Restriction map for
pF1KSDA0585
(Circular) (Six-base) MAPSORT of: pF1KSDA0585.seq Check: 1980 from: 1 to: 4379
pF1KSDA0585 4379 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 2204 2636 3383 3651 1105
Size: 1099 432 747 268 1833
Fragments arranged by size:
1833 1099 747 432 268
AfeI AGC'GCT
Cuts at: 739 739
Size: 4379
AflIII A'CryG_T
Cuts at: 1731 2835 3812 1731
Size: 1104 977 2298
Fragments arranged by size:
2298 1104 977
AgeI A'CCGG_T
Cuts at: 2767 2767
Size: 4379
AlwNI CAG_nnn'CTG
Cuts at: 710 1684 3251 710
Size: 974 1567 1838
Fragments arranged by size:
1838 1567 974
ApaLI G'TGCA_C
Cuts at: 1237 3149 1237
Size: 1912 2467
ApoI r'AATT_y
Cuts at: 298 877 984 1383 3732 3988 298
Size: 579 107 399 2349 256 689
Fragments arranged by size:
2349 689 579 399 256 107
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 497 788 1087 1399 497
Size: 291 299 312 3477
Fragments arranged by size:
3477 312 299 291
BamHI G'GATC_C
Cuts at: 1216 1404 1216
Size: 188 4191
BanI G'GyrC_C
Cuts at: 579 743 1395 1809 2057 2092 579
Size: 164 652 414 248 35 2866
Fragments arranged by size:
2866 652 414 248 164 35
BanII G_rGCy'C
Cuts at: 185 241 1362 1393 2423 185
Size: 56 1121 31 1030 2141
Fragments arranged by size:
2141 1121 1030 56 31
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 575 575
Size: 4379
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3038 4379 2277
Size: 761 1341 2277
Fragments arranged by size:
2277 1341 761
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglII A'GATC_T
Cuts at: 1894 3931 1894
Size: 2037 2342
BlpI GC'TnA_GC
Cuts at: 235 1491 235
Size: 1256 3123
Bme1580I G_kGCm'C
Cuts at: 1241 2004 2097 3153 1241
Size: 763 93 1056 2467
Fragments arranged by size:
2467 1056 763 93
BmgBI CAC'GTC
Cuts at: 1262 1262
Size: 4379
BmrI ACTGGGnnnn_n'
Cuts at: 686 1591 1672 2007 4151 686
Size: 905 81 335 2144 914
Fragments arranged by size:
2144 914 905 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 246 1341 2746 246
Size: 1095 1405 1879
Fragments arranged by size:
1879 1405 1095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1137 1158 1164 1314 406
Size: 731 21 6 150 3471
Fragments arranged by size:
3471 731 150 21 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1555 2434 2926 3224 169
Size: 1386 879 492 298 1324
Fragments arranged by size:
1386 1324 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1032 4083 1032
Size: 3051 1328
BsaAI yAC'GTr
Cuts at: 1371 1732 1371
Size: 361 4018
BsaBI GATnn'nnATC
Cuts at: 6 685 1893 3930 6
Size: 679 1208 2037 455
Fragments arranged by size:
2037 1208 679 455
BsaHI Gr'CG_yC
Cuts at: 1810 2058 2760 4256 1810
Size: 248 702 1496 1933
Fragments arranged by size:
1933 1496 702 248
BsaWI w'CCGG_w
Cuts at: 670 1568 2089 2767 3041 3188 3599 670
Size: 898 521 678 274 147 411 1450
Fragments arranged by size:
1450 898 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 747 777 902 932 1276 1306 747
Size: 30 125 30 344 30 3820
Fragments arranged by size:
3820 344 125 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1161 1269 1317 1161
Size: 108 48 4223
Fragments arranged by size:
4223 108 48
BseYI C'CCAG_C
Cuts at: 616 1804 2562 3139 3635 616
Size: 1188 758 577 496 1360
Fragments arranged by size:
1360 1188 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1284 1284
Size: 4379
BsiEI CG_ry'CG
Cuts at: 104 178 248 1921 1967 3175 104
Size: 74 70 1673 46 1208 1308
Fragments arranged by size:
1673 1308 1208 74 70 46
BsiHKAI G_wGCw'C
Cuts at: 185 496 1085 1241 1393 2171 2361 3153 185
Size: 311 589 156 152 778 190 792 1411
Fragments arranged by size:
1411 792 778 589 311 190 156 152
BsmI GAATG_Cn'
Cuts at: 351 720 351
Size: 369 4010
BsmBI CGTCTCn'nnnn_
Cuts at: 785 785
Size: 4379
Bsp1286I G_dGCh'C
Cuts at: 185 241 496 1085 1241 1362 1393 2004 2097
Size: 56 255 589 156 121 31 611 93
Cuts at: 2097 2171 2361 2423 3153 185
Size: 74 190 62 730 1411
Fragments arranged by size:
1411 730 611 589 255 190 156 121
93 74 62 56 31
BspEI T'CCGG_A
Cuts at: 670 3599 670
Size: 2929 1450
BspHI T'CATG_A
Cuts at: 3555 3555
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1429 1643 1945 2326 1429
Size: 214 302 381 3482
Fragments arranged by size:
3482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 309 2671 2725 4209 4374 50
Size: 259 2362 54 1484 165 55
Fragments arranged by size:
2362 1484 259 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2291 2291
Size: 4379
BsrFI r'CCGG_y
Cuts at: 2377 2558 2767 2377
Size: 181 209 3989
Fragments arranged by size:
3989 209 181
BssSI C'ACGA_G
Cuts at: 2650 3008 2650
Size: 358 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 1756
Size: 4379
BstBI TT'CG_AA
Cuts at: 110 2740 110
Size: 2630 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 616 935 616
Size: 319 4060
BstYI r'GATC_y
Cuts at: 1 1216 1404 1871 1894 2229 2475 3487 3573
Size: 1215 188 467 23 335 246 1012 86
Cuts at: 3573 3585 3931 1
Size: 12 346 449
Fragments arranged by size:
1215 1012 467 449 346 335 246 188
86 23 12
BtgI C'CryG_G
Cuts at: 248 332 248
Size: 84 4295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1241 2329 2510 2673 1241
Size: 1088 181 163 2947
Fragments arranged by size:
2947 1088 181 163
BtsI GCAGTG_nn'
Cuts at: 1622 1622
Size: 4379
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 1306 2085 2943 1306
Size: 779 858 2742
Fragments arranged by size:
2742 858 779
EaeI y'GGCC_r
Cuts at: 175 245 868 1964 2138 2529 2556 3901 175
Size: 70 623 1096 174 391 27 1345 653
Fragments arranged by size:
1345 1096 653 623 391 174 70 27
EagI C'GGCC_G
Cuts at: 175 245 1964 175
Size: 70 1719 2590
Fragments arranged by size:
2590 1719 70
EarI CTCTTCn'nnn_
Cuts at: 1167 1246 2402 2612 1167
Size: 79 1156 210 2934
Fragments arranged by size:
2934 1156 210 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 268 2587 2897 3043 4189 268
Size: 2319 310 146 1146 458
Fragments arranged by size:
2319 1146 458 310 146
EcoICRI GAG'CTC
Cuts at: 183 1391 183
Size: 1208 3171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1105 1137 1158 1164 1314 2204 2636 3383
Size: 699 32 21 6 150 890 432 747
Cuts at: 3383 3651 406
Size: 268 1134
Fragments arranged by size:
1134 890 747 699 432 268 150 32
21 6
EcoO109I rG'GnC_Cy
Cuts at: 632 1518 632
Size: 886 3493
EcoRI G'AATT_C
Cuts at: 877 1383 877
Size: 506 3873
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 350 368 2160 350
Size: 18 1792 2569
Fragments arranged by size:
2569 1792 18
HaeII r_GCGC'y
Cuts at: 393 741 1813 2061 3083 4062 393
Size: 348 1072 248 1022 979 710
Fragments arranged by size:
1072 1022 979 710 348 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 433 465 902 934 1183 1195 1201
Size: 32 55 32 437 32 249 12 6
Cuts at: 1201 1215 1227 1233 1276 1308 2221 2251 2253
Size: 14 12 6 43 32 913 30 2
Cuts at: 2253 2283 346
Size: 30 2442
Fragments arranged by size:
2442 913 437 249 55 43 32 32
32 32 30 30 14 12 12 6
6 2
HincII GTy'rAC
Cuts at: 1116 1418 1116
Size: 302 4077
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 886 1169 1220 3050 3234 886
Size: 283 51 1830 184 2031
Fragments arranged by size:
2031 1830 283 184 51
MscI TGG'CCA
Cuts at: 870 2140 870
Size: 1270 3109
MslI CAynn'nnrTG
Cuts at: 781 2368 2495 781
Size: 1587 127 2665
Fragments arranged by size:
2665 1587 127
MspA1I CmG'CkG
Cuts at: 285 1490 1804 2164 3177 3422 285
Size: 1205 314 360 1013 245 1242
Fragments arranged by size:
1242 1205 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NspI r_CATG'y
Cuts at: 946 1432 1932 2839 946
Size: 486 500 907 2486
Fragments arranged by size:
2486 907 500 486
PasI CC'CwG_GG
Cuts at: 767 767
Size: 4379
PciI A'CATG_T
Cuts at: 2835 2835
Size: 4379
PflMI CCAn_nnn'nTGG
Cuts at: 710 710
Size: 4379
PfoI T'CCnGG_A
Cuts at: 1330 1330
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 4347
PstI C_TGCA'G
Cuts at: 1426 2111 1426
Size: 685 3694
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 285 1804 2164 285
Size: 1519 360 2500
Fragments arranged by size:
2500 1519 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 185 1393 185
Size: 1208 3171
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SexAI A'CCwGG_T
Cuts at: 826 826
Size: 4379
SfcI C'TryA_G
Cuts at: 33 1422 2107 3100 3291 33
Size: 1389 685 993 191 1121
Fragments arranged by size:
1389 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 184 497 1534 2449 2941 3203 184
Size: 313 1037 915 492 262 1360
Fragments arranged by size:
1360 1037 915 492 313 262
SnaBI TAC'GTA
Cuts at: 1371 1371
Size: 4379
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
StyI C'CwwG_G
Cuts at: 1513 1867 1513
Size: 354 4025
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 994 1962 2095 2554 2763 994
Size: 968 133 459 209 2610
Fragments arranged by size:
2610 968 459 209 133
TatI w'GTAC_w
Cuts at: 482 3894 482
Size: 3412 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4347
Tth111I GACn'n_nGTC
Cuts at: 1306 2176 1306
Size: 870 3509
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 935 1008 1874 935
Size: 73 866 3440
Fragments arranged by size:
3440 866 73
XhoI C'TCGA_G
Cuts at: 497 497
Size: 4379
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI SspI StuI SwaI XmnI
ZraI