463
Size: 5443
SmlI C'TyrA_G
Cuts at: 2596 3511 4005 4267 2596
Size: 915 494 262 3772
Fragments arranged by size:
3772 915 494 262
SnaBI TAC'GTA
Cuts at: 2433 2433
Size: 5443
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5443
SphI G_CATG'C
Cuts at: 2494 2994 2494
Size: 500 4943
SspI AAT'ATT
Cuts at: 1358 2383 1358
Size: 1025 4418
StyI C'CwwG_G
Cuts at: 197 1011 2575 2929 197
Size: 814 1564 354 2711
Fragments arranged by size:
2711 1564 814 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 578 3024 3157 3616 3827 578
Size: 2446 133 459 211 2194
Fragments arranged by size:
2446 2194 459 211 133
TatI w'GTAC_w
Cuts at: 402 497 516 978 1022 4958 402
Size: 95 19 462 44 3936 887
Fragments arranged by size:
3936 887 462 95 44 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4960 4992 4960
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 3238 3238
Size: 5443
XbaI T'CTAG_A
Cuts at: 2263 2472 2263
Size: 209 5234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2936 2936
Size: 5443
XmaI C'CCGG_G
Cuts at: 2461 2461
Size: 5443
XmnI GAAnn'nnTTC
Cuts at: 773 773
Size: 5443
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NsiI NspI PciI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BglI BmtI Bpu10I BsgI
BsiWI BsmI BssHII BstEII BstXI ClaI DraIII FseI
FspAI HpaI NheI NotI PacI PasI PflMI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI