Restriction map for
pF1KSDA0364
(Circular) (Six-base) MAPSORT of: pF1KSDA0364.seq Check: 7528 from: 1 to: 7120
pF1KSDA0364 7120 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 4156 4156
Size: 7120
Acc65I G'GTAC_C
Cuts at: 4134 4134
Size: 7120
AclI AA'CG_TT
Cuts at: 6960 6960
Size: 7120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 331 769 1399 1624 1993 3337 3625 4943
Size: 147 438 630 225 369 1344 288 1318
Cuts at: 4943 5375 6124 6392 184
Size: 432 749 268 912
Fragments arranged by size:
1344 1318 912 749 630 438 432 369
288 268 225 147
AflIII A'CryG_T
Cuts at: 4470 5576 6553 4470
Size: 1106 977 5037
Fragments arranged by size:
5037 1106 977
AgeI A'CCGG_T
Cuts at: 410 5508 410
Size: 5098 2022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 1703 2294 319
Size: 1384 591 5145
Fragments arranged by size:
5145 1384 591
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 2819 2851 2183
Size: 32 604 32 6452
Fragments arranged by size:
6452 604 32 32
AlwNI CAG_nnn'CTG
Cuts at: 610 2567 2746 3212 3853 4423 5992 610
Size: 1957 179 466 641 570 1569 1738
Fragments arranged by size:
1957 1738 1569 641 570 466 179
ApaI G_GGCC'C
Cuts at: 806 806
Size: 7120
ApaLI G'TGCA_C
Cuts at: 3867 5890 3867
Size: 2023 5097
ApoI r'AATT_y
Cuts at: 936 1483 2707 3670 4122 6473 6729 936
Size: 547 1224 963 452 2351 256 1327
Fragments arranged by size:
2351 1327 1224 963 547 452 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7120
AvaI C'yCGr_G
Cuts at: 2477 4138 2477
Size: 1661 5459
BamHI G'GATC_C
Cuts at: 335 3142 4143 335
Size: 2807 1001 3312
Fragments arranged by size:
3312 2807 1001
BanI G'GyrC_C
Cuts at: 269 379 471 813 839 2041 2473 3068 4042
Size: 110 92 342 26 1202 432 595 974
Cuts at: 4042 4134 4548 4796 4831 269
Size: 92 414 248 35 2558
Fragments arranged by size:
2558 1202 974 595 432 414 342 248
110 92 92 35 26
BanII G_rGCy'C
Cuts at: 737 806 1601 2111 2390 2678 2696 2832 3029
Size: 69 795 510 279 288 18 136 197
Cuts at: 3029 3408 4132 5162 737
Size: 379 724 1030 2695
Fragments arranged by size:
2695 1030 795 724 510 379 288 279
197 136 69 18
BbeI G_GCGC'C
Cuts at: 2045 4552 4800 2045
Size: 2507 248 4365
Fragments arranged by size:
4365 2507 248
BbsI GAAGACnn'nnnn_
Cuts at: 172 893 922 1181 1300 3485 172
Size: 721 29 259 119 2185 3807
Fragments arranged by size:
3807 2185 721 259 119 29
BbvCI CC'TCA_GC
Cuts at: 3704 3704
Size: 7120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2790 2824 2790
Size: 34 7086
BciVI GTATCCnnnnn_n'
Cuts at: 3088 5016 5779 7120 3088
Size: 1928 763 1341 3088
Fragments arranged by size:
3088 1928 1341 763
BclI T'GATC_A
Cuts at: 4638 4638
Size: 7120
BglI GCCn_nnn'nGGC
Cuts at: 1946 1946
Size: 7120
BglII A'GATC_T
Cuts at: 3557 4633 6672 3557
Size: 1076 2039 4005
Fragments arranged by size:
4005 2039 1076
BlpI GC'TnA_GC
Cuts at: 3023 4230 3023
Size: 1207 5913
Bme1580I G_kGCm'C
Cuts at: 384 806 2478 3871 4743 4836 5894 384
Size: 422 1672 1393 872 93 1058 1610
Fragments arranged by size:
1672 1610 1393 1058 872 422 93
BmrI ACTGGGnnnn_n'
Cuts at: 443 641 1079 2105 2384 3023 4330 4411 4746
Size: 198 438 1026 279 639 1307 81 335
Cuts at: 4746 6892 443
Size: 2146 671
Fragments arranged by size:
2146 1307 1026 671 639 438 335 279
198 81
BmtI G_CTAG'C
Cuts at: 3937 3937
Size: 7120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1033 5485 1033
Size: 4452 2668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 1462 2422 3574 3772 3850 839
Size: 623 960 1152 198 78 4109
Fragments arranged by size:
4109 1152 960 623 198 78
Bpu10I CC'TnA_GC
Cuts at: 1868 1874 2429 3005 3407 3704 3860 1868
Size: 6 555 576 402 297 156 5128
Fragments arranged by size:
5128 576 555 402 297 156 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1754 2143 4294 5173 5667 5965 1754
Size: 389 2151 879 494 298 2909
Fragments arranged by size:
2909 2151 879 494 389 298
BsaI GGTCTCn'nnnn_
Cuts at: 508 1882 3989 6824 508
Size: 1374 2107 2835 804
Fragments arranged by size:
2835 2107 1374 804
BsaAI yAC'GTr
Cuts at: 4110 4471 4110
Size: 361 6759
BsaBI GATnn'nnATC
Cuts at: 6 4632 6671 6
Size: 4626 2039 455
Fragments arranged by size:
4626 2039 455
BsaHI Gr'CG_yC
Cuts at: 2042 4549 4797 5501 6997 2042
Size: 2507 248 704 1496 2165
Fragments arranged by size:
2507 2165 1496 704 248
BsaWI w'CCGG_w
Cuts at: 210 410 2201 4307 4828 5508 5782 5929 6340
Size: 200 1791 2106 521 680 274 147 411
Cuts at: 6340 210
Size: 990
Fragments arranged by size:
2106 1791 990 680 521 411 274 200
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1951 1981 2183 2213 2711 2741 2819 2849 3532
Size: 30 202 30 498 30 78 30 683
Cuts at: 3532 3562 3716 3746 1951
Size: 30 154 30 5325
Fragments arranged by size:
5325 683 498 202 154 78 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2104 2104
Size: 7120
BseYI C'CCAG_C
Cuts at: 736 1758 2677 3298 3311 3604 4543 5301 5880
Size: 1022 919 621 13 293 939 758 579
Cuts at: 5880 6376 736
Size: 496 1480
Fragments arranged by size:
1480 1022 939 919 758 621 579 496
293 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1953 2609 3088 1953
Size: 656 479 5985
Fragments arranged by size:
5985 656 479
BsiEI CG_ry'CG
Cuts at: 104 4660 4706 5916 104
Size: 4556 46 1210 1308
Fragments arranged by size:
4556 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2696 3871 4132 4910 5100 5894 2696
Size: 1175 261 778 190 794 3922
Fragments arranged by size:
3922 1175 794 778 261 190
BsmI GAATG_Cn'
Cuts at: 191 3163 191
Size: 2972 4148
Bsp1286I G_dGCh'C
Cuts at: 384 737 806 1601 2111 2390 2478 2678 2696
Size: 353 69 795 510 279 88 200 18
Cuts at: 2696 2832 3029 3408 3871 4132 4743 4836 4910
Size: 136 197 379 463 261 611 93 74
Cuts at: 4910 5100 5162 5894 384
Size: 190 62 732 1610
Fragments arranged by size:
1610 795 732 611 510 463 379 353
279 261 200 197 190 136 93 88
74 69 62 18
BspEI T'CCGG_A
Cuts at: 6340 6340
Size: 7120
BspHI T'CATG_A
Cuts at: 6296 6296
Size: 7120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 391 2007 4168 4382 4684 5065 391
Size: 1616 2161 214 302 381 2446
Fragments arranged by size:
2446 2161 1616 381 302 214
BsrBI CCG'CTC
Cuts at: 50 523 5410 5464 6950 7115 50
Size: 473 4887 54 1486 165 55
Fragments arranged by size:
4887 1486 473 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1830 4004 5030 1830
Size: 2174 1026 3920
Fragments arranged by size:
3920 2174 1026
BsrFI r'CCGG_y
Cuts at: 410 3078 5116 5297 5508 410
Size: 2668 2038 181 211 2022
Fragments arranged by size:
2668 2038 2022 211 181
BssSI C'ACGA_G
Cuts at: 2695 5389 5749 2695
Size: 2694 360 4066
Fragments arranged by size:
4066 2694 360
BstAPI GCAn_nnn'nTGC
Cuts at: 4495 4495
Size: 7120
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7120
BstEII G'GTnAC_C
Cuts at: 1776 2463 1776
Size: 687 6433
BstXI CCAn_nnnn'nTGG
Cuts at: 132 970 1092 2275 2460 2730 2893 3036 132
Size: 838 122 1183 185 270 163 143 4216
Fragments arranged by size:
4216 1183 838 270 185 163 143 122
BstYI r'GATC_y
Cuts at: 1 335 959 3142 3488 3557 4143 4610 4633
Size: 334 624 2183 346 69 586 467 23
Cuts at: 4633 4968 5214 6228 6314 6326 6672 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
2183 1014 624 586 467 449 346 346
335 334 246 86 69 23 12
Bsu36I CC'TnA_GG
Cuts at: 671 671
Size: 7120
BtgI C'CryG_G
Cuts at: 555 1909 3010 3044 3084 3323 555
Size: 1354 1101 34 40 239 4352
Fragments arranged by size:
4352 1354 1101 239 40 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1573 5068 5249 5412 1573
Size: 3495 181 163 3281
Fragments arranged by size:
3495 3281 181 163
BtsI GCAGTG_nn'
Cuts at: 1599 2073 3473 4361 1599
Size: 474 1400 888 4358
Fragments arranged by size:
4358 1400 888 474
ClaI AT'CG_AT
Cuts at: 1211 1211
Size: 7120
DraI TTT'AAA
Cuts at: 591 4117 591
Size: 3526 3594
DraIII CAC_nnn'GTG
Cuts at: 725 725
Size: 7120
DrdI GACnn_nn'nnGTC
Cuts at: 438 2943 4824 5684 438
Size: 2505 1881 860 1874
Fragments arranged by size:
2505 1881 1874 860
EaeI y'GGCC_r
Cuts at: 641 1375 4703 4877 5268 5295 6642 641
Size: 734 3328 174 391 27 1347 1119
Fragments arranged by size:
3328 1347 1119 734 391 174 27
EagI C'GGCC_G
Cuts at: 4703 4703
Size: 7120
EarI CTCTTCn'nnn_
Cuts at: 530 1792 1835 2283 2758 3356 3987 4010 5141
Size: 1262 43 448 475 598 631 23 1131
Cuts at: 5141 5351 530
Size: 210 2299
Fragments arranged by size:
2299 1262 1131 631 598 475 448 210
43 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 328 5326 5638 5784 6930 328
Size: 4998 312 146 1146 518
Fragments arranged by size:
4998 1146 518 312 146
EcoICRI GAG'CTC
Cuts at: 2694 4130 2694
Size: 1436 5684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 331 769 839 1399 1462 1624 1993 2422
Size: 147 438 70 560 63 162 369 429
Cuts at: 2422 3337 3574 3625 3772 3850 4943 5375 6124
Size: 915 237 51 147 78 1093 432 749
Cuts at: 6124 6392 184
Size: 268 912
Fragments arranged by size:
1093 915 912 749 560 438 432 429
369 268 237 162 147 147 78 70
63 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1302 2818 3394 1302
Size: 1516 576 5028
Fragments arranged by size:
5028 1516 576
EcoO109I rG'GnC_Cy
Cuts at: 243 1538 2564 2743 3652 3850 4257 243
Size: 1295 1026 179 909 198 407 3106
Fragments arranged by size:
3106 1295 1026 909 407 198 179
EcoRI G'AATT_C
Cuts at: 2707 4122 2707
Size: 1415 5705
EcoRV GAT'ATC
Cuts at: 290 3804 290
Size: 3514 3606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 513 2454 5137 513
Size: 1941 2683 2496
Fragments arranged by size:
2683 2496 1941
FspI TGC'GCA
Cuts at: 4899 4899
Size: 7120
HaeII r_GCGC'y
Cuts at: 2045 4552 4800 5824 6803 2045
Size: 2507 248 1024 979 2362
Fragments arranged by size:
2507 2362 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 507 539 780 812 855 887 965
Size: 32 286 32 241 32 43 32 78
Cuts at: 965 997 1207 1239 1490 1522 1724 1756 1983
Size: 32 210 32 251 32 202 32 227
Cuts at: 1983 2015 3065 3097 3716 3748 3793 3825 4960
Size: 32 1050 32 619 32 45 32 1135
Cuts at: 4960 4990 4992 5022 189
Size: 30 2 30 2287
Fragments arranged by size:
2287 1135 1050 619 286 251 241 227
210 202 78 45 43 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 543 1521 4157 543
Size: 978 2636 3506
Fragments arranged by size:
3506 2636 978
HpaI GTT'AAC
Cuts at: 543 543
Size: 7120
KasI G'GCGC_C
Cuts at: 2041 4548 4796 2041
Size: 2507 248 4365
Fragments arranged by size:
4365 2507 248
KpnI G_GTAC'C
Cuts at: 4138 4138
Size: 7120
MfeI C'AATT_G
Cuts at: 2490 2490
Size: 7120
MluI A'CGCG_T
Cuts at: 6553 6553
Size: 7120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 254 446 1708 2265 2874 5791 5975 254
Size: 192 1262 557 609 2917 184 1399
Fragments arranged by size:
2917 1399 1262 609 557 192 184
MscI TGG'CCA
Cuts at: 643 1377 4879 643
Size: 734 3502 2884
Fragments arranged by size:
3502 2884 734
MslI CAynn'nnrTG
Cuts at: 196 1306 1592 2611 3127 5107 5234 196
Size: 1110 286 1019 516 1980 127 2082
Fragments arranged by size:
2082 1980 1110 1019 516 286 127
MspA1I CmG'CkG
Cuts at: 2345 2400 2642 2872 3209 3773 4229 4543 4903
Size: 55 242 230 337 564 456 314 360
Cuts at: 4903 5918 6163 2345
Size: 1015 245 3302
Fragments arranged by size:
3302 1015 564 456 360 337 314 245
242 230 55
NaeI GCC'GGC
Cuts at: 5299 5299
Size: 7120
NarI GG'CG_CC
Cuts at: 2042 4549 4797 2042
Size: 2507 248 4365
Fragments arranged by size:
4365 2507 248
NcoI C'CATG_G
Cuts at: 555 3010 3044 3084 3323 555
Size: 2455 34 40 239 4352
Fragments arranged by size:
4352 2455 239 40 34
NdeI CA'TA_TG
Cuts at: 2513 2513
Size: 7120
NgoMIV G'CCGG_C
Cuts at: 5297 5297
Size: 7120
NheI G'CTAG_C
Cuts at: 3933 3933
Size: 7120
NruI TCG'CGA
Cuts at: 5481 5481
Size: 7120
NspI r_CATG'y
Cuts at: 4171 4671 5580 4171
Size: 500 909 5711
Fragments arranged by size:
5711 909 500
PciI A'CATG_T
Cuts at: 5576 5576
Size: 7120
PflMI CCAn_nnn'nTGG
Cuts at: 500 725 856 1067 1141 2316 3944 500
Size: 225 131 211 74 1175 1628 3676
Fragments arranged by size:
3676 1628 1175 225 211 131 74
PmeI GTTT'AAAC
Cuts at: 4117 4117
Size: 7120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2183 2215 2819 2851 6259 6291 2183
Size: 32 604 32 3408 32 3012
Fragments arranged by size:
3408 3012 604 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1538 2564 2743 3652 1538
Size: 1026 179 909 5006
Fragments arranged by size:
5006 1026 909 179
PspOMI G'GGCC_C
Cuts at: 802 802
Size: 7120
PstI C_TGCA'G
Cuts at: 569 3778 4165 4850 569
Size: 3209 387 685 2839
Fragments arranged by size:
3209 2839 685 387
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7120
PvuII CAG'CTG
Cuts at: 2345 2642 2872 3209 3773 4543 4903 2345
Size: 297 230 337 564 770 360 4562
Fragments arranged by size:
4562 770 564 360 337 297 230
RsrII CG'GwC_CG
Cuts at: 5313 5313
Size: 7120
SacI G_AGCT'C
Cuts at: 2696 4132 2696
Size: 1436 5684
SalI G'TCGA_C
Cuts at: 4155 4155
Size: 7120
SapI GCTCTTCn'nnn_
Cuts at: 530 1792 5141 5351 530
Size: 1262 3349 210 2299
Fragments arranged by size:
3349 2299 1262 210
SbfI CC_TGCA'GG
Cuts at: 4165 4165
Size: 7120
ScaI AGT'ACT
Cuts at: 1439 6637 1439
Size: 5198 1922
SfcI C'TryA_G
Cuts at: 33 565 1248 1266 1542 2916 3492 3768 3774
Size: 532 683 18 276 1374 576 276 6
Cuts at: 3774 3872 4161 4846 5841 6032 33
Size: 98 289 685 995 191 1121
Fragments arranged by size:
1374 1121 995 685 683 576 532 289
276 276 191 98 18 6
SfoI GGC'GCC
Cuts at: 2043 4550 4798 2043
Size: 2507 248 4365
Fragments arranged by size:
4365 2507 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7120
SmaI CCC'GGG
Cuts at: 4140 4140
Size: 7120
SmlI C'TyrA_G
Cuts at: 1769 2122 4273 5188 5682 5944 1769
Size: 353 2151 915 494 262 2945
Fragments arranged by size:
2945 2151 915 494 353 262
SnaBI TAC'GTA
Cuts at: 4110 4110
Size: 7120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7120
SphI G_CATG'C
Cuts at: 4171 4671 4171
Size: 500 6620
StuI AGG'CCT
Cuts at: 3793 3793
Size: 7120
StyI C'CwwG_G
Cuts at: 247 555 843 2037 2784 3010 3044 3084 3323
Size: 308 288 1194 747 226 34 40 239
Cuts at: 3323 3920 4252 4606 247
Size: 597 332 354 2761
Fragments arranged by size:
2761 1194 747 597 354 332 308 288
239 226 40 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 521 2976 3552 4701 4834 5293 5504 521
Size: 2455 576 1149 133 459 211 2137
Fragments arranged by size:
2455 2137 1149 576 459 211 133
TatI w'GTAC_w
Cuts at: 1437 6635 1437
Size: 5198 1922
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3532 3564 6637 6669 3532
Size: 32 3073 32 3983
Fragments arranged by size:
3983 3073 32 32
Tth111I GACn'n_nGTC
Cuts at: 3730 4915 3730
Size: 1185 5935
XbaI T'CTAG_A
Cuts at: 459 3678 4149 459
Size: 3219 471 3430
Fragments arranged by size:
3430 3219 471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 628 1899 2397 2685 4613 628
Size: 1271 498 288 1928 3135
Fragments arranged by size:
3135 1928 1271 498 288
XmaI C'CCGG_G
Cuts at: 4138 4138
Size: 7120
XmnI GAAnn'nnTTC
Cuts at: 169 1941 3674 169
Size: 1772 1733 3615
Fragments arranged by size:
3615 1772 1733
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NruI NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstZ17I
CspCI FseI FspAI HindIII NotI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI XhoI ZraI