Restriction map for
pF1KSDA0388
(Circular) (Six-base) MAPSORT of: pF1KSDA0388.seq Check: 5387 from: 1 to: 5380
pF1KSDA0388 5380 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1735 1976 1735
Size: 241 5139
AatII G_ACGT'C
Cuts at: 2363 2363
Size: 5380
AccI GT'mk_AC
Cuts at: 2416 2416
Size: 5380
Acc65I G'GTAC_C
Cuts at: 2394 2394
Size: 5380
AclI AA'CG_TT
Cuts at: 5220 5220
Size: 5380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 819 1310 1457 1582 1672 3203 3635 4384
Size: 614 491 147 125 90 1531 432 749
Cuts at: 4384 4652 205
Size: 268 933
Fragments arranged by size:
1531 933 749 614 491 432 268 147
125 90
AflII C'TTAA_G
Cuts at: 1957 1957
Size: 5380
AflIII A'CryG_T
Cuts at: 2111 2730 3836 4813 2111
Size: 619 1106 977 2678
Fragments arranged by size:
2678 1106 977 619
AgeI A'CCGG_T
Cuts at: 3768 3768
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1675 1675
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 1258 1727 2683 4252 1258
Size: 469 956 1569 2386
Fragments arranged by size:
2386 1569 956 469
ApaLI G'TGCA_C
Cuts at: 942 4150 942
Size: 3208 2172
ApoI r'AATT_y
Cuts at: 258 1749 2035 2177 2382 4733 4989 258
Size: 1491 286 142 205 2351 256 649
Fragments arranged by size:
2351 1491 649 286 256 205 142
AseI AT'TA_AT
Cuts at: 20 20
Size: 5380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5380
AvaI C'yCGr_G
Cuts at: 393 2398 393
Size: 2005 3375
BamHI G'GATC_C
Cuts at: 625 2403 625
Size: 1778 3602
BanI G'GyrC_C
Cuts at: 1217 1465 2002 2394 2808 3056 3091 1217
Size: 248 537 392 414 248 35 3506
Fragments arranged by size:
3506 537 414 392 248 248 35
BanII G_rGCy'C
Cuts at: 1583 2063 2294 2392 3422 1583
Size: 480 231 98 1030 3541
Fragments arranged by size:
3541 1030 480 231 98
BbeI G_GCGC'C
Cuts at: 2812 3060 2812
Size: 248 5132
BbsI GAAGACnn'nnnn_
Cuts at: 1449 1449
Size: 5380
BbvCI CC'TCA_GC
Cuts at: 1251 1251
Size: 5380
BciVI GTATCCnnnnn_n'
Cuts at: 3276 4039 5380 3276
Size: 763 1341 3276
Fragments arranged by size:
3276 1341 763
BclI T'GATC_A
Cuts at: 575 2898 575
Size: 2323 3057
BglI GCCn_nnn'nGGC
Cuts at: 2269 2269
Size: 5380
BglII A'GATC_T
Cuts at: 747 2893 4932 747
Size: 2146 2039 1195
Fragments arranged by size:
2146 2039 1195
BlpI GC'TnA_GC
Cuts at: 2490 2490
Size: 5380
Bme1580I G_kGCm'C
Cuts at: 707 946 2005 3003 3096 4154 707
Size: 239 1059 998 93 1058 1933
Fragments arranged by size:
1933 1059 1058 998 239 93
BmgBI CAC'GTC
Cuts at: 1517 1517
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 1320 1439 1815 1917 2590 2671 3006 5152 1320
Size: 119 376 102 673 81 335 2146 1548
Fragments arranged by size:
2146 1548 673 376 335 119 102 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1353 2082 3745 1353
Size: 729 1663 2988
Fragments arranged by size:
2988 1663 729
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1302 676
Size: 626 4754
Bpu10I CC'TnA_GC
Cuts at: 1251 1251
Size: 5380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2314 2554 3433 3927 4225 2314
Size: 240 879 494 298 3469
Fragments arranged by size:
3469 879 494 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 2234 2347 5084 2234
Size: 113 2737 2530
Fragments arranged by size:
2737 2530 113
BsaAI yAC'GTr
Cuts at: 2337 2370 2731 2337
Size: 33 361 4986
Fragments arranged by size:
4986 361 33
BsaBI GATnn'nnATC
Cuts at: 6 2254 2892 4931 6
Size: 2248 638 2039 455
Fragments arranged by size:
2248 2039 638 455
BsaHI Gr'CG_yC
Cuts at: 2360 2809 3057 3761 5257 2360
Size: 449 248 704 1496 2483
Fragments arranged by size:
2483 1496 704 449 248
BsaWI w'CCGG_w
Cuts at: 2567 3088 3768 4042 4189 4600 2567
Size: 521 680 274 147 411 3347
Fragments arranged by size:
3347 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 524 554 1171 1201 1300 1330 524
Size: 30 617 30 99 30 4574
Fragments arranged by size:
4574 617 99 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 706 709 583
Size: 123 3 5254
Fragments arranged by size:
5254 123 3
BseYI C'CCAG_C
Cuts at: 2803 3561 4140 4636 2803
Size: 758 579 496 3547
Fragments arranged by size:
3547 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1000 1613 2044 982
Size: 18 613 431 4318
Fragments arranged by size:
4318 613 431 18
BsiEI CG_ry'CG
Cuts at: 104 2920 2966 4176 104
Size: 2816 46 1210 1308
Fragments arranged by size:
2816 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 946 1198 1583 1907 2063 2294 2392 3170 3360
Size: 252 385 324 156 231 98 778 190
Cuts at: 3360 4154 946
Size: 794 2172
Fragments arranged by size:
2172 794 778 385 324 252 231 190
156 98
BsmBI CGTCTCn'nnnn_
Cuts at: 496 496
Size: 5380
Bsp1286I G_dGCh'C
Cuts at: 707 946 1198 1583 1907 2005 2063 2294 2392
Size: 239 252 385 324 98 58 231 98
Cuts at: 2392 3003 3096 3170 3360 3422 4154 707
Size: 611 93 74 190 62 732 1933
Fragments arranged by size:
1933 732 611 385 324 252 239 231
190 98 98 93 74 62 58
BspEI T'CCGG_A
Cuts at: 4600 4600
Size: 5380
BspHI T'CATG_A
Cuts at: 1583 1739 4556 1583
Size: 156 2817 2407
Fragments arranged by size:
2817 2407 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1515 1735 1976 2428 2642 2944 3325 1515
Size: 220 241 452 214 302 381 3570
Fragments arranged by size:
3570 452 381 302 241 220 214
BsrBI CCG'CTC
Cuts at: 50 1187 3670 3724 5210 5375 50
Size: 1137 2483 54 1486 165 55
Fragments arranged by size:
2483 1486 1137 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1311 1843 3290 1311
Size: 532 1447 3401
Fragments arranged by size:
3401 1447 532
BsrFI r'CCGG_y
Cuts at: 1022 3376 3557 3768 1022
Size: 2354 181 211 2634
Fragments arranged by size:
2634 2354 211 181
BsrGI T'GTAC_A
Cuts at: 1679 1679
Size: 5380
BssSI C'ACGA_G
Cuts at: 3649 4009 3649
Size: 360 5020
BstAPI GCAn_nnn'nTGC
Cuts at: 262 996 1133 1971 2755 262
Size: 734 137 838 784 2887
Fragments arranged by size:
2887 838 784 734 137
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5380
BstXI CCAn_nnnn'nTGG
Cuts at: 1503 2251 1503
Size: 748 4632
BstYI r'GATC_y
Cuts at: 1 287 625 747 2255 2403 2870 2893 3228
Size: 286 338 122 1508 148 467 23 335
Cuts at: 3228 3474 4488 4574 4586 4932 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1508 1014 467 449 346 338 335 286
246 148 122 86 23 12
BtgI C'CryG_G
Cuts at: 125 600 1461 125
Size: 475 861 4044
Fragments arranged by size:
4044 861 475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3328 3509 3672 3328
Size: 181 163 5036
Fragments arranged by size:
5036 181 163
BtsI GCAGTG_nn'
Cuts at: 1632 1862 2621 1632
Size: 230 759 4391
Fragments arranged by size:
4391 759 230
ClaI AT'CG_AT
Cuts at: 956 956
Size: 5380
DraI TTT'AAA
Cuts at: 1035 2377 1035
Size: 1342 4038
DrdI GACnn_nn'nnGTC
Cuts at: 3084 3944 3084
Size: 860 4520
EaeI y'GGCC_r
Cuts at: 1991 2963 3137 3528 3555 4902 1991
Size: 972 174 391 27 1347 2469
Fragments arranged by size:
2469 1347 972 391 174 27
EagI C'GGCC_G
Cuts at: 2963 2963
Size: 5380
EarI CTCTTCn'nnn_
Cuts at: 605 1671 2131 2282 2299 3401 3611 605
Size: 1066 460 151 17 1102 210 2374
Fragments arranged by size:
2374 1102 1066 460 210 151 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3586 3898 4044 5190 3586
Size: 312 146 1146 3776
Fragments arranged by size:
3776 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1581 2061 2292 2390 1581
Size: 480 231 98 4571
Fragments arranged by size:
4571 480 231 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 676 819 1302 1310 1457 1582 1672 3203
Size: 471 143 483 8 147 125 90 1531
Cuts at: 3203 3635 4384 4652 205
Size: 432 749 268 933
Fragments arranged by size:
1531 933 749 483 471 432 268 147
143 125 90 8
EcoO109I rG'GnC_Cy
Cuts at: 1895 2517 1895
Size: 622 4758
EcoRI G'AATT_C
Cuts at: 2035 2382 2035
Size: 347 5033
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1462 1545 1570 3397 1462
Size: 83 25 1827 3445
Fragments arranged by size:
3445 1827 83 25
FspI TGC'GCA
Cuts at: 3159 3159
Size: 5380
HaeII r_GCGC'y
Cuts at: 1029 2812 3060 4084 5063 1029
Size: 1783 248 1024 979 1346
Fragments arranged by size:
1783 1346 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1300 1332 3220 3250 3252 3282 1300
Size: 32 1888 30 2 30 3398
Fragments arranged by size:
3398 1888 32 30 30 2
HincII GTy'rAC
Cuts at: 2417 2417
Size: 5380
HindIII A'AGCT_T
Cuts at: 310 310
Size: 5380
KasI G'GCGC_C
Cuts at: 2808 3056 2808
Size: 248 5132
KpnI G_GTAC'C
Cuts at: 2398 2398
Size: 5380
MfeI C'AATT_G
Cuts at: 842 842
Size: 5380
MluI A'CGCG_T
Cuts at: 4813 4813
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 345 501 4051 4235 345
Size: 156 3550 184 1490
Fragments arranged by size:
3550 1490 184 156
MscI TGG'CCA
Cuts at: 3139 3139
Size: 5380
MslI CAynn'nnrTG
Cuts at: 1675 3367 3494 1675
Size: 1692 127 3561
Fragments arranged by size:
3561 1692 127
MspA1I CmG'CkG
Cuts at: 984 1650 2489 2803 3163 4178 4423 984
Size: 666 839 314 360 1015 245 1941
Fragments arranged by size:
1941 1015 839 666 360 314 245
NaeI GCC'GGC
Cuts at: 1024 3559 1024
Size: 2535 2845
NarI GG'CG_CC
Cuts at: 2809 3057 2809
Size: 248 5132
NcoI C'CATG_G
Cuts at: 125 600 1461 125
Size: 475 861 4044
Fragments arranged by size:
4044 861 475
NdeI CA'TA_TG
Cuts at: 413 413
Size: 5380
NgoMIV G'CCGG_C
Cuts at: 1022 3557 1022
Size: 2535 2845
NruI TCG'CGA
Cuts at: 3741 3741
Size: 5380
NspI r_CATG'y
Cuts at: 198 781 2115 2431 2931 3840 198
Size: 583 1334 316 500 909 1738
Fragments arranged by size:
1738 1334 909 583 500 316
PciI A'CATG_T
Cuts at: 2111 3836 2111
Size: 1725 3655
PmeI GTTT'AAAC
Cuts at: 2377 2377
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4519 4551 4519
Size: 32 5348
PshAI GACnn'nnGTC
Cuts at: 1880 2359 1880
Size: 479 4901
PstI C_TGCA'G
Cuts at: 1639 1942 2425 3110 1639
Size: 303 483 685 3909
Fragments arranged by size:
3909 685 483 303
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5380
PvuII CAG'CTG
Cuts at: 1650 2803 3163 1650
Size: 1153 360 3867
Fragments arranged by size:
3867 1153 360
RsrII CG'GwC_CG
Cuts at: 3573 3573
Size: 5380
SacI G_AGCT'C
Cuts at: 1583 2063 2294 2392 1583
Size: 480 231 98 4571
Fragments arranged by size:
4571 480 231 98
SalI G'TCGA_C
Cuts at: 2415 2415
Size: 5380
SapI GCTCTTCn'nnn_
Cuts at: 2282 2299 3401 3611 2282
Size: 17 1102 210 4051
Fragments arranged by size:
4051 1102 210 17
SbfI CC_TGCA'GG
Cuts at: 2425 2425
Size: 5380
ScaI AGT'ACT
Cuts at: 682 4897 682
Size: 4215 1165
SfcI C'TryA_G
Cuts at: 33 1635 1938 2421 3106 4101 4292 33
Size: 1602 303 483 685 995 191 1121
Fragments arranged by size:
1602 1121 995 685 483 303 191
SfoI GGC'GCC
Cuts at: 2810 3058 2810
Size: 248 5132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5380
SmaI CCC'GGG
Cuts at: 395 2400 395
Size: 2005 3375
SmlI C'TyrA_G
Cuts at: 1957 2329 2533 3448 3942 4204 1957
Size: 372 204 915 494 262 3133
Fragments arranged by size:
3133 915 494 372 262 204
SnaBI TAC'GTA
Cuts at: 2370 2370
Size: 5380
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5380
SphI G_CATG'C
Cuts at: 2431 2931 2431
Size: 500 4880
SspI AAT'ATT
Cuts at: 519 519
Size: 5380
StyI C'CwwG_G
Cuts at: 125 600 1461 2512 2866 125
Size: 475 861 1051 354 2639
Fragments arranged by size:
2639 1051 861 475 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 223 963 1718 2371 2961 3094 3553 3764 223
Size: 740 755 653 590 133 459 211 1839
Fragments arranged by size:
1839 755 740 653 590 459 211 133
TatI w'GTAC_w
Cuts at: 680 1679 4895 680
Size: 999 3216 1165
Fragments arranged by size:
3216 1165 999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 4897 4929 524
Size: 32 4341 32 975
Fragments arranged by size:
4341 975 32 32
Tth111I GACn'n_nGTC
Cuts at: 3175 3175
Size: 5380
XbaI T'CTAG_A
Cuts at: 2409 2409
Size: 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2226 2873 2226
Size: 647 4733
XmaI C'CCGG_G
Cuts at: 393 2398 393
Size: 2005 3375
XmnI GAAnn'nnTTC
Cuts at: 814 1795 2035 814
Size: 981 240 4159
Fragments arranged by size:
4159 981 240
ZraI GAC'GTC
Cuts at: 2361 2361
Size: 5380
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PciI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NheI NotI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XhoI