Restriction map for
pF1KSDA0083
(Circular) (Six-base) MAPSORT of: pF1KSDA0083.seq Check: 4119 from: 1 to: 6317
pF1KSDA0083 6317 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1657 1657
Size: 6317
AatII G_ACGT'C
Cuts at: 3123 3123
Size: 6317
AccI GT'mk_AC
Cuts at: 3023 3355 3023
Size: 332 5985
Acc65I G'GTAC_C
Cuts at: 263 3333 263
Size: 3070 3247
AclI AA'CG_TT
Cuts at: 6157 6157
Size: 6317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 4142 4574 5321 5589 1444
Size: 2698 432 747 268 2172
Fragments arranged by size:
2698 2172 747 432 268
AflII C'TTAA_G
Cuts at: 2631 2631
Size: 6317
AflIII A'CryG_T
Cuts at: 874 1584 1877 2350 3669 4773 5750 874
Size: 710 293 473 1319 1104 977 1441
Fragments arranged by size:
1441 1319 1104 977 710 473 293
AgeI A'CCGG_T
Cuts at: 260 4705 260
Size: 4445 1872
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 350 382 350
Size: 32 6285
AlwNI CAG_nnn'CTG
Cuts at: 1114 1422 3622 5189 1114
Size: 308 2200 1567 2242
Fragments arranged by size:
2242 2200 1567 308
ApaI G_GGCC'C
Cuts at: 2449 2449
Size: 6317
ApaLI G'TGCA_C
Cuts at: 5087 5087
Size: 6317
ApoI r'AATT_y
Cuts at: 1041 2114 2273 2387 2737 3321 5670 5926 1041
Size: 1073 159 114 350 584 2349 256 1432
Fragments arranged by size:
2349 1432 1073 584 350 256 159 114
AseI AT'TA_AT
Cuts at: 20 3238 20
Size: 3218 3099
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
AvaI C'yCGr_G
Cuts at: 3337 3337
Size: 6317
AvrII C'CTAG_G
Cuts at: 928 928
Size: 6317
BamHI G'GATC_C
Cuts at: 3342 3342
Size: 6317
BanI G'GyrC_C
Cuts at: 263 505 1639 3333 3747 3995 4030 263
Size: 242 1134 1694 414 248 35 2550
Fragments arranged by size:
2550 1694 1134 414 248 242 35
BanII G_rGCy'C
Cuts at: 571 2449 2532 3331 4361 571
Size: 1878 83 799 1030 2527
Fragments arranged by size:
2527 1878 1030 799 83
BbeI G_GCGC'C
Cuts at: 3751 3999 3751
Size: 248 6069
BbsI GAAGACnn'nnnn_
Cuts at: 1261 1261
Size: 6317
BciVI GTATCCnnnnn_n'
Cuts at: 494 1968 4215 4976 6317 494
Size: 1474 2247 761 1341 494
Fragments arranged by size:
2247 1474 1341 761 494
BclI T'GATC_A
Cuts at: 3837 3837
Size: 6317
BfrBI ATG'CAT
Cuts at: 365 801 365
Size: 436 5881
BglI GCCn_nnn'nGGC
Cuts at: 2016 2016
Size: 6317
BglII A'GATC_T
Cuts at: 3832 5869 3832
Size: 2037 4280
BlpI GC'TnA_GC
Cuts at: 3429 3429
Size: 6317
Bme1580I G_kGCm'C
Cuts at: 2449 3179 3942 4035 5091 2449
Size: 730 763 93 1056 3675
Fragments arranged by size:
3675 1056 763 730 93
BmrI ACTGGGnnnn_n'
Cuts at: 3295 3529 3610 3945 6089 3295
Size: 234 81 335 2144 3523
Fragments arranged by size:
3523 2144 335 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4684 4684
Size: 6317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1495 2584 2683 166
Size: 1329 1089 99 3800
Fragments arranged by size:
3800 1329 1089 99
Bpu10I CC'TnA_GC
Cuts at: 2301 2301
Size: 6317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 3493 4372 4864 5162 1117
Size: 2376 879 492 298 2272
Fragments arranged by size:
2376 2272 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6021 6021
Size: 6317
BsaAI yAC'GTr
Cuts at: 3309 3670 3309
Size: 361 5956
BsaBI GATnn'nnATC
Cuts at: 6 3831 5868 6
Size: 3825 2037 455
Fragments arranged by size:
3825 2037 455
BsaHI Gr'CG_yC
Cuts at: 3120 3748 3996 4698 6194 3120
Size: 628 248 702 1496 3243
Fragments arranged by size:
3243 1496 702 628 248
BsaWI w'CCGG_w
Cuts at: 260 3506 4027 4705 4979 5126 5537 260
Size: 3246 521 678 274 147 411 1040
Fragments arranged by size:
3246 1040 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 190
Size: 6317
BseYI C'CCAG_C
Cuts at: 1415 3742 4500 5077 5573 1415
Size: 2327 758 577 496 2159
Fragments arranged by size:
2327 2159 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 2620 417
Size: 2203 4114
BsiEI CG_ry'CG
Cuts at: 104 3859 3905 5113 104
Size: 3755 46 1208 1308
Fragments arranged by size:
3755 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 247 571 1335 2532 3331 4109 4299 5091 247
Size: 324 764 1197 799 778 190 792 1473
Fragments arranged by size:
1473 1197 799 792 778 764 324 190
BsmI GAATG_Cn'
Cuts at: 1210 1988 1210
Size: 778 5539
Bsp1286I G_dGCh'C
Cuts at: 247 571 1335 2449 2532 3179 3331 3942 4035
Size: 324 764 1114 83 647 152 611 93
Cuts at: 4035 4109 4299 4361 5091 247
Size: 74 190 62 730 1473
Fragments arranged by size:
1473 1114 764 730 647 611 324 190
152 93 83 74 62
BspEI T'CCGG_A
Cuts at: 5537 5537
Size: 6317
BspHI T'CATG_A
Cuts at: 5493 5493
Size: 6317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1657 3367 3581 3883 4264 1657
Size: 1710 214 302 381 3710
Fragments arranged by size:
3710 1710 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4609 4663 6147 6312 50
Size: 4559 54 1484 165 55
Fragments arranged by size:
4559 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2868 4229 2868
Size: 1361 4956
BsrFI r'CCGG_y
Cuts at: 189 260 4315 4496 4705 189
Size: 71 4055 181 209 1801
Fragments arranged by size:
4055 1801 209 181 71
BsrGI T'GTAC_A
Cuts at: 288 1073 2586 288
Size: 785 1513 4019
Fragments arranged by size:
4019 1513 785
BssSI C'ACGA_G
Cuts at: 4588 4946 4588
Size: 358 5959
BstAPI GCAn_nnn'nTGC
Cuts at: 3694 3694
Size: 6317
BstBI TT'CG_AA
Cuts at: 110 4678 110
Size: 4568 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1869 1869
Size: 6317
BstYI r'GATC_y
Cuts at: 1 3342 3809 3832 4167 4413 5425 5511 5523
Size: 3341 467 23 335 246 1012 86 12
Cuts at: 5523 5869 1
Size: 346 449
Fragments arranged by size:
3341 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 370 370
Size: 6317
BtgI C'CryG_G
Cuts at: 125 125
Size: 6317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2038 4267 4448 4611 2038
Size: 2229 181 163 3744
Fragments arranged by size:
3744 2229 181 163
BtsI GCAGTG_nn'
Cuts at: 329 2699 3560 329
Size: 2370 861 3086
Fragments arranged by size:
3086 2370 861
DraI TTT'AAA
Cuts at: 2202 2715 3224 3316 2202
Size: 513 509 92 5203
Fragments arranged by size:
5203 513 509 92
DrdI GACnn_nn'nnGTC
Cuts at: 4023 4881 4023
Size: 858 5459
EaeI y'GGCC_r
Cuts at: 2684 3902 4076 4467 4494 5839 2684
Size: 1218 174 391 27 1345 3162
Fragments arranged by size:
3162 1345 1218 391 174 27
EagI C'GGCC_G
Cuts at: 3902 3902
Size: 6317
EarI CTCTTCn'nnn_
Cuts at: 157 534 1092 1286 1540 2312 4340 4550 157
Size: 377 558 194 254 772 2028 210 1924
Fragments arranged by size:
2028 1924 772 558 377 254 210 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 208 4525 4835 4981 6127 196
Size: 12 4317 310 146 1146 386
Fragments arranged by size:
4317 1146 386 310 146 12
EcoICRI GAG'CTC
Cuts at: 569 2530 3329 569
Size: 1961 799 3557
Fragments arranged by size:
3557 1961 799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1444 1495 2584 2683 4142 4574 5321 5589
Size: 1278 51 1089 99 1459 432 747 268
Cuts at: 5589 166
Size: 894
Fragments arranged by size:
1459 1278 1089 894 747 432 268 99
51
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 2078
Size: 6317
EcoO109I rG'GnC_Cy
Cuts at: 2446 3456 2446
Size: 1010 5307
EcoRI G'AATT_C
Cuts at: 2114 3321 2114
Size: 1207 5110
EcoRV GAT'ATC
Cuts at: 411 411
Size: 6317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4336 4336
Size: 6317
FspI TGC'GCA
Cuts at: 4098 4098
Size: 6317
HaeII r_GCGC'y
Cuts at: 321 2833 3751 3999 5021 6000 321
Size: 2512 918 248 1022 979 638
Fragments arranged by size:
2512 1022 979 918 638 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 453 468 500 558 590 1468 1500 2088
Size: 32 15 32 58 32 878 32 588
Cuts at: 2088 2120 2401 2433 2656 2688 3238 3270 4159
Size: 32 281 32 223 32 550 32 889
Cuts at: 4159 4189 4191 4221 421
Size: 30 2 30 2517
Fragments arranged by size:
2517 889 878 588 550 281 223 58
32 32 32 32 32 32 32 32
30 30 15 2
HincII GTy'rAC
Cuts at: 1469 2147 2172 2332 2594 3356 1469
Size: 678 25 160 262 762 4430
Fragments arranged by size:
4430 762 678 262 160 25
HindIII A'AGCT_T
Cuts at: 2547 2547
Size: 6317
HpaI GTT'AAC
Cuts at: 1469 2594 1469
Size: 1125 5192
KasI G'GCGC_C
Cuts at: 3747 3995 3747
Size: 248 6069
KpnI G_GTAC'C
Cuts at: 267 3337 267
Size: 3070 3247
MfeI C'AATT_G
Cuts at: 1326 1382 1326
Size: 56 6261
MluI A'CGCG_T
Cuts at: 5750 5750
Size: 6317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4988 5172 4988
Size: 184 6133
MscI TGG'CCA
Cuts at: 2686 4078 2686
Size: 1392 4925
MslI CAynn'nnrTG
Cuts at: 250 4306 4433 250
Size: 4056 127 2134
Fragments arranged by size:
4056 2134 127
MspA1I CmG'CkG
Cuts at: 135 1014 3428 3742 4102 5115 5360 135
Size: 879 2414 314 360 1013 245 1092
Fragments arranged by size:
2414 1092 1013 879 360 314 245
NaeI GCC'GGC
Cuts at: 191 4498 191
Size: 4307 2010
NarI GG'CG_CC
Cuts at: 3748 3996 3748
Size: 248 6069
NcoI C'CATG_G
Cuts at: 125 125
Size: 6317
NgoMIV G'CCGG_C
Cuts at: 189 4496 189
Size: 4307 2010
NsiI A_TGCA'T
Cuts at: 367 803 367
Size: 436 5881
NspI r_CATG'y
Cuts at: 495 878 1588 2354 3370 3870 4777 495
Size: 383 710 766 1016 500 907 2035
Fragments arranged by size:
2035 1016 907 766 710 500 383
PciI A'CATG_T
Cuts at: 874 1584 2350 4773 874
Size: 710 766 2423 2418
Fragments arranged by size:
2423 2418 766 710
PflMI CCAn_nnn'nTGG
Cuts at: 1114 3285 1114
Size: 2171 4146
PmeI GTTT'AAAC
Cuts at: 3316 3316
Size: 6317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5456 5488 5456
Size: 32 6285
PspOMI G'GGCC_C
Cuts at: 2445 2445
Size: 6317
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1296 1328 1296
Size: 32 6285
PstI C_TGCA'G
Cuts at: 3364 4049 3364
Size: 685 5632
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6317
PvuII CAG'CTG
Cuts at: 135 3742 4102 135
Size: 3607 360 2350
Fragments arranged by size:
3607 2350 360
RsrII CG'GwC_CG
Cuts at: 4512 4512
Size: 6317
SacI G_AGCT'C
Cuts at: 571 2532 3331 571
Size: 1961 799 3557
Fragments arranged by size:
3557 1961 799
SalI G'TCGA_C
Cuts at: 3354 3354
Size: 6317
SapI GCTCTTCn'nnn_
Cuts at: 534 1092 2312 4340 4550 534
Size: 558 1220 2028 210 2301
Fragments arranged by size:
2301 2028 1220 558 210
SbfI CC_TGCA'GG
Cuts at: 3364 3364
Size: 6317
ScaI AGT'ACT
Cuts at: 5834 5834
Size: 6317
SexAI A'CCwGG_T
Cuts at: 266 266
Size: 6317
SfcI C'TryA_G
Cuts at: 33 3360 4045 5038 5229 33
Size: 3327 685 993 191 1121
Fragments arranged by size:
3327 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3749 3997 3749
Size: 248 6069
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
SmaI CCC'GGG
Cuts at: 3339 3339
Size: 6317
SmlI C'TyrA_G
Cuts at: 1132 2631 3472 4387 4879 5141 1132
Size: 1499 841 915 492 262 2308
Fragments arranged by size:
2308 1499 915 841 492 262
SnaBI TAC'GTA
Cuts at: 3309 3309
Size: 6317
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6317
SphI G_CATG'C
Cuts at: 3370 3870 3370
Size: 500 5817
SspI AAT'ATT
Cuts at: 1309 1444 1612 2178 2375 1309
Size: 135 168 566 197 5251
Fragments arranged by size:
5251 566 197 168 135
StyI C'CwwG_G
Cuts at: 125 928 2764 3033 3451 3805 125
Size: 803 1836 269 418 354 2637
Fragments arranged by size:
2637 1836 803 418 354 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1770 3277 3900 4033 4492 4701 1770
Size: 1507 623 133 459 209 3386
Fragments arranged by size:
3386 1507 623 459 209 133
TatI w'GTAC_w
Cuts at: 288 1073 2101 2586 2603 5832 288
Size: 785 1028 485 17 3229 773
Fragments arranged by size:
3229 1028 785 773 485 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5834 5866 5834
Size: 32 6285
Tth111I GACn'n_nGTC
Cuts at: 4114 4114
Size: 6317
XbaI T'CTAG_A
Cuts at: 1170 1717 2310 3348 1170
Size: 547 593 1038 4139
Fragments arranged by size:
4139 1038 593 547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 595 1633 3812 595
Size: 1038 2179 3100
Fragments arranged by size:
3100 2179 1038
XmaI C'CCGG_G
Cuts at: 3337 3337
Size: 6317
XmnI GAAnn'nnTTC
Cuts at: 1857 1857
Size: 6317
ZraI GAC'GTC
Cuts at: 3121 3121
Size: 6317
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AscI BaeI BbvCI BcgI
BmgBI BmtI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI CspCI DraIII FseI FspAI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspXI SacII SanDI SfiI SgrAI SrfI StuI SwaI
XhoI