Restriction map for
pF1KSDA0475
(Circular) (Six-base) MAPSORT of: pF1KSDA0475.seq Check: 1164 from: 1 to: 4366
pF1KSDA0475 4366 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1402 1402
Size: 4366
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4366
AclI AA'CG_TT
Cuts at: 4206 4206
Size: 4366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 2189 2621 3370 3638 380
Size: 1809 432 749 268 1108
Fragments arranged by size:
1809 1108 749 432 268
AflIII A'CryG_T
Cuts at: 1716 2822 3799 1716
Size: 1106 977 2283
Fragments arranged by size:
2283 1106 977
AgeI A'CCGG_T
Cuts at: 2754 2754
Size: 4366
AlwNI CAG_nnn'CTG
Cuts at: 1669 3238 1669
Size: 1569 2797
ApaLI G'TGCA_C
Cuts at: 1290 3136 1290
Size: 1846 2520
ApoI r'AATT_y
Cuts at: 1368 3719 3975 1368
Size: 2351 256 1759
Fragments arranged by size:
2351 1759 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4366
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4366
AvaI C'yCGr_G
Cuts at: 344 1384 344
Size: 1040 3326
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4366
BanI G'GyrC_C
Cuts at: 282 1380 1794 2042 2077 282
Size: 1098 414 248 35 2571
Fragments arranged by size:
2571 1098 414 248 35
BanII G_rGCy'C
Cuts at: 612 1378 2408 612
Size: 766 1030 2570
Fragments arranged by size:
2570 1030 766
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4118
BbsI GAAGACnn'nnnn_
Cuts at: 1334 1334
Size: 4366
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3025 4366 2262
Size: 763 1341 2262
Fragments arranged by size:
2262 1341 763
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4366
BfrBI ATG'CAT
Cuts at: 390 390
Size: 4366
BglII A'GATC_T
Cuts at: 1879 3918 1879
Size: 2039 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4366
Bme1580I G_kGCm'C
Cuts at: 1294 1989 2082 3140 1294
Size: 695 93 1058 2520
Fragments arranged by size:
2520 1058 695 93
BmrI ACTGGGnnnn_n'
Cuts at: 324 1213 1576 1657 1992 4138 324
Size: 889 363 81 335 2146 552
Fragments arranged by size:
2146 889 552 363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4366
Bpu10I CC'TnA_GC
Cuts at: 497 497
Size: 4366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 2913 3211 1540
Size: 879 494 298 2695
Fragments arranged by size:
2695 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 506 4070 506
Size: 3564 802
BsaAI yAC'GTr
Cuts at: 1356 1717 1356
Size: 361 4005
BsaBI GATnn'nnATC
Cuts at: 6 1235 1878 3917 6
Size: 1229 643 2039 455
Fragments arranged by size:
2039 1229 643 455
BsaHI Gr'CG_yC
Cuts at: 1244 1795 2043 2747 4243 1244
Size: 551 248 704 1496 1367
Fragments arranged by size:
1496 1367 704 551 248
BsaWI w'CCGG_w
Cuts at: 1553 2074 2754 3028 3175 3586 1553
Size: 521 680 274 147 411 2333
Fragments arranged by size:
2333 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1198 1228 1198
Size: 30 4336
BseYI C'CCAG_C
Cuts at: 375 1789 2547 3126 3622 375
Size: 1414 758 579 496 1119
Fragments arranged by size:
1414 1119 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3162 104
Size: 1802 46 1210 1308
Fragments arranged by size:
1802 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 685 1226 1294 1378 2156 2346 3140 685
Size: 541 68 84 778 190 794 1911
Fragments arranged by size:
1911 794 778 541 190 84 68
BsmI GAATG_Cn'
Cuts at: 1094 1094
Size: 4366
Bsp1286I G_dGCh'C
Cuts at: 612 685 1226 1294 1378 1989 2082 2156 2346
Size: 73 541 68 84 611 93 74 190
Cuts at: 2346 2408 3140 612
Size: 62 732 1838
Fragments arranged by size:
1838 732 611 541 190 93 84 74
73 68 62
BspEI T'CCGG_A
Cuts at: 3586 3586
Size: 4366
BspHI T'CATG_A
Cuts at: 3542 3542
Size: 4366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1414 1628 1930 2311 1414
Size: 214 302 381 3469
Fragments arranged by size:
3469 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4196 4361 50
Size: 2606 54 1486 165 55
Fragments arranged by size:
2606 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 990 2276 990
Size: 1286 3080
BsrFI r'CCGG_y
Cuts at: 2362 2543 2754 2362
Size: 181 211 3974
Fragments arranged by size:
3974 211 181
BssSI C'ACGA_G
Cuts at: 2635 2995 2635
Size: 360 4006
BstAPI GCAn_nnn'nTGC
Cuts at: 1741 1741
Size: 4366
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4366
BstXI CCAn_nnnn'nTGG
Cuts at: 809 809
Size: 4366
BstYI r'GATC_y
Cuts at: 1 781 1389 1856 1879 2214 2460 3474 3560
Size: 780 608 467 23 335 246 1014 86
Cuts at: 3560 3572 3918 1
Size: 12 346 449
Fragments arranged by size:
1014 780 608 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 395 835 1196 1247 395
Size: 440 361 51 3514
Fragments arranged by size:
3514 440 361 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4022
Fragments arranged by size:
4022 181 163
BtsI GCAGTG_nn'
Cuts at: 1294 1607 1294
Size: 313 4053
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4366
DrdI GACnn_nn'nnGTC
Cuts at: 961 2070 2930 961
Size: 1109 860 2397
Fragments arranged by size:
2397 1109 860
EaeI y'GGCC_r
Cuts at: 398 866 1274 1949 2123 2514 2541 3888 398
Size: 468 408 675 174 391 27 1347 876
Fragments arranged by size:
1347 876 675 468 408 391 174 27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4366
EarI CTCTTCn'nnn_
Cuts at: 356 2387 2597 356
Size: 2031 210 2125
Fragments arranged by size:
2125 2031 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2572 2884 3030 4176 2572
Size: 312 146 1146 2762
Fragments arranged by size:
2762 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 2189 2621 3370 3638 380
Size: 1809 432 749 268 1108
Fragments arranged by size:
1809 1108 749 432 268
EcoO109I rG'GnC_Cy
Cuts at: 242 1503 242
Size: 1261 3105
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1200 2383 1200
Size: 1183 3183
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4366
HaeII r_GCGC'y
Cuts at: 1031 1798 2046 3070 4049 1031
Size: 767 248 1024 979 1348
Fragments arranged by size:
1348 1024 979 767 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1198 1230 2206 2236 2238 2268 1198
Size: 32 976 30 2 30 3296
Fragments arranged by size:
3296 976 32 30 30 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4366
HindIII A'AGCT_T
Cuts at: 1062 1062
Size: 4366
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4118
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4366
MluI A'CGCG_T
Cuts at: 3799 3799
Size: 4366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3037 3221 3037
Size: 184 4182
MscI TGG'CCA
Cuts at: 400 868 1276 2125 400
Size: 468 408 849 2641
Fragments arranged by size:
2641 849 468 408
MslI CAynn'nnrTG
Cuts at: 1001 1129 2353 2480 1001
Size: 128 1224 127 2887
Fragments arranged by size:
2887 1224 128 127
MspA1I CmG'CkG
Cuts at: 220 447 675 961 1257 1475 1789 2149 3164
Size: 227 228 286 296 218 314 360 1015
Cuts at: 3164 3409 220
Size: 245 1177
Fragments arranged by size:
1177 1015 360 314 296 286 245 228
227 218
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 4366
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4118
NcoI C'CATG_G
Cuts at: 395 835 1196 395
Size: 440 361 3565
Fragments arranged by size:
3565 440 361
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 4366
NruI TCG'CGA
Cuts at: 2727 2727
Size: 4366
NsiI A_TGCA'T
Cuts at: 392 392
Size: 4366
NspI r_CATG'y
Cuts at: 394 1417 1917 2826 394
Size: 1023 500 909 1934
Fragments arranged by size:
1934 1023 909 500
PasI CC'CwG_GG
Cuts at: 314 314
Size: 4366
PciI A'CATG_T
Cuts at: 2822 2822
Size: 4366
PflMI CCAn_nnn'nTGG
Cuts at: 336 522 336
Size: 186 4180
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3505 3537 3505
Size: 32 4334
PsiI TTA'TAA
Cuts at: 434 434
Size: 4366
PstI C_TGCA'G
Cuts at: 821 1411 2096 821
Size: 590 685 3091
Fragments arranged by size:
3091 685 590
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4366
PvuII CAG'CTG
Cuts at: 220 447 675 961 1789 2149 220
Size: 227 228 286 828 360 2437
Fragments arranged by size:
2437 828 360 286 228 227
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4366
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4366
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4366
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4156
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4366
ScaI AGT'ACT
Cuts at: 1319 3883 1319
Size: 2564 1802
SfcI C'TryA_G
Cuts at: 33 695 817 1407 2092 3087 3278 33
Size: 662 122 590 685 995 191 1121
Fragments arranged by size:
1121 995 685 662 590 191 122
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4118
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4366
SmaI CCC'GGG
Cuts at: 346 1386 346
Size: 1040 3326
SmlI C'TyrA_G
Cuts at: 1519 2434 2928 3190 1519
Size: 915 494 262 2695
Fragments arranged by size:
2695 915 494 262
SnaBI TAC'GTA
Cuts at: 1356 1356
Size: 4366
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4366
SphI G_CATG'C
Cuts at: 394 1417 1917 394
Size: 1023 500 2843
Fragments arranged by size:
2843 1023 500
StyI C'CwwG_G
Cuts at: 395 614 835 1196 1498 1852 395
Size: 219 221 361 302 354 2909
Fragments arranged by size:
2909 361 354 302 221 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 300 324 848 1947 2080 2539 2750 300
Size: 24 524 1099 133 459 211 1916
Fragments arranged by size:
1916 1099 524 459 211 133 24
TatI w'GTAC_w
Cuts at: 1317 3881 1317
Size: 2564 1802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3883 3915 3883
Size: 32 4334
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4366
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1859 1859
Size: 4366
XmaI C'CCGG_G
Cuts at: 344 1384 344
Size: 1040 3326
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PasI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI PacI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI