Restriction map for
pF1KSDA0547
(Circular) (Six-base) MAPSORT of: pF1KSDA0547.seq Check: 1084 from: 1 to: 5197
pF1KSDA0547 5197 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1856 2233 1856
Size: 377 4820
Acc65I G'GTAC_C
Cuts at: 2125 2211 2125
Size: 86 5111
AclI AA'CG_TT
Cuts at: 5037 5037
Size: 5197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1121 1210 1739 2093 3020 3452 4201 4469
Size: 73 89 529 354 927 432 749 268
Cuts at: 4469 1048
Size: 1776
Fragments arranged by size:
1776 927 749 529 432 354 268 89
73
AflII C'TTAA_G
Cuts at: 2169 2169
Size: 5197
AflIII A'CryG_T
Cuts at: 2547 3653 4630 2547
Size: 1106 977 3114
Fragments arranged by size:
3114 1106 977
AgeI A'CCGG_T
Cuts at: 3585 3585
Size: 5197
AleI CACnn'nnGTG
Cuts at: 1087 1087
Size: 5197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 606 606
Size: 5197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2101 2133 2101
Size: 32 5165
AlwNI CAG_nnn'CTG
Cuts at: 1742 2088 2500 4069 1742
Size: 346 412 1569 2870
Fragments arranged by size:
2870 1569 412 346
ApaI G_GGCC'C
Cuts at: 133 133
Size: 5197
ApaLI G'TGCA_C
Cuts at: 3967 3967
Size: 5197
ApoI r'AATT_y
Cuts at: 943 1009 2199 4550 4806 943
Size: 66 1190 2351 256 1334
Fragments arranged by size:
2351 1334 1190 256 66
AscI GG'CGCG_CC
Cuts at: 262 350 262
Size: 88 5109
AseI AT'TA_AT
Cuts at: 20 2038 20
Size: 2018 3179
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5197
AvaI C'yCGr_G
Cuts at: 443 706 1780 2215 443
Size: 263 1074 435 3425
Fragments arranged by size:
3425 1074 435 263
BamHI G'GATC_C
Cuts at: 1694 2220 1694
Size: 526 4671
BanI G'GyrC_C
Cuts at: 643 890 925 2125 2211 2625 2873 2908 643
Size: 247 35 1200 86 414 248 35 2932
Fragments arranged by size:
2932 1200 414 248 247 86 35 35
BanII G_rGCy'C
Cuts at: 133 557 656 1225 1764 1999 2209 3239 133
Size: 424 99 569 539 235 210 1030 2091
Fragments arranged by size:
2091 1030 569 539 424 235 210 99
BbeI G_GCGC'C
Cuts at: 647 894 2629 2877 647
Size: 247 1735 248 2967
Fragments arranged by size:
2967 1735 248 247
BbsI GAAGACnn'nnnn_
Cuts at: 1206 1206
Size: 5197
BbvCI CC'TCA_GC
Cuts at: 190 190
Size: 5197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 811 845 811
Size: 34 5163
BciVI GTATCCnnnnn_n'
Cuts at: 2071 3093 3856 5197 2071
Size: 1022 763 1341 2071
Fragments arranged by size:
2071 1341 1022 763
BclI T'GATC_A
Cuts at: 1973 2715 1973
Size: 742 4455
BglI GCCn_nnn'nGGC
Cuts at: 1340 1340
Size: 5197
BglII A'GATC_T
Cuts at: 377 2710 4749 377
Size: 2333 2039 825
Fragments arranged by size:
2333 2039 825
BlpI GC'TnA_GC
Cuts at: 2307 2307
Size: 5197
Bme1580I G_kGCm'C
Cuts at: 133 2820 2913 3971 133
Size: 2687 93 1058 1359
Fragments arranged by size:
2687 1359 1058 93
BmgBI CAC'GTC
Cuts at: 477 876 477
Size: 399 4798
BmrI ACTGGGnnnn_n'
Cuts at: 1435 1693 2407 2488 2823 4969 1435
Size: 258 714 81 335 2146 1663
Fragments arranged by size:
2146 1663 714 335 258 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1090 3562 1090
Size: 2472 2725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 880 1376 1450 1793 1982 604
Size: 276 496 74 343 189 3819
Fragments arranged by size:
3819 496 343 276 189 74
Bpu10I CC'TnA_GC
Cuts at: 190 190
Size: 5197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 1239 1287 2371 3250 3744 4042 779
Size: 460 48 1084 879 494 298 1934
Fragments arranged by size:
1934 1084 879 494 460 298 48
BsaI GGTCTCn'nnnn_
Cuts at: 1783 1815 4901 1783
Size: 32 3086 2079
Fragments arranged by size:
3086 2079 32
BsaAI yAC'GTr
Cuts at: 222 2187 2548 222
Size: 1965 361 2871
Fragments arranged by size:
2871 1965 361
BsaBI GATnn'nnATC
Cuts at: 6 2709 4748 6
Size: 2703 2039 455
Fragments arranged by size:
2703 2039 455
BsaHI Gr'CG_yC
Cuts at: 511 644 800 891 1374 2626 2874 3578 5074
Size: 133 156 91 483 1252 248 704 1496
Cuts at: 5074 511
Size: 634
Fragments arranged by size:
1496 1252 704 634 483 248 156 133
91
BsaWI w'CCGG_w
Cuts at: 471 2384 2905 3585 3859 4006 4417 471
Size: 1913 521 680 274 147 411 1251
Fragments arranged by size:
1913 1251 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 1060 1090 1955 1985 544
Size: 30 486 30 865 30 3756
Fragments arranged by size:
3756 865 486 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 1030 1807 1941 646
Size: 384 777 134 3902
Fragments arranged by size:
3902 777 384 134
BseYI C'CCAG_C
Cuts at: 746 2176 2620 3378 3957 4453 746
Size: 1430 444 758 579 496 1490
Fragments arranged by size:
1490 1430 758 579 496 444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1262 1728 244
Size: 1018 466 3713
Fragments arranged by size:
3713 1018 466
BsiEI CG_ry'CG
Cuts at: 104 2737 2783 3993 104
Size: 2633 46 1210 1308
Fragments arranged by size:
2633 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1814 1907 1999 2209 2987 3177 3971 1814
Size: 93 92 210 778 190 794 3040
Fragments arranged by size:
3040 794 778 210 190 93 92
BsmI GAATG_Cn'
Cuts at: 1569 1728 1569
Size: 159 5038
BsmBI CGTCTCn'nnnn_
Cuts at: 502 502
Size: 5197
Bsp1286I G_dGCh'C
Cuts at: 133 557 656 1225 1764 1814 1907 1999 2209
Size: 424 99 569 539 50 93 92 210
Cuts at: 2209 2820 2913 2987 3177 3239 3971 133
Size: 611 93 74 190 62 732 1359
Fragments arranged by size:
1359 732 611 569 539 424 210 190
99 93 93 92 74 62 50
BspEI T'CCGG_A
Cuts at: 471 4417 471
Size: 3946 1251
BspHI T'CATG_A
Cuts at: 4373 4373
Size: 5197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2245 2459 2761 3142 2245
Size: 214 302 381 4300
Fragments arranged by size:
4300 381 302 214
BsrBI CCG'CTC
Cuts at: 50 276 3487 3541 5027 5192 50
Size: 226 3211 54 1486 165 55
Fragments arranged by size:
3211 1486 226 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3107 3107
Size: 5197
BsrFI r'CCGG_y
Cuts at: 977 3193 3374 3585 977
Size: 2216 181 211 2589
Fragments arranged by size:
2589 2216 211 181
BssHII G'CGCG_C
Cuts at: 211 262 327 350 371 211
Size: 51 65 23 21 5037
Fragments arranged by size:
5037 65 51 23 21
BssSI C'ACGA_G
Cuts at: 3466 3826 3466
Size: 360 4837
BstAPI GCAn_nnn'nTGC
Cuts at: 324 2572 324
Size: 2248 2949
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5197
BstYI r'GATC_y
Cuts at: 1 377 1694 1934 2220 2687 2710 3045 3291
Size: 376 1317 240 286 467 23 335 246
Cuts at: 3291 4305 4391 4403 4749 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1317 1014 467 449 376 346 335 286
246 240 86 23 12
BtgI C'CryG_G
Cuts at: 125 428 647 722 929 2029 125
Size: 303 219 75 207 1100 3293
Fragments arranged by size:
3293 1100 303 219 207 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 717 3145 3326 3489 717
Size: 2428 181 163 2425
Fragments arranged by size:
2428 2425 181 163
BtsI GCAGTG_nn'
Cuts at: 318 951 1272 2438 318
Size: 633 321 1166 3077
Fragments arranged by size:
3077 1166 633 321
DraI TTT'AAA
Cuts at: 2194 2194
Size: 5197
DraIII CAC_nnn'GTG
Cuts at: 324 1556 324
Size: 1232 3965
DrdI GACnn_nn'nnGTC
Cuts at: 2901 3761 2901
Size: 860 4337
EaeI y'GGCC_r
Cuts at: 206 683 807 1200 1533 2032 2780 2954 3345
Size: 477 124 393 333 499 748 174 391
Cuts at: 3345 3372 4719 206
Size: 27 1347 684
Fragments arranged by size:
1347 748 684 499 477 393 391 333
174 124 27
EagI C'GGCC_G
Cuts at: 2780 2780
Size: 5197
EarI CTCTTCn'nnn_
Cuts at: 1186 2069 3218 3428 1186
Size: 883 1149 210 2955
Fragments arranged by size:
2955 1149 883 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3403 3715 3861 5007 3403
Size: 312 146 1146 3593
Fragments arranged by size:
3593 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1997 2207 1997
Size: 210 4987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 880 1048 1121 1210 1376 1450 1739 1793
Size: 276 168 73 89 166 74 289 54
Cuts at: 1793 1982 2093 3020 3452 4201 4469 604
Size: 189 111 927 432 749 268 1332
Fragments arranged by size:
1332 927 749 432 289 276 268 189
168 166 111 89 74 73 54
EcoNI CCTnn'n_nnAGG
Cuts at: 685 685
Size: 5197
EcoO109I rG'GnC_Cy
Cuts at: 130 228 636 1179 1506 2334 130
Size: 98 408 543 327 828 2993
Fragments arranged by size:
2993 828 543 408 327 98
EcoRI G'AATT_C
Cuts at: 943 2199 943
Size: 1256 3941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3214 3214
Size: 5197
FspI TGC'GCA
Cuts at: 2976 2976
Size: 5197
HaeII r_GCGC'y
Cuts at: 188 395 647 894 2629 2877 3901 4880 188
Size: 207 252 247 1735 248 1024 979 505
Fragments arranged by size:
1735 1024 979 505 252 248 247 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 369 401 544 576 649 681 777 809 1060
Size: 32 143 32 73 32 96 32 251
Cuts at: 1060 1092 1300 1332 3037 3067 3069 3099 369
Size: 32 208 32 1705 30 2 30 2467
Fragments arranged by size:
2467 1705 251 208 143 96 73 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 530 2234 530
Size: 1704 3493
HpaI GTT'AAC
Cuts at: 530 530
Size: 5197
KasI G'GCGC_C
Cuts at: 643 890 2625 2873 643
Size: 247 1735 248 2967
Fragments arranged by size:
2967 1735 248 247
KpnI G_GTAC'C
Cuts at: 2129 2215 2129
Size: 86 5111
MluI A'CGCG_T
Cuts at: 4630 4630
Size: 5197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1908 3868 4052 1908
Size: 1960 184 3053
Fragments arranged by size:
3053 1960 184
MscI TGG'CCA
Cuts at: 809 1202 2034 2956 809
Size: 393 832 922 3050
Fragments arranged by size:
3050 922 832 393
MslI CAynn'nnrTG
Cuts at: 1087 3184 3311 1087
Size: 2097 127 2973
Fragments arranged by size:
2973 2097 127
MspA1I CmG'CkG
Cuts at: 430 649 724 888 2306 2620 2980 3995 4240
Size: 219 75 164 1418 314 360 1015 245
Cuts at: 4240 430
Size: 1387
Fragments arranged by size:
1418 1387 1015 360 314 245 219 164
75
NaeI GCC'GGC
Cuts at: 979 3376 979
Size: 2397 2800
NarI GG'CG_CC
Cuts at: 644 891 2626 2874 644
Size: 247 1735 248 2967
Fragments arranged by size:
2967 1735 248 247
NcoI C'CATG_G
Cuts at: 125 2029 125
Size: 1904 3293
NdeI CA'TA_TG
Cuts at: 2022 2022
Size: 5197
NgoMIV G'CCGG_C
Cuts at: 977 3374 977
Size: 2397 2800
NruI TCG'CGA
Cuts at: 3558 3558
Size: 5197
NspI r_CATG'y
Cuts at: 2248 2748 3657 2248
Size: 500 909 3788
Fragments arranged by size:
3788 909 500
PasI CC'CwG_GG
Cuts at: 639 639
Size: 5197
PciI A'CATG_T
Cuts at: 3653 3653
Size: 5197
PflMI CCAn_nnn'nTGG
Cuts at: 627 627
Size: 5197
PfoI T'CCnGG_A
Cuts at: 1959 1959
Size: 5197
PmeI GTTT'AAAC
Cuts at: 2194 2194
Size: 5197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4336 4368 4336
Size: 32 5165
PpuMI rG'GwC_Cy
Cuts at: 228 1179 1506 228
Size: 951 327 3919
Fragments arranged by size:
3919 951 327
PspOMI G'GGCC_C
Cuts at: 129 129
Size: 5197
PspXI vC'TCGA_Gb
Cuts at: 706 706
Size: 5197
PstI C_TGCA'G
Cuts at: 453 2242 2927 453
Size: 1789 685 2723
Fragments arranged by size:
2723 1789 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5197
PvuII CAG'CTG
Cuts at: 2620 2980 2620
Size: 360 4837
RsrII CG'GwC_CG
Cuts at: 3390 3390
Size: 5197
SacI G_AGCT'C
Cuts at: 1999 2209 1999
Size: 210 4987
SacII CC_GC'GG
Cuts at: 431 650 725 431
Size: 219 75 4903
Fragments arranged by size:
4903 219 75
SalI G'TCGA_C
Cuts at: 2232 2232
Size: 5197
SapI GCTCTTCn'nnn_
Cuts at: 2069 3218 3428 2069
Size: 1149 210 3838
Fragments arranged by size:
3838 1149 210
SbfI CC_TGCA'GG
Cuts at: 2242 2242
Size: 5197
ScaI AGT'ACT
Cuts at: 4714 4714
Size: 5197
SfcI C'TryA_G
Cuts at: 33 449 1985 2238 2923 3918 4109 33
Size: 416 1536 253 685 995 191 1121
Fragments arranged by size:
1536 1121 995 685 416 253 191
SfoI GGC'GCC
Cuts at: 645 892 2627 2875 645
Size: 247 1735 248 2967
Fragments arranged by size:
2967 1735 248 247
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5197
SgrAI Cr'CCGG_yG
Cuts at: 977 977
Size: 5197
SmaI CCC'GGG
Cuts at: 445 2217 445
Size: 1772 3425
SmlI C'TyrA_G
Cuts at: 706 794 1218 1302 2169 2350 3265 3759 4021
Size: 88 424 84 867 181 915 494 262
Cuts at: 4021 706
Size: 1882
Fragments arranged by size:
1882 915 867 494 424 262 181 88
84
SnaBI TAC'GTA
Cuts at: 2187 2187
Size: 5197
SpeI A'CTAG_T
Cuts at: 67 2056 67
Size: 1989 3208
SphI G_CATG'C
Cuts at: 2248 2748 2248
Size: 500 4697
SrfI GCCC'GGGC
Cuts at: 445 445
Size: 5197
SspI AAT'ATT
Cuts at: 993 1606 993
Size: 613 4584
StuI AGG'CCT
Cuts at: 792 792
Size: 5197
StyI C'CwwG_G
Cuts at: 125 1454 1879 2029 2094 2329 2683 125
Size: 1329 425 150 65 235 354 2639
Fragments arranged by size:
2639 1329 425 354 235 150 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 684 2778 2911 3370 3581 684
Size: 2094 133 459 211 2300
Fragments arranged by size:
2300 2094 459 211 133
TatI w'GTAC_w
Cuts at: 1644 4712 1644
Size: 3068 2129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1955 1987 4714 4746 1955
Size: 32 2727 32 2406
Fragments arranged by size:
2727 2406 32 32
Tth111I GACn'n_nGTC
Cuts at: 2992 2992
Size: 5197
XbaI T'CTAG_A
Cuts at: 2226 2226
Size: 5197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 2690 678
Size: 2012 3185
XhoI C'TCGA_G
Cuts at: 706 706
Size: 5197
XmaI C'CCGG_G
Cuts at: 443 2215 443
Size: 1772 3425
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AvrII BaeI BfrBI BmtI
BsiWI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII MfeI NheI NotI NsiI
PacI PmlI PshAI PsiI PsrI SanDI SexAI SfiI
SwaI XmnI ZraI