Restriction map for
pF1KSDA0448
(Circular) (Six-base) MAPSORT of: pF1KSDA0448.seq Check: 3746 from: 1 to: 4207
pF1KSDA0448 4207 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 815 815
Size: 4207
AccI GT'mk_AC
Cuts at: 1243 1243
Size: 4207
Acc65I G'GTAC_C
Cuts at: 1221 1221
Size: 4207
AclI AA'CG_TT
Cuts at: 4047 4047
Size: 4207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 2030 2462 3211 3479 908
Size: 1122 432 749 268 1636
Fragments arranged by size:
1636 1122 749 432 268
AflIII A'CryG_T
Cuts at: 549 1557 2663 3640 549
Size: 1008 1106 977 1116
Fragments arranged by size:
1116 1106 1008 977
AgeI A'CCGG_T
Cuts at: 2595 2595
Size: 4207
AhdI GACnn_n'nnGTC
Cuts at: 271 271
Size: 4207
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1054 1086 1054
Size: 32 4175
AlwNI CAG_nnn'CTG
Cuts at: 168 225 1510 3079 168
Size: 57 1285 1569 1296
Fragments arranged by size:
1569 1296 1285 57
ApaLI G'TGCA_C
Cuts at: 2977 2977
Size: 4207
ApoI r'AATT_y
Cuts at: 574 1078 1209 3560 3816 574
Size: 504 131 2351 256 965
Fragments arranged by size:
2351 965 504 256 131
AseI AT'TA_AT
Cuts at: 20 605 860 20
Size: 585 255 3367
Fragments arranged by size:
3367 585 255
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4207
AvaI C'yCGr_G
Cuts at: 1225 1225
Size: 4207
BamHI G'GATC_C
Cuts at: 618 1230 618
Size: 612 3595
BanI G'GyrC_C
Cuts at: 1221 1635 1883 1918 1221
Size: 414 248 35 3510
Fragments arranged by size:
3510 414 248 35
BanII G_rGCy'C
Cuts at: 134 1113 1219 2249 134
Size: 979 106 1030 2092
Fragments arranged by size:
2092 1030 979 106
BbeI G_GCGC'C
Cuts at: 1639 1887 1639
Size: 248 3959
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2866 4207 2103
Size: 763 1341 2103
Fragments arranged by size:
2103 1341 763
BclI T'GATC_A
Cuts at: 350 1725 350
Size: 1375 2832
BglII A'GATC_T
Cuts at: 1720 3759 1720
Size: 2039 2168
BlpI GC'TnA_GC
Cuts at: 1317 1317
Size: 4207
Bme1580I G_kGCm'C
Cuts at: 1830 1923 2981 1830
Size: 93 1058 3056
Fragments arranged by size:
3056 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1417 1498 1833 3979 1417
Size: 81 335 2146 1645
Fragments arranged by size:
2146 1645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 2572 255
Size: 2317 1890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 740 247
Size: 493 3714
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 2260 2754 3052 1381
Size: 879 494 298 2536
Fragments arranged by size:
2536 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1130 3911 1130
Size: 2781 1426
BsaAI yAC'GTr
Cuts at: 1197 1558 1197
Size: 361 3846
BsaBI GATnn'nnATC
Cuts at: 6 1719 3758 6
Size: 1713 2039 455
Fragments arranged by size:
2039 1713 455
BsaHI Gr'CG_yC
Cuts at: 1636 1884 2588 4084 1636
Size: 248 704 1496 1759
Fragments arranged by size:
1759 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1394 1915 2595 2869 3016 3427 1394
Size: 521 680 274 147 411 2174
Fragments arranged by size:
2174 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1054 1084 1054
Size: 30 4177
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 124 244 124
Size: 120 4087
BseYI C'CCAG_C
Cuts at: 1630 2388 2967 3463 1630
Size: 758 579 496 2374
Fragments arranged by size:
2374 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 1747 1793 3003 104
Size: 1643 46 1210 1308
Fragments arranged by size:
1643 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 756 1219 1997 2187 2981 756
Size: 463 778 190 794 1982
Fragments arranged by size:
1982 794 778 463 190
BsmI GAATG_Cn'
Cuts at: 811 811
Size: 4207
BsmBI CGTCTCn'nnnn_
Cuts at: 684 684
Size: 4207
Bsp1286I G_dGCh'C
Cuts at: 134 756 1113 1219 1830 1923 1997 2187 2249
Size: 622 357 106 611 93 74 190 62
Cuts at: 2249 2981 134
Size: 732 1360
Fragments arranged by size:
1360 732 622 611 357 190 106 93
74 62
BspEI T'CCGG_A
Cuts at: 3427 3427
Size: 4207
BspHI T'CATG_A
Cuts at: 3383 3383
Size: 4207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 815 1255 1469 1771 2152 815
Size: 440 214 302 381 2870
Fragments arranged by size:
2870 440 381 302 214
BsrBI CCG'CTC
Cuts at: 50 239 2497 2551 4037 4202 50
Size: 189 2258 54 1486 165 55
Fragments arranged by size:
2258 1486 189 165 55 54
BsrDI GCAATG_nn'
Cuts at: 475 925 1025 2117 475
Size: 450 100 1092 2565
Fragments arranged by size:
2565 1092 450 100
BsrFI r'CCGG_y
Cuts at: 2203 2384 2595 2203
Size: 181 211 3815
Fragments arranged by size:
3815 211 181
BssSI C'ACGA_G
Cuts at: 2476 2836 2476
Size: 360 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4207
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4207
BstYI r'GATC_y
Cuts at: 1 215 618 1230 1697 1720 2055 2301 3315
Size: 214 403 612 467 23 335 246 1014
Cuts at: 3315 3401 3413 3759 1
Size: 86 12 346 449
Fragments arranged by size:
1014 612 467 449 403 346 335 246
214 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 136 136
Size: 4207
BtgI C'CryG_G
Cuts at: 125 125
Size: 4207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 208 2155 2336 2499 208
Size: 1947 181 163 1916
Fragments arranged by size:
1947 1916 181 163
BtsI GCAGTG_nn'
Cuts at: 1448 1448
Size: 4207
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4207
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2771 1911
Size: 860 3347
EaeI y'GGCC_r
Cuts at: 792 1790 1964 2355 2382 3729 792
Size: 998 174 391 27 1347 1270
Fragments arranged by size:
1347 1270 998 391 174 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4207
EarI CTCTTCn'nnn_
Cuts at: 205 332 2228 2438 205
Size: 127 1896 210 1974
Fragments arranged by size:
1974 1896 210 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2413 2725 2871 4017 2413
Size: 312 146 1146 2603
Fragments arranged by size:
2603 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1217 1217
Size: 4207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 740 908 2030 2462 3211 3479 247
Size: 493 168 1122 432 749 268 975
Fragments arranged by size:
1122 975 749 493 432 268 168
EcoO109I rG'GnC_Cy
Cuts at: 1344 1344
Size: 4207
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4207
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4207
HaeII r_GCGC'y
Cuts at: 1639 1887 2911 3890 1639
Size: 248 1024 979 1956
Fragments arranged by size:
1956 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 257 289 2047 2077 2079 2109 257
Size: 32 1758 30 2 30 2355
Fragments arranged by size:
2355 1758 32 30 30 2
HincII GTy'rAC
Cuts at: 1244 1244
Size: 4207
KasI G'GCGC_C
Cuts at: 1635 1883 1635
Size: 248 3959
KpnI G_GTAC'C
Cuts at: 1225 1225
Size: 4207
MluI A'CGCG_T
Cuts at: 3640 3640
Size: 4207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2878 3062 2878
Size: 184 4023
MscI TGG'CCA
Cuts at: 794 1966 794
Size: 1172 3035
MslI CAynn'nnrTG
Cuts at: 2194 2321 2194
Size: 127 4080
MspA1I CmG'CkG
Cuts at: 165 1316 1630 1990 3005 3250 165
Size: 1151 314 360 1015 245 1122
Fragments arranged by size:
1151 1122 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4207
NarI GG'CG_CC
Cuts at: 1636 1884 1636
Size: 248 3959
NcoI C'CATG_G
Cuts at: 125 125
Size: 4207
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4207
NruI TCG'CGA
Cuts at: 2568 2568
Size: 4207
NspI r_CATG'y
Cuts at: 1258 1758 2667 1258
Size: 500 909 2798
Fragments arranged by size:
2798 909 500
PciI A'CATG_T
Cuts at: 2663 2663
Size: 4207
PflMI CCAn_nnn'nTGG
Cuts at: 225 225
Size: 4207
PfoI T'CCnGG_A
Cuts at: 514 514
Size: 4207
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1054 1086 3346 3378 1054
Size: 32 2260 32 1883
Fragments arranged by size:
2260 1883 32 32
PsiI TTA'TAA
Cuts at: 359 359
Size: 4207
PstI C_TGCA'G
Cuts at: 1252 1937 1252
Size: 685 3522
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4207
PvuII CAG'CTG
Cuts at: 165 1630 1990 165
Size: 1465 360 2382
Fragments arranged by size:
2382 1465 360
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4207
SacI G_AGCT'C
Cuts at: 1219 1219
Size: 4207
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4207
SapI GCTCTTCn'nnn_
Cuts at: 205 2228 2438 205
Size: 2023 210 1974
Fragments arranged by size:
2023 1974 210
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4207
ScaI AGT'ACT
Cuts at: 3724 3724
Size: 4207
SfcI C'TryA_G
Cuts at: 33 1036 1248 1933 2928 3119 33
Size: 1003 212 685 995 191 1121
Fragments arranged by size:
1121 1003 995 685 212 191
SfoI GGC'GCC
Cuts at: 1637 1885 1637
Size: 248 3959
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4207
SmaI CCC'GGG
Cuts at: 1227 1227
Size: 4207
SmlI C'TyrA_G
Cuts at: 1360 2275 2769 3031 1360
Size: 915 494 262 2536
Fragments arranged by size:
2536 915 494 262
SnaBI TAC'GTA
Cuts at: 1197 1197
Size: 4207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4207
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3707
SspI AAT'ATT
Cuts at: 868 868
Size: 4207
StyI C'CwwG_G
Cuts at: 125 1339 1693 125
Size: 1214 354 2639
Fragments arranged by size:
2639 1214 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1788 1921 2380 2591 1788
Size: 133 459 211 3404
Fragments arranged by size:
3404 459 211 133
TatI w'GTAC_w
Cuts at: 3722 3722
Size: 4207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3724 3756 3724
Size: 32 4175
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4207
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 764 1700 764
Size: 936 3271
XmaI C'CCGG_G
Cuts at: 1225 1225
Size: 4207
XmnI GAAnn'nnTTC
Cuts at: 869 1069 869
Size: 200 4007
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI