Restriction map for
pF1KSDA0427
(Circular) (Six-base) MAPSORT of: pF1KSDA0427.seq Check: 7162 from: 1 to: 4933
pF1KSDA0427 4933 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1648 1648
Size: 4933
AccI GT'mk_AC
Cuts at: 1969 1969
Size: 4933
Acc65I G'GTAC_C
Cuts at: 1947 1947
Size: 4933
AclI AA'CG_TT
Cuts at: 4773 4773
Size: 4933
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 2756 3188 3937 4205 432
Size: 2324 432 749 268 1160
Fragments arranged by size:
2324 1160 749 432 268
AfeI AGC'GCT
Cuts at: 202 1543 202
Size: 1341 3592
AflIII A'CryG_T
Cuts at: 2283 3389 4366 2283
Size: 1106 977 2850
Fragments arranged by size:
2850 1106 977
AgeI A'CCGG_T
Cuts at: 3321 3321
Size: 4933
AlwNI CAG_nnn'CTG
Cuts at: 243 341 534 1911 2236 3805 243
Size: 98 193 1377 325 1569 1371
Fragments arranged by size:
1569 1377 1371 325 193 98
ApaLI G'TGCA_C
Cuts at: 3703 3703
Size: 4933
ApoI r'AATT_y
Cuts at: 1935 4286 4542 1935
Size: 2351 256 2326
Fragments arranged by size:
2351 2326 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4933
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4933
AvaI C'yCGr_G
Cuts at: 353 1006 1117 1951 353
Size: 653 111 834 3335
Fragments arranged by size:
3335 834 653 111
BamHI G'GATC_C
Cuts at: 1956 1956
Size: 4933
BanI G'GyrC_C
Cuts at: 454 875 1582 1947 2361 2609 2644 454
Size: 421 707 365 414 248 35 2743
Fragments arranged by size:
2743 707 421 414 365 248 35
BanII G_rGCy'C
Cuts at: 366 817 1610 1655 1763 1844 1945 2975 366
Size: 451 793 45 108 81 101 1030 2324
Fragments arranged by size:
2324 1030 793 451 108 101 81 45
BbeI G_GCGC'C
Cuts at: 2365 2613 2365
Size: 248 4685
BbsI GAAGACnn'nnnn_
Cuts at: 1151 1569 1151
Size: 418 4515
BciVI GTATCCnnnnn_n'
Cuts at: 2829 3592 4933 2829
Size: 763 1341 2829
Fragments arranged by size:
2829 1341 763
BclI T'GATC_A
Cuts at: 2451 2451
Size: 4933
BglI GCCn_nnn'nGGC
Cuts at: 1042 1042
Size: 4933
BglII A'GATC_T
Cuts at: 2446 4485 2446
Size: 2039 2894
BlpI GC'TnA_GC
Cuts at: 816 2043 816
Size: 1227 3706
Bme1580I G_kGCm'C
Cuts at: 415 998 1630 1795 2556 2649 3707 415
Size: 583 632 165 761 93 1058 1641
Fragments arranged by size:
1641 1058 761 632 583 165 93
BmgBI CAC'GTC
Cuts at: 1290 1536 1290
Size: 246 4687
BmrI ACTGGGnnnn_n'
Cuts at: 282 297 489 2143 2224 2559 4705 282
Size: 15 192 1654 81 335 2146 510
Fragments arranged by size:
2146 1654 510 335 192 81 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 860 3298 860
Size: 2438 2495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 244 991 1138 1750 217
Size: 27 747 147 612 3400
Fragments arranged by size:
3400 747 612 147 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2107 2986 3480 3778 2107
Size: 879 494 298 3262
Fragments arranged by size:
3262 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 714 937 4637 714
Size: 223 3700 1010
Fragments arranged by size:
3700 1010 223
BsaAI yAC'GTr
Cuts at: 219 1923 2284 219
Size: 1704 361 2868
Fragments arranged by size:
2868 1704 361
BsaBI GATnn'nnATC
Cuts at: 6 1255 2445 4484 6
Size: 1249 1190 2039 455
Fragments arranged by size:
2039 1249 1190 455
BsaHI Gr'CG_yC
Cuts at: 1870 2362 2610 3314 4810 1870
Size: 492 248 704 1496 1993
Fragments arranged by size:
1993 1496 704 492 248
BsaWI w'CCGG_w
Cuts at: 1469 2120 2641 3321 3595 3742 4153 1469
Size: 651 521 680 274 147 411 2249
Fragments arranged by size:
2249 680 651 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1206 1236 1206
Size: 30 4903
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 144 205 708 1333 1360 1471 1531 126
Size: 18 61 503 625 27 111 60 3528
Fragments arranged by size:
3528 625 503 111 61 60 27 18
BseYI C'CCAG_C
Cuts at: 408 1226 1353 1548 2356 3114 3693 4189 408
Size: 818 127 195 808 758 579 496 1152
Fragments arranged by size:
1152 818 808 758 579 496 195 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4933
BsiEI CG_ry'CG
Cuts at: 104 549 1398 1819 2473 2519 3729 104
Size: 445 849 421 654 46 1210 1308
Fragments arranged by size:
1308 1210 849 654 445 421 46
BsiHKAI G_wGCw'C
Cuts at: 1622 1655 1763 1844 1945 2723 2913 3707 1622
Size: 33 108 81 101 778 190 794 2848
Fragments arranged by size:
2848 794 778 190 108 101 81 33
BsmBI CGTCTCn'nnnn_
Cuts at: 1207 1207
Size: 4933
Bsp1286I G_dGCh'C
Cuts at: 366 415 817 998 1610 1622 1630 1655 1763
Size: 49 402 181 612 12 8 25 108
Cuts at: 1763 1795 1844 1945 2556 2649 2723 2913 2975
Size: 32 49 101 611 93 74 190 62
Cuts at: 2975 3707 366
Size: 732 1592
Fragments arranged by size:
1592 732 612 611 402 190 181 108
101 93 74 62 49 49 32 25
12 8
BspEI T'CCGG_A
Cuts at: 1469 4153 1469
Size: 2684 2249
BspHI T'CATG_A
Cuts at: 4109 4109
Size: 4933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 476 1648 1981 2195 2497 2878 476
Size: 1172 333 214 302 381 2531
Fragments arranged by size:
2531 1172 381 333 302 214
BsrBI CCG'CTC
Cuts at: 50 176 1053 1134 3223 3277 4763 4928 50
Size: 126 877 81 2089 54 1486 165 55
Fragments arranged by size:
2089 1486 877 165 126 81 55 54
BsrDI GCAATG_nn'
Cuts at: 939 2843 939
Size: 1904 3029
BsrFI r'CCGG_y
Cuts at: 866 1721 2929 3110 3321 866
Size: 855 1208 181 211 2478
Fragments arranged by size:
2478 1208 855 211 181
BssSI C'ACGA_G
Cuts at: 1621 3202 3562 1621
Size: 1581 360 2992
Fragments arranged by size:
2992 1581 360
BstAPI GCAn_nnn'nTGC
Cuts at: 243 534 2308 243
Size: 291 1774 2868
Fragments arranged by size:
2868 1774 291
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4933
BstEII G'GTnAC_C
Cuts at: 754 754
Size: 4933
BstXI CCAn_nnnn'nTGG
Cuts at: 751 1314 751
Size: 563 4370
BstYI r'GATC_y
Cuts at: 1 1256 1956 2423 2446 2781 3027 4041 4127
Size: 1255 700 467 23 335 246 1014 86
Cuts at: 4127 4139 4485 1
Size: 12 346 449
Fragments arranged by size:
1255 1014 700 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 831 1645 831
Size: 814 4119
BtgI C'CryG_G
Cuts at: 125 849 879 1208 125
Size: 724 30 329 3850
Fragments arranged by size:
3850 724 329 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2881 3062 3225 2881
Size: 181 163 4589
Fragments arranged by size:
4589 181 163
BtsI GCAGTG_nn'
Cuts at: 777 977 2174 777
Size: 200 1197 3536
Fragments arranged by size:
3536 1197 200
DraI TTT'AAA
Cuts at: 1930 1930
Size: 4933
DrdI GACnn_nn'nnGTC
Cuts at: 2637 3497 2637
Size: 860 4073
EaeI y'GGCC_r
Cuts at: 428 1205 1766 2516 2690 3081 3108 4455 428
Size: 777 561 750 174 391 27 1347 906
Fragments arranged by size:
1347 906 777 750 561 391 174 27
EagI C'GGCC_G
Cuts at: 2516 2516
Size: 4933
EarI CTCTTCn'nnn_
Cuts at: 2954 3164 2954
Size: 210 4723
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3139 3451 3597 4743 3139
Size: 312 146 1146 3329
Fragments arranged by size:
3329 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1653 1761 1842 1943 1653
Size: 108 81 101 4643
Fragments arranged by size:
4643 108 101 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 244 432 991 1138 1750 2756 3188 3937
Size: 27 188 559 147 612 1006 432 749
Cuts at: 3937 4205 217
Size: 268 945
Fragments arranged by size:
1006 945 749 612 559 432 268 188
147 27
EcoNI CCTnn'n_nnAGG
Cuts at: 834 1570 1644 834
Size: 736 74 4123
Fragments arranged by size:
4123 736 74
EcoO109I rG'GnC_Cy
Cuts at: 284 465 615 1011 2070 284
Size: 181 150 396 1059 3147
Fragments arranged by size:
3147 1059 396 181 150
EcoRI G'AATT_C
Cuts at: 1935 1935
Size: 4933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 504 2950 504
Size: 2446 2487
FspI TGC'GCA
Cuts at: 1369 1591 2712 1369
Size: 222 1121 3590
Fragments arranged by size:
3590 1121 222
FspAI rTGC'GCAy
Cuts at: 1369 1369
Size: 4933
HaeII r_GCGC'y
Cuts at: 204 873 1444 1545 2365 2613 3637 4616 204
Size: 669 571 101 820 248 1024 979 521
Fragments arranged by size:
1024 979 820 669 571 521 248 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 687 719 1206 1238 2773 2803 2805 2835 687
Size: 32 487 32 1535 30 2 30 2785
Fragments arranged by size:
2785 1535 487 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1970 1970
Size: 4933
KasI G'GCGC_C
Cuts at: 2361 2609 2361
Size: 248 4685
KpnI G_GTAC'C
Cuts at: 1951 1951
Size: 4933
MluI A'CGCG_T
Cuts at: 4366 4366
Size: 4933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 537 1418 3604 3788 537
Size: 881 2186 184 1682
Fragments arranged by size:
2186 1682 881 184
MscI TGG'CCA
Cuts at: 1768 2692 1768
Size: 924 4009
MslI CAynn'nnrTG
Cuts at: 932 1312 2920 3047 932
Size: 380 1608 127 2818
Fragments arranged by size:
2818 1608 380 127
MspA1I CmG'CkG
Cuts at: 313 330 341 1417 1740 1856 2042 2356 2716
Size: 17 11 1076 323 116 186 314 360
Cuts at: 2716 3731 3976 313
Size: 1015 245 1270
Fragments arranged by size:
1270 1076 1015 360 323 314 245 186
116 17 11
NaeI GCC'GGC
Cuts at: 1723 3112 1723
Size: 1389 3544
NarI GG'CG_CC
Cuts at: 2362 2610 2362
Size: 248 4685
NcoI C'CATG_G
Cuts at: 125 849 879 125
Size: 724 30 4179
Fragments arranged by size:
4179 724 30
NgoMIV G'CCGG_C
Cuts at: 1721 3110 1721
Size: 1389 3544
NruI TCG'CGA
Cuts at: 3294 3294
Size: 4933
NspI r_CATG'y
Cuts at: 408 1272 1491 1984 2484 3393 408
Size: 864 219 493 500 909 1948
Fragments arranged by size:
1948 909 864 500 493 219
PciI A'CATG_T
Cuts at: 3389 3389
Size: 4933
PflMI CCAn_nnn'nTGG
Cuts at: 657 657
Size: 4933
PmeI GTTT'AAAC
Cuts at: 1930 1930
Size: 4933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4072 4104 4072
Size: 32 4901
PpuMI rG'GwC_Cy
Cuts at: 284 465 615 284
Size: 181 150 4602
Fragments arranged by size:
4602 181 150
PspXI vC'TCGA_Gb
Cuts at: 1006 1006
Size: 4933
PstI C_TGCA'G
Cuts at: 144 322 473 984 1496 1526 1712 1978 2663
Size: 178 151 511 512 30 186 266 685
Cuts at: 2663 144
Size: 2414
Fragments arranged by size:
2414 685 512 511 266 186 178 151
30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4933
PvuII CAG'CTG
Cuts at: 341 1740 1856 2356 2716 341
Size: 1399 116 500 360 2558
Fragments arranged by size:
2558 1399 500 360 116
RsrII CG'GwC_CG
Cuts at: 3126 3126
Size: 4933
SacI G_AGCT'C
Cuts at: 1655 1763 1844 1945 1655
Size: 108 81 101 4643
Fragments arranged by size:
4643 108 101 81
SalI G'TCGA_C
Cuts at: 1968 1968
Size: 4933
SapI GCTCTTCn'nnn_
Cuts at: 2954 3164 2954
Size: 210 4723
SbfI CC_TGCA'GG
Cuts at: 1978 1978
Size: 4933
ScaI AGT'ACT
Cuts at: 1888 4450 1888
Size: 2562 2371
SexAI A'CCwGG_T
Cuts at: 230 230
Size: 4933
SfcI C'TryA_G
Cuts at: 33 140 318 469 855 980 1492 1522 1708
Size: 107 178 151 386 125 512 30 186
Cuts at: 1708 1974 2659 3654 3845 33
Size: 266 685 995 191 1121
Fragments arranged by size:
1121 995 685 512 386 266 191 186
178 151 125 107 30
SfoI GGC'GCC
Cuts at: 2363 2611 2363
Size: 248 4685
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4933
SmaI CCC'GGG
Cuts at: 1953 1953
Size: 4933
SmlI C'TyrA_G
Cuts at: 1006 2086 3001 3495 3757 1006
Size: 1080 915 494 262 2182
Fragments arranged by size:
2182 1080 915 494 262
SnaBI TAC'GTA
Cuts at: 1923 1923
Size: 4933
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4933
SphI G_CATG'C
Cuts at: 1272 1984 2484 1272
Size: 712 500 3721
Fragments arranged by size:
3721 712 500
StyI C'CwwG_G
Cuts at: 125 849 879 2065 2419 125
Size: 724 30 1186 354 2639
Fragments arranged by size:
2639 1186 724 354 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 508 911 2514 2647 3106 3317 508
Size: 403 1603 133 459 211 2124
Fragments arranged by size:
2124 1603 459 403 211 133
TatI w'GTAC_w
Cuts at: 1714 1886 4448 1714
Size: 172 2562 2199
Fragments arranged by size:
2562 2199 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 784 816 4450 4482 784
Size: 32 3634 32 1235
Fragments arranged by size:
3634 1235 32 32
Tth111I GACn'n_nGTC
Cuts at: 288 1815 2728 288
Size: 1527 913 2493
Fragments arranged by size:
2493 1527 913
XbaI T'CTAG_A
Cuts at: 1962 1962
Size: 4933
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 2426
Size: 4933
XhoI C'TCGA_G
Cuts at: 1006 1006
Size: 4933
XmaI C'CCGG_G
Cuts at: 1951 1951
Size: 4933
XmnI GAAnn'nnTTC
Cuts at: 682 1501 682
Size: 819 4114
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PsrI
SacII SanDI SfiI SgrAI SrfI SspI StuI SwaI
ZraI