Restriction map for
pF1KE9527
(Circular) (Six-base) MAPSORT of: pF1KE9527.seq Check: 2931 from: 1 to: 4304
pF1KE9527 4304 bp
With 182 enzymes: SgfI *
August 25, 2016 10:58 ..
AccI GT'mk_AC
Cuts at: 350 1342 350
Size: 992 3312
Acc65I G'GTAC_C
Cuts at: 1320 1320
Size: 4304
AclI AA'CG_TT
Cuts at: 4144 4144
Size: 4304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2561 3308 3576 2129
Size: 432 747 268 2857
Fragments arranged by size:
2857 747 432 268
AflIII A'CryG_T
Cuts at: 581 821 1656 2760 3737 581
Size: 240 835 1104 977 1148
Fragments arranged by size:
1148 1104 977 835 240
AgeI A'CCGG_T
Cuts at: 2692 2692
Size: 4304
AleI CACnn'nnGTG
Cuts at: 358 358
Size: 4304
AlwNI CAG_nnn'CTG
Cuts at: 886 1609 3176 886
Size: 723 1567 2014
Fragments arranged by size:
2014 1567 723
ApaLI G'TGCA_C
Cuts at: 3074 3074
Size: 4304
ApoI r'AATT_y
Cuts at: 1308 3657 3913 1308
Size: 2349 256 1699
Fragments arranged by size:
2349 1699 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4304
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4304
AvaI C'yCGr_G
Cuts at: 1324 1324
Size: 4304
BamHI G'GATC_C
Cuts at: 1329 1329
Size: 4304
BanI G'GyrC_C
Cuts at: 178 1320 1734 1982 2017 178
Size: 1142 414 248 35 2465
Fragments arranged by size:
2465 1142 414 248 35
BanII G_rGCy'C
Cuts at: 118 1318 2348 118
Size: 1200 1030 2074
Fragments arranged by size:
2074 1200 1030
BbeI G_GCGC'C
Cuts at: 1738 1986 1738
Size: 248 4056
BbsI GAAGACnn'nnnn_
Cuts at: 327 327
Size: 4304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4270
BciVI GTATCCnnnnn_n'
Cuts at: 1233 2202 2963 4304 1233
Size: 969 761 1341 1233
Fragments arranged by size:
1341 1233 969 761
BclI T'GATC_A
Cuts at: 902 1824 902
Size: 922 3382
BglI GCCn_nnn'nGGC
Cuts at: 457 457
Size: 4304
BglII A'GATC_T
Cuts at: 1819 3856 1819
Size: 2037 2267
BlpI GC'TnA_GC
Cuts at: 1416 1416
Size: 4304
Bme1580I G_kGCm'C
Cuts at: 656 1929 2022 3078 656
Size: 1273 93 1056 1882
Fragments arranged by size:
1882 1273 1056 93
BmgBI CAC'GTC
Cuts at: 249 249
Size: 4304
BmrI ACTGGGnnnn_n'
Cuts at: 1516 1597 1932 4076 1516
Size: 81 335 2144 1744
Fragments arranged by size:
2144 1744 335 81
BmtI G_CTAG'C
Cuts at: 157 157
Size: 4304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 4304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 1230 642
Size: 588 3716
Bpu10I CC'TnA_GC
Cuts at: 800 800
Size: 4304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1480 2359 2851 3149 1480
Size: 879 492 298 2635
Fragments arranged by size:
2635 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4008 4008
Size: 4304
BsaAI yAC'GTr
Cuts at: 1657 1657
Size: 4304
BsaBI GATnn'nnATC
Cuts at: 6 1818 3855 6
Size: 1812 2037 455
Fragments arranged by size:
2037 1812 455
BsaHI Gr'CG_yC
Cuts at: 1735 1983 2685 4181 1735
Size: 248 702 1496 1858
Fragments arranged by size:
1858 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1493 2014 2692 2966 3113 3524 1493
Size: 521 678 274 147 411 2273
Fragments arranged by size:
2273 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 254 284 254
Size: 30 4274
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 463 463
Size: 4304
BseYI C'CCAG_C
Cuts at: 118 135 867 1729 2487 3064 3560 118
Size: 17 732 862 758 577 496 862
Fragments arranged by size:
862 862 758 732 577 496 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 640
Size: 4304
BsiEI CG_ry'CG
Cuts at: 104 1846 1892 3100 104
Size: 1742 46 1208 1308
Fragments arranged by size:
1742 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1318 2096 2286 3078 1318
Size: 778 190 792 2544
Fragments arranged by size:
2544 792 778 190
BsmI GAATG_Cn'
Cuts at: 914 914
Size: 4304
Bsp1286I G_dGCh'C
Cuts at: 118 656 1318 1929 2022 2096 2286 2348 3078
Size: 538 662 611 93 74 190 62 730
Cuts at: 3078 118
Size: 1344
Fragments arranged by size:
1344 730 662 611 538 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3524 3524
Size: 4304
BspHI T'CATG_A
Cuts at: 602 786 3480 602
Size: 184 2694 1426
Fragments arranged by size:
2694 1426 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 616 1354 1568 1870 2251 616
Size: 738 214 302 381 2669
Fragments arranged by size:
2669 738 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2596 2650 4134 4299 50
Size: 2546 54 1484 165 55
Fragments arranged by size:
2546 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 674 2216 674
Size: 1542 2762
BsrFI r'CCGG_y
Cuts at: 2302 2483 2692 2302
Size: 181 209 3914
Fragments arranged by size:
3914 209 181
BsrGI T'GTAC_A
Cuts at: 1082 1082
Size: 4304
BssSI C'ACGA_G
Cuts at: 2575 2933 2575
Size: 358 3946
BstAPI GCAn_nnn'nTGC
Cuts at: 676 1681 676
Size: 1005 3299
BstBI TT'CG_AA
Cuts at: 2665 2665
Size: 4304
BstEII G'GTnAC_C
Cuts at: 720 720
Size: 4304
BstXI CCAn_nnnn'nTGG
Cuts at: 253 253
Size: 4304
BstYI r'GATC_y
Cuts at: 1 199 1329 1796 1819 2154 2400 3412 3498
Size: 198 1130 467 23 335 246 1012 86
Cuts at: 3498 3510 3856 1
Size: 12 346 449
Fragments arranged by size:
1130 1012 467 449 346 335 246 198
86 23 12
BtgI C'CryG_G
Cuts at: 647 926 647
Size: 279 4025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2254 2435 2598 2254
Size: 181 163 3960
Fragments arranged by size:
3960 181 163
BtsI GCAGTG_nn'
Cuts at: 180 1547 180
Size: 1367 2937
DraI TTT'AAA
Cuts at: 1303 1303
Size: 4304
DrdI GACnn_nn'nnGTC
Cuts at: 1132 2010 2868 1132
Size: 878 858 2568
Fragments arranged by size:
2568 878 858
EaeI y'GGCC_r
Cuts at: 449 845 986 1889 2063 2454 2481 3826 449
Size: 396 141 903 174 391 27 1345 927
Fragments arranged by size:
1345 927 903 396 391 174 141 27
EagI C'GGCC_G
Cuts at: 1889 1889
Size: 4304
EarI CTCTTCn'nnn_
Cuts at: 604 949 1272 2327 2537 604
Size: 345 323 1055 210 2371
Fragments arranged by size:
2371 1055 345 323 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2512 2822 2968 4114 2512
Size: 310 146 1146 2702
Fragments arranged by size:
2702 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1316 1316
Size: 4304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 1230 2129 2561 3308 3576 642
Size: 588 899 432 747 268 1370
Fragments arranged by size:
1370 899 747 588 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1443 1443
Size: 4304
EcoRI G'AATT_C
Cuts at: 1308 1308
Size: 4304
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 4304
FspI TGC'GCA
Cuts at: 2085 2085
Size: 4304
HaeII r_GCGC'y
Cuts at: 1738 1986 3008 3987 1738
Size: 248 1022 979 2055
Fragments arranged by size:
2055 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 226 258 460 492 524 922 954 2146 2176
Size: 32 202 32 32 398 32 1192 30
Cuts at: 2176 2178 2208 226
Size: 2 30 2322
Fragments arranged by size:
2322 1192 398 202 32 32 32 32
30 30 2
HincII GTy'rAC
Cuts at: 1343 1343
Size: 4304
KasI G'GCGC_C
Cuts at: 1734 1982 1734
Size: 248 4056
KpnI G_GTAC'C
Cuts at: 1324 1324
Size: 4304
MfeI C'AATT_G
Cuts at: 344 356 344
Size: 12 4292
MluI A'CGCG_T
Cuts at: 3737 3737
Size: 4304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2975 3159 2975
Size: 184 4120
MscI TGG'CCA
Cuts at: 451 847 988 2065 451
Size: 396 141 1077 2690
Fragments arranged by size:
2690 1077 396 141
MslI CAynn'nnrTG
Cuts at: 358 515 1073 2293 2420 358
Size: 157 558 1220 127 2242
Fragments arranged by size:
2242 1220 558 157 127
MspA1I CmG'CkG
Cuts at: 1118 1415 1729 2089 3102 3347 1118
Size: 297 314 360 1013 245 2075
Fragments arranged by size:
2075 1013 360 314 297 245
NaeI GCC'GGC
Cuts at: 2485 2485
Size: 4304
NarI GG'CG_CC
Cuts at: 1735 1983 1735
Size: 248 4056
NcoI C'CATG_G
Cuts at: 647 926 647
Size: 279 4025
NgoMIV G'CCGG_C
Cuts at: 2483 2483
Size: 4304
NheI G'CTAG_C
Cuts at: 153 153
Size: 4304
NspI r_CATG'y
Cuts at: 585 825 1357 1857 2764 585
Size: 240 532 500 907 2125
Fragments arranged by size:
2125 907 532 500 240
PciI A'CATG_T
Cuts at: 581 821 2760 581
Size: 240 1939 2125
Fragments arranged by size:
2125 1939 240
PfoI T'CCnGG_A
Cuts at: 658 1246 658
Size: 588 3716
PmeI GTTT'AAAC
Cuts at: 1303 1303
Size: 4304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 4272
PshAI GACnn'nnGTC
Cuts at: 331 331
Size: 4304
PstI C_TGCA'G
Cuts at: 175 1351 2036 175
Size: 1176 685 2443
Fragments arranged by size:
2443 1176 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4304
PvuII CAG'CTG
Cuts at: 1118 1729 2089 1118
Size: 611 360 3333
Fragments arranged by size:
3333 611 360
RsrII CG'GwC_CG
Cuts at: 2499 2499
Size: 4304
SacI G_AGCT'C
Cuts at: 1318 1318
Size: 4304
SalI G'TCGA_C
Cuts at: 1341 1341
Size: 4304
SapI GCTCTTCn'nnn_
Cuts at: 2327 2537 2327
Size: 210 4094
SbfI CC_TGCA'GG
Cuts at: 1351 1351
Size: 4304
ScaI AGT'ACT
Cuts at: 3821 3821
Size: 4304
SfcI C'TryA_G
Cuts at: 33 171 1347 2032 3025 3216 33
Size: 138 1176 685 993 191 1121
Fragments arranged by size:
1176 1121 993 685 191 138
SfiI GGCCn_nnn'nGGCC
Cuts at: 457 457
Size: 4304
SfoI GGC'GCC
Cuts at: 1736 1984 1736
Size: 248 4056
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4304
SmaI CCC'GGG
Cuts at: 1326 1326
Size: 4304
SmlI C'TyrA_G
Cuts at: 1459 2374 2866 3128 1459
Size: 915 492 262 2635
Fragments arranged by size:
2635 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4304
SphI G_CATG'C
Cuts at: 1357 1857 1357
Size: 500 3804
StyI C'CwwG_G
Cuts at: 186 461 647 926 1438 1792 186
Size: 275 186 279 512 354 2698
Fragments arranged by size:
2698 512 354 279 275 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1887 2020 2479 2688 1887
Size: 133 459 209 3503
Fragments arranged by size:
3503 459 209 133
TatI w'GTAC_w
Cuts at: 584 1082 3819 584
Size: 498 2737 1069
Fragments arranged by size:
2737 1069 498
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3821 3853 3821
Size: 32 4272
Tth111I GACn'n_nGTC
Cuts at: 2101 2101
Size: 4304
XbaI T'CTAG_A
Cuts at: 1335 1335
Size: 4304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 1799 459
Size: 1340 2964
XmaI C'CCGG_G
Cuts at: 1324 1324
Size: 4304
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI