Restriction map for
pF1KSDA0525
(Circular) (Six-base) MAPSORT of: pF1KSDA0525.seq Check: 4353 from: 1 to: 5983
pF1KSDA0525 5983 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 431 431
Size: 5983
AccI GT'mk_AC
Cuts at: 1384 2420 3019 1384
Size: 1036 599 4348
Fragments arranged by size:
4348 1036 599
Acc65I G'GTAC_C
Cuts at: 1805 2997 1805
Size: 1192 4791
AclI AA'CG_TT
Cuts at: 5823 5823
Size: 5983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 830 2793 2972 3806 4238 4987 5255 251
Size: 579 1963 179 834 432 749 268 979
Fragments arranged by size:
1963 979 834 749 579 432 268 179
AfeI AGC'GCT
Cuts at: 1485 1485
Size: 5983
AflIII A'CryG_T
Cuts at: 3333 4439 5416 3333
Size: 1106 977 3900
Fragments arranged by size:
3900 1106 977
AgeI A'CCGG_T
Cuts at: 4371 4371
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 1068 1068
Size: 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1596 1596
Size: 5983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 2306
Size: 32 5951
AlwNI CAG_nnn'CTG
Cuts at: 492 1174 3286 4855 492
Size: 682 2112 1569 1620
Fragments arranged by size:
2112 1620 1569 682
ApaI G_GGCC'C
Cuts at: 2454 2454
Size: 5983
ApaLI G'TGCA_C
Cuts at: 2335 4753 2335
Size: 2418 3565
ApoI r'AATT_y
Cuts at: 997 1264 1365 1921 2530 2627 2898 2985 5336
Size: 267 101 556 609 97 271 87 2351
Cuts at: 5336 5592 997
Size: 256 1388
Fragments arranged by size:
2351 1388 609 556 271 267 256 101
97 87
AseI AT'TA_AT
Cuts at: 20 20
Size: 5983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5983
AvaI C'yCGr_G
Cuts at: 532 3001 532
Size: 2469 3514
BamHI G'GATC_C
Cuts at: 3006 3006
Size: 5983
BanI G'GyrC_C
Cuts at: 761 1789 1805 2997 3411 3659 3694 761
Size: 1028 16 1192 414 248 35 3050
Fragments arranged by size:
3050 1192 1028 414 248 35 16
BanII G_rGCy'C
Cuts at: 539 554 1783 2287 2454 2995 4025 539
Size: 15 1229 504 167 541 1030 2497
Fragments arranged by size:
2497 1229 1030 541 504 167 15
BbeI G_GCGC'C
Cuts at: 1793 3415 3663 1793
Size: 1622 248 4113
Fragments arranged by size:
4113 1622 248
BbsI GAAGACnn'nnnn_
Cuts at: 830 1133 830
Size: 303 5680
BciVI GTATCCnnnnn_n'
Cuts at: 644 1037 2653 2962 3879 4642 5983 644
Size: 393 1616 309 917 763 1341 644
Fragments arranged by size:
1616 1341 917 763 644 393 309
BclI T'GATC_A
Cuts at: 3501 3501
Size: 5983
BfrBI ATG'CAT
Cuts at: 2053 2053
Size: 5983
BglII A'GATC_T
Cuts at: 1044 3496 5535 1044
Size: 2452 2039 1492
Fragments arranged by size:
2452 2039 1492
BlpI GC'TnA_GC
Cuts at: 3093 3093
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 2339 2454 3606 3699 4757 2339
Size: 115 1152 93 1058 3565
Fragments arranged by size:
3565 1152 1058 115 93
BmgBI CAC'GTC
Cuts at: 2424 2424
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 298 1098 1811 2647 3193 3274 3609 5755 298
Size: 800 713 836 546 81 335 2146 526
Fragments arranged by size:
2146 836 800 713 546 526 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4348 4348
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 1504 479
Size: 1025 4958
Bpu10I CC'TnA_GC
Cuts at: 1468 2245 1468
Size: 777 5206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2427 3157 4036 4530 4828 2427
Size: 730 879 494 298 3582
Fragments arranged by size:
3582 879 730 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5687 5687
Size: 5983
BsaAI yAC'GTr
Cuts at: 2973 3334 2973
Size: 361 5622
BsaBI GATnn'nnATC
Cuts at: 6 3495 5534 6
Size: 3489 2039 455
Fragments arranged by size:
3489 2039 455
BsaHI Gr'CG_yC
Cuts at: 1790 3412 3660 4364 5860 1790
Size: 1622 248 704 1496 1913
Fragments arranged by size:
1913 1622 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3170 3691 4371 4645 4792 5203 3170
Size: 521 680 274 147 411 3950
Fragments arranged by size:
3950 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 908 938 1264 1294 1502 1532 2306 2336 908
Size: 30 326 30 208 30 774 30 4555
Fragments arranged by size:
4555 774 326 208 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2314 2314
Size: 5983
BseYI C'CCAG_C
Cuts at: 2468 3406 4164 4743 5239 2468
Size: 938 758 579 496 3212
Fragments arranged by size:
3212 938 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 3523 3569 4779 104
Size: 3419 46 1210 1308
Fragments arranged by size:
3419 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 229 856 1769 1892 2339 2461 2995 3773 3963
Size: 627 913 123 447 122 534 778 190
Cuts at: 3963 4757 229
Size: 794 1455
Fragments arranged by size:
1455 913 794 778 627 534 447 190
123 122
Bsp1286I G_dGCh'C
Cuts at: 229 539 554 856 1769 1783 1892 2287 2339
Size: 310 15 302 913 14 109 395 52
Cuts at: 2339 2454 2461 2995 3606 3699 3773 3963 4025
Size: 115 7 534 611 93 74 190 62
Cuts at: 4025 4757 229
Size: 732 1455
Fragments arranged by size:
1455 913 732 611 534 395 310 302
190 115 109 93 74 62 52 15
14 7
BspEI T'CCGG_A
Cuts at: 5203 5203
Size: 5983
BspHI T'CATG_A
Cuts at: 5159 5159
Size: 5983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 477 723 3031 3245 3547 3928 431
Size: 46 246 2308 214 302 381 2486
Fragments arranged by size:
2486 2308 381 302 246 214 46
BsrBI CCG'CTC
Cuts at: 50 4273 4327 5813 5978 50
Size: 4223 54 1486 165 55
Fragments arranged by size:
4223 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1352 3893 1352
Size: 2541 3442
BsrFI r'CCGG_y
Cuts at: 3979 4160 4371 3979
Size: 181 211 5591
Fragments arranged by size:
5591 211 181
BsrGI T'GTAC_A
Cuts at: 1509 1749 2706 1509
Size: 240 957 4786
Fragments arranged by size:
4786 957 240
BssSI C'ACGA_G
Cuts at: 4252 4612 4252
Size: 360 5623
BstAPI GCAn_nnn'nTGC
Cuts at: 967 976 2304 2440 3358 967
Size: 9 1328 136 918 3592
Fragments arranged by size:
3592 1328 918 136 9
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 269 1204 1269 2672 269
Size: 935 65 1403 3580
Fragments arranged by size:
3580 1403 935 65
BstYI r'GATC_y
Cuts at: 1 1044 1409 3006 3473 3496 3831 4077 5091
Size: 1043 365 1597 467 23 335 246 1014
Cuts at: 5091 5177 5189 5535 1
Size: 86 12 346 449
Fragments arranged by size:
1597 1043 1014 467 449 365 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 445 506 445
Size: 61 5922
BtgI C'CryG_G
Cuts at: 125 603 125
Size: 478 5505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3931 4112 4275 3931
Size: 181 163 5639
Fragments arranged by size:
5639 181 163
BtsI GCAGTG_nn'
Cuts at: 520 677 1699 2441 3224 520
Size: 157 1022 742 783 3279
Fragments arranged by size:
3279 1022 783 742 157
ClaI AT'CG_AT
Cuts at: 1860 1860
Size: 5983
DraI TTT'AAA
Cuts at: 1364 1497 1997 2980 1364
Size: 133 500 983 4367
Fragments arranged by size:
4367 983 500 133
DraIII CAC_nnn'GTG
Cuts at: 1380 1380
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 3687 4547 3687
Size: 860 5123
EaeI y'GGCC_r
Cuts at: 3566 3740 4131 4158 5505 3566
Size: 174 391 27 1347 4044
Fragments arranged by size:
4044 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3566 3566
Size: 5983
EarI CTCTTCn'nnn_
Cuts at: 744 995 1898 2786 4004 4214 744
Size: 251 903 888 1218 210 2513
Fragments arranged by size:
2513 1218 903 888 251 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4189 4501 4647 5793 4189
Size: 312 146 1146 4379
Fragments arranged by size:
4379 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2993 2993
Size: 5983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 479 830 1504 2793 2972 3806 4238 4987
Size: 228 351 674 1289 179 834 432 749
Cuts at: 4987 5255 251
Size: 268 979
Fragments arranged by size:
1289 979 834 749 674 432 351 268
228 179
EcoNI CCTnn'n_nnAGG
Cuts at: 448 448
Size: 5983
EcoO109I rG'GnC_Cy
Cuts at: 489 1554 2250 2450 3120 489
Size: 1065 696 200 670 3352
Fragments arranged by size:
3352 1065 696 670 200
EcoRI G'AATT_C
Cuts at: 2985 2985
Size: 5983
EcoRV GAT'ATC
Cuts at: 431 431
Size: 5983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 5983
FspI TGC'GCA
Cuts at: 3762 3762
Size: 5983
HaeII r_GCGC'y
Cuts at: 1487 1793 3415 3663 4687 5666 1487
Size: 306 1622 248 1024 979 1804
Fragments arranged by size:
1804 1622 1024 979 306 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 843 874 875 906 1054 1086 1264
Size: 32 357 31 1 31 148 32 178
Cuts at: 1264 1296 2269 2301 3823 3853 3855 3885 454
Size: 32 973 32 1522 30 2 30 2552
Fragments arranged by size:
2552 1522 973 357 178 148 32 32
32 32 31 31 30 30 2 1
HincII GTy'rAC
Cuts at: 1630 2421 3020 1630
Size: 791 599 4593
Fragments arranged by size:
4593 791 599
HindIII A'AGCT_T
Cuts at: 1156 2563 2586 2935 1156
Size: 1407 23 349 4204
Fragments arranged by size:
4204 1407 349 23
KasI G'GCGC_C
Cuts at: 1789 3411 3659 1789
Size: 1622 248 4113
Fragments arranged by size:
4113 1622 248
KpnI G_GTAC'C
Cuts at: 1809 3001 1809
Size: 1192 4791
MfeI C'AATT_G
Cuts at: 2858 2858
Size: 5983
MluI A'CGCG_T
Cuts at: 5416 5416
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1293 1871 2866 2874 4654 4838 1293
Size: 578 995 8 1780 184 2438
Fragments arranged by size:
2438 1780 995 578 184 8
MscI TGG'CCA
Cuts at: 3742 3742
Size: 5983
MslI CAynn'nnrTG
Cuts at: 833 1168 1732 2749 3970 4097 833
Size: 335 564 1017 1221 127 2719
Fragments arranged by size:
2719 1221 1017 564 335 127
MspA1I CmG'CkG
Cuts at: 3092 3406 3766 4781 5026 3092
Size: 314 360 1015 245 4049
Fragments arranged by size:
4049 1015 360 314 245
NaeI GCC'GGC
Cuts at: 4162 4162
Size: 5983
NarI GG'CG_CC
Cuts at: 1790 3412 3660 1790
Size: 1622 248 4113
Fragments arranged by size:
4113 1622 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5983
NgoMIV G'CCGG_C
Cuts at: 4160 4160
Size: 5983
NruI TCG'CGA
Cuts at: 4344 4344
Size: 5983
NsiI A_TGCA'T
Cuts at: 2055 2055
Size: 5983
NspI r_CATG'y
Cuts at: 1819 3034 3534 4443 1819
Size: 1215 500 909 3359
Fragments arranged by size:
3359 1215 909 500
PciI A'CATG_T
Cuts at: 4439 4439
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1188 1188
Size: 5983
PfoI T'CCnGG_A
Cuts at: 491 1412 491
Size: 921 5062
PmeI GTTT'AAAC
Cuts at: 2980 2980
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2306 2338 5122 5154 2306
Size: 32 2784 32 3135
Fragments arranged by size:
3135 2784 32 32
PpuMI rG'GwC_Cy
Cuts at: 489 1554 2250 489
Size: 1065 696 4222
Fragments arranged by size:
4222 1065 696
PspOMI G'GGCC_C
Cuts at: 2450 2450
Size: 5983
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 635 667 635
Size: 32 5951
PstI C_TGCA'G
Cuts at: 428 488 684 1706 2545 3028 3713 428
Size: 60 196 1022 839 483 685 2698
Fragments arranged by size:
2698 1022 839 685 483 196 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5983
PvuII CAG'CTG
Cuts at: 3406 3766 3406
Size: 360 5623
RsrII CG'GwC_CG
Cuts at: 4176 4176
Size: 5983
SacI G_AGCT'C
Cuts at: 2995 2995
Size: 5983
SalI G'TCGA_C
Cuts at: 2419 3018 2419
Size: 599 5384
SapI GCTCTTCn'nnn_
Cuts at: 4004 4214 4004
Size: 210 5773
SbfI CC_TGCA'GG
Cuts at: 488 3028 488
Size: 2540 3443
ScaI AGT'ACT
Cuts at: 2511 5500 2511
Size: 2989 2994
SexAI A'CCwGG_T
Cuts at: 1211 1211
Size: 5983
SfcI C'TryA_G
Cuts at: 33 424 484 624 680 1586 1702 2541 3024
Size: 391 60 140 56 906 116 839 483
Cuts at: 3024 3709 4704 4895 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 906 839 685 483 391 191
140 116 60 56
SfoI GGC'GCC
Cuts at: 1791 3413 3661 1791
Size: 1622 248 4113
Fragments arranged by size:
4113 1622 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5983
SmaI CCC'GGG
Cuts at: 3003 3003
Size: 5983
SmlI C'TyrA_G
Cuts at: 2406 3136 4051 4545 4807 2406
Size: 730 915 494 262 3582
Fragments arranged by size:
3582 915 730 494 262
SnaBI TAC'GTA
Cuts at: 2973 2973
Size: 5983
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5983
SphI G_CATG'C
Cuts at: 3034 3534 3034
Size: 500 5483
StuI AGG'CCT
Cuts at: 2095 2227 2095
Size: 132 5851
StyI C'CwwG_G
Cuts at: 125 268 632 757 2096 2429 3115 3469 125
Size: 143 364 125 1339 333 686 354 2639
Fragments arranged by size:
2639 1339 686 364 354 333 143 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3564 3697 4156 4367 3564
Size: 133 459 211 5180
Fragments arranged by size:
5180 459 211 133
TatI w'GTAC_w
Cuts at: 1509 1749 2111 2509 2706 5498 1509
Size: 240 362 398 197 2792 1994
Fragments arranged by size:
2792 1994 398 362 240 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5500 5532 5500
Size: 32 5951
Tth111I GACn'n_nGTC
Cuts at: 3778 3778
Size: 5983
XbaI T'CTAG_A
Cuts at: 993 1621 3012 993
Size: 628 1391 3964
Fragments arranged by size:
3964 1391 628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 783 1201 2742 3476 783
Size: 418 1541 734 3290
Fragments arranged by size:
3290 1541 734 418
XmaI C'CCGG_G
Cuts at: 3001 3001
Size: 5983
XmnI GAAnn'nnTTC
Cuts at: 2564 2564
Size: 5983
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AscI AvrII BaeI BbvCI BcgI
BglI BmtI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I CspCI FseI FspAI HpaI NdeI NheI NotI
PacI PasI PmlI PshAI PsiI PspXI SacII SanDI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI