Restriction map for
pF1KSDA0115
(Circular) (Six-base) MAPSORT of: pF1KSDA0115.seq Check: 5147 from: 1 to: 4507
pF1KSDA0115 4507 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 136 136
Size: 4507
AccI GT'mk_AC
Cuts at: 1543 1543
Size: 4507
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4507
AclI AA'CG_TT
Cuts at: 4347 4347
Size: 4507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 688 879 900 1249 1434 2330 2762 3511
Size: 342 191 21 349 185 896 432 749
Cuts at: 3511 3779 346
Size: 268 1074
Fragments arranged by size:
1074 896 749 432 349 342 268 191
185 21
AfeI AGC'GCT
Cuts at: 1384 1384
Size: 4507
AflIII A'CryG_T
Cuts at: 1274 1857 2963 3940 1274
Size: 583 1106 977 1841
Fragments arranged by size:
1841 1106 977 583
AgeI A'CCGG_T
Cuts at: 2895 2895
Size: 4507
AleI CACnn'nnGTG
Cuts at: 268 268
Size: 4507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 286 286
Size: 4507
AlwNI CAG_nnn'CTG
Cuts at: 151 1810 3379 151
Size: 1659 1569 1279
Fragments arranged by size:
1659 1569 1279
ApaI G_GGCC'C
Cuts at: 210 210
Size: 4507
ApaLI G'TGCA_C
Cuts at: 3277 3277
Size: 4507
ApoI r'AATT_y
Cuts at: 397 1509 3860 4116 397
Size: 1112 2351 256 788
Fragments arranged by size:
2351 1112 788 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4507
AvaI C'yCGr_G
Cuts at: 197 1525 197
Size: 1328 3179
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1331 1364 1331
Size: 33 4474
BamHI G'GATC_C
Cuts at: 1530 1530
Size: 4507
BanI G'GyrC_C
Cuts at: 954 1521 1935 2183 2218 954
Size: 567 414 248 35 3243
Fragments arranged by size:
3243 567 414 248 35
BanII G_rGCy'C
Cuts at: 185 210 347 778 866 1442 1519 2549 185
Size: 25 137 431 88 576 77 1030 2143
Fragments arranged by size:
2143 1030 576 431 137 88 77 25
BbeI G_GCGC'C
Cuts at: 958 1939 2187 958
Size: 981 248 3278
Fragments arranged by size:
3278 981 248
BbsI GAAGACnn'nnnn_
Cuts at: 1452 1452
Size: 4507
BciVI GTATCCnnnnn_n'
Cuts at: 833 1359 2403 3166 4507 833
Size: 526 1044 763 1341 833
Fragments arranged by size:
1341 1044 833 763 526
BclI T'GATC_A
Cuts at: 2025 2025
Size: 4507
BglII A'GATC_T
Cuts at: 2020 4059 2020
Size: 2039 2468
BlpI GC'TnA_GC
Cuts at: 1617 1617
Size: 4507
Bme1580I G_kGCm'C
Cuts at: 210 2130 2223 3281 210
Size: 1920 93 1058 1436
Fragments arranged by size:
1920 1436 1058 93
BmgBI CAC'GTC
Cuts at: 1275 1275
Size: 4507
BmrI ACTGGGnnnn_n'
Cuts at: 813 1717 1798 2133 4279 813
Size: 904 81 335 2146 1041
Fragments arranged by size:
2146 1041 904 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1210 1348 850
Size: 360 138 4009
Fragments arranged by size:
4009 360 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 2560 3054 3352 1681
Size: 879 494 298 2836
Fragments arranged by size:
2836 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 4211 460
Size: 3751 756
BsaAI yAC'GTr
Cuts at: 1497 1858 1497
Size: 361 4146
BsaBI GATnn'nnATC
Cuts at: 6 2019 4058 6
Size: 2013 2039 455
Fragments arranged by size:
2039 2013 455
BsaHI Gr'CG_yC
Cuts at: 955 1936 2184 2888 4384 955
Size: 981 248 704 1496 1078
Fragments arranged by size:
1496 1078 981 704 248
BsaWI w'CCGG_w
Cuts at: 137 317 1694 2215 2895 3169 3316 3727 137
Size: 180 1377 521 680 274 147 411 917
Fragments arranged by size:
1377 917 680 521 411 274 180 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 485 515 485
Size: 30 4477
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 592 841 1045 183
Size: 409 249 204 3645
Fragments arranged by size:
3645 409 249 204
BseYI C'CCAG_C
Cuts at: 151 184 370 546 859 1016 1434 1930 2688
Size: 33 186 176 313 157 418 496 758
Cuts at: 2688 3267 3763 151
Size: 579 496 895
Fragments arranged by size:
895 758 579 496 496 418 313 186
176 157 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 964 164
Size: 800 3707
BsiEI CG_ry'CG
Cuts at: 104 2047 2093 3303 104
Size: 1943 46 1210 1308
Fragments arranged by size:
1943 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 347 1519 2297 2487 3281 347
Size: 1172 778 190 794 1573
Fragments arranged by size:
1573 1172 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 185 210 347 778 866 1442 1519 2130 2223
Size: 25 137 431 88 576 77 611 93
Cuts at: 2223 2297 2487 2549 3281 185
Size: 74 190 62 732 1411
Fragments arranged by size:
1411 732 611 576 431 190 137 93
88 77 74 62 25
BspEI T'CCGG_A
Cuts at: 317 3727 317
Size: 3410 1097
BspHI T'CATG_A
Cuts at: 3683 3683
Size: 4507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 136 671 1555 1769 2071 2452 136
Size: 535 884 214 302 381 2191
Fragments arranged by size:
2191 884 535 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2797 2851 4337 4502 50
Size: 2747 54 1486 165 55
Fragments arranged by size:
2747 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 854 2417 854
Size: 1563 2944
BsrFI r'CCGG_y
Cuts at: 1310 2503 2684 2895 1310
Size: 1193 181 211 2922
Fragments arranged by size:
2922 1193 211 181
BssSI C'ACGA_G
Cuts at: 2776 3136 2776
Size: 360 4147
BstAPI GCAn_nnn'nTGC
Cuts at: 557 1882 557
Size: 1325 3182
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4507
BstEII G'GTnAC_C
Cuts at: 529 529
Size: 4507
BstXI CCAn_nnnn'nTGG
Cuts at: 1434 1434
Size: 4507
BstYI r'GATC_y
Cuts at: 1 1530 1997 2020 2355 2601 3615 3701 3713
Size: 1529 467 23 335 246 1014 86 12
Cuts at: 3713 4059 1
Size: 346 449
Fragments arranged by size:
1529 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 191 125
Size: 66 4441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1190 2455 2636 2799 1190
Size: 1265 181 163 2898
Fragments arranged by size:
2898 1265 181 163
BtsI GCAGTG_nn'
Cuts at: 504 558 947 1748 504
Size: 54 389 801 3263
Fragments arranged by size:
3263 801 389 54
DraI TTT'AAA
Cuts at: 1296 1504 1296
Size: 208 4299
DrdI GACnn_nn'nnGTC
Cuts at: 2211 3071 2211
Size: 860 3647
EaeI y'GGCC_r
Cuts at: 627 734 1357 2090 2264 2655 2682 4029 627
Size: 107 623 733 174 391 27 1347 1105
Fragments arranged by size:
1347 1105 733 623 391 174 107 27
EagI C'GGCC_G
Cuts at: 2090 2090
Size: 4507
EarI CTCTTCn'nnn_
Cuts at: 542 783 1341 1447 2528 2738 542
Size: 241 558 106 1081 210 2311
Fragments arranged by size:
2311 1081 558 241 210 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 506 2713 3025 3171 4317 506
Size: 2207 312 146 1146 696
Fragments arranged by size:
2207 1146 696 312 146
EcoICRI GAG'CTC
Cuts at: 345 1517 345
Size: 1172 3335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 688 850 879 900 1210 1249 1348 1434
Size: 342 162 29 21 310 39 99 86
Cuts at: 1434 2330 2762 3511 3779 346
Size: 896 432 749 268 1074
Fragments arranged by size:
1074 896 749 432 342 310 268 162
99 86 39 29 21
EcoNI CCTnn'n_nnAGG
Cuts at: 162 667 162
Size: 505 4002
EcoO109I rG'GnC_Cy
Cuts at: 155 207 672 1051 1161 1220 1644 155
Size: 52 465 379 110 59 424 3018
Fragments arranged by size:
3018 465 424 379 110 59 52
EcoRI G'AATT_C
Cuts at: 397 1509 397
Size: 1112 3395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4507
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4507
HaeII r_GCGC'y
Cuts at: 958 1386 1421 1939 2187 3211 4190 958
Size: 428 35 518 248 1024 979 1275
Fragments arranged by size:
1275 1024 979 518 428 248 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 440 472 2347 2377 2379 2409 440
Size: 32 1875 30 2 30 2538
Fragments arranged by size:
2538 1875 32 30 30 2
HincII GTy'rAC
Cuts at: 1544 1544
Size: 4507
KasI G'GCGC_C
Cuts at: 954 1935 2183 954
Size: 981 248 3278
Fragments arranged by size:
3278 981 248
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4507
MluI A'CGCG_T
Cuts at: 3940 3940
Size: 4507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 545 1514 3178 3362 545
Size: 969 1664 184 1690
Fragments arranged by size:
1690 1664 969 184
MscI TGG'CCA
Cuts at: 629 2266 629
Size: 1637 2870
MslI CAynn'nnrTG
Cuts at: 268 460 608 2494 2621 268
Size: 192 148 1886 127 2154
Fragments arranged by size:
2154 1886 192 148 127
MspA1I CmG'CkG
Cuts at: 193 254 361 899 1016 1188 1616 1930 2290
Size: 61 107 538 117 172 428 314 360
Cuts at: 2290 3305 3550 193
Size: 1015 245 1150
Fragments arranged by size:
1150 1015 538 428 360 314 245 172
117 107 61
NaeI GCC'GGC
Cuts at: 2686 2686
Size: 4507
NarI GG'CG_CC
Cuts at: 955 1936 2184 955
Size: 981 248 3278
Fragments arranged by size:
3278 981 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4507
NgoMIV G'CCGG_C
Cuts at: 2684 2684
Size: 4507
NruI TCG'CGA
Cuts at: 2868 2868
Size: 4507
NspI r_CATG'y
Cuts at: 832 1558 2058 2967 832
Size: 726 500 909 2372
Fragments arranged by size:
2372 909 726 500
PciI A'CATG_T
Cuts at: 2963 2963
Size: 4507
PfoI T'CCnGG_A
Cuts at: 800 800
Size: 4507
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3646 3678 3646
Size: 32 4475
PpuMI rG'GwC_Cy
Cuts at: 155 1161 1220 155
Size: 1006 59 3442
Fragments arranged by size:
3442 1006 59
PspOMI G'GGCC_C
Cuts at: 206 206
Size: 4507
PstI C_TGCA'G
Cuts at: 1552 2237 1552
Size: 685 3822
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4507
PvuII CAG'CTG
Cuts at: 1188 1930 2290 1188
Size: 742 360 3405
Fragments arranged by size:
3405 742 360
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4507
SacI G_AGCT'C
Cuts at: 347 1519 347
Size: 1172 3335
SacII CC_GC'GG
Cuts at: 194 194
Size: 4507
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4507
SanDI GG'GwC_CC
Cuts at: 1161 1161
Size: 4507
SapI GCTCTTCn'nnn_
Cuts at: 783 1447 2528 2738 783
Size: 664 1081 210 2552
Fragments arranged by size:
2552 1081 664 210
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4507
ScaI AGT'ACT
Cuts at: 4024 4024
Size: 4507
SfcI C'TryA_G
Cuts at: 33 1548 2233 3228 3419 33
Size: 1515 685 995 191 1121
Fragments arranged by size:
1515 1121 995 685 191
SfoI GGC'GCC
Cuts at: 956 1937 2185 956
Size: 981 248 3278
Fragments arranged by size:
3278 981 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4507
SmaI CCC'GGG
Cuts at: 199 1527 199
Size: 1328 3179
SmlI C'TyrA_G
Cuts at: 1660 2575 3069 3331 1660
Size: 915 494 262 2836
Fragments arranged by size:
2836 915 494 262
SnaBI TAC'GTA
Cuts at: 1497 1497
Size: 4507
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4507
SphI G_CATG'C
Cuts at: 1558 2058 1558
Size: 500 4007
SrfI GCCC'GGGC
Cuts at: 199 199
Size: 4507
StyI C'CwwG_G
Cuts at: 125 624 1639 1993 125
Size: 499 1015 354 2639
Fragments arranged by size:
2639 1015 499 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 832 1008 1104 2088 2221 2680 2891 832
Size: 176 96 984 133 459 211 2448
Fragments arranged by size:
2448 984 459 211 176 133 96
TatI w'GTAC_w
Cuts at: 1331 4022 1331
Size: 2691 1816
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 485 517 807 839 4024 4056 485
Size: 32 290 32 3185 32 936
Fragments arranged by size:
3185 936 290 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2302 2302
Size: 4507
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2000 2000
Size: 4507
XmaI C'CCGG_G
Cuts at: 197 1525 197
Size: 1328 3179
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BbvCI BcgI
BfrBI BglI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SexAI SfiI SgrAI SspI StuI SwaI XhoI XmnI
ZraI