Restriction map for
pF1KSDA0470
(Circular) (Six-base) MAPSORT of: pF1KSDA0470.seq Check: 9107 from: 1 to: 7519
pF1KSDA0470 7519 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 277 1889 2891 4449 4555 277
Size: 1612 1002 1558 106 3241
Fragments arranged by size:
3241 1612 1558 1002 106
Acc65I G'GTAC_C
Cuts at: 4533 4533
Size: 7519
AclI AA'CG_TT
Cuts at: 1575 7359 1575
Size: 5784 1735
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 466 485 590 611 746 1846 2759 3149
Size: 2 19 105 21 135 1100 913 390
Cuts at: 3149 3230 3353 3488 5342 5774 6523 6791 464
Size: 81 123 135 1854 432 749 268 1192
Fragments arranged by size:
1854 1192 1100 913 749 432 390 268
135 135 123 105 81 21 19 2
AfeI AGC'GCT
Cuts at: 3534 3534
Size: 7519
AflII C'TTAA_G
Cuts at: 454 1323 454
Size: 869 6650
AflIII A'CryG_T
Cuts at: 4869 5975 6952 4869
Size: 1106 977 5436
Fragments arranged by size:
5436 1106 977
AgeI A'CCGG_T
Cuts at: 5907 5907
Size: 7519
AleI CACnn'nnGTG
Cuts at: 2824 2824
Size: 7519
AlwNI CAG_nnn'CTG
Cuts at: 562 1292 2629 3715 4191 4822 6391 562
Size: 730 1337 1086 476 631 1569 1690
Fragments arranged by size:
1690 1569 1337 1086 730 631 476
ApaLI G'TGCA_C
Cuts at: 6289 6289
Size: 7519
ApoI r'AATT_y
Cuts at: 865 973 1645 2202 2273 2851 3418 4414 4521
Size: 108 672 557 71 578 567 996 107
Cuts at: 4521 6872 7128 865
Size: 2351 256 1256
Fragments arranged by size:
2351 1256 996 672 578 567 557 256
108 107 71
AseI AT'TA_AT
Cuts at: 20 20
Size: 7519
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7519
AvaI C'yCGr_G
Cuts at: 3662 4341 4537 3662
Size: 679 196 6644
Fragments arranged by size:
6644 679 196
BamHI G'GATC_C
Cuts at: 2478 4542 2478
Size: 2064 5455
BanI G'GyrC_C
Cuts at: 1107 4533 4947 5195 5230 1107
Size: 3426 414 248 35 3396
Fragments arranged by size:
3426 3396 414 248 35
BanII G_rGCy'C
Cuts at: 221 1284 1479 4383 4531 5561 221
Size: 1063 195 2904 148 1030 2179
Fragments arranged by size:
2904 2179 1063 1030 195 148
BbeI G_GCGC'C
Cuts at: 4951 5199 4951
Size: 248 7271
BbsI GAAGACnn'nnnn_
Cuts at: 1052 2117 3596 1052
Size: 1065 1479 4975
Fragments arranged by size:
4975 1479 1065
BbvCI CC'TCA_GC
Cuts at: 2267 3006 3486 2267
Size: 739 480 6300
Fragments arranged by size:
6300 739 480
BciVI GTATCCnnnnn_n'
Cuts at: 3225 5415 6178 7519 3225
Size: 2190 763 1341 3225
Fragments arranged by size:
3225 2190 1341 763
BclI T'GATC_A
Cuts at: 1035 1623 2958 5037 1035
Size: 588 1335 2079 3517
Fragments arranged by size:
3517 2079 1335 588
BfrBI ATG'CAT
Cuts at: 3807 3807
Size: 7519
BglII A'GATC_T
Cuts at: 1458 3645 3789 5032 7071 1458
Size: 2187 144 1243 2039 1906
Fragments arranged by size:
2187 2039 1906 1243 144
BlpI GC'TnA_GC
Cuts at: 3284 4629 3284
Size: 1345 6174
Bme1580I G_kGCm'C
Cuts at: 5142 5235 6293 5142
Size: 93 1058 6368
Fragments arranged by size:
6368 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2588 4274 4729 4810 5145 7291 2588
Size: 1686 455 81 335 2146 2816
Fragments arranged by size:
2816 2146 1686 455 335 81
BmtI G_CTAG'C
Cuts at: 1138 1739 1138
Size: 601 6918
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1778 1975 5884 1778
Size: 197 3909 3413
Fragments arranged by size:
3909 3413 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2177 2687 3990 2177
Size: 510 1303 5706
Fragments arranged by size:
5706 1303 510
Bpu10I CC'TnA_GC
Cuts at: 2267 3006 3486 2267
Size: 739 480 6300
Fragments arranged by size:
6300 739 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1333 1816 1944 3905 4143 4693 5572 6066
Size: 275 483 128 1961 238 550 879 494
Cuts at: 6066 6364 1058
Size: 298 2213
Fragments arranged by size:
2213 1961 879 550 494 483 298 275
238 128
BsaI GGTCTCn'nnnn_
Cuts at: 1004 7223 1004
Size: 6219 1300
BsaAI yAC'GTr
Cuts at: 2557 4509 4870 2557
Size: 1952 361 5206
Fragments arranged by size:
5206 1952 361
BsaBI GATnn'nnATC
Cuts at: 6 404 1415 5031 7070 6
Size: 398 1011 3616 2039 455
Fragments arranged by size:
3616 2039 1011 455 398
BsaHI Gr'CG_yC
Cuts at: 1329 4948 5196 5900 7396 1329
Size: 3619 248 704 1496 1452
Fragments arranged by size:
3619 1496 1452 704 248
BsaWI w'CCGG_w
Cuts at: 345 3069 3572 4706 5227 5907 6181 6328 6739
Size: 2724 503 1134 521 680 274 147 411
Cuts at: 6739 345
Size: 1125
Fragments arranged by size:
2724 1134 1125 680 521 503 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2089 2119 2219 2249 2727 2757 2089
Size: 30 100 30 478 30 6851
Fragments arranged by size:
6851 478 100 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1142 2110 2315 1142
Size: 968 205 6346
Fragments arranged by size:
6346 968 205
BseYI C'CCAG_C
Cuts at: 1083 1276 1769 2362 4942 5700 6279 6775 1083
Size: 193 493 593 2580 758 579 496 1827
Fragments arranged by size:
2580 1827 758 593 579 496 493 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 780 968 1637 595
Size: 185 188 669 6477
Fragments arranged by size:
6477 669 188 185
BsiEI CG_ry'CG
Cuts at: 104 3900 5059 5105 6315 104
Size: 3796 1159 46 1210 1308
Fragments arranged by size:
3796 1308 1210 1159 46
BsiHKAI G_wGCw'C
Cuts at: 221 3150 4531 5309 5499 6293 221
Size: 2929 1381 778 190 794 1447
Fragments arranged by size:
2929 1447 1381 794 778 190
BsmI GAATG_Cn'
Cuts at: 4428 4428
Size: 7519
BsmBI CGTCTCn'nnnn_
Cuts at: 1749 1749
Size: 7519
Bsp1286I G_dGCh'C
Cuts at: 221 1284 1479 3150 4383 4531 5142 5235 5309
Size: 1063 195 1671 1233 148 611 93 74
Cuts at: 5309 5499 5561 6293 221
Size: 190 62 732 1447
Fragments arranged by size:
1671 1447 1233 1063 732 611 195 190
148 93 74 62
BspEI T'CCGG_A
Cuts at: 345 6739 345
Size: 6394 1125
BspHI T'CATG_A
Cuts at: 903 6695 903
Size: 5792 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4567 4781 5083 5464 4567
Size: 214 302 381 6622
Fragments arranged by size:
6622 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5809 5863 7349 7514 50
Size: 5759 54 1486 165 55
Fragments arranged by size:
5759 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3551 3656 5429 3551
Size: 105 1773 5641
Fragments arranged by size:
5641 1773 105
BsrFI r'CCGG_y
Cuts at: 5515 5696 5907 5515
Size: 181 211 7127
Fragments arranged by size:
7127 211 181
BsrGI T'GTAC_A
Cuts at: 3021 3021
Size: 7519
BssSI C'ACGA_G
Cuts at: 182 5788 6148 182
Size: 5606 360 1553
Fragments arranged by size:
5606 1553 360
BstAPI GCAn_nnn'nTGC
Cuts at: 562 955 3202 4225 4894 562
Size: 393 2247 1023 669 3187
Fragments arranged by size:
3187 2247 1023 669 393
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7519
BstXI CCAn_nnnn'nTGG
Cuts at: 745 2826 745
Size: 2081 5438
BstYI r'GATC_y
Cuts at: 1 1458 2478 3645 3789 4542 5009 5032 5367
Size: 1457 1020 1167 144 753 467 23 335
Cuts at: 5367 5613 6627 6713 6725 7071 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1457 1167 1020 1014 753 467 449 346
335 246 144 86 23 12
BstZ17I GTA'TAC
Cuts at: 1890 4450 1890
Size: 2560 4959
BtgI C'CryG_G
Cuts at: 617 639 617
Size: 22 7497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5467 5648 5811 5467
Size: 181 163 7175
Fragments arranged by size:
7175 181 163
BtsI GCAGTG_nn'
Cuts at: 162 3713 3737 4760 162
Size: 3551 24 1023 2921
Fragments arranged by size:
3551 2921 1023 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1950 1985 1950
Size: 35 7484
DraI TTT'AAA
Cuts at: 2175 4083 4516 2175
Size: 1908 433 5178
Fragments arranged by size:
5178 1908 433
DraIII CAC_nnn'GTG
Cuts at: 2245 2245
Size: 7519
DrdI GACnn_nn'nnGTC
Cuts at: 3469 5223 6083 3469
Size: 1754 860 4905
Fragments arranged by size:
4905 1754 860
EaeI y'GGCC_r
Cuts at: 4362 5102 5276 5667 5694 7041 4362
Size: 740 174 391 27 1347 4840
Fragments arranged by size:
4840 1347 740 391 174 27
EagI C'GGCC_G
Cuts at: 5102 5102
Size: 7519
EarI CTCTTCn'nnn_
Cuts at: 198 1172 1664 1769 3366 3617 3773 4388 4475
Size: 974 492 105 1597 251 156 615 87
Cuts at: 4475 5540 5750 198
Size: 1065 210 1967
Fragments arranged by size:
1967 1597 1065 974 615 492 251 210
156 105 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3975 5725 6037 6183 7329 3975
Size: 1750 312 146 1146 4165
Fragments arranged by size:
4165 1750 1146 312 146
EcoICRI GAG'CTC
Cuts at: 219 4529 219
Size: 4310 3209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 466 485 590 611 746 1846 2177 2687
Size: 2 19 105 21 135 1100 331 510
Cuts at: 2687 2759 3149 3230 3353 3488 3990 5342 5774
Size: 72 390 81 123 135 502 1352 432
Cuts at: 5774 6523 6791 464
Size: 749 268 1192
Fragments arranged by size:
1352 1192 1100 749 510 502 432 390
331 268 135 135 123 105 81 72
21 19 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3245 3363 3972 3245
Size: 118 609 6792
Fragments arranged by size:
6792 609 118
EcoO109I rG'GnC_Cy
Cuts at: 438 4656 438
Size: 4218 3301
EcoRI G'AATT_C
Cuts at: 865 4521 865
Size: 3656 3863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 469 832 2489 5536 469
Size: 363 1657 3047 2452
Fragments arranged by size:
3047 2452 1657 363
FspI TGC'GCA
Cuts at: 3142 5298 3142
Size: 2156 5363
HaeII r_GCGC'y
Cuts at: 1044 1293 3536 4951 5199 6223 7202 1044
Size: 249 2243 1415 248 1024 979 1361
Fragments arranged by size:
2243 1415 1361 1024 979 249 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 775 807 2899 2931 2935 2967 3181 3213 3474
Size: 32 2092 32 4 32 214 32 261
Cuts at: 3474 3506 3600 3632 5359 5389 5391 5421 775
Size: 32 94 32 1727 30 2 30 2873
Fragments arranged by size:
2873 2092 1727 261 214 94 32 32
32 32 32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 2687 4556 2687
Size: 1869 5650
HindIII A'AGCT_T
Cuts at: 3161 3161
Size: 7519
KasI G'GCGC_C
Cuts at: 4947 5195 4947
Size: 248 7271
KpnI G_GTAC'C
Cuts at: 4537 4537
Size: 7519
MluI A'CGCG_T
Cuts at: 6952 6952
Size: 7519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 180 6190 6374 180
Size: 6010 184 1325
Fragments arranged by size:
6010 1325 184
MscI TGG'CCA
Cuts at: 4364 5278 4364
Size: 914 6605
MslI CAynn'nnrTG
Cuts at: 756 1247 1702 2824 5506 5633 756
Size: 491 455 1122 2682 127 2642
Fragments arranged by size:
2682 2642 1122 491 455 127
MspA1I CmG'CkG
Cuts at: 799 3010 4060 4212 4411 4628 4942 5302 6317
Size: 2211 1050 152 199 217 314 360 1015
Cuts at: 6317 6562 799
Size: 245 1756
Fragments arranged by size:
2211 1756 1050 1015 360 314 245 217
199 152
NaeI GCC'GGC
Cuts at: 5698 5698
Size: 7519
NarI GG'CG_CC
Cuts at: 4948 5196 4948
Size: 248 7271
NcoI C'CATG_G
Cuts at: 617 617
Size: 7519
NgoMIV G'CCGG_C
Cuts at: 5696 5696
Size: 7519
NheI G'CTAG_C
Cuts at: 1134 1735 1134
Size: 601 6918
NruI TCG'CGA
Cuts at: 5880 5880
Size: 7519
NsiI A_TGCA'T
Cuts at: 3809 3809
Size: 7519
NspI r_CATG'y
Cuts at: 958 3022 4570 5070 5979 958
Size: 2064 1548 500 909 2498
Fragments arranged by size:
2498 2064 1548 909 500
PasI CC'CwG_GG
Cuts at: 991 991
Size: 7519
PciI A'CATG_T
Cuts at: 5975 5975
Size: 7519
PflMI CCAn_nnn'nTGG
Cuts at: 655 4360 655
Size: 3705 3814
PfoI T'CCnGG_A
Cuts at: 1065 2010 1065
Size: 945 6574
PmeI GTTT'AAAC
Cuts at: 4516 4516
Size: 7519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6658 6690 6658
Size: 32 7487
PpuMI rG'GwC_Cy
Cuts at: 438 438
Size: 7519
PsiI TTA'TAA
Cuts at: 1427 2600 1427
Size: 1173 6346
PstI C_TGCA'G
Cuts at: 798 1494 4564 5249 798
Size: 696 3070 685 3068
Fragments arranged by size:
3070 3068 696 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7519
PvuII CAG'CTG
Cuts at: 799 3010 4060 4212 4411 4942 5302 799
Size: 2211 1050 152 199 531 360 3016
Fragments arranged by size:
3016 2211 1050 531 360 199 152
RsrII CG'GwC_CG
Cuts at: 5712 5712
Size: 7519
SacI G_AGCT'C
Cuts at: 221 4531 221
Size: 4310 3209
SalI G'TCGA_C
Cuts at: 4554 4554
Size: 7519
SanDI GG'GwC_CC
Cuts at: 438 438
Size: 7519
SapI GCTCTTCn'nnn_
Cuts at: 4388 5540 5750 4388
Size: 1152 210 6157
Fragments arranged by size:
6157 1152 210
SbfI CC_TGCA'GG
Cuts at: 4564 4564
Size: 7519
ScaI AGT'ACT
Cuts at: 815 1713 7036 815
Size: 898 5323 1298
Fragments arranged by size:
5323 1298 898
SfcI C'TryA_G
Cuts at: 33 416 794 828 1293 1354 1490 2351 4046
Size: 383 378 34 465 61 136 861 1695
Cuts at: 4046 4560 5245 6240 6431 33
Size: 514 685 995 191 1121
Fragments arranged by size:
1695 1121 995 861 685 514 465 383
378 191 136 61 34
SfoI GGC'GCC
Cuts at: 4949 5197 4949
Size: 248 7271
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7519
SmaI CCC'GGG
Cuts at: 4343 4539 4343
Size: 196 7323
SmlI C'TyrA_G
Cuts at: 454 1073 1312 1323 1795 1923 3920 4158 4672
Size: 619 239 11 472 128 1997 238 514
Cuts at: 4672 5587 6081 6343 454
Size: 915 494 262 1630
Fragments arranged by size:
1997 1630 915 619 514 494 472 262
239 238 128 11
SnaBI TAC'GTA
Cuts at: 4509 4509
Size: 7519
SpeI A'CTAG_T
Cuts at: 67 3402 3553 67
Size: 3335 151 4033
Fragments arranged by size:
4033 3335 151
SphI G_CATG'C
Cuts at: 958 4570 5070 958
Size: 3612 500 3407
Fragments arranged by size:
3612 3407 500
SspI AAT'ATT
Cuts at: 4076 4076
Size: 7519
StuI AGG'CCT
Cuts at: 1434 1434
Size: 7519
StyI C'CwwG_G
Cuts at: 617 1730 3109 3467 4651 5005 617
Size: 1113 1379 358 1184 354 3131
Fragments arranged by size:
3131 1379 1184 1113 358 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5100 5233 5692 5903 5100
Size: 133 459 211 6716
Fragments arranged by size:
6716 459 211 133
TatI w'GTAC_w
Cuts at: 420 813 1214 1711 2647 3021 4497 7034 420
Size: 393 401 497 936 374 1476 2537 905
Fragments arranged by size:
2537 1476 936 905 497 401 393 374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2089 2121 2219 2251 2727 2759 7036 7068 2089
Size: 32 98 32 476 32 4277 32 2540
Fragments arranged by size:
4277 2540 476 98 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5314 5314
Size: 7519
XbaI T'CTAG_A
Cuts at: 2541 3346 4548 2541
Size: 805 1202 5512
Fragments arranged by size:
5512 1202 805
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5012 5012
Size: 7519
XmaI C'CCGG_G
Cuts at: 4341 4537 4341
Size: 196 7323
XmnI GAAnn'nnTTC
Cuts at: 2387 2387
Size: 7519
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AlfI AloI ApaI AscI AvrII
BaeI BcgI BglI BmgBI BsiWI BssHII BstEII Bsu36I
ClaI EcoRV FseI FspAI HpaI MfeI NdeI NotI
PacI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI SwaI XhoI ZraI