Restriction map for
pF1KSDA0362
(Circular) (Six-base) MAPSORT of: pF1KSDA0362.seq Check: 9559 from: 1 to: 6427
pF1KSDA0362 6427 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 292 1310 292
Size: 1018 5409
AatII G_ACGT'C
Cuts at: 926 1534 926
Size: 608 5819
AccI GT'mk_AC
Cuts at: 931 1412 2900 3463 931
Size: 481 1488 563 3895
Fragments arranged by size:
3895 1488 563 481
Acc65I G'GTAC_C
Cuts at: 3441 3441
Size: 6427
AclI AA'CG_TT
Cuts at: 6267 6267
Size: 6427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 1816 2449 2569 4250 4682 5431 5699 426
Size: 1390 633 120 1681 432 749 268 1154
Fragments arranged by size:
1681 1390 1154 749 633 432 268 120
AfeI AGC'GCT
Cuts at: 370 370
Size: 6427
AflIII A'CryG_T
Cuts at: 3777 4883 5860 3777
Size: 1106 977 4344
Fragments arranged by size:
4344 1106 977
AgeI A'CCGG_T
Cuts at: 1648 4815 1648
Size: 3167 3260
AhdI GACnn_n'nnGTC
Cuts at: 927 1242 927
Size: 315 6112
AleI CACnn'nnGTG
Cuts at: 1451 2677 3319 1451
Size: 1226 642 4559
Fragments arranged by size:
4559 1226 642
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 448 480 1756 1788 1897 1929 448
Size: 32 1276 32 109 32 4946
Fragments arranged by size:
4946 1276 109 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 876 1173 1185 2463 2495 2648 3030 3730 5299
Size: 297 12 1278 32 153 382 700 1569
Cuts at: 5299 876
Size: 2004
Fragments arranged by size:
2004 1569 1278 700 382 297 153 32
12
ApaI G_GGCC'C
Cuts at: 1359 1365 1943 3289 1359
Size: 6 578 1346 4497
Fragments arranged by size:
4497 1346 578 6
ApaLI G'TGCA_C
Cuts at: 5197 5197
Size: 6427
ApoI r'AATT_y
Cuts at: 679 2951 3429 5780 6036 679
Size: 2272 478 2351 256 1070
Fragments arranged by size:
2351 2272 1070 478 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6427
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6427
AvaI C'yCGr_G
Cuts at: 1358 1849 3445 1358
Size: 491 1596 4340
Fragments arranged by size:
4340 1596 491
BamHI G'GATC_C
Cuts at: 3450 3450
Size: 6427
BanI G'GyrC_C
Cuts at: 708 1445 2714 2913 3207 3441 3855 4103 4138
Size: 737 1269 199 294 234 414 248 35
Cuts at: 4138 708
Size: 2997
Fragments arranged by size:
2997 1269 737 414 294 248 234 199
35
BanII G_rGCy'C
Cuts at: 730 1054 1061 1359 1365 1442 1676 1943 2051
Size: 324 7 298 6 77 234 267 108
Cuts at: 2051 2533 2638 3289 3439 4469 730
Size: 482 105 651 150 1030 2688
Fragments arranged by size:
2688 1030 651 482 324 298 267 234
150 108 105 77 7 6
BbeI G_GCGC'C
Cuts at: 2917 3859 4107 2917
Size: 942 248 5237
Fragments arranged by size:
5237 942 248
BbsI GAAGACnn'nnnn_
Cuts at: 1734 1734
Size: 6427
BbvCI CC'TCA_GC
Cuts at: 1046 1306 2507 1046
Size: 260 1201 4966
Fragments arranged by size:
4966 1201 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 816 850 1906 1940 816
Size: 34 1056 34 5303
Fragments arranged by size:
5303 1056 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3124 4323 5086 6427 3124
Size: 1199 763 1341 3124
Fragments arranged by size:
3124 1341 1199 763
BclI T'GATC_A
Cuts at: 3945 3945
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 1982 1982
Size: 6427
BglII A'GATC_T
Cuts at: 1716 2300 2873 3940 5979 1716
Size: 584 573 1067 2039 2164
Fragments arranged by size:
2164 2039 1067 584 573
BlpI GC'TnA_GC
Cuts at: 724 1495 1992 2407 2527 3141 3537 724
Size: 771 497 415 120 614 396 3614
Fragments arranged by size:
3614 771 614 497 415 396 120
Bme1580I G_kGCm'C
Cuts at: 1013 1359 1365 1943 3289 4050 4143 5201 1013
Size: 346 6 578 1346 761 93 1058 2239
Fragments arranged by size:
2239 1346 1058 761 578 346 93 6
BmgBI CAC'GTC
Cuts at: 3375 3375
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 1055 3637 3718 4053 6199 1055
Size: 2582 81 335 2146 1283
Fragments arranged by size:
2582 2146 1283 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4792 4792
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1195 1528 1546 1606 1893 2116 2299 3034
Size: 153 333 18 60 287 223 183 735
Cuts at: 3034 3145 3223 1042
Size: 111 78 4246
Fragments arranged by size:
4246 735 333 287 223 183 153 111
78 60 18
Bpu10I CC'TnA_GC
Cuts at: 1046 1306 2507 3118 1046
Size: 260 1201 611 4355
Fragments arranged by size:
4355 1201 611 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2452 3048 3060 3601 4480 4974 5272 2452
Size: 596 12 541 879 494 298 3607
Fragments arranged by size:
3607 879 596 541 494 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 1639 6131 1639
Size: 4492 1935
BsaAI yAC'GTr
Cuts at: 1731 2037 2073 3417 3778 1731
Size: 306 36 1344 361 4380
Fragments arranged by size:
4380 1344 361 306 36
BsaBI GATnn'nnATC
Cuts at: 6 3939 5978 6
Size: 3933 2039 455
Fragments arranged by size:
3933 2039 455
BsaHI Gr'CG_yC
Cuts at: 574 923 1531 2914 3856 4104 4808 6304 574
Size: 349 608 1383 942 248 704 1496 697
Fragments arranged by size:
1496 1383 942 704 697 608 349 248
BsaWI w'CCGG_w
Cuts at: 385 453 1648 1931 1946 3614 4135 4815 5089
Size: 68 1195 283 15 1668 521 680 274
Cuts at: 5089 5236 5647 385
Size: 147 411 1165
Fragments arranged by size:
1668 1195 1165 680 521 411 283 274
147 68 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 790 820 1756 1786 1897 1927 2071 2101 790
Size: 30 936 30 111 30 144 30 5116
Fragments arranged by size:
5116 936 144 111 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1342 1497 1780 2017 2020 2092 2776 2908 3256
Size: 155 283 237 3 72 684 132 348
Cuts at: 3256 3283 3378 1342
Size: 27 95 4391
Fragments arranged by size:
4391 684 348 283 237 155 132 95
72 27 3
BseYI C'CCAG_C
Cuts at: 184 252 499 1115 3850 4608 5187 5683 184
Size: 68 247 616 2735 758 579 496 928
Fragments arranged by size:
2735 928 758 616 579 496 247 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1498 1849 2458 2710 3257 3409 1120
Size: 378 351 609 252 547 152 4138
Fragments arranged by size:
4138 609 547 378 351 252 152
BsiEI CG_ry'CG
Cuts at: 104 299 3320 3967 4013 5223 104
Size: 195 3021 647 46 1210 1308
Fragments arranged by size:
3021 1308 1210 647 195 46
BsiHKAI G_wGCw'C
Cuts at: 730 1301 1442 1676 1730 2051 2359 2533 2744
Size: 571 141 234 54 321 308 174 211
Cuts at: 2744 3439 4217 4407 5201 730
Size: 695 778 190 794 1956
Fragments arranged by size:
1956 794 778 695 571 321 308 234
211 190 174 141 54
BsmI GAATG_Cn'
Cuts at: 1886 2386 1886
Size: 500 5927
BsmBI CGTCTCn'nnnn_
Cuts at: 1522 1522
Size: 6427
Bsp1286I G_dGCh'C
Cuts at: 730 1013 1054 1061 1301 1359 1365 1442 1676
Size: 283 41 7 240 58 6 77 234
Cuts at: 1676 1730 1943 2051 2359 2533 2638 2744 3289
Size: 54 213 108 308 174 105 106 545
Cuts at: 3289 3439 4050 4143 4217 4407 4469 5201 730
Size: 150 611 93 74 190 62 732 1956
Fragments arranged by size:
1956 732 611 545 308 283 240 234
213 190 174 150 108 106 105 93
77 74 62 58 54 41 7 6
BspEI T'CCGG_A
Cuts at: 453 1931 5647 453
Size: 1478 3716 1233
Fragments arranged by size:
3716 1478 1233
BspHI T'CATG_A
Cuts at: 476 5603 476
Size: 5127 1300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 208 292 620 1310 1571 2432 2522 3475 3689
Size: 84 328 690 261 861 90 953 214
Cuts at: 3689 3991 4372 208
Size: 302 381 2263
Fragments arranged by size:
2263 953 861 690 381 328 302 261
214 90 84
BsrBI CCG'CTC
Cuts at: 50 333 2333 3368 4717 4771 6257 6422 50
Size: 283 2000 1035 1349 54 1486 165 55
Fragments arranged by size:
2000 1486 1349 1035 283 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4337 4337
Size: 6427
BsrFI r'CCGG_y
Cuts at: 542 711 1648 1833 1976 2587 3039 4423 4604
Size: 169 937 185 143 611 452 1384 181
Cuts at: 4604 4815 542
Size: 211 2154
Fragments arranged by size:
2154 1384 937 611 452 211 185 181
169 143
BssHII G'CGCG_C
Cuts at: 248 2887 248
Size: 2639 3788
BssSI C'ACGA_G
Cuts at: 625 2728 3331 4696 5056 625
Size: 2103 603 1365 360 1996
Fragments arranged by size:
2103 1996 1365 603 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1185 2495 3802 1185
Size: 1310 1307 3810
Fragments arranged by size:
3810 1310 1307
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6427
BstXI CCAn_nnnn'nTGG
Cuts at: 3297 3297
Size: 6427
BstYI r'GATC_y
Cuts at: 1 1664 1716 2300 2873 3450 3917 3940 4275
Size: 1663 52 584 573 577 467 23 335
Cuts at: 4275 4521 5535 5621 5633 5979 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1663 1014 584 577 573 467 449 346
335 246 86 52 23 12
Bsu36I CC'TnA_GG
Cuts at: 1030 2224 3212 1030
Size: 1194 988 4245
Fragments arranged by size:
4245 1194 988
BtgI C'CryG_G
Cuts at: 165 193 288 1346 1986 2108 165
Size: 28 95 1058 640 122 4484
Fragments arranged by size:
4484 1058 640 122 95 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 573 654 2562 4375 4556 4719 573
Size: 81 1908 1813 181 163 2281
Fragments arranged by size:
2281 1908 1813 181 163 81
BtsI GCAGTG_nn'
Cuts at: 1186 2744 3668 1186
Size: 1558 924 3945
Fragments arranged by size:
3945 1558 924
ClaI AT'CG_AT
Cuts at: 755 755
Size: 6427
DraI TTT'AAA
Cuts at: 3424 3424
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 4131 4991 4131
Size: 860 5567
EaeI y'GGCC_r
Cuts at: 158 232 296 830 1316 1343 1808 2018 2690
Size: 74 64 534 486 27 465 210 672
Cuts at: 2690 4010 4184 4575 4602 5949 158
Size: 1320 174 391 27 1347 636
Fragments arranged by size:
1347 1320 672 636 534 486 465 391
210 174 74 64 27 27
EagI C'GGCC_G
Cuts at: 296 4010 296
Size: 3714 2713
EarI CTCTTCn'nnn_
Cuts at: 2749 4448 4658 2749
Size: 1699 210 4518
Fragments arranged by size:
4518 1699 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1411 3340 4633 4945 5091 6237 1411
Size: 1929 1293 312 146 1146 1601
Fragments arranged by size:
1929 1601 1293 1146 312 146
EcoICRI GAG'CTC
Cuts at: 728 1440 1674 2049 2531 3437 728
Size: 712 234 375 482 906 3718
Fragments arranged by size:
3718 906 712 482 375 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 1042 1195 1528 1546 1606 1816 1893 2116
Size: 616 153 333 18 60 210 77 223
Cuts at: 2116 2299 2449 2569 3034 3145 3223 4250 4682
Size: 183 150 120 465 111 78 1027 432
Cuts at: 4682 5431 5699 426
Size: 749 268 1154
Fragments arranged by size:
1154 1027 749 616 465 432 333 268
223 210 183 153 150 120 111 78
77 60 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1947 2518 1947
Size: 571 5856
EcoO109I rG'GnC_Cy
Cuts at: 777 788 1311 1355 1362 1939 1940 2511 3286
Size: 11 523 44 7 577 1 571 775
Cuts at: 3286 3343 3405 3564 777
Size: 57 62 159 3640
Fragments arranged by size:
3640 775 577 571 523 159 62 57
44 11 7 1
EcoRI G'AATT_C
Cuts at: 3429 3429
Size: 6427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 387 2829 4444 387
Size: 2442 1615 2370
Fragments arranged by size:
2442 2370 1615
FspI TGC'GCA
Cuts at: 586 953 2029 4206 586
Size: 367 1076 2177 2807
Fragments arranged by size:
2807 2177 1076 367
FspAI rTGC'GCAy
Cuts at: 953 953
Size: 6427
HaeII r_GCGC'y
Cuts at: 372 2527 2917 3013 3148 3859 4107 5131 6110
Size: 2155 390 96 135 711 248 1024 979
Cuts at: 6110 372
Size: 689
Fragments arranged by size:
2155 1024 979 711 689 390 248 135
96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 293 325 913 945 1228 1260 1318 1350 1447
Size: 32 588 32 283 32 58 32 97
Cuts at: 1447 1479 4267 4297 4299 4329 293
Size: 32 2788 30 2 30 2391
Fragments arranged by size:
2788 2391 588 283 97 58 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 932 2147 3464 932
Size: 1215 1317 3895
Fragments arranged by size:
3895 1317 1215
KasI G'GCGC_C
Cuts at: 2913 3855 4103 2913
Size: 942 248 5237
Fragments arranged by size:
5237 942 248
KpnI G_GTAC'C
Cuts at: 3445 3445
Size: 6427
MluI A'CGCG_T
Cuts at: 5860 5860
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 2244 2384 3160 3284 5098 5282 689
Size: 1555 140 776 124 1814 184 1834
Fragments arranged by size:
1834 1814 1555 776 184 140 124
MscI TGG'CCA
Cuts at: 1318 2020 2692 4186 1318
Size: 702 672 1494 3559
Fragments arranged by size:
3559 1494 702 672
MslI CAynn'nnrTG
Cuts at: 532 1451 2677 3319 4414 4541 532
Size: 919 1226 642 1095 127 2418
Fragments arranged by size:
2418 1226 1095 919 642 127
MspA1I CmG'CkG
Cuts at: 768 1119 1988 1996 2110 2460 2712 2976 3536
Size: 351 869 8 114 350 252 264 560
Cuts at: 3536 3850 4210 5225 5470 768
Size: 314 360 1015 245 1725
Fragments arranged by size:
1725 1015 869 560 360 351 350 314
264 252 245 114 8
NaeI GCC'GGC
Cuts at: 1978 3041 4606 1978
Size: 1063 1565 3799
Fragments arranged by size:
3799 1565 1063
NarI GG'CG_CC
Cuts at: 2914 3856 4104 2914
Size: 942 248 5237
Fragments arranged by size:
5237 942 248
NgoMIV G'CCGG_C
Cuts at: 1976 3039 4604 1976
Size: 1063 1565 3799
Fragments arranged by size:
3799 1565 1063
NruI TCG'CGA
Cuts at: 4788 4788
Size: 6427
NspI r_CATG'y
Cuts at: 231 1294 3478 3978 4887 231
Size: 1063 2184 500 909 1771
Fragments arranged by size:
2184 1771 1063 909 500
PciI A'CATG_T
Cuts at: 4883 4883
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 1541 3201 1541
Size: 1660 4767
PfoI T'CCnGG_A
Cuts at: 350 1019 1214 1622 1667 2051 2093 2375 350
Size: 669 195 408 45 384 42 282 4402
Fragments arranged by size:
4402 669 408 384 282 195 45 42
PmeI GTTT'AAAC
Cuts at: 3424 3424
Size: 6427
PmlI CAC'GTG
Cuts at: 1731 2037 1731
Size: 306 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1756 1788 1897 1929 5566 5598 1756
Size: 32 109 32 3637 32 2585
Fragments arranged by size:
3637 2585 109 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 777 788 1311 2511 777
Size: 11 523 1200 4693
Fragments arranged by size:
4693 1200 523 11
PspOMI G'GGCC_C
Cuts at: 1355 1361 1939 3285 1355
Size: 6 578 1346 4497
Fragments arranged by size:
4497 1346 578 6
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1387 1419 1387
Size: 32 6395
PstI C_TGCA'G
Cuts at: 274 509 617 881 1043 1190 2519 2981 3472
Size: 235 108 264 162 147 1329 462 491
Cuts at: 3472 4157 274
Size: 685 2544
Fragments arranged by size:
2544 1329 685 491 462 264 235 162
147 108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6427
PvuII CAG'CTG
Cuts at: 768 1119 2460 2976 3850 4210 768
Size: 351 1341 516 874 360 2985
Fragments arranged by size:
2985 1341 874 516 360 351
RsrII CG'GwC_CG
Cuts at: 4620 4620
Size: 6427
SacI G_AGCT'C
Cuts at: 730 1442 1676 2051 2533 3439 730
Size: 712 234 375 482 906 3718
Fragments arranged by size:
3718 906 712 482 375 234
SacII CC_GC'GG
Cuts at: 1989 2111 1989
Size: 122 6305
SalI G'TCGA_C
Cuts at: 930 3462 930
Size: 2532 3895
SanDI GG'GwC_CC
Cuts at: 788 788
Size: 6427
SapI GCTCTTCn'nnn_
Cuts at: 2749 4448 4658 2749
Size: 1699 210 4518
Fragments arranged by size:
4518 1699 210
SbfI CC_TGCA'GG
Cuts at: 509 2519 3472 509
Size: 2010 953 3464
Fragments arranged by size:
3464 2010 953
ScaI AGT'ACT
Cuts at: 2314 5944 2314
Size: 3630 2797
SfcI C'TryA_G
Cuts at: 33 270 505 613 877 1039 1186 2515 2796
Size: 237 235 108 264 162 147 1329 281
Cuts at: 2796 2977 3468 4153 5148 5339 33
Size: 181 491 685 995 191 1121
Fragments arranged by size:
1329 1121 995 685 491 281 264 237
235 191 181 162 147 108
SfoI GGC'GCC
Cuts at: 2915 3857 4105 2915
Size: 942 248 5237
Fragments arranged by size:
5237 942 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6427
SmaI CCC'GGG
Cuts at: 1360 3447 1360
Size: 2087 4340
SmlI C'TyrA_G
Cuts at: 2467 3063 3075 3580 4495 4989 5251 2467
Size: 596 12 505 915 494 262 3643
Fragments arranged by size:
3643 915 596 505 494 262 12
SnaBI TAC'GTA
Cuts at: 3417 3417
Size: 6427
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6427
SphI G_CATG'C
Cuts at: 231 3478 3978 231
Size: 3247 500 2680
Fragments arranged by size:
3247 2680 500
SrfI GCCC'GGGC
Cuts at: 1360 1360
Size: 6427
StuI AGG'CCT
Cuts at: 2153 2153
Size: 6427
StyI C'CwwG_G
Cuts at: 2675 3559 3913 2675
Size: 884 354 5189
Fragments arranged by size:
5189 884 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 772 787 907 2668 2931 4008 4141 4600 4811
Size: 15 120 1761 263 1077 133 459 211
Cuts at: 4811 772
Size: 2388
Fragments arranged by size:
2388 1761 1077 459 263 211 133 120
15
TatI w'GTAC_w
Cuts at: 2312 5942 2312
Size: 3630 2797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2071 2103 5944 5976 2071
Size: 32 3841 32 2522
Fragments arranged by size:
3841 2522 32 32
Tth111I GACn'n_nGTC
Cuts at: 325 4222 325
Size: 3897 2530
XbaI T'CTAG_A
Cuts at: 3456 3456
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3920 3920
Size: 6427
XmaI C'CCGG_G
Cuts at: 1358 3445 1358
Size: 2087 4340
ZraI GAC'GTC
Cuts at: 924 1532 924
Size: 608 5819
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AflII AlfI AscI AvrII BaeI BfrBI BmtI BsiWI
BsrGI BstEII BstZ17I CspCI DraIII EcoRV FseI HindIII
HpaI MfeI NcoI NdeI NheI NotI NsiI PacI
PasI PshAI PsiI PspXI SexAI SfiI SgrAI SspI
SwaI XhoI XmnI