Restriction map for
pF1KE9545
(Circular) (Six-base) MAPSORT of: pF1KE9545.seq Check: 2897 from: 1 to: 6347
pF1KE9545 6347 bp
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1524 1795 2455 1524
Size: 271 660 5416
Fragments arranged by size:
5416 660 271
AccI GT'mk_AC
Cuts at: 1379 3385 1379
Size: 2006 4341
Acc65I G'GTAC_C
Cuts at: 3363 3363
Size: 6347
AclI AA'CG_TT
Cuts at: 6187 6187
Size: 6347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 807 1093 1129 1510 2517 3269 4172 4604 5351
Size: 286 36 381 1007 752 903 432 747
Cuts at: 5351 5619 807
Size: 268 1535
Fragments arranged by size:
1535 1007 903 752 747 432 381 286
268 36
AflIII A'CryG_T
Cuts at: 3699 4803 5780 3699
Size: 1104 977 4266
Fragments arranged by size:
4266 1104 977
AgeI A'CCGG_T
Cuts at: 4735 4735
Size: 6347
AhdI GACnn_n'nnGTC
Cuts at: 1875 1875
Size: 6347
AleI CACnn'nnGTG
Cuts at: 562 562
Size: 6347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 176 2323 176
Size: 2147 4200
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1879 1911 1879
Size: 32 6315
AlwNI CAG_nnn'CTG
Cuts at: 1488 1850 2283 2373 2913 2949 3652 5219 1488
Size: 362 433 90 540 36 703 1567 2616
Fragments arranged by size:
2616 1567 703 540 433 362 90 36
ApaLI G'TGCA_C
Cuts at: 5117 5117
Size: 6347
ApoI r'AATT_y
Cuts at: 781 888 1102 3078 3336 3351 5700 5956 781
Size: 107 214 1976 258 15 2349 256 1172
Fragments arranged by size:
2349 1976 1172 258 256 214 107 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 6347
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6347
AvaI C'yCGr_G
Cuts at: 2762 2887 3367 2762
Size: 125 480 5742
Fragments arranged by size:
5742 480 125
BamHI G'GATC_C
Cuts at: 2827 3372 2827
Size: 545 5802
BanI G'GyrC_C
Cuts at: 1054 1589 1716 3363 3777 4025 4060 1054
Size: 535 127 1647 414 248 35 3341
Fragments arranged by size:
3341 1647 535 414 248 127 35
BanII G_rGCy'C
Cuts at: 186 218 584 941 1436 1784 1940 2057 2123
Size: 32 366 357 495 348 156 117 66
Cuts at: 2123 2270 2413 3361 4391 186
Size: 147 143 948 1030 2142
Fragments arranged by size:
2142 1030 948 495 366 357 348 156
147 143 117 66 32
BbeI G_GCGC'C
Cuts at: 3781 4029 3781
Size: 248 6099
BbsI GAAGACnn'nnnn_
Cuts at: 2876 2876
Size: 6347
BbvCI CC'TCA_GC
Cuts at: 628 797 628
Size: 169 6178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2151 2185 2694 2728 2151
Size: 34 509 34 5770
Fragments arranged by size:
5770 509 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 382 4245 5006 6347 382
Size: 3863 761 1341 382
Fragments arranged by size:
3863 1341 761 382
BclI T'GATC_A
Cuts at: 3867 3867
Size: 6347
BfrBI ATG'CAT
Cuts at: 2189 2189
Size: 6347
BglI GCCn_nnn'nGGC
Cuts at: 997 2408 2632 2816 2892 997
Size: 1411 224 184 76 4452
Fragments arranged by size:
4452 1411 224 184 76
BglII A'GATC_T
Cuts at: 255 930 980 2675 3177 3862 5899 255
Size: 675 50 1695 502 685 2037 703
Fragments arranged by size:
2037 1695 703 685 675 502 50
BlpI GC'TnA_GC
Cuts at: 3459 3459
Size: 6347
Bme1580I G_kGCm'C
Cuts at: 739 1721 1864 3972 4065 5121 739
Size: 982 143 2108 93 1056 1965
Fragments arranged by size:
2108 1965 1056 982 143 93
BmgBI CAC'GTC
Cuts at: 2135 2135
Size: 6347
BmrI ACTGGGnnnn_n'
Cuts at: 738 834 3559 3640 3975 6119 738
Size: 96 2725 81 335 2144 966
Fragments arranged by size:
2725 2144 966 335 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1626 1684 4714 1626
Size: 58 3030 3259
Fragments arranged by size:
3259 3030 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 903 1692 2079 2395 3121 600
Size: 303 789 387 316 726 3826
Fragments arranged by size:
3826 789 726 387 316 303
Bpu10I CC'TnA_GC
Cuts at: 546 628 797 546
Size: 82 169 6096
Fragments arranged by size:
6096 169 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 2337 3523 4402 4894 5192 955
Size: 1382 1186 879 492 298 2110
Fragments arranged by size:
2110 1382 1186 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1315 3215 6051 1315
Size: 1900 2836 1611
Fragments arranged by size:
2836 1900 1611
BsaAI yAC'GTr
Cuts at: 1044 2399 3700 1044
Size: 1355 1301 3691
Fragments arranged by size:
3691 1355 1301
BsaBI GATnn'nnATC
Cuts at: 6 254 2317 2626 2680 3861 5898 6
Size: 248 2063 309 54 1181 2037 455
Fragments arranged by size:
2063 2037 1181 455 309 248 54
BsaHI Gr'CG_yC
Cuts at: 551 3778 4026 4728 6224 551
Size: 3227 248 702 1496 674
Fragments arranged by size:
3227 1496 702 674 248
BsaWI w'CCGG_w
Cuts at: 3536 4057 4735 5009 5156 5567 3536
Size: 521 678 274 147 411 4316
Fragments arranged by size:
4316 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1879 1903 1909 1933 2395 2425 3079 3109 1879
Size: 24 6 24 462 30 654 30 5117
Fragments arranged by size:
5117 654 462 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 603 616 1002 1696 2056 2059 2731 2829
Size: 245 13 386 694 360 3 672 98
Cuts at: 2829 2832 358
Size: 3 3873
Fragments arranged by size:
3873 694 672 386 360 245 98 13
3 3
BseYI C'CCAG_C
Cuts at: 128 360 384 576 1078 1971 1998 2924 3261
Size: 232 24 192 502 893 27 926 337
Cuts at: 3261 3772 4530 5107 5603 128
Size: 511 758 577 496 872
Fragments arranged by size:
926 893 872 758 577 511 502 496
337 232 192 27 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 2283 1475
Size: 808 5539
BsiEI CG_ry'CG
Cuts at: 104 769 3889 3935 5143 104
Size: 665 3120 46 1208 1308
Fragments arranged by size:
3120 1308 1208 665 46
BsiHKAI G_wGCw'C
Cuts at: 509 683 2057 2177 3361 4139 4329 5121 509
Size: 174 1374 120 1184 778 190 792 1735
Fragments arranged by size:
1735 1374 1184 792 778 190 174 120
BsmBI CGTCTCn'nnnn_
Cuts at: 558 2793 558
Size: 2235 4112
Bsp1286I G_dGCh'C
Cuts at: 186 218 509 584 683 739 941 1436 1721
Size: 32 291 75 99 56 202 495 285
Cuts at: 1721 1784 1864 1940 2057 2123 2177 2270 2413
Size: 63 80 76 117 66 54 93 143
Cuts at: 2413 3361 3972 4065 4139 4329 4391 5121 186
Size: 948 611 93 74 190 62 730 1412
Fragments arranged by size:
1412 948 730 611 495 291 285 202
190 143 117 99 93 93 80 76
75 74 66 63 62 56 54 32
BspEI T'CCGG_A
Cuts at: 5567 5567
Size: 6347
BspHI T'CATG_A
Cuts at: 5523 5523
Size: 6347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1423 1473 1524 1795 2282 2455 3397 3611 3913
Size: 50 51 271 487 173 942 214 302
Cuts at: 3913 4294 1423
Size: 381 3476
Fragments arranged by size:
3476 942 487 381 302 271 214 173
51 50
BsrBI CCG'CTC
Cuts at: 50 4639 4693 6177 6342 50
Size: 4589 54 1484 165 55
Fragments arranged by size:
4589 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1385 1901 4259 1385
Size: 516 2358 3473
Fragments arranged by size:
3473 2358 516
BsrFI r'CCGG_y
Cuts at: 2151 4345 4526 4735 2151
Size: 2194 181 209 3763
Fragments arranged by size:
3763 2194 209 181
BsrGI T'GTAC_A
Cuts at: 1291 1291
Size: 6347
BssSI C'ACGA_G
Cuts at: 2404 4618 4976 2404
Size: 2214 358 3775
Fragments arranged by size:
3775 2214 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2186 2283 3724 2186
Size: 97 1441 4809
Fragments arranged by size:
4809 1441 97
BstBI TT'CG_AA
Cuts at: 4708 4708
Size: 6347
BstEII G'GTnAC_C
Cuts at: 1536 1555 3024 1536
Size: 19 1469 4859
Fragments arranged by size:
4859 1469 19
BstXI CCAn_nnnn'nTGG
Cuts at: 564 696 1048 564
Size: 132 352 5863
Fragments arranged by size:
5863 352 132
BstYI r'GATC_y
Cuts at: 1 255 860 930 980 1085 2234 2561 2675
Size: 254 605 70 50 105 1149 327 114
Cuts at: 2675 2827 3177 3372 3839 3862 4197 4443 5455
Size: 152 350 195 467 23 335 246 1012
Cuts at: 5455 5541 5553 5899 1
Size: 86 12 346 449
Fragments arranged by size:
1149 1012 605 467 449 350 346 335
327 254 246 195 152 114 105 86
70 50 23 12
BtgI C'CryG_G
Cuts at: 107 303 557 695 893 2822 3065 107
Size: 196 254 138 198 1929 243 3389
Fragments arranged by size:
3389 1929 254 243 198 196 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4297 4478 4641 4297
Size: 181 163 6003
Fragments arranged by size:
6003 181 163
BtsI GCAGTG_nn'
Cuts at: 569 689 1320 3163 3590 569
Size: 120 631 1843 427 3326
Fragments arranged by size:
3326 1843 631 427 120
DraI TTT'AAA
Cuts at: 3346 3346
Size: 6347
DrdI GACnn_nn'nnGTC
Cuts at: 4053 4911 4053
Size: 858 5489
EaeI y'GGCC_r
Cuts at: 766 896 989 3229 3932 4106 4497 4524 5869
Size: 130 93 2240 703 174 391 27 1345
Cuts at: 5869 766
Size: 1244
Fragments arranged by size:
2240 1345 1244 703 391 174 130 93
27
EagI C'GGCC_G
Cuts at: 766 3932 766
Size: 3166 3181
EarI CTCTTCn'nnn_
Cuts at: 589 851 1441 1675 2077 2695 3245 4370 4580
Size: 262 590 234 402 618 550 1125 210
Cuts at: 4580 589
Size: 2356
Fragments arranged by size:
2356 1125 618 590 550 402 262 234
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4555 4865 5011 6157 4555
Size: 310 146 1146 4745
Fragments arranged by size:
4745 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2055 3359 2055
Size: 1304 5043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 807 903 1093 1129 1510 1692 2079 2395
Size: 207 96 190 36 381 182 387 316
Cuts at: 2395 2517 3121 3269 4172 4604 5351 5619 600
Size: 122 604 148 903 432 747 268 1328
Fragments arranged by size:
1328 903 747 604 432 387 381 316
268 207 190 182 148 122 96 36
EcoNI CCTnn'n_nnAGG
Cuts at: 946 1111 1427 946
Size: 165 316 5866
Fragments arranged by size:
5866 316 165
EcoO109I rG'GnC_Cy
Cuts at: 132 1204 1485 3208 3243 3486 132
Size: 1072 281 1723 35 243 2993
Fragments arranged by size:
2993 1723 1072 281 243 35
EcoRI G'AATT_C
Cuts at: 1102 3078 3336 3351 1102
Size: 1976 258 15 4098
Fragments arranged by size:
4098 1976 258 15
EcoRV GAT'ATC
Cuts at: 810 1355 810
Size: 545 5802
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3024 4366 3024
Size: 1342 5005
FspI TGC'GCA
Cuts at: 4128 4128
Size: 6347
HaeII r_GCGC'y
Cuts at: 3781 4029 5051 6030 3781
Size: 248 1022 979 4098
Fragments arranged by size:
4098 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 1658 1690 1861 1893 1909 1941 4189
Size: 32 1379 32 171 32 16 32 2248
Cuts at: 4189 4219 4221 4251 247
Size: 30 2 30 2343
Fragments arranged by size:
2343 2248 1379 171 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 3386 3386
Size: 6347
KasI G'GCGC_C
Cuts at: 3777 4025 3777
Size: 248 6099
KpnI G_GTAC'C
Cuts at: 3367 3367
Size: 6347
MfeI C'AATT_G
Cuts at: 740 740
Size: 6347
MluI A'CGCG_T
Cuts at: 5780 5780
Size: 6347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1751 5018 5202 1305
Size: 446 3267 184 2450
Fragments arranged by size:
3267 2450 446 184
MscI TGG'CCA
Cuts at: 991 4108 991
Size: 3117 3230
MslI CAynn'nnrTG
Cuts at: 562 694 2194 2305 2323 4336 4463 562
Size: 132 1500 111 18 2013 127 2446
Fragments arranged by size:
2446 2013 1500 132 127 111 18
MspA1I CmG'CkG
Cuts at: 722 748 2913 3017 3153 3228 3458 3772 4132
Size: 26 2165 104 136 75 230 314 360
Cuts at: 4132 5145 5390 722
Size: 1013 245 1679
Fragments arranged by size:
2165 1679 1013 360 314 245 230 136
104 75 26
NaeI GCC'GGC
Cuts at: 2153 4528 2153
Size: 2375 3972
NarI GG'CG_CC
Cuts at: 3778 4026 3778
Size: 248 6099
NcoI C'CATG_G
Cuts at: 107 695 3065 107
Size: 588 2370 3389
Fragments arranged by size:
3389 2370 588
NgoMIV G'CCGG_C
Cuts at: 2151 4526 2151
Size: 2375 3972
NsiI A_TGCA'T
Cuts at: 2191 2191
Size: 6347
NspI r_CATG'y
Cuts at: 871 2541 3400 3900 4807 871
Size: 1670 859 500 907 2411
Fragments arranged by size:
2411 1670 907 859 500
PasI CC'CwG_GG
Cuts at: 2424 2424
Size: 6347
PciI A'CATG_T
Cuts at: 4803 4803
Size: 6347
PflMI CCAn_nnn'nTGG
Cuts at: 870 1606 2373 870
Size: 736 767 4844
Fragments arranged by size:
4844 767 736
PfoI T'CCnGG_A
Cuts at: 1097 2488 3188 1097
Size: 1391 700 4256
Fragments arranged by size:
4256 1391 700
PmeI GTTT'AAAC
Cuts at: 3346 3346
Size: 6347
PmlI CAC'GTG
Cuts at: 1044 2399 1044
Size: 1355 4992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1879 1911 5486 5518 1879
Size: 32 3575 32 2708
Fragments arranged by size:
3575 2708 32 32
PpuMI rG'GwC_Cy
Cuts at: 132 1204 1485 3208 3243 132
Size: 1072 281 1723 35 3236
Fragments arranged by size:
3236 1723 1072 281 35
PstI C_TGCA'G
Cuts at: 1735 2279 3158 3394 4079 1735
Size: 544 879 236 685 4003
Fragments arranged by size:
4003 879 685 544 236
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6347
PvuII CAG'CTG
Cuts at: 748 3772 4132 748
Size: 3024 360 2963
Fragments arranged by size:
3024 2963 360
RsrII CG'GwC_CG
Cuts at: 4542 4542
Size: 6347
SacI G_AGCT'C
Cuts at: 2057 3361 2057
Size: 1304 5043
SalI G'TCGA_C
Cuts at: 3384 3384
Size: 6347
SapI GCTCTTCn'nnn_
Cuts at: 589 1441 4370 4580 589
Size: 852 2929 210 2356
Fragments arranged by size:
2929 2356 852 210
SbfI CC_TGCA'GG
Cuts at: 3394 3394
Size: 6347
ScaI AGT'ACT
Cuts at: 844 1036 5864 844
Size: 192 4828 1327
Fragments arranged by size:
4828 1327 192
SexAI A'CCwGG_T
Cuts at: 1559 1559
Size: 6347
SfcI C'TryA_G
Cuts at: 33 1731 2275 3154 3390 4075 5068 5259 33
Size: 1698 544 879 236 685 993 191 1121
Fragments arranged by size:
1698 1121 993 879 685 544 236 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 997 997
Size: 6347
SfoI GGC'GCC
Cuts at: 3779 4027 3779
Size: 248 6099
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6347
SmaI CCC'GGG
Cuts at: 2764 3369 2764
Size: 605 5742
SmlI C'TyrA_G
Cuts at: 934 2352 3502 4417 4909 5171 934
Size: 1418 1150 915 492 262 2110
Fragments arranged by size:
2110 1418 1150 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6347
SphI G_CATG'C
Cuts at: 2541 3400 3900 2541
Size: 859 500 4988
Fragments arranged by size:
4988 859 500
SrfI GCCC'GGGC
Cuts at: 2764 2764
Size: 6347
StuI AGG'CCT
Cuts at: 427 1000 427
Size: 573 5774
StyI C'CwwG_G
Cuts at: 107 207 428 695 1151 1907 2811 3065 3481
Size: 100 221 267 456 756 904 254 416
Cuts at: 3481 3835 107
Size: 354 2619
Fragments arranged by size:
2619 904 756 456 416 354 267 254
221 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 720 1990 2043 3205 3930 4063 4522 4731 720
Size: 1270 53 1162 725 133 459 209 2336
Fragments arranged by size:
2336 1270 1162 725 459 209 133 53
TatI w'GTAC_w
Cuts at: 842 1034 1291 1367 5862 842
Size: 192 257 76 4495 1327
Fragments arranged by size:
4495 1327 257 192 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2119 2151 2395 2427 3079 3111 5864 5896 2119
Size: 32 244 32 652 32 2753 32 2570
Fragments arranged by size:
2753 2570 652 244 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4144 4144
Size: 6347
XbaI T'CTAG_A
Cuts at: 3378 3378
Size: 6347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 906 1871 2155 3842 381
Size: 525 965 284 1687 2886
Fragments arranged by size:
2886 1687 965 525 284
XmaI C'CCGG_G
Cuts at: 2762 3367 2762
Size: 605 5742
XmnI GAAnn'nnTTC
Cuts at: 643 643
Size: 6347
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII ApaI AscI AvrII BaeI BmtI
BsiWI BsmI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
PacI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SgrAI SnaBI SspI SwaI XhoI ZraI