Restriction map for
pF1KSDA0592
(Circular) (Six-base) MAPSORT of: pF1KSDA0592.seq Check: 5158 from: 1 to: 7099
pF1KSDA0592 7099 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 3177 4135 3177
Size: 958 6141
Acc65I G'GTAC_C
Cuts at: 4113 4113
Size: 7099
AclI AA'CG_TT
Cuts at: 6939 6939
Size: 7099
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1106 1426 3014 4922 5354 6103 6371 1106
Size: 320 1588 1908 432 749 268 1834
Fragments arranged by size:
1908 1834 1588 749 432 320 268
AflIII A'CryG_T
Cuts at: 4449 5555 6532 4449
Size: 1106 977 5016
Fragments arranged by size:
5016 1106 977
AgeI A'CCGG_T
Cuts at: 5487 5487
Size: 7099
AhdI GACnn_n'nnGTC
Cuts at: 2043 2043
Size: 7099
AleI CACnn'nnGTG
Cuts at: 1375 1375
Size: 7099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3206 3206
Size: 7099
AlwNI CAG_nnn'CTG
Cuts at: 156 1348 4402 5971 156
Size: 1192 3054 1569 1284
Fragments arranged by size:
3054 1569 1284 1192
ApaLI G'TGCA_C
Cuts at: 5869 5869
Size: 7099
ApoI r'AATT_y
Cuts at: 271 2386 2940 4101 6452 6708 271
Size: 2115 554 1161 2351 256 662
Fragments arranged by size:
2351 2115 1161 662 554 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7099
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7099
AvaI C'yCGr_G
Cuts at: 3079 4117 3079
Size: 1038 6061
BamHI G'GATC_C
Cuts at: 4122 4122
Size: 7099
BanI G'GyrC_C
Cuts at: 159 1518 2289 2716 3277 4113 4527 4775 4810
Size: 1359 771 427 561 836 414 248 35
Cuts at: 4810 159
Size: 2448
Fragments arranged by size:
2448 1359 836 771 561 427 414 248
35
BanII G_rGCy'C
Cuts at: 176 1949 3571 4111 5141 176
Size: 1773 1622 540 1030 2134
Fragments arranged by size:
2134 1773 1622 1030 540
BbeI G_GCGC'C
Cuts at: 3281 4531 4779 3281
Size: 1250 248 5601
Fragments arranged by size:
5601 1250 248
BbsI GAAGACnn'nnnn_
Cuts at: 363 490 1093 1301 2132 2179 2207 2634 3335
Size: 127 603 208 831 47 28 427 701
Cuts at: 3335 363
Size: 4127
Fragments arranged by size:
4127 831 701 603 427 208 127 47
28
BbvCI CC'TCA_GC
Cuts at: 458 3230 458
Size: 2772 4327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2599 2633 2599
Size: 34 7065
BciVI GTATCCnnnnn_n'
Cuts at: 3979 4995 5758 7099 3979
Size: 1016 763 1341 3979
Fragments arranged by size:
3979 1341 1016 763
BclI T'GATC_A
Cuts at: 4617 4617
Size: 7099
BglII A'GATC_T
Cuts at: 507 1590 2437 2987 4612 6651 507
Size: 1083 847 550 1625 2039 955
Fragments arranged by size:
2039 1625 1083 955 847 550
BlpI GC'TnA_GC
Cuts at: 4209 4209
Size: 7099
Bme1580I G_kGCm'C
Cuts at: 3481 4722 4815 5873 3481
Size: 1241 93 1058 4707
Fragments arranged by size:
4707 1241 1058 93
BmgBI CAC'GTC
Cuts at: 1829 1829
Size: 7099
BmrI ACTGGGnnnn_n'
Cuts at: 396 4309 4390 4725 6871 396
Size: 3913 81 335 2146 624
Fragments arranged by size:
3913 2146 624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1194 1770 2206 2298 5464 1194
Size: 576 436 92 3166 2829
Fragments arranged by size:
3166 2829 576 436 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1214 1307 2062 3410 1214
Size: 93 755 1348 4903
Fragments arranged by size:
4903 1348 755 93
Bpu10I CC'TnA_GC
Cuts at: 458 1424 2444 3119 3212 3230 458
Size: 966 1020 675 93 18 4327
Fragments arranged by size:
4327 1020 966 675 93 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1684 4273 5152 5646 5944 436
Size: 1248 2589 879 494 298 1591
Fragments arranged by size:
2589 1591 1248 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 6803 6803
Size: 7099
BsaAI yAC'GTr
Cuts at: 4089 4450 4089
Size: 361 6738
BsaBI GATnn'nnATC
Cuts at: 6 2780 3608 4611 6650 6
Size: 2774 828 1003 2039 455
Fragments arranged by size:
2774 2039 1003 828 455
BsaHI Gr'CG_yC
Cuts at: 166 3278 4528 4776 5480 6976 166
Size: 3112 1250 248 704 1496 289
Fragments arranged by size:
3112 1496 1250 704 289 248
BsaWI w'CCGG_w
Cuts at: 134 4286 4807 5487 5761 5908 6319 134
Size: 4152 521 680 274 147 411 914
Fragments arranged by size:
4152 914 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 175 193 205 586 616 1280 1310 1984
Size: 12 18 12 381 30 664 30 674
Cuts at: 1984 2014 3039 3069 3943 3973 163
Size: 30 1025 30 874 30 3289
Fragments arranged by size:
3289 1025 874 674 664 381 30 30
30 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 454 820 823 943 1060 1258 1318 1762
Size: 237 366 3 120 117 198 60 444
Cuts at: 1762 2005 2182 2228 3355 3951 217
Size: 243 177 46 1127 596 3365
Fragments arranged by size:
3365 1127 596 444 366 243 237 198
177 120 117 60 46 3
BseYI C'CCAG_C
Cuts at: 1338 2804 2955 4522 5280 5859 6355 1338
Size: 1466 151 1567 758 579 496 2082
Fragments arranged by size:
2082 1567 1466 758 579 496 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3212 3347 3989 3212
Size: 135 642 6322
Fragments arranged by size:
6322 642 135
BsiEI CG_ry'CG
Cuts at: 104 190 242 4639 4685 5895 104
Size: 86 52 4397 46 1210 1308
Fragments arranged by size:
4397 1308 1210 86 52 46
BsiHKAI G_wGCw'C
Cuts at: 1949 2635 4111 4889 5079 5873 1949
Size: 686 1476 778 190 794 3175
Fragments arranged by size:
3175 1476 794 778 686 190
BsmI GAATG_Cn'
Cuts at: 2460 4074 2460
Size: 1614 5485
Bsp1286I G_dGCh'C
Cuts at: 176 1949 2635 3481 3571 4111 4722 4815 4889
Size: 1773 686 846 90 540 611 93 74
Cuts at: 4889 5079 5141 5873 176
Size: 190 62 732 1402
Fragments arranged by size:
1773 1402 846 732 686 611 540 190
93 90 74 62
BspEI T'CCGG_A
Cuts at: 6319 6319
Size: 7099
BspHI T'CATG_A
Cuts at: 6275 6275
Size: 7099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2033 4147 4361 4663 5044 2033
Size: 2114 214 302 381 4088
Fragments arranged by size:
4088 2114 381 302 214
BsrBI CCG'CTC
Cuts at: 50 186 5389 5443 6929 7094 50
Size: 136 5203 54 1486 165 55
Fragments arranged by size:
5203 1486 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 2150 3257 5009 2150
Size: 1107 1752 4240
Fragments arranged by size:
4240 1752 1107
BsrFI r'CCGG_y
Cuts at: 162 3094 5095 5276 5487 162
Size: 2932 2001 181 211 1774
Fragments arranged by size:
2932 2001 1774 211 181
BssSI C'ACGA_G
Cuts at: 488 5368 5728 488
Size: 4880 360 1859
Fragments arranged by size:
4880 1859 360
BstAPI GCAn_nnn'nTGC
Cuts at: 4474 4474
Size: 7099
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7099
BstEII G'GTnAC_C
Cuts at: 1674 1674
Size: 7099
BstYI r'GATC_y
Cuts at: 1 206 507 654 1590 2437 2987 3484 4122
Size: 205 301 147 936 847 550 497 638
Cuts at: 4122 4589 4612 4947 5193 6207 6293 6305 6651
Size: 467 23 335 246 1014 86 12 346
Cuts at: 6651 1
Size: 449
Fragments arranged by size:
1014 936 847 638 550 497 467 449
346 335 301 246 205 147 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 1065 2933 1065
Size: 1868 5231
BtgI C'CryG_G
Cuts at: 190 1028 2488 3051 3473 3494 190
Size: 838 1460 563 422 21 3795
Fragments arranged by size:
3795 1460 838 563 422 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1601 2324 5047 5228 5391 1601
Size: 723 2723 181 163 3309
Fragments arranged by size:
3309 2723 723 181 163
BtsI GCAGTG_nn'
Cuts at: 768 1559 1989 3606 4340 768
Size: 791 430 1617 734 3527
Fragments arranged by size:
3527 1617 791 734 430
DraI TTT'AAA
Cuts at: 4096 4096
Size: 7099
DraIII CAC_nnn'GTG
Cuts at: 4041 4041
Size: 7099
DrdI GACnn_nn'nnGTC
Cuts at: 4803 5663 4803
Size: 860 6239
EaeI y'GGCC_r
Cuts at: 187 239 3294 3301 4682 4856 5247 5274 6621
Size: 52 3055 7 1381 174 391 27 1347
Cuts at: 6621 187
Size: 665
Fragments arranged by size:
3055 1381 1347 665 391 174 52 27
7
EagI C'GGCC_G
Cuts at: 187 239 4682 187
Size: 52 4443 2604
Fragments arranged by size:
4443 2604 52
EarI CTCTTCn'nnn_
Cuts at: 788 797 878 1127 1261 1853 1919 2312 2540
Size: 9 81 249 134 592 66 393 228
Cuts at: 2540 2648 5120 5330 788
Size: 108 2472 210 2557
Fragments arranged by size:
2557 2472 592 393 249 228 210 134
108 81 66 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5305 5617 5763 6909 5305
Size: 312 146 1146 5495
Fragments arranged by size:
5495 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1947 4109 1947
Size: 2162 4937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1106 1214 1307 1426 2062 3014 3410 4922 5354
Size: 108 93 119 636 952 396 1512 432
Cuts at: 5354 6103 6371 1106
Size: 749 268 1834
Fragments arranged by size:
1834 1512 952 749 636 432 396 268
119 108 93
EcoNI CCTnn'n_nnAGG
Cuts at: 3371 3584 3371
Size: 213 6886
EcoO109I rG'GnC_Cy
Cuts at: 296 2072 3253 3345 4236 296
Size: 1776 1181 92 891 3159
Fragments arranged by size:
3159 1776 1181 891 92
EcoRI G'AATT_C
Cuts at: 271 4101 271
Size: 3830 3269
EcoRV GAT'ATC
Cuts at: 582 1569 3867 582
Size: 987 2298 3814
Fragments arranged by size:
3814 2298 987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1695 2158 2677 5116 1695
Size: 463 519 2439 3678
Fragments arranged by size:
3678 2439 519 463
FspI TGC'GCA
Cuts at: 4878 4878
Size: 7099
HaeII r_GCGC'y
Cuts at: 3281 4531 4779 5803 6782 3281
Size: 1250 248 1024 979 3598
Fragments arranged by size:
3598 1250 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 796 828 1297 1329 1498 1530 1890 1922 2029
Size: 32 469 32 169 32 360 32 107
Cuts at: 2029 2061 3039 3071 3093 3125 3706 3738 3943
Size: 32 978 32 22 32 581 32 205
Cuts at: 3943 3975 4939 4969 4971 5001 796
Size: 32 964 30 2 30 2894
Fragments arranged by size:
2894 978 964 581 469 360 205 169
107 32 32 32 32 32 32 32
32 32 30 30 22 2
HincII GTy'rAC
Cuts at: 2703 2856 4136 2703
Size: 153 1280 5666
Fragments arranged by size:
5666 1280 153
HpaI GTT'AAC
Cuts at: 2856 2856
Size: 7099
KasI G'GCGC_C
Cuts at: 3277 4527 4775 3277
Size: 1250 248 5601
Fragments arranged by size:
5601 1250 248
KpnI G_GTAC'C
Cuts at: 4117 4117
Size: 7099
MluI A'CGCG_T
Cuts at: 6532 6532
Size: 7099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 149 4070 5770 5954 149
Size: 3921 1700 184 1294
Fragments arranged by size:
3921 1700 1294 184
MscI TGG'CCA
Cuts at: 3296 4858 3296
Size: 1562 5537
MslI CAynn'nnrTG
Cuts at: 836 868 1375 2487 5086 5213 836
Size: 32 507 1112 2599 127 2722
Fragments arranged by size:
2722 2599 1112 507 127 32
MspA1I CmG'CkG
Cuts at: 1885 2959 2977 3355 3361 4208 4522 4882 5897
Size: 1074 18 378 6 847 314 360 1015
Cuts at: 5897 6142 1885
Size: 245 2842
Fragments arranged by size:
2842 1074 1015 847 378 360 314 245
18 6
NaeI GCC'GGC
Cuts at: 164 5278 164
Size: 5114 1985
NarI GG'CG_CC
Cuts at: 3278 4528 4776 3278
Size: 1250 248 5601
Fragments arranged by size:
5601 1250 248
NcoI C'CATG_G
Cuts at: 1028 2488 1028
Size: 1460 5639
NdeI CA'TA_TG
Cuts at: 2914 2914
Size: 7099
NgoMIV G'CCGG_C
Cuts at: 162 5276 162
Size: 5114 1985
NruI TCG'CGA
Cuts at: 5460 5460
Size: 7099
NspI r_CATG'y
Cuts at: 4150 4650 5559 4150
Size: 500 909 5690
Fragments arranged by size:
5690 909 500
PasI CC'CwG_GG
Cuts at: 2060 2060
Size: 7099
PciI A'CATG_T
Cuts at: 5555 5555
Size: 7099
PflMI CCAn_nnn'nTGG
Cuts at: 156 2749 2811 3057 3553 156
Size: 2593 62 246 496 3702
Fragments arranged by size:
3702 2593 496 246 62
PfoI T'CCnGG_A
Cuts at: 332 524 2645 3665 332
Size: 192 2121 1020 3766
Fragments arranged by size:
3766 2121 1020 192
PmeI GTTT'AAAC
Cuts at: 4096 4096
Size: 7099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6238 6270 6238
Size: 32 7067
PpuMI rG'GwC_Cy
Cuts at: 296 2072 3253 296
Size: 1776 1181 4142
Fragments arranged by size:
4142 1776 1181
PstI C_TGCA'G
Cuts at: 4144 4829 4144
Size: 685 6414
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7099
PvuII CAG'CTG
Cuts at: 1885 3355 4522 4882 1885
Size: 1470 1167 360 4102
Fragments arranged by size:
4102 1470 1167 360
RsrII CG'GwC_CG
Cuts at: 5292 5292
Size: 7099
SacI G_AGCT'C
Cuts at: 1949 4111 1949
Size: 2162 4937
SalI G'TCGA_C
Cuts at: 4134 4134
Size: 7099
SanDI GG'GwC_CC
Cuts at: 2072 2072
Size: 7099
SapI GCTCTTCn'nnn_
Cuts at: 2648 5120 5330 2648
Size: 2472 210 4417
Fragments arranged by size:
4417 2472 210
SbfI CC_TGCA'GG
Cuts at: 4144 4144
Size: 7099
ScaI AGT'ACT
Cuts at: 449 6616 449
Size: 6167 932
SfcI C'TryA_G
Cuts at: 33 256 265 755 4140 4825 5820 6011 33
Size: 223 9 490 3385 685 995 191 1121
Fragments arranged by size:
3385 1121 995 685 490 223 191 9
SfoI GGC'GCC
Cuts at: 3279 4529 4777 3279
Size: 1250 248 5601
Fragments arranged by size:
5601 1250 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7099
SmaI CCC'GGG
Cuts at: 3081 4119 3081
Size: 1038 6061
SmlI C'TyrA_G
Cuts at: 451 1699 4252 5167 5661 5923 451
Size: 1248 2553 915 494 262 1627
Fragments arranged by size:
2553 1627 1248 915 494 262
SnaBI TAC'GTA
Cuts at: 4089 4089
Size: 7099
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7099
SphI G_CATG'C
Cuts at: 4150 4650 4150
Size: 500 6599
SspI AAT'ATT
Cuts at: 2007 2007
Size: 7099
StyI C'CwwG_G
Cuts at: 1028 1438 2093 2156 2372 2488 3367 3512 4231
Size: 410 655 63 216 116 879 145 719
Cuts at: 4231 4585 1028
Size: 354 3542
Fragments arranged by size:
3542 879 719 655 410 354 216 145
116 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1180 3541 4680 4813 5272 5483 1180
Size: 2361 1139 133 459 211 2796
Fragments arranged by size:
2796 2361 1139 459 211 133
TatI w'GTAC_w
Cuts at: 447 6614 447
Size: 6167 932
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 175 195 207 1535 1567 1984 2016 2235
Size: 12 20 12 1328 32 417 32 219
Cuts at: 2235 2267 4027 4059 6616 6648 163
Size: 32 1760 32 2557 32 614
Fragments arranged by size:
2557 1760 1328 614 417 219 32 32
32 32 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 1902 4894 1902
Size: 2992 4107
XbaI T'CTAG_A
Cuts at: 4128 4128
Size: 7099
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1035 3546 3912 4592 1035
Size: 2511 366 680 3542
Fragments arranged by size:
3542 2511 680 366
XmaI C'CCGG_G
Cuts at: 3079 4117 3079
Size: 1038 6061
XmnI GAAnn'nnTTC
Cuts at: 1119 2888 1119
Size: 1769 5330
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BfrBI BglI BmtI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI FseI FspAI HindIII MfeI
NheI NotI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI