Restriction map for
pF1KSDA0216
(Circular) (Six-base) MAPSORT of: pF1KSDA0216.seq Check: 258 from: 1 to: 9299
pF1KSDA0216 9299 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4216 5285 4216
Size: 1069 8230
AccI GT'mk_AC
Cuts at: 734 2489 6337 734
Size: 1755 3848 3696
Fragments arranged by size:
3848 3696 1755
Acc65I G'GTAC_C
Cuts at: 6315 6315
Size: 9299
AclI AA'CG_TT
Cuts at: 9139 9139
Size: 9299
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 4153 4264 4639 5119 5170 5320 7124 7556
Size: 1961 111 375 480 51 150 1804 432
Cuts at: 7556 8303 8571 2192
Size: 747 268 2920
Fragments arranged by size:
2920 1961 1804 747 480 432 375 268
150 111 51
AfeI AGC'GCT
Cuts at: 538 1408 2299 5312 538
Size: 870 891 3013 4525
Fragments arranged by size:
4525 3013 891 870
AflIII A'CryG_T
Cuts at: 1430 1505 6651 7755 8732 1430
Size: 75 5146 1104 977 1997
Fragments arranged by size:
5146 1997 1104 977 75
AgeI A'CCGG_T
Cuts at: 7687 7687
Size: 9299
AhdI GACnn_n'nnGTC
Cuts at: 5070 5070
Size: 9299
AleI CACnn'nnGTG
Cuts at: 2282 2373 2608 2282
Size: 91 235 8973
Fragments arranged by size:
8973 235 91
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 859 1451 2391 2626 5432 859
Size: 592 940 235 2806 4726
Fragments arranged by size:
4726 2806 940 592 235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 262 294 5197 5229 262
Size: 32 4903 32 4332
Fragments arranged by size:
4903 4332 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1082 1656 1668 1728 2721 3614 3771 3921 4059
Size: 574 12 60 993 893 157 150 138
Cuts at: 4059 4428 4827 5502 5877 6118 6158 6604 8171
Size: 369 399 675 375 241 40 446 1567
Cuts at: 8171 1082
Size: 2210
Fragments arranged by size:
2210 1567 993 893 675 574 446 399
375 369 241 157 150 138 60 40
12
ApaI G_GGCC'C
Cuts at: 859 1476 2211 2323 859
Size: 617 735 112 7835
Fragments arranged by size:
7835 735 617 112
ApaLI G'TGCA_C
Cuts at: 2366 3256 8069 2366
Size: 890 4813 3596
Fragments arranged by size:
4813 3596 890
ApoI r'AATT_y
Cuts at: 6303 8652 8908 6303
Size: 2349 256 6694
Fragments arranged by size:
6694 2349 256
AseI AT'TA_AT
Cuts at: 20 962 1772 20
Size: 942 810 7547
Fragments arranged by size:
7547 942 810
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9299
AvaI C'yCGr_G
Cuts at: 405 698 859 2323 2496 3351 3934 4357 5435
Size: 293 161 1464 173 855 583 423 1078
Cuts at: 5435 6319 405
Size: 884 3385
Fragments arranged by size:
3385 1464 1078 884 855 583 423 293
173 161
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1351 1384 1351
Size: 33 9266
BamHI G'GATC_C
Cuts at: 6324 6324
Size: 9299
BanI G'GyrC_C
Cuts at: 689 756 910 936 1536 1886 1945 2287 2790
Size: 67 154 26 600 350 59 342 503
Cuts at: 2790 2926 3649 6315 6729 6977 7012 689
Size: 136 723 2666 414 248 35 2976
Fragments arranged by size:
2976 2666 723 600 503 414 350 342
248 154 136 67 59 35 26
BanII G_rGCy'C
Cuts at: 705 859 1064 1073 1238 1476 1964 2211 2323
Size: 154 205 9 165 238 488 247 112
Cuts at: 2323 2414 2448 3299 3377 4398 4526 4694 6313
Size: 91 34 851 78 1021 128 168 1619
Cuts at: 6313 7343 705
Size: 1030 2661
Fragments arranged by size:
2661 1619 1030 1021 851 488 247 238
205 168 165 154 128 112 91 78
34 9
BbeI G_GCGC'C
Cuts at: 1890 6733 6981 1890
Size: 4843 248 4208
Fragments arranged by size:
4843 4208 248
BbsI GAAGACnn'nnnn_
Cuts at: 1908 5273 5390 1908
Size: 3365 117 5817
Fragments arranged by size:
5817 3365 117
BbvCI CC'TCA_GC
Cuts at: 1839 2256 3278 4604 5041 1839
Size: 417 1022 1326 437 6097
Fragments arranged by size:
6097 1326 1022 437 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2742 2776 2742
Size: 34 9265
BciVI GTATCCnnnnn_n'
Cuts at: 7197 7958 9299 7197
Size: 761 1341 7197
Fragments arranged by size:
7197 1341 761
BclI T'GATC_A
Cuts at: 6819 6819
Size: 9299
BglI GCCn_nnn'nGGC
Cuts at: 863 2215 2384 3059 3101 5119 863
Size: 1352 169 675 42 2018 5043
Fragments arranged by size:
5043 2018 1352 675 169 42
BglII A'GATC_T
Cuts at: 6814 8851 6814
Size: 2037 7262
BlpI GC'TnA_GC
Cuts at: 896 1063 1072 2042 4436 5326 5468 6411 896
Size: 167 9 970 2394 890 142 943 3784
Fragments arranged by size:
3784 2394 970 943 890 167 142 9
Bme1580I G_kGCm'C
Cuts at: 692 761 859 971 1476 2211 2323 2370 3260
Size: 69 98 112 505 735 112 47 890
Cuts at: 3260 3395 3652 6924 7017 8073 692
Size: 135 257 3272 93 1056 1918
Fragments arranged by size:
3272 1918 1056 890 735 505 257 135
112 112 98 93 69 47
BmgBI CAC'GTC
Cuts at: 253 3390 253
Size: 3137 6162
BmrI ACTGGGnnnn_n'
Cuts at: 724 2239 2943 4113 6511 6592 6927 9071 724
Size: 1515 704 1170 2398 81 335 2144 952
Fragments arranged by size:
2398 2144 1515 1170 952 704 335 81
BmtI G_CTAG'C
Cuts at: 1421 5663 1421
Size: 4242 5057
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 591 2271 3123 4065 6048 7666 591
Size: 1680 852 942 1983 1618 2224
Fragments arranged by size:
2224 1983 1680 1618 942 852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 414 808 830 1366 1885 2263 2392 2839
Size: 161 394 22 536 519 378 129 447
Cuts at: 2839 3118 3604 3619 3685 4006 4276 4336 4483
Size: 279 486 15 66 321 270 60 147
Cuts at: 4483 4513 4678 4786 4870 5041 5221 5335 5389
Size: 30 165 108 84 171 180 114 54
Cuts at: 5389 5509 5566 5626 5638 5668 5857 5878 6073
Size: 120 57 60 12 30 189 21 195
Cuts at: 6073 6201 253
Size: 128 3351
Fragments arranged by size:
3351 536 519 486 447 394 378 321
279 270 195 189 180 171 165 161
147 129 128 120 114 108 84 66
60 60 57 54 30 30 22 21
15 12
Bpu10I CC'TnA_GC
Cuts at: 707 1839 2256 2513 3278 3625 4604 5041 5426
Size: 1132 417 257 765 347 979 437 385
Cuts at: 5426 707
Size: 4580
Fragments arranged by size:
4580 1132 979 765 437 417 385 347
257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1024 2401 2434 4393 5173 5653 6043 6475
Size: 597 1377 33 1959 780 480 390 432
Cuts at: 6475 7354 7846 8144 427
Size: 879 492 298 1582
Fragments arranged by size:
1959 1582 1377 879 780 597 492 480
432 390 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 3350 9003 3350
Size: 5653 3646
BsaAI yAC'GTr
Cuts at: 1433 6291 6652 1433
Size: 4858 361 4080
Fragments arranged by size:
4858 4080 361
BsaBI GATnn'nnATC
Cuts at: 6 6813 8850 6
Size: 6807 2037 455
Fragments arranged by size:
6807 2037 455
BsaHI Gr'CG_yC
Cuts at: 589 1887 3215 6730 6978 7680 9176 589
Size: 1298 1328 3515 248 702 1496 712
Fragments arranged by size:
3515 1496 1328 1298 712 702 248
BsaWI w'CCGG_w
Cuts at: 3893 6488 7009 7687 7961 8108 8519 3893
Size: 2595 521 678 274 147 411 4673
Fragments arranged by size:
4673 2595 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 262 292 355 385 761 791 970
Size: 30 39 30 63 30 376 30 179
Cuts at: 970 1000 1165 1195 2236 2266 2692 2722 5197
Size: 30 165 30 1041 30 426 30 2475
Cuts at: 5197 5227 5507 5537 6199 6229 193
Size: 30 280 30 662 30 3263
Fragments arranged by size:
3263 2475 1041 662 426 376 280 179
165 63 39 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 267 466 1246 2260 2341 2563 2650 2794
Size: 17 199 780 1014 81 222 87 144
Cuts at: 2794 3300 3589 3682 3898 3922 4396 4521 4555
Size: 506 289 93 216 24 474 125 34
Cuts at: 4555 4588 4924 4933 5218 5332 5710 5794 250
Size: 33 336 9 285 114 378 84 3755
Fragments arranged by size:
3755 1014 780 506 474 378 336 289
285 222 216 199 144 125 114 93
87 84 81 34 33 24 17 9
BseYI C'CCAG_C
Cuts at: 551 1444 1890 2079 2176 2982 3284 3289 3370
Size: 893 446 189 97 806 302 5 81
Cuts at: 3370 3407 4052 4547 5129 5164 5183 6724 7482
Size: 37 645 495 582 35 19 1541 758
Cuts at: 7482 8059 8555 551
Size: 577 496 1295
Fragments arranged by size:
1541 1295 893 806 758 645 582 577
496 495 446 302 189 97 81 37
35 19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 676 926 1066 1375 1411 1681 2632 2866
Size: 339 250 140 309 36 270 951 234
Cuts at: 2866 4441 4984 5257 337
Size: 1575 543 273 4379
Fragments arranged by size:
4379 1575 951 543 339 309 273 270
250 234 140 36
BsiEI CG_ry'CG
Cuts at: 104 1341 2396 6841 6887 8095 104
Size: 1237 1055 4445 46 1208 1308
Fragments arranged by size:
4445 1308 1237 1208 1055 46
BsiHKAI G_wGCw'C
Cuts at: 602 1064 1073 1238 1964 2370 2414 3077 3260
Size: 462 9 165 726 406 44 663 183
Cuts at: 3260 4526 4694 5163 6197 6313 7091 7281 8073
Size: 1266 168 469 1034 116 778 190 792
Cuts at: 8073 602
Size: 1828
Fragments arranged by size:
1828 1266 1034 792 778 726 663 469
462 406 190 183 168 165 116 44
9
BsmI GAATG_Cn'
Cuts at: 3000 5899 3000
Size: 2899 6400
BsmBI CGTCTCn'nnnn_
Cuts at: 1162 2433 4183 5671 1162
Size: 1271 1750 1488 4790
Fragments arranged by size:
4790 1750 1488 1271
Bsp1286I G_dGCh'C
Cuts at: 602 692 705 761 859 971 1064 1073 1238
Size: 90 13 56 98 112 93 9 165
Cuts at: 1238 1476 1964 2211 2323 2370 2414 2448 3077
Size: 238 488 247 112 47 44 34 629
Cuts at: 3077 3260 3299 3377 3395 3652 4398 4526 4694
Size: 183 39 78 18 257 746 128 168
Cuts at: 4694 5163 6197 6313 6924 7017 7091 7281 7343
Size: 469 1034 116 611 93 74 190 62
Cuts at: 7343 8073 602
Size: 730 1828
Fragments arranged by size:
1828 1034 746 730 629 611 488 469
257 247 238 190 183 168 165 128
116 112 112 98 93 93 90 78
74 62 56 47 44 39 34 18
13 9
BspEI T'CCGG_A
Cuts at: 3893 8519 3893
Size: 4626 4673
BspHI T'CATG_A
Cuts at: 8475 8475
Size: 9299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 359 746 1934 3353 4216 4373 4464 5279 5285
Size: 387 1188 1419 863 157 91 815 6
Cuts at: 5285 5927 6349 6563 6865 7246 359
Size: 642 422 214 302 381 2412
Fragments arranged by size:
2412 1419 1188 863 815 642 422 387
381 302 214 157 91 6
BsrBI CCG'CTC
Cuts at: 50 204 275 1094 2729 4254 4866 5700 7591
Size: 154 71 819 1635 1525 612 834 1891
Cuts at: 7591 7645 9129 9294 50
Size: 54 1484 165 55
Fragments arranged by size:
1891 1635 1525 1484 834 819 612 165
154 71 55 54
BsrDI GCAATG_nn'
Cuts at: 1785 4649 5255 5711 7211 1785
Size: 2864 606 456 1500 3873
Fragments arranged by size:
3873 2864 1500 606 456
BsrFI r'CCGG_y
Cuts at: 2395 2576 3980 5057 5363 7297 7478 7687 2395
Size: 181 1404 1077 306 1934 181 209 4007
Fragments arranged by size:
4007 1934 1404 1077 306 209 181 181
BsrGI T'GTAC_A
Cuts at: 1730 3786 1730
Size: 2056 7243
BssSI C'ACGA_G
Cuts at: 2596 7570 7928 2596
Size: 4974 358 3967
Fragments arranged by size:
4974 3967 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1398 1659 3064 3255 3614 5502 6676 1398
Size: 261 1405 191 359 1888 1174 4021
Fragments arranged by size:
4021 1888 1405 1174 359 261 191
BstBI TT'CG_AA
Cuts at: 110 7660 110
Size: 7550 1749
BstEII G'GTnAC_C
Cuts at: 3457 3457
Size: 9299
BstXI CCAn_nnnn'nTGG
Cuts at: 333 921 1411 1452 2923 3162 333
Size: 588 490 41 1471 239 6470
Fragments arranged by size:
6470 1471 588 490 239 41
BstYI r'GATC_y
Cuts at: 1 1350 1799 3594 3761 3890 3911 3995 5354
Size: 1349 449 1795 167 129 21 84 1359
Cuts at: 5354 5844 6324 6791 6814 7149 7395 8407 8493
Size: 490 480 467 23 335 246 1012 86
Cuts at: 8493 8505 8851 1
Size: 12 346 449
Fragments arranged by size:
1795 1359 1349 1012 490 480 467 449
449 346 335 246 167 129 86 84
23 21 12
Bsu36I CC'TnA_GG
Cuts at: 1247 1951 2021 1247
Size: 704 70 8525
Fragments arranged by size:
8525 704 70
BtgI C'CryG_G
Cuts at: 465 752 1467 2532 2922 3068 3414 5420 5555
Size: 287 715 1065 390 146 346 2006 135
Cuts at: 5555 465
Size: 4209
Fragments arranged by size:
4209 2006 1065 715 390 346 287 146
135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 449 1591 1662 3072 5277 7249 7430 7593 449
Size: 1142 71 1410 2205 1972 181 163 2155
Fragments arranged by size:
2205 2155 1972 1410 1142 181 163 71
BtsI GCAGTG_nn'
Cuts at: 1559 1635 3069 3118 3258 3339 3360 3575 6542
Size: 76 1434 49 140 81 21 215 2967
Cuts at: 6542 1559
Size: 4316
Fragments arranged by size:
4316 2967 1434 215 140 81 76 49
21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5237 5272 5237
Size: 35 9264
DraI TTT'AAA
Cuts at: 6298 6298
Size: 9299
DraIII CAC_nnn'GTG
Cuts at: 975 3474 975
Size: 2499 6800
DrdI GACnn_nn'nnGTC
Cuts at: 7005 7863 7005
Size: 858 8441
EaeI y'GGCC_r
Cuts at: 335 506 1211 1670 1827 2012 2066 2393 3773
Size: 171 705 459 157 185 54 327 1380
Cuts at: 3773 4736 6884 7058 7449 7476 8821 335
Size: 963 2148 174 391 27 1345 813
Fragments arranged by size:
2148 1380 1345 963 813 705 459 391
327 185 174 171 157 54 27
EagI C'GGCC_G
Cuts at: 2393 6884 2393
Size: 4491 4808
EarI CTCTTCn'nnn_
Cuts at: 1148 2419 2596 2771 3598 3748 4787 4970 7322
Size: 1271 177 175 827 150 1039 183 2352
Cuts at: 7322 7532 1148
Size: 210 2915
Fragments arranged by size:
2915 2352 1271 1039 827 210 183 177
175 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 183 2302 3294 3478 3958 4035 7507 7817 7963
Size: 2119 992 184 480 77 3472 310 146
Cuts at: 7963 9109 183
Size: 1146 373
Fragments arranged by size:
3472 2119 1146 992 480 373 310 184
146 77
EcoICRI GAG'CTC
Cuts at: 1062 1071 1236 1962 2412 4524 4692 6311 1062
Size: 9 165 726 450 2112 168 1619 4050
Fragments arranged by size:
4050 2112 1619 726 450 168 165 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 414 808 830 1366 1885 2192 2263 2392
Size: 161 394 22 536 519 307 71 129
Cuts at: 2392 2839 3118 3604 3619 3685 4006 4153 4264
Size: 447 279 486 15 66 321 147 111
Cuts at: 4264 4276 4336 4483 4513 4639 4678 4786 4870
Size: 12 60 147 30 126 39 108 84
Cuts at: 4870 5041 5119 5170 5221 5320 5335 5389 5509
Size: 171 78 51 51 99 15 54 120
Cuts at: 5509 5566 5626 5638 5668 5857 5878 6073 6201
Size: 57 60 12 30 189 21 195 128
Cuts at: 6201 7124 7556 8303 8571 253
Size: 923 432 747 268 981
Fragments arranged by size:
981 923 747 536 519 486 447 432
394 321 307 279 268 195 189 171
161 147 147 129 128 126 120 111
108 99 84 78 71 66 60 60
57 54 51 51 39 30 30 22
21 15 15 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 668 683 905 4603 668
Size: 15 222 3698 5364
Fragments arranged by size:
5364 3698 222 15
EcoO109I rG'GnC_Cy
Cuts at: 855 856 918 1102 1472 1473 2319 2320 2757
Size: 1 62 184 370 1 846 1 437
Cuts at: 2757 3768 3857 4362 4761 5094 5112 5439 5916
Size: 1011 89 505 399 333 18 327 477
Cuts at: 5916 5964 6100 6438 855
Size: 48 136 338 3716
Fragments arranged by size:
3716 1011 846 505 477 437 399 370
338 333 327 184 136 89 62 48
18 1 1 1
EcoRI G'AATT_C
Cuts at: 6303 6303
Size: 9299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7318 7318
Size: 9299
FseI GG_CCGG'CC
Cuts at: 2399 2399
Size: 9299
FspI TGC'GCA
Cuts at: 3442 7080 3442
Size: 3638 5661
FspAI rTGC'GCAy
Cuts at: 3442 3442
Size: 9299
HaeII r_GCGC'y
Cuts at: 540 575 1410 1418 1890 2301 2826 5314 6733
Size: 35 835 8 472 411 525 2488 1419
Cuts at: 6733 6981 8003 8982 540
Size: 248 1022 979 857
Fragments arranged by size:
2488 1419 1022 979 857 835 525 472
411 248 35 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 355 387 970 1002 1189 1221 1342
Size: 32 130 32 583 32 187 32 121
Cuts at: 1342 1374 1575 1607 2236 2268 2692 2724 3334
Size: 32 201 32 629 32 424 32 610
Cuts at: 3334 3366 3994 4026 4884 4916 5056 5088 5442
Size: 32 628 32 858 32 140 32 354
Cuts at: 5442 5474 5985 6017 6028 6060 6199 6231 7141
Size: 32 511 32 11 32 139 32 910
Cuts at: 7141 7171 7173 7203 193
Size: 30 2 30 2289
Fragments arranged by size:
2289 910 858 629 628 610 583 511
424 354 201 187 140 139 130 121
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
30 30 11 2
HincII GTy'rAC
Cuts at: 735 1219 2490 6066 6338 735
Size: 484 1271 3576 272 3696
Fragments arranged by size:
3696 3576 1271 484 272
HindIII A'AGCT_T
Cuts at: 3838 3838
Size: 9299
KasI G'GCGC_C
Cuts at: 1886 6729 6977 1886
Size: 4843 248 4208
Fragments arranged by size:
4843 4208 248
KpnI G_GTAC'C
Cuts at: 6319 6319
Size: 9299
MluI A'CGCG_T
Cuts at: 8732 8732
Size: 9299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 338 2600 7970 8154 338
Size: 2262 5370 184 1483
Fragments arranged by size:
5370 2262 1483 184
MscI TGG'CCA
Cuts at: 337 508 1213 1672 1829 2014 3775 4738 7060
Size: 171 705 459 157 185 1761 963 2322
Cuts at: 7060 337
Size: 2576
Fragments arranged by size:
2576 2322 1761 963 705 459 185 171
157
MslI CAynn'nnrTG
Cuts at: 331 841 1433 1547 2074 2282 2373 2608 2921
Size: 510 592 114 527 208 91 235 313
Cuts at: 2921 3136 3160 3196 3442 3550 4822 7288 7415
Size: 215 24 36 246 108 1272 2466 127
Cuts at: 7415 331
Size: 2215
Fragments arranged by size:
2466 2215 1272 592 527 510 313 246
235 215 208 127 114 108 91 36
24
MspA1I CmG'CkG
Cuts at: 214 341 483 651 1646 1691 2534 3114 3416
Size: 127 142 168 995 45 843 580 302
Cuts at: 3416 3611 3675 3876 3921 4056 4192 4344 4392
Size: 195 64 201 45 135 136 152 48
Cuts at: 4392 4551 4566 4581 5199 5325 6410 6724 7084
Size: 159 15 15 618 126 1085 314 360
Cuts at: 7084 8097 8342 214
Size: 1013 245 1171
Fragments arranged by size:
1171 1085 1013 995 843 618 580 360
314 302 245 201 195 168 159 152
142 136 135 127 126 64 48 45
45 15 15
NaeI GCC'GGC
Cuts at: 2397 7480 2397
Size: 5083 4216
NarI GG'CG_CC
Cuts at: 1887 6730 6978 1887
Size: 4843 248 4208
Fragments arranged by size:
4843 4208 248
NcoI C'CATG_G
Cuts at: 2922 5555 2922
Size: 2633 6666
NgoMIV G'CCGG_C
Cuts at: 2395 7478 2395
Size: 5083 4216
NheI G'CTAG_C
Cuts at: 1417 5659 1417
Size: 4242 5057
NspI r_CATG'y
Cuts at: 1509 3141 3447 4596 6352 6852 7759 1509
Size: 1632 306 1149 1756 500 907 3049
Fragments arranged by size:
3049 1756 1632 1149 907 500 306
PasI CC'CwG_GG
Cuts at: 927 927
Size: 9299
PciI A'CATG_T
Cuts at: 1505 7755 1505
Size: 6250 3049
PflMI CCAn_nnn'nTGG
Cuts at: 1656 3771 4059 4491 4827 6158 1656
Size: 2115 288 432 336 1331 4797
Fragments arranged by size:
4797 2115 1331 432 336 288
PfoI T'CCnGG_A
Cuts at: 398 779 1289 1739 2816 3764 4694 4757 5543
Size: 381 510 450 1077 948 930 63 786
Cuts at: 5543 5765 398
Size: 222 3932
Fragments arranged by size:
3932 1077 948 930 786 510 450 381
222 63
PmeI GTTT'AAAC
Cuts at: 6298 6298
Size: 9299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 262 294 4340 4372 5197 5229 8438 8470 262
Size: 32 4046 32 825 32 3209 32 1091
Fragments arranged by size:
4046 3209 1091 825 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 918 1102 3768 4362 4761 5094 5439 5916 5964
Size: 184 2666 594 399 333 345 477 48
Cuts at: 5964 6100 918
Size: 136 4117
Fragments arranged by size:
4117 2666 594 477 399 345 333 184
136 48
PspOMI G'GGCC_C
Cuts at: 855 1472 2207 2319 855
Size: 617 735 112 7835
Fragments arranged by size:
7835 735 617 112
PstI C_TGCA'G
Cuts at: 482 2063 2239 2297 2585 3014 3383 3724 4115
Size: 1581 176 58 288 429 369 341 391
Cuts at: 4115 4370 4403 4565 4580 5282 5507 5882 5924
Size: 255 33 162 15 702 225 375 42
Cuts at: 5924 6346 7031 482
Size: 422 685 2750
Fragments arranged by size:
2750 1581 702 685 429 422 391 375
369 341 288 255 225 176 162 58
42 33 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9299
PvuII CAG'CTG
Cuts at: 651 1646 3114 3611 3876 3921 4056 4344 5199
Size: 995 1468 497 265 45 135 288 855
Cuts at: 5199 5325 6724 7084 651
Size: 126 1399 360 2866
Fragments arranged by size:
2866 1468 1399 995 855 497 360 288
265 135 126 45
RsrII CG'GwC_CG
Cuts at: 7494 7494
Size: 9299
SacI G_AGCT'C
Cuts at: 1064 1073 1238 1964 2414 4526 4694 6313 1064
Size: 9 165 726 450 2112 168 1619 4050
Fragments arranged by size:
4050 2112 1619 726 450 168 165 9
SacII CC_GC'GG
Cuts at: 2535 3417 2535
Size: 882 8417
SalI G'TCGA_C
Cuts at: 733 2488 6336 733
Size: 1755 3848 3696
Fragments arranged by size:
3848 3696 1755
SapI GCTCTTCn'nnn_
Cuts at: 2419 2596 3598 4787 7322 7532 2419
Size: 177 1002 1189 2535 210 4186
Fragments arranged by size:
4186 2535 1189 1002 210 177
SbfI CC_TGCA'GG
Cuts at: 3014 3724 4370 5882 5924 6346 3014
Size: 710 646 1512 42 422 5967
Fragments arranged by size:
5967 1512 710 646 422 42
ScaI AGT'ACT
Cuts at: 8816 8816
Size: 9299
SexAI A'CCwGG_T
Cuts at: 5048 5048
Size: 9299
SfcI C'TryA_G
Cuts at: 33 478 2059 2235 2293 2403 2581 3010 3205
Size: 445 1581 176 58 110 178 429 195
Cuts at: 3205 3272 3379 3720 4111 4366 4399 4561 4576
Size: 67 107 341 391 255 33 162 15
Cuts at: 4576 5278 5503 5752 5878 5920 6342 7027 8020
Size: 702 225 249 126 42 422 685 993
Cuts at: 8020 8211 33
Size: 191 1121
Fragments arranged by size:
1581 1121 993 702 685 445 429 422
391 341 255 249 225 195 191 178
176 162 126 110 107 67 58 42
33 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 863 863
Size: 9299
SfoI GGC'GCC
Cuts at: 1888 6731 6979 1888
Size: 4843 248 4208
Fragments arranged by size:
4843 4208 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9299
SmaI CCC'GGG
Cuts at: 2498 5437 6321 2498
Size: 2939 884 5476
Fragments arranged by size:
5476 2939 884
SmlI C'TyrA_G
Cuts at: 442 1039 2380 2449 3934 4408 5188 5668 6022
Size: 597 1341 69 1485 474 780 480 354
Cuts at: 6022 6454 7369 7861 8123 442
Size: 432 915 492 262 1618
Fragments arranged by size:
1618 1485 1341 915 780 597 492 480
474 432 354 262 69
SnaBI TAC'GTA
Cuts at: 6291 6291
Size: 9299
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9299
SphI G_CATG'C
Cuts at: 3141 6352 6852 3141
Size: 3211 500 5588
Fragments arranged by size:
5588 3211 500
StuI AGG'CCT
Cuts at: 871 1159 2107 2707 871
Size: 288 948 600 7463
Fragments arranged by size:
7463 948 600 288
StyI C'CwwG_G
Cuts at: 914 2922 3453 3653 4718 4739 5108 5555 5774
Size: 2008 531 200 1065 21 369 447 219
Cuts at: 5774 6107 6433 6787 914
Size: 333 326 354 3426
Fragments arranged by size:
3426 2008 1065 531 447 369 354 333
326 219 200 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2584 3004 3421 4678 6170 6882 7015 7474 7683
Size: 420 417 1257 1492 712 133 459 209
Cuts at: 7683 2584
Size: 4200
Fragments arranged by size:
4200 1492 1257 712 459 420 417 209
133
TatI w'GTAC_w
Cuts at: 418 1484 1730 2942 3786 3870 8814 418
Size: 1066 246 1212 844 84 4944 903
Fragments arranged by size:
4944 1212 1066 903 844 246 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 638 670 761 793 1165 1197 1245 1277 3176
Size: 32 91 32 372 32 48 32 1899
Cuts at: 3176 3208 8816 8848 638
Size: 32 5608 32 1089
Fragments arranged by size:
5608 1899 1089 372 91 48 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6070 7096 6070
Size: 1026 8273
XbaI T'CTAG_A
Cuts at: 639 4680 5847 6330 639
Size: 4041 1167 483 3608
Fragments arranged by size:
4041 3608 1167 483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 300 1293 2076 2136 2281 3093 3367 4458 4824
Size: 993 783 60 145 812 274 1091 366
Cuts at: 4824 4845 5115 5310 5781 6794 300
Size: 21 270 195 471 1013 2805
Fragments arranged by size:
2805 1091 1013 993 812 783 471 366
274 270 195 145 60 21
XhoI C'TCGA_G
Cuts at: 3934 3934
Size: 9299
XmaI C'CCGG_G
Cuts at: 2496 5435 6319 2496
Size: 2939 884 5476
Fragments arranged by size:
5476 2939 884
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AscI AvrII BfrBI BsiWI BssHII BstZ17I
ClaI EcoRV HpaI MfeI NdeI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SanDI SgrAI
SrfI SspI SwaI XmnI ZraI