Restriction map for
pF1KSDA0331
(Circular) (Six-base) MAPSORT of: pF1KSDA0331.seq Check: 5397 from: 1 to: 5462
pF1KSDA0331 5462 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AatII G_ACGT'C
Cuts at: 1475 1475
Size: 5462
AccI GT'mk_AC
Cuts at: 2500 2500
Size: 5462
Acc65I G'GTAC_C
Cuts at: 2478 2478
Size: 5462
AclI AA'CG_TT
Cuts at: 5302 5302
Size: 5462
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 1901 3287 3719 4466 4734 1555
Size: 346 1386 432 747 268 2283
Fragments arranged by size:
2283 1386 747 432 346 268
AflIII A'CryG_T
Cuts at: 1160 1685 2814 3918 4895 1160
Size: 525 1129 1104 977 1727
Fragments arranged by size:
1727 1129 1104 977 525
AgeI A'CCGG_T
Cuts at: 3850 3850
Size: 5462
AhdI GACnn_n'nnGTC
Cuts at: 191 191
Size: 5462
AlwNI CAG_nnn'CTG
Cuts at: 2071 2767 4334 2071
Size: 696 1567 3199
Fragments arranged by size:
3199 1567 696
ApaLI G'TGCA_C
Cuts at: 2325 4232 2325
Size: 1907 3555
ApoI r'AATT_y
Cuts at: 802 2260 2466 4815 5071 802
Size: 1458 206 2349 256 1193
Fragments arranged by size:
2349 1458 1193 256 206
AseI AT'TA_AT
Cuts at: 20 20
Size: 5462
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
AvaI C'yCGr_G
Cuts at: 1721 2482 1721
Size: 761 4701
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1342 1375 1342
Size: 33 5429
BamHI G'GATC_C
Cuts at: 582 1584 2487 582
Size: 1002 903 3557
Fragments arranged by size:
3557 1002 903
BanI G'GyrC_C
Cuts at: 2478 2892 3140 3175 2478
Size: 414 248 35 4765
Fragments arranged by size:
4765 414 248 35
BanII G_rGCy'C
Cuts at: 245 2476 3506 245
Size: 2231 1030 2201
Fragments arranged by size:
2231 2201 1030
BbeI G_GCGC'C
Cuts at: 2896 3144 2896
Size: 248 5214
BbsI GAAGACnn'nnnn_
Cuts at: 842 2011 842
Size: 1169 4293
BbvCI CC'TCA_GC
Cuts at: 367 1454 367
Size: 1087 4375
BciVI GTATCCnnnnn_n'
Cuts at: 3360 4121 5462 3360
Size: 761 1341 3360
Fragments arranged by size:
3360 1341 761
BclI T'GATC_A
Cuts at: 2982 2982
Size: 5462
BfrBI ATG'CAT
Cuts at: 440 440
Size: 5462
BglII A'GATC_T
Cuts at: 610 2977 5014 610
Size: 2367 2037 1058
Fragments arranged by size:
2367 2037 1058
BlpI GC'TnA_GC
Cuts at: 1832 2574 1832
Size: 742 4720
Bme1580I G_kGCm'C
Cuts at: 143 2329 3087 3180 4236 143
Size: 2186 758 93 1056 1369
Fragments arranged by size:
2186 1369 1056 758 93
BmgBI CAC'GTC
Cuts at: 1981 1981
Size: 5462
BmrI ACTGGGnnnn_n'
Cuts at: 2674 2755 3090 5234 2674
Size: 81 335 2144 2902
Fragments arranged by size:
2902 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 437 3829 437
Size: 3392 2070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 551 732 904 196
Size: 355 181 172 4754
Fragments arranged by size:
4754 355 181 172
Bpu10I CC'TnA_GC
Cuts at: 367 1454 2414 367
Size: 1087 960 3415
Fragments arranged by size:
3415 1087 960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1646 2638 3517 4009 4307 1646
Size: 992 879 492 298 2801
Fragments arranged by size:
2801 992 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1718 5166 1718
Size: 3448 2014
BsaAI yAC'GTr
Cuts at: 2454 2815 2454
Size: 361 5101
BsaBI GATnn'nnATC
Cuts at: 6 320 2976 5013 6
Size: 314 2656 2037 455
Fragments arranged by size:
2656 2037 455 314
BsaHI Gr'CG_yC
Cuts at: 1472 2893 3141 3843 5339 1472
Size: 1421 248 702 1496 1595
Fragments arranged by size:
1595 1496 1421 702 248
BsaWI w'CCGG_w
Cuts at: 1799 2651 3172 3850 4124 4271 4682 1799
Size: 852 521 678 274 147 411 2579
Fragments arranged by size:
2579 852 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 634 664 634
Size: 30 5432
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 642 2011 2164 2200 642
Size: 1369 153 36 3904
Fragments arranged by size:
3904 1369 153 36
BseYI C'CCAG_C
Cuts at: 646 2071 2887 3645 4222 4718 646
Size: 1425 816 758 577 496 1390
Fragments arranged by size:
1425 1390 816 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3004 3050 4258 104
Size: 2900 46 1208 1308
Fragments arranged by size:
2900 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 245 982 2329 2476 3254 3444 4236 245
Size: 737 1347 147 778 190 792 1471
Fragments arranged by size:
1471 1347 792 778 737 190 147
BsmI GAATG_Cn'
Cuts at: 442 1169 442
Size: 727 4735
BsmBI CGTCTCn'nnnn_
Cuts at: 713 713
Size: 5462
Bsp1286I G_dGCh'C
Cuts at: 143 245 982 2329 2476 3087 3180 3254 3444
Size: 102 737 1347 147 611 93 74 190
Cuts at: 3444 3506 4236 143
Size: 62 730 1369
Fragments arranged by size:
1369 1347 737 730 611 190 147 102
93 74 62
BspEI T'CCGG_A
Cuts at: 1799 4682 1799
Size: 2883 2579
BspHI T'CATG_A
Cuts at: 4638 4638
Size: 5462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1383 2512 2726 3028 3409 1383
Size: 1129 214 302 381 3436
Fragments arranged by size:
3436 1129 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3754 3808 5292 5457 50
Size: 3704 54 1484 165 55
Fragments arranged by size:
3704 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3374 3374
Size: 5462
BsrFI r'CCGG_y
Cuts at: 3460 3641 3850 3460
Size: 181 209 5072
Fragments arranged by size:
5072 209 181
BsrGI T'GTAC_A
Cuts at: 1968 1968
Size: 5462
BssSI C'ACGA_G
Cuts at: 3733 4091 3733
Size: 358 5104
BstAPI GCAn_nnn'nTGC
Cuts at: 1754 2839 1754
Size: 1085 4377
BstBI TT'CG_AA
Cuts at: 110 3823 110
Size: 3713 1749
BstYI r'GATC_y
Cuts at: 1 582 610 1584 1642 2487 2954 2977 3312
Size: 581 28 974 58 845 467 23 335
Cuts at: 3312 3558 4570 4656 4668 5014 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 974 845 581 467 449 346 335
246 86 58 28 23 12
Bsu36I CC'TnA_GG
Cuts at: 2050 2387 2050
Size: 337 5125
BtgI C'CryG_G
Cuts at: 125 134 2248 125
Size: 9 2114 3339
Fragments arranged by size:
3339 2114 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3412 3593 3756 3412
Size: 181 163 5118
Fragments arranged by size:
5118 181 163
BtsI GCAGTG_nn'
Cuts at: 1843 2705 1843
Size: 862 4600
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 654 689 654
Size: 35 5427
DraI TTT'AAA
Cuts at: 2461 2461
Size: 5462
DrdI GACnn_nn'nnGTC
Cuts at: 3168 4026 3168
Size: 858 4604
EaeI y'GGCC_r
Cuts at: 409 1461 3047 3221 3612 3639 4984 409
Size: 1052 1586 174 391 27 1345 887
Fragments arranged by size:
1586 1345 1052 887 391 174 27
EagI C'GGCC_G
Cuts at: 3047 3047
Size: 5462
EarI CTCTTCn'nnn_
Cuts at: 340 2050 2141 3485 3695 340
Size: 1710 91 1344 210 2107
Fragments arranged by size:
2107 1710 1344 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3670 3980 4126 5272 3670
Size: 310 146 1146 3860
Fragments arranged by size:
3860 1146 310 146
EcoICRI GAG'CTC
Cuts at: 243 2474 243
Size: 2231 3231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 551 732 904 1555 1901 3287 3719 4466
Size: 355 181 172 651 346 1386 432 747
Cuts at: 4466 4734 196
Size: 268 924
Fragments arranged by size:
1386 924 747 651 432 355 346 268
181 172
EcoO109I rG'GnC_Cy
Cuts at: 351 1210 2075 2601 351
Size: 859 865 526 3212
Fragments arranged by size:
3212 865 859 526
EcoRI G'AATT_C
Cuts at: 2260 2466 2260
Size: 206 5256
EcoRV GAT'ATC
Cuts at: 571 571
Size: 5462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2280 3481 2280
Size: 1201 4261
FspI TGC'GCA
Cuts at: 3243 3243
Size: 5462
HaeII r_GCGC'y
Cuts at: 2896 3144 4166 5145 2896
Size: 248 1022 979 3213
Fragments arranged by size:
3213 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 177 209 634 666 1735 1767 2196 2228 3304
Size: 32 425 32 1069 32 429 32 1076
Cuts at: 3304 3334 3336 3366 177
Size: 30 2 30 2273
Fragments arranged by size:
2273 1076 1069 429 425 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2501 2501
Size: 5462
HindIII A'AGCT_T
Cuts at: 2347 2347
Size: 5462
KasI G'GCGC_C
Cuts at: 2892 3140 2892
Size: 248 5214
KpnI G_GTAC'C
Cuts at: 2482 2482
Size: 5462
MluI A'CGCG_T
Cuts at: 4895 4895
Size: 5462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 546 4133 4317 546
Size: 3587 184 1691
Fragments arranged by size:
3587 1691 184
MscI TGG'CCA
Cuts at: 1463 3223 1463
Size: 1760 3702
MslI CAynn'nnrTG
Cuts at: 776 1679 1823 3451 3578 776
Size: 903 144 1628 127 2660
Fragments arranged by size:
2660 1628 903 144 127
MspA1I CmG'CkG
Cuts at: 136 202 1632 2271 2573 2887 3247 4260 4505
Size: 66 1430 639 302 314 360 1013 245
Cuts at: 4505 136
Size: 1093
Fragments arranged by size:
1430 1093 1013 639 360 314 302 245
66
NaeI GCC'GGC
Cuts at: 3643 3643
Size: 5462
NarI GG'CG_CC
Cuts at: 2893 3141 2893
Size: 248 5214
NcoI C'CATG_G
Cuts at: 125 2248 125
Size: 2123 3339
NdeI CA'TA_TG
Cuts at: 1194 1194
Size: 5462
NgoMIV G'CCGG_C
Cuts at: 3641 3641
Size: 5462
NsiI A_TGCA'T
Cuts at: 442 442
Size: 5462
NspI r_CATG'y
Cuts at: 1144 1164 1689 1786 2515 3015 3922 1144
Size: 20 525 97 729 500 907 2684
Fragments arranged by size:
2684 907 729 525 500 97 20
PciI A'CATG_T
Cuts at: 1160 1685 3918 1160
Size: 525 2233 2704
Fragments arranged by size:
2704 2233 525
PflMI CCAn_nnn'nTGG
Cuts at: 1261 1536 2298 1261
Size: 275 762 4425
Fragments arranged by size:
4425 762 275
PmeI GTTT'AAAC
Cuts at: 2461 2461
Size: 5462
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 4601
Size: 32 5430
PpuMI rG'GwC_Cy
Cuts at: 351 1210 2075 351
Size: 859 865 3738
Fragments arranged by size:
3738 865 859
PstI C_TGCA'G
Cuts at: 2509 3194 2509
Size: 685 4777
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5462
PvuII CAG'CTG
Cuts at: 202 1632 2271 2887 3247 202
Size: 1430 639 616 360 2417
Fragments arranged by size:
2417 1430 639 616 360
RsrII CG'GwC_CG
Cuts at: 2118 3657 2118
Size: 1539 3923
SacI G_AGCT'C
Cuts at: 245 2476 245
Size: 2231 3231
SacII CC_GC'GG
Cuts at: 137 137
Size: 5462
SalI G'TCGA_C
Cuts at: 2499 2499
Size: 5462
SapI GCTCTTCn'nnn_
Cuts at: 340 3485 3695 340
Size: 3145 210 2107
Fragments arranged by size:
3145 2107 210
SbfI CC_TGCA'GG
Cuts at: 2509 2509
Size: 5462
ScaI AGT'ACT
Cuts at: 1917 4979 1917
Size: 3062 2400
SfcI C'TryA_G
Cuts at: 33 699 2505 3190 4183 4374 33
Size: 666 1806 685 993 191 1121
Fragments arranged by size:
1806 1121 993 685 666 191
SfoI GGC'GCC
Cuts at: 2894 3142 2894
Size: 248 5214
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
SmaI CCC'GGG
Cuts at: 2484 2484
Size: 5462
SmlI C'TyrA_G
Cuts at: 1661 1721 2617 3532 4024 4286 1661
Size: 60 896 915 492 262 2837
Fragments arranged by size:
2837 915 896 492 262 60
SnaBI TAC'GTA
Cuts at: 2454 2454
Size: 5462
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5462
SphI G_CATG'C
Cuts at: 1144 2515 3015 1144
Size: 1371 500 3591
Fragments arranged by size:
3591 1371 500
SspI AAT'ATT
Cuts at: 269 1128 1394 269
Size: 859 266 4337
Fragments arranged by size:
4337 859 266
StuI AGG'CCT
Cuts at: 1260 1260
Size: 5462
StyI C'CwwG_G
Cuts at: 125 1255 1307 2034 2132 2248 2596 2950 125
Size: 1130 52 727 98 116 348 354 2637
Fragments arranged by size:
2637 1130 727 354 348 116 98 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 621 3045 3178 3637 3846 621
Size: 2424 133 459 209 2237
Fragments arranged by size:
2424 2237 459 209 133
TatI w'GTAC_w
Cuts at: 415 1915 1968 4977 415
Size: 1500 53 3009 900
Fragments arranged by size:
3009 1500 900 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4979 5011 4979
Size: 32 5430
Tth111I GACn'n_nGTC
Cuts at: 3259 3259
Size: 5462
XbaI T'CTAG_A
Cuts at: 1560 2493 1560
Size: 933 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2439 2957 2439
Size: 518 4944
XhoI C'TCGA_G
Cuts at: 1721 1721
Size: 5462
XmaI C'CCGG_G
Cuts at: 2482 2482
Size: 5462
XmnI GAAnn'nnTTC
Cuts at: 1556 1568 1556
Size: 12 5450
ZraI GAC'GTC
Cuts at: 1473 1473
Size: 5462
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BcgI BglI BmtI BsgI BsiWI BssHII BstEII
BstXI BstZ17I ClaI DraIII EcoNI FseI FspAI HpaI
MfeI NheI NotI NruI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SrfI SwaI