Restriction map for
pF1KF0165
(Circular) (Six-base) MAPSORT of: pF1KF0165.seq Check: 6728 from: 1 to: 3923
pF1KF0165 3923 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 764 961 764
Size: 197 3726
Acc65I G'GTAC_C
Cuts at: 566 939 566
Size: 373 3550
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 737 1748 2180 2533 3195 279
Size: 458 1011 432 353 662 1007
Fragments arranged by size:
1011 1007 662 458 432 353
AflIII A'CryG_T
Cuts at: 1275 3075 3356 1275
Size: 1800 281 1842
Fragments arranged by size:
1842 1800 281
AleI CACnn'nnGTG
Cuts at: 460 460
Size: 3923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 760 1228 2666 760
Size: 468 1438 2017
Fragments arranged by size:
2017 1438 468
ApaI G_GGCC'C
Cuts at: 138 138
Size: 3923
ApaLI G'TGCA_C
Cuts at: 2761 2761
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 208 943 208
Size: 735 3188
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 487 538 566 939 1353 1601 1636 487
Size: 51 28 373 414 248 35 2774
Fragments arranged by size:
2774 414 373 248 51 35 28
BanII G_rGCy'C
Cuts at: 138 167 385 578 850 937 1967 138
Size: 29 218 193 272 87 1030 2094
Fragments arranged by size:
2094 1030 272 218 193 87 29
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 781 815 781
Size: 34 3889
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2877 3923 1821
Size: 1056 1046 1821
Fragments arranged by size:
1821 1056 1046
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglI GCCn_nnn'nGGC
Cuts at: 274 274
Size: 3923
BglII A'GATC_T
Cuts at: 363 724 1438 3475 363
Size: 361 714 2037 811
Fragments arranged by size:
2037 811 714 361
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 138 421 649 715 1548 1641 2765 138
Size: 283 228 66 833 93 1124 1296
Fragments arranged by size:
1296 1124 833 283 228 93 66
BmrI ACTGGGnnnn_n'
Cuts at: 424 1135 1216 1551 3695 424
Size: 711 81 335 2144 652
Fragments arranged by size:
2144 711 652 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 352
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1978 2692 2990 1099
Size: 879 714 298 2032
Fragments arranged by size:
2032 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 781 858 3627 781
Size: 77 2769 1077
Fragments arranged by size:
2769 1077 77
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 1354 1602 2304 3800 1354
Size: 248 702 1496 1477
Fragments arranged by size:
1496 1477 702 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2722 2869 3143 1112
Size: 521 678 411 147 274 1892
Fragments arranged by size:
1892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 581 611 581
Size: 30 3893
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 393 175
Size: 218 3705
BseYI C'CCAG_C
Cuts at: 1348 2106 2771 3179 1348
Size: 758 665 408 2092
Fragments arranged by size:
2092 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2741 104
Size: 1361 46 1230 1286
Fragments arranged by size:
1361 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 937 1715 1905 2765 937
Size: 778 190 860 2095
Fragments arranged by size:
2095 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 551 551
Size: 3923
Bsp1286I G_dGCh'C
Cuts at: 138 167 385 421 578 649 715 850 937
Size: 29 218 36 157 71 66 135 87
Cuts at: 937 1548 1641 1715 1905 1967 2765 138
Size: 611 93 74 190 62 798 1296
Fragments arranged by size:
1296 798 611 218 190 157 135 93
87 74 71 66 62 36 29
BspHI T'CATG_A
Cuts at: 357 2355 357
Size: 1998 1925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1187 1489 1870 973
Size: 214 302 381 3026
Fragments arranged by size:
3026 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 250 616 1921 2102 250
Size: 366 1305 181 2071
Fragments arranged by size:
2071 1305 366 181
BssSI C'ACGA_G
Cuts at: 2194 2902 2194
Size: 708 3215
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 878 2284 878
Size: 1406 2517
BstEII G'GTnAC_C
Cuts at: 453 453
Size: 3923
BstYI r'GATC_y
Cuts at: 1 363 717 724 948 1415 1438 1773 2019
Size: 362 354 7 224 467 23 335 246
Cuts at: 2019 2325 2337 2423 3475 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 362 354 335 306 246
224 86 23 12 7
BtgI C'CryG_G
Cuts at: 107 521 107
Size: 414 3509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 451 1629 2973 451
Size: 1178 1344 1401
Fragments arranged by size:
1401 1344 1178
EaeI y'GGCC_r
Cuts at: 275 479 511 1508 1682 2073 2100 3445 275
Size: 204 32 997 174 391 27 1345 753
Fragments arranged by size:
1345 997 753 391 204 174 32 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2131 2873 3019 3733 2131
Size: 742 146 714 2321
Fragments arranged by size:
2321 742 714 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 352 737 1748 2180 2533 3195 279
Size: 73 385 1011 432 353 662 1007
Fragments arranged by size:
1011 1007 662 432 385 353 73
EcoNI CCTnn'n_nnAGG
Cuts at: 856 856
Size: 3923
EcoO109I rG'GnC_Cy
Cuts at: 135 212 696 1062 135
Size: 77 484 366 2996
Fragments arranged by size:
2996 484 366 77
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 783 1357 1605 2835 3606 783
Size: 574 248 1230 771 1100
Fragments arranged by size:
1230 1100 771 574 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 297 329 669 701 709 741 811 843 1765
Size: 32 340 32 8 32 70 32 922
Cuts at: 1765 1795 1797 1827 297
Size: 30 2 30 2393
Fragments arranged by size:
2393 922 340 70 32 32 32 32
30 30 8 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 570 943 570
Size: 373 3550
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2682 2866 2682
Size: 184 3739
MscI TGG'CCA
Cuts at: 277 513 1684 277
Size: 236 1171 2516
Fragments arranged by size:
2516 1171 236
MslI CAynn'nnrTG
Cuts at: 460 1912 2039 460
Size: 1452 127 2344
Fragments arranged by size:
2344 1452 127
MspA1I CmG'CkG
Cuts at: 299 536 1034 1348 1708 2492 2737 299
Size: 237 498 314 360 784 245 1485
Fragments arranged by size:
1485 784 498 360 314 245 237
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 3923
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NcoI C'CATG_G
Cuts at: 107 107
Size: 3923
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 3923
NspI r_CATG'y
Cuts at: 976 1476 3079 976
Size: 500 1603 1820
Fragments arranged by size:
1820 1603 500
PciI A'CATG_T
Cuts at: 3075 3075
Size: 3923
PflMI CCAn_nnn'nTGG
Cuts at: 441 441
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2369 2401 2369
Size: 32 3891
PpuMI rG'GwC_Cy
Cuts at: 212 696 212
Size: 484 3439
PshAI GACnn'nnGTC
Cuts at: 793 793
Size: 3923
PspOMI G'GGCC_C
Cuts at: 134 134
Size: 3923
PstI C_TGCA'G
Cuts at: 304 779 970 1655 304
Size: 475 191 685 2572
Fragments arranged by size:
2572 685 475 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 299 1348 1708 299
Size: 1049 360 2514
Fragments arranged by size:
2514 1049 360
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SanDI GG'GwC_CC
Cuts at: 212 212
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SexAI A'CCwGG_T
Cuts at: 899 899
Size: 3923
SfcI C'TryA_G
Cuts at: 33 300 775 966 1651 2619 2810 33
Size: 267 475 191 685 968 191 1146
Fragments arranged by size:
1146 968 685 475 267 191 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 274 274
Size: 3923
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 210 945 210
Size: 735 3188
SmlI C'TyrA_G
Cuts at: 1078 1993 2707 2969 1078
Size: 915 714 262 2032
Fragments arranged by size:
2032 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StuI AGG'CCT
Cuts at: 146 146
Size: 3923
StyI C'CwwG_G
Cuts at: 107 189 1057 1411 107
Size: 82 868 354 2619
Fragments arranged by size:
2619 868 354 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 805 846 1506 1639 2098 2307 805
Size: 41 660 133 459 209 2421
Fragments arranged by size:
2421 660 459 209 133 41
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 224 256 581 613 3440 3472 224
Size: 32 325 32 2827 32 675
Fragments arranged by size:
2827 675 325 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 208 943 208
Size: 735 3188
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BspEI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PsiI PspXI PsrI SacII SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI