Restriction map for
pF1KSDA0457
(Circular) (Six-base) MAPSORT of: pF1KSDA0457.seq Check: 9486 from: 1 to: 5635
pF1KSDA0457 5635 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 222 222
Size: 5635
AccI GT'mk_AC
Cuts at: 2671 2671
Size: 5635
Acc65I G'GTAC_C
Cuts at: 2649 2649
Size: 5635
AclI AA'CG_TT
Cuts at: 5475 5475
Size: 5635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 906 1180 1189 1486 3458 3890 4639 4907 906
Size: 274 9 297 1972 432 749 268 1634
Fragments arranged by size:
1972 1634 749 432 297 274 268 9
AflII C'TTAA_G
Cuts at: 1182 2149 1182
Size: 967 4668
AflIII A'CryG_T
Cuts at: 1472 2985 4091 5068 1472
Size: 1513 1106 977 2039
Fragments arranged by size:
2039 1513 1106 977
AgeI A'CCGG_T
Cuts at: 4023 4023
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 523 581 825 1708 2604 523
Size: 58 244 883 896 3554
Fragments arranged by size:
3554 896 883 244 58
AlwNI CAG_nnn'CTG
Cuts at: 280 2938 4507 280
Size: 2658 1569 1408
Fragments arranged by size:
2658 1569 1408
ApaLI G'TGCA_C
Cuts at: 4405 4405
Size: 5635
ApoI r'AATT_y
Cuts at: 1831 2637 4988 5244 1831
Size: 806 2351 256 2222
Fragments arranged by size:
2351 2222 806 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5635
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5635
AvaI C'yCGr_G
Cuts at: 393 2653 393
Size: 2260 3375
BamHI G'GATC_C
Cuts at: 1041 2658 1041
Size: 1617 4018
BanI G'GyrC_C
Cuts at: 148 499 2649 3063 3311 3346 148
Size: 351 2150 414 248 35 2437
Fragments arranged by size:
2437 2150 414 351 248 35
BanII G_rGCy'C
Cuts at: 418 850 2504 2647 3677 418
Size: 432 1654 143 1030 2376
Fragments arranged by size:
2376 1654 1030 432 143
BbeI G_GCGC'C
Cuts at: 152 3067 3315 152
Size: 2915 248 2472
Fragments arranged by size:
2915 2472 248
BbsI GAAGACnn'nnnn_
Cuts at: 1274 2359 2414 1274
Size: 1085 55 4495
Fragments arranged by size:
4495 1085 55
BbvCI CC'TCA_GC
Cuts at: 1710 1999 1710
Size: 289 5346
BciVI GTATCCnnnnn_n'
Cuts at: 3531 4294 5635 3531
Size: 763 1341 3531
Fragments arranged by size:
3531 1341 763
BclI T'GATC_A
Cuts at: 3153 3153
Size: 5635
BfrBI ATG'CAT
Cuts at: 999 1905 999
Size: 906 4729
BglI GCCn_nnn'nGGC
Cuts at: 516 516
Size: 5635
BglII A'GATC_T
Cuts at: 2475 3148 5187 2475
Size: 673 2039 2923
Fragments arranged by size:
2923 2039 673
BlpI GC'TnA_GC
Cuts at: 520 2745 520
Size: 2225 3410
Bme1580I G_kGCm'C
Cuts at: 313 3258 3351 4409 313
Size: 2945 93 1058 1539
Fragments arranged by size:
2945 1539 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2845 2926 3261 5407 2845
Size: 81 335 2146 3073
Fragments arranged by size:
3073 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 963 1014 1600 1747 2008 2230 2488 2530 963
Size: 51 586 147 261 222 258 42 4068
Fragments arranged by size:
4068 586 261 258 222 147 51 42
Bpu10I CC'TnA_GC
Cuts at: 1083 1114 1457 1710 1999 1083
Size: 31 343 253 289 4719
Fragments arranged by size:
4719 343 289 253 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 732 2809 3688 4182 4480 732
Size: 2077 879 494 298 1887
Fragments arranged by size:
2077 1887 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 580 948 5339 580
Size: 368 4391 876
Fragments arranged by size:
4391 876 368
BsaAI yAC'GTr
Cuts at: 1473 2625 2986 1473
Size: 1152 361 4122
Fragments arranged by size:
4122 1152 361
BsaBI GATnn'nnATC
Cuts at: 6 2474 3147 5186 6
Size: 2468 673 2039 455
Fragments arranged by size:
2468 2039 673 455
BsaHI Gr'CG_yC
Cuts at: 149 596 3064 3312 4016 5512 149
Size: 447 2468 248 704 1496 272
Fragments arranged by size:
2468 1496 704 447 272 248
BsaWI w'CCGG_w
Cuts at: 320 410 1157 1743 2822 3343 4023 4297 4444
Size: 90 747 586 1079 521 680 274 147
Cuts at: 4444 4855 320
Size: 411 1100
Fragments arranged by size:
1100 1079 747 680 586 521 411 274
147 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1009 1039 1009
Size: 30 5605
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 361 1017 1612 1966 1987 2303 361
Size: 656 595 354 21 316 3693
Fragments arranged by size:
3693 656 595 354 316 21
BseYI C'CCAG_C
Cuts at: 153 300 547 602 2040 3058 3816 4395 4891
Size: 147 247 55 1438 1018 758 579 496
Cuts at: 4891 153
Size: 897
Fragments arranged by size:
1438 1018 897 758 579 496 247 147
55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 1279 2184 452
Size: 827 905 3903
Fragments arranged by size:
3903 905 827
BsiEI CG_ry'CG
Cuts at: 104 3175 3221 4431 104
Size: 3071 46 1210 1308
Fragments arranged by size:
3071 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2090 2504 2647 3425 3615 4409 2090
Size: 414 143 778 190 794 3316
Fragments arranged by size:
3316 794 778 414 190 143
BsmI GAATG_Cn'
Cuts at: 999 999
Size: 5635
BsmBI CGTCTCn'nnnn_
Cuts at: 1240 1479 1240
Size: 239 5396
Bsp1286I G_dGCh'C
Cuts at: 313 418 850 2090 2504 2647 3258 3351 3425
Size: 105 432 1240 414 143 611 93 74
Cuts at: 3425 3615 3677 4409 313
Size: 190 62 732 1539
Fragments arranged by size:
1539 1240 732 611 432 414 190 143
105 93 74 62
BspEI T'CCGG_A
Cuts at: 410 4855 410
Size: 4445 1190
BspHI T'CATG_A
Cuts at: 2466 4811 2466
Size: 2345 3290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 222 2683 2897 3199 3580 222
Size: 2461 214 302 381 2277
Fragments arranged by size:
2461 2277 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3925 3979 5465 5630 50
Size: 3875 54 1486 165 55
Fragments arranged by size:
3875 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 837 3545 837
Size: 2708 2927
BsrFI r'CCGG_y
Cuts at: 163 1714 3631 3812 4023 163
Size: 1551 1917 181 211 1775
Fragments arranged by size:
1917 1775 1551 211 181
BssSI C'ACGA_G
Cuts at: 892 1482 3904 4264 892
Size: 590 2422 360 2263
Fragments arranged by size:
2422 2263 590 360
BstAPI GCAn_nnn'nTGC
Cuts at: 683 3010 683
Size: 2327 3308
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1342 1342
Size: 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 1038 1038
Size: 5635
BstYI r'GATC_y
Cuts at: 1 1041 2475 2658 3125 3148 3483 3729 4743
Size: 1040 1434 183 467 23 335 246 1014
Cuts at: 4743 4829 4841 5187 1
Size: 86 12 346 449
Fragments arranged by size:
1434 1040 1014 467 449 346 335 246
183 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 143 704 1846 143
Size: 561 1142 3932
Fragments arranged by size:
3932 1142 561
BtgI C'CryG_G
Cuts at: 572 1377 572
Size: 805 4830
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 635 918 3583 3764 3927 635
Size: 283 2665 181 163 2343
Fragments arranged by size:
2665 2343 283 181 163
BtsI GCAGTG_nn'
Cuts at: 311 1451 2179 2876 311
Size: 1140 728 697 3070
Fragments arranged by size:
3070 1140 728 697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1600 1635 1600
Size: 35 5600
DraI TTT'AAA
Cuts at: 2632 2632
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 3339 4199 3339
Size: 860 4775
EaeI y'GGCC_r
Cuts at: 526 3218 3392 3783 3810 5157 526
Size: 2692 174 391 27 1347 1004
Fragments arranged by size:
2692 1347 1004 391 174 27
EagI C'GGCC_G
Cuts at: 3218 3218
Size: 5635
EarI CTCTTCn'nnn_
Cuts at: 2070 3656 3866 2070
Size: 1586 210 3839
Fragments arranged by size:
3839 1586 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3841 4153 4299 5445 3841
Size: 312 146 1146 4031
Fragments arranged by size:
4031 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2502 2645 2502
Size: 143 5492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 906 963 1014 1180 1189 1486 1600 1747 2008
Size: 57 51 166 9 297 114 147 261
Cuts at: 2008 2230 2488 2530 3458 3890 4639 4907 906
Size: 222 258 42 928 432 749 268 1634
Fragments arranged by size:
1634 928 749 432 297 268 261 258
222 166 147 114 57 51 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1948 1948
Size: 5635
EcoO109I rG'GnC_Cy
Cuts at: 139 277 708 897 2772 139
Size: 138 431 189 1875 3002
Fragments arranged by size:
3002 1875 431 189 138
EcoRI G'AATT_C
Cuts at: 2637 2637
Size: 5635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 3652 2461
Size: 1191 4444
FspI TGC'GCA
Cuts at: 3414 3414
Size: 5635
HaeII r_GCGC'y
Cuts at: 152 3067 3315 4339 5318 152
Size: 2915 248 1024 979 469
Fragments arranged by size:
2915 1024 979 469 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 817 849 1009 1041 1081 1113 1162 1194 1945
Size: 32 160 32 40 32 49 32 751
Cuts at: 1945 1977 2213 2245 3475 3505 3507 3537 817
Size: 32 236 32 1230 30 2 30 2915
Fragments arranged by size:
2915 1230 751 236 160 49 40 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1860 2672 1860
Size: 812 4823
HindIII A'AGCT_T
Cuts at: 750 2213 750
Size: 1463 4172
HpaI GTT'AAC
Cuts at: 1860 1860
Size: 5635
KasI G'GCGC_C
Cuts at: 148 3063 3311 148
Size: 2915 248 2472
Fragments arranged by size:
2915 2472 248
KpnI G_GTAC'C
Cuts at: 2653 2653
Size: 5635
MluI A'CGCG_T
Cuts at: 5068 5068
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1424 4306 4490 1424
Size: 2882 184 2569
Fragments arranged by size:
2882 2569 184
MscI TGG'CCA
Cuts at: 3394 3394
Size: 5635
MslI CAynn'nnrTG
Cuts at: 2465 3622 3749 2465
Size: 1157 127 4351
Fragments arranged by size:
4351 1157 127
MspA1I CmG'CkG
Cuts at: 602 683 1079 1400 1581 1656 1671 2168 2599
Size: 81 396 321 181 75 15 497 431
Cuts at: 2599 2744 3058 3418 4433 4678 602
Size: 145 314 360 1015 245 1559
Fragments arranged by size:
1559 1015 497 431 396 360 321 314
245 181 145 81 75 15
NaeI GCC'GGC
Cuts at: 165 3814 165
Size: 3649 1986
NarI GG'CG_CC
Cuts at: 149 3064 3312 149
Size: 2915 248 2472
Fragments arranged by size:
2915 2472 248
NcoI C'CATG_G
Cuts at: 572 572
Size: 5635
NgoMIV G'CCGG_C
Cuts at: 163 3812 163
Size: 3649 1986
NruI TCG'CGA
Cuts at: 3996 3996
Size: 5635
NsiI A_TGCA'T
Cuts at: 1001 1907 1001
Size: 906 4729
NspI r_CATG'y
Cuts at: 999 1909 2686 3186 4095 999
Size: 910 777 500 909 2539
Fragments arranged by size:
2539 910 909 777 500
PasI CC'CwG_GG
Cuts at: 538 1662 538
Size: 1124 4511
PciI A'CATG_T
Cuts at: 4091 4091
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 858 2185 858
Size: 1327 4308
PfoI T'CCnGG_A
Cuts at: 1763 2023 2246 1763
Size: 260 223 5152
Fragments arranged by size:
5152 260 223
PmeI GTTT'AAAC
Cuts at: 2632 2632
Size: 5635
PmlI CAC'GTG
Cuts at: 1473 1473
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4774 4806 4774
Size: 32 5603
PpuMI rG'GwC_Cy
Cuts at: 139 708 897 139
Size: 569 189 4877
Fragments arranged by size:
4877 569 189
PstI C_TGCA'G
Cuts at: 219 619 688 951 1676 1840 2388 2528 2543
Size: 400 69 263 725 164 548 140 15
Cuts at: 2543 2583 2680 3365 219
Size: 40 97 685 2489
Fragments arranged by size:
2489 725 685 548 400 263 164 140
97 69 40 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5635
PvuII CAG'CTG
Cuts at: 602 683 1581 1656 1671 2168 2599 3058 3418
Size: 81 898 75 15 497 431 459 360
Cuts at: 3418 602
Size: 2819
Fragments arranged by size:
2819 898 497 459 431 360 81 75
15
RsrII CG'GwC_CG
Cuts at: 3828 3828
Size: 5635
SacI G_AGCT'C
Cuts at: 2504 2647 2504
Size: 143 5492
SalI G'TCGA_C
Cuts at: 2670 2670
Size: 5635
SapI GCTCTTCn'nnn_
Cuts at: 3656 3866 3656
Size: 210 5425
SbfI CC_TGCA'GG
Cuts at: 2680 2680
Size: 5635
ScaI AGT'ACT
Cuts at: 5152 5152
Size: 5635
SfcI C'TryA_G
Cuts at: 33 215 615 684 947 1519 1672 1836 2239
Size: 182 400 69 263 572 153 164 403
Cuts at: 2239 2270 2384 2524 2539 2579 2676 3361 4356
Size: 31 114 140 15 40 97 685 995
Cuts at: 4356 4547 33
Size: 191 1121
Fragments arranged by size:
1121 995 685 572 403 400 263 191
182 164 153 140 114 97 69 40
31 15
SfoI GGC'GCC
Cuts at: 150 3065 3313 150
Size: 2915 248 2472
Fragments arranged by size:
2915 2472 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 163 163
Size: 5635
SmaI CCC'GGG
Cuts at: 2655 2655
Size: 5635
SmlI C'TyrA_G
Cuts at: 393 747 1182 2149 2788 3703 4197 4459 393
Size: 354 435 967 639 915 494 262 1569
Fragments arranged by size:
1569 967 915 639 494 435 354 262
SnaBI TAC'GTA
Cuts at: 2625 2625
Size: 5635
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5635
SphI G_CATG'C
Cuts at: 1909 2686 3186 1909
Size: 777 500 4358
Fragments arranged by size:
4358 777 500
StuI AGG'CCT
Cuts at: 401 1690 401
Size: 1289 4346
StyI C'CwwG_G
Cuts at: 572 2767 3121 572
Size: 2195 354 3086
Fragments arranged by size:
3086 2195 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 539 1426 3216 3349 3808 4019 539
Size: 887 1790 133 459 211 2155
Fragments arranged by size:
2155 1790 887 459 211 133
TatI w'GTAC_w
Cuts at: 2135 2429 5150 2135
Size: 294 2721 2620
Fragments arranged by size:
2721 2620 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 349 381 5152 5184 349
Size: 32 4771 32 800
Fragments arranged by size:
4771 800 32 32
Tth111I GACn'n_nGTC
Cuts at: 3430 3430
Size: 5635
XbaI T'CTAG_A
Cuts at: 2664 2664
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3128 3128
Size: 5635
XhoI C'TCGA_G
Cuts at: 393 393
Size: 5635
XmaI C'CCGG_G
Cuts at: 2653 2653
Size: 5635
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BcgI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I
ClaI DraIII EcoRV FseI FspAI MfeI NdeI NheI
NotI PacI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SrfI SspI SwaI XmnI ZraI