Restriction map for
pF1KE9522
(Circular) (Six-base) MAPSORT of: pF1KE9522.seq Check: 3159 from: 1 to: 5729
pF1KE9522 5729 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 347 2422 347
Size: 2075 3654
AccI GT'mk_AC
Cuts at: 2767 2767
Size: 5729
Acc65I G'GTAC_C
Cuts at: 2745 2745
Size: 5729
AclI AA'CG_TT
Cuts at: 5569 5569
Size: 5729
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3554 3986 4733 5001 3554
Size: 432 747 268 4282
Fragments arranged by size:
4282 747 432 268
AflIII A'CryG_T
Cuts at: 3081 4185 5162 3081
Size: 1104 977 3648
Fragments arranged by size:
3648 1104 977
AgeI A'CCGG_T
Cuts at: 4117 4117
Size: 5729
AleI CACnn'nnGTG
Cuts at: 979 1894 1996 979
Size: 915 102 4712
Fragments arranged by size:
4712 915 102
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1147 1179 1147
Size: 32 5697
AlwNI CAG_nnn'CTG
Cuts at: 291 414 1107 3034 4601 291
Size: 123 693 1927 1567 1419
Fragments arranged by size:
1927 1567 1419 693 123
ApaI G_GGCC'C
Cuts at: 169 1005 2100 169
Size: 836 1095 3798
Fragments arranged by size:
3798 1095 836
ApaLI G'TGCA_C
Cuts at: 226 362 4499 226
Size: 136 4137 1456
Fragments arranged by size:
4137 1456 136
ApoI r'AATT_y
Cuts at: 1147 1708 2733 5082 5338 1147
Size: 561 1025 2349 256 1538
Fragments arranged by size:
2349 1538 1025 561 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5729
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5729
AvaI C'yCGr_G
Cuts at: 932 2099 2169 2749 932
Size: 1167 70 580 3912
Fragments arranged by size:
3912 1167 580 70
BamHI G'GATC_C
Cuts at: 2754 2754
Size: 5729
BanI G'GyrC_C
Cuts at: 1352 1840 2107 2211 2595 2745 3159 3407 3442
Size: 488 267 104 384 150 414 248 35
Cuts at: 3442 1352
Size: 3639
Fragments arranged by size:
3639 488 414 384 267 248 150 104
35
BanII G_rGCy'C
Cuts at: 169 876 1005 1616 1922 2100 2299 2743 3773
Size: 707 129 611 306 178 199 444 1030
Cuts at: 3773 169
Size: 2125
Fragments arranged by size:
2125 1030 707 611 444 306 199 178
129
BbeI G_GCGC'C
Cuts at: 1356 3163 3411 1356
Size: 1807 248 3674
Fragments arranged by size:
3674 1807 248
BbsI GAAGACnn'nnnn_
Cuts at: 1938 1938
Size: 5729
BbvCI CC'TCA_GC
Cuts at: 161 1040 1578 2506 161
Size: 879 538 928 3384
Fragments arranged by size:
3384 928 879 538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2150 2184 2150
Size: 34 5695
BciVI GTATCCnnnnn_n'
Cuts at: 3627 4388 5729 3627
Size: 761 1341 3627
Fragments arranged by size:
3627 1341 761
BclI T'GATC_A
Cuts at: 3249 3249
Size: 5729
BglI GCCn_nnn'nGGC
Cuts at: 476 901 1760 2510 2612 476
Size: 425 859 750 102 3593
Fragments arranged by size:
3593 859 750 425 102
BglII A'GATC_T
Cuts at: 3244 5281 3244
Size: 2037 3692
BlpI GC'TnA_GC
Cuts at: 2841 2841
Size: 5729
Bme1580I G_kGCm'C
Cuts at: 169 230 366 798 897 1005 1220 1302 1845
Size: 61 136 432 99 108 215 82 543
Cuts at: 1845 2029 2100 2112 2221 2365 2598 3354 3447
Size: 184 71 12 109 144 233 756 93
Cuts at: 3447 4503 169
Size: 1056 1395
Fragments arranged by size:
1395 1056 756 543 432 233 215 184
144 136 109 108 99 93 82 71
61 12
BmrI ACTGGGnnnn_n'
Cuts at: 217 884 1536 2658 2941 3022 3357 5501 217
Size: 667 652 1122 283 81 335 2144 445
Fragments arranged by size:
2144 1122 667 652 445 335 283 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1083 4096 1083
Size: 3013 2716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 313 424 1027 1606 2442 2622 211
Size: 102 111 603 579 836 180 3318
Fragments arranged by size:
3318 836 603 579 180 111 102
Bpu10I CC'TnA_GC
Cuts at: 161 1040 1578 2506 161
Size: 879 538 928 3384
Fragments arranged by size:
3384 928 879 538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 2905 3784 4276 4574 591
Size: 2314 879 492 298 1746
Fragments arranged by size:
2314 1746 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5433 5433
Size: 5729
BsaAI yAC'GTr
Cuts at: 3082 3082
Size: 5729
BsaBI GATnn'nnATC
Cuts at: 6 3243 5280 6
Size: 3237 2037 455
Fragments arranged by size:
3237 2037 455
BsaHI Gr'CG_yC
Cuts at: 739 1353 2013 3160 3408 4110 5606 739
Size: 614 660 1147 248 702 1496 862
Fragments arranged by size:
1496 1147 862 702 660 614 248
BsaWI w'CCGG_w
Cuts at: 2918 3439 4117 4391 4538 4949 2918
Size: 521 678 274 147 411 3698
Fragments arranged by size:
3698 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 188 218 367 397 648 678 1075 1105 1147
Size: 30 149 30 251 30 397 30 42
Cuts at: 1147 1177 188
Size: 30 4740
Fragments arranged by size:
4740 397 251 149 42 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2308 2308
Size: 5729
BseYI C'CCAG_C
Cuts at: 168 207 243 1407 1793 1863 1935 2111 3154
Size: 39 36 1164 386 70 72 176 1043
Cuts at: 3154 3912 4489 4985 168
Size: 758 577 496 912
Fragments arranged by size:
1164 1043 912 758 577 496 386 176
72 70 39 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1663 2316 2533 1508
Size: 155 653 217 4704
Fragments arranged by size:
4704 653 217 155
BsiEI CG_ry'CG
Cuts at: 104 3271 3317 4525 104
Size: 3167 46 1208 1308
Fragments arranged by size:
3167 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 230 275 366 1379 1922 2318 2743 3521 3711
Size: 45 91 1013 543 396 425 778 190
Cuts at: 3711 4503 230
Size: 792 1456
Fragments arranged by size:
1456 1013 792 778 543 425 396 190
91 45
BsmI GAATG_Cn'
Cuts at: 1673 2431 1673
Size: 758 4971
Bsp1286I G_dGCh'C
Cuts at: 169 230 275 366 798 876 897 1005 1220
Size: 61 45 91 432 78 21 108 215
Cuts at: 1220 1302 1379 1616 1845 1922 2029 2100 2112
Size: 82 77 237 229 77 107 71 12
Cuts at: 2112 2221 2299 2318 2365 2598 2743 3354 3447
Size: 109 78 19 47 233 145 611 93
Cuts at: 3447 3521 3711 3773 4503 169
Size: 74 190 62 730 1395
Fragments arranged by size:
1395 730 611 432 237 233 229 215
190 145 109 108 107 93 91 82
78 78 77 77 74 71 62 61
47 45 21 19 12
BspEI T'CCGG_A
Cuts at: 4949 4949
Size: 5729
BspHI T'CATG_A
Cuts at: 4905 4905
Size: 5729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 2422 2779 2993 3295 3676 347
Size: 2075 357 214 302 381 2400
Fragments arranged by size:
2400 2075 381 357 302 214
BsrBI CCG'CTC
Cuts at: 50 4021 4075 5559 5724 50
Size: 3971 54 1484 165 55
Fragments arranged by size:
3971 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 2075 2691 3641 1976
Size: 99 616 950 4064
Fragments arranged by size:
4064 950 616 99
BsrFI r'CCGG_y
Cuts at: 1582 3727 3908 4117 1582
Size: 2145 181 209 3194
Fragments arranged by size:
3194 2145 209 181
BsrGI T'GTAC_A
Cuts at: 2405 2405
Size: 5729
BssSI C'ACGA_G
Cuts at: 791 4000 4358 791
Size: 3209 358 2162
Fragments arranged by size:
3209 2162 358
BstAPI GCAn_nnn'nTGC
Cuts at: 342 1679 3106 342
Size: 1337 1427 2965
Fragments arranged by size:
2965 1427 1337
BstBI TT'CG_AA
Cuts at: 1769 4090 1769
Size: 2321 3408
BstXI CCAn_nnnn'nTGG
Cuts at: 558 690 1062 1896 2157 2656 558
Size: 132 372 834 261 499 3631
Fragments arranged by size:
3631 834 499 372 261 132
BstYI r'GATC_y
Cuts at: 1 584 2754 3221 3244 3579 3825 4837 4923
Size: 583 2170 467 23 335 246 1012 86
Cuts at: 4923 4935 5281 1
Size: 12 346 449
Fragments arranged by size:
2170 1012 583 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1908 1908
Size: 5729
BtgI C'CryG_G
Cuts at: 107 276 441 492 689 1268 1564 1991 2516
Size: 169 165 51 197 579 296 427 525
Cuts at: 2516 107
Size: 3320
Fragments arranged by size:
3320 579 525 427 296 197 169 165
51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1348 1812 1814 2294 2307 2702 3679 3860 4023
Size: 464 2 480 13 395 977 181 163
Cuts at: 4023 1348
Size: 3054
Fragments arranged by size:
3054 977 480 464 395 181 163 13
2
BtsI GCAGTG_nn'
Cuts at: 133 506 1032 1265 1500 1614 2003 2027 2260
Size: 373 526 233 235 114 389 24 233
Cuts at: 2260 2622 2972 133
Size: 362 350 2890
Fragments arranged by size:
2890 526 389 373 362 350 235 233
233 114 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1839 1874 1839
Size: 35 5694
DraI TTT'AAA
Cuts at: 2728 2728
Size: 5729
DraIII CAC_nnn'GTG
Cuts at: 2489 2489
Size: 5729
DrdI GACnn_nn'nnGTC
Cuts at: 726 3435 4293 726
Size: 2709 858 2162
Fragments arranged by size:
2709 2162 858
EaeI y'GGCC_r
Cuts at: 560 812 983 1339 1458 1847 2116 2147 2646
Size: 252 171 356 119 389 269 31 499
Cuts at: 2646 2688 3314 3488 3879 3906 5251 560
Size: 42 626 174 391 27 1345 1038
Fragments arranged by size:
1345 1038 626 499 391 389 356 269
252 174 171 119 42 31 27
EagI C'GGCC_G
Cuts at: 3314 3314
Size: 5729
EarI CTCTTCn'nnn_
Cuts at: 1630 2566 3752 3962 1630
Size: 936 1186 210 3397
Fragments arranged by size:
3397 1186 936 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3937 4247 4393 5539 3937
Size: 310 146 1146 4127
Fragments arranged by size:
4127 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1920 2741 1920
Size: 821 4908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 313 424 1027 1606 2442 2622 3554 3986
Size: 102 111 603 579 836 180 932 432
Cuts at: 3986 4733 5001 211
Size: 747 268 939
Fragments arranged by size:
939 932 836 747 603 579 432 268
180 111 102
EcoNI CCTnn'n_nnAGG
Cuts at: 1363 1363
Size: 5729
EcoO109I rG'GnC_Cy
Cuts at: 165 1001 1002 1816 2096 2868 165
Size: 836 1 814 280 772 3026
Fragments arranged by size:
3026 836 814 772 280 1
EcoRI G'AATT_C
Cuts at: 1147 1708 2733 1147
Size: 561 1025 4143
Fragments arranged by size:
4143 1025 561
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3748 3748
Size: 5729
FspI TGC'GCA
Cuts at: 1194 3510 1194
Size: 2316 3413
HaeII r_GCGC'y
Cuts at: 291 960 1356 1503 3163 3411 4433 5412 291
Size: 669 396 147 1660 248 1022 979 608
Fragments arranged by size:
1660 1022 979 669 608 396 248 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 1075 1107 1216 1248 3571 3601 3603
Size: 32 676 32 109 32 2323 30 2
Cuts at: 3603 3633 367
Size: 30 2463
Fragments arranged by size:
2463 2323 676 109 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1347 2768 1347
Size: 1421 4308
HpaI GTT'AAC
Cuts at: 1347 1347
Size: 5729
KasI G'GCGC_C
Cuts at: 1352 3159 3407 1352
Size: 1807 248 3674
Fragments arranged by size:
3674 1807 248
KpnI G_GTAC'C
Cuts at: 2749 2749
Size: 5729
MluI A'CGCG_T
Cuts at: 5162 5162
Size: 5729
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4400 4584 4400
Size: 184 5545
MscI TGG'CCA
Cuts at: 562 814 985 1849 2149 2648 3490 562
Size: 252 171 864 300 499 842 2801
Fragments arranged by size:
2801 864 842 499 300 252 171
MslI CAynn'nnrTG
Cuts at: 497 556 808 979 1246 1894 1996 2279 2485
Size: 59 252 171 267 648 102 283 206
Cuts at: 2485 2491 3718 3845 497
Size: 6 1227 127 2381
Fragments arranged by size:
2381 1227 648 283 267 252 206 171
127 102 59 6
MspA1I CmG'CkG
Cuts at: 1169 1411 1793 2115 2178 2510 2840 3154 3514
Size: 242 382 322 63 332 330 314 360
Cuts at: 3514 4527 4772 1169
Size: 1013 245 2126
Fragments arranged by size:
2126 1013 382 360 332 330 322 314
245 242 63
NaeI GCC'GGC
Cuts at: 1584 3910 1584
Size: 2326 3403
NarI GG'CG_CC
Cuts at: 1353 3160 3408 1353
Size: 1807 248 3674
Fragments arranged by size:
3674 1807 248
NcoI C'CATG_G
Cuts at: 107 492 689 1268 1564 107
Size: 385 197 579 296 4272
Fragments arranged by size:
4272 579 385 296 197
NgoMIV G'CCGG_C
Cuts at: 1582 3908 1582
Size: 2326 3403
NspI r_CATG'y
Cuts at: 400 1293 2782 3282 4189 400
Size: 893 1489 500 907 1940
Fragments arranged by size:
1940 1489 907 893 500
PciI A'CATG_T
Cuts at: 4185 4185
Size: 5729
PflMI CCAn_nnn'nTGG
Cuts at: 291 1107 1518 291
Size: 816 411 4502
Fragments arranged by size:
4502 816 411
PmeI GTTT'AAAC
Cuts at: 2728 2728
Size: 5729
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1147 1179 2371 2403 4868 4900 1147
Size: 32 1192 32 2465 32 1976
Fragments arranged by size:
2465 1976 1192 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1816 1816
Size: 5729
PshAI GACnn'nnGTC
Cuts at: 257 257
Size: 5729
PspOMI G'GGCC_C
Cuts at: 165 1001 2096 165
Size: 836 1095 3798
Fragments arranged by size:
3798 1095 836
PstI C_TGCA'G
Cuts at: 884 1174 1609 2776 3461 884
Size: 290 435 1167 685 3152
Fragments arranged by size:
3152 1167 685 435 290
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5729
PvuII CAG'CTG
Cuts at: 1411 2178 3154 3514 1411
Size: 767 976 360 3626
Fragments arranged by size:
3626 976 767 360
RsrII CG'GwC_CG
Cuts at: 3924 3924
Size: 5729
SacI G_AGCT'C
Cuts at: 1922 2743 1922
Size: 821 4908
SalI G'TCGA_C
Cuts at: 2766 2766
Size: 5729
SapI GCTCTTCn'nnn_
Cuts at: 3752 3962 3752
Size: 210 5519
SbfI CC_TGCA'GG
Cuts at: 2776 2776
Size: 5729
ScaI AGT'ACT
Cuts at: 5246 5246
Size: 5729
SfcI C'TryA_G
Cuts at: 33 537 880 1170 1605 2772 3457 4450 4641
Size: 504 343 290 435 1167 685 993 191
Cuts at: 4641 33
Size: 1121
Fragments arranged by size:
1167 1121 993 685 504 435 343 290
191
SfoI GGC'GCC
Cuts at: 1354 3161 3409 1354
Size: 1807 248 3674
Fragments arranged by size:
3674 1807 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5729
SmaI CCC'GGG
Cuts at: 934 2101 2751 934
Size: 1167 650 3912
Fragments arranged by size:
3912 1167 650
SmlI C'TyrA_G
Cuts at: 570 2884 3799 4291 4553 570
Size: 2314 915 492 262 1746
Fragments arranged by size:
2314 1746 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5729
SphI G_CATG'C
Cuts at: 2782 3282 2782
Size: 500 5229
StuI AGG'CCT
Cuts at: 775 1906 775
Size: 1131 4598
StyI C'CwwG_G
Cuts at: 107 386 492 683 689 1114 1268 1564 2643
Size: 279 106 191 6 425 154 296 1079
Cuts at: 2643 2649 2863 3217 107
Size: 6 214 354 2619
Fragments arranged by size:
2619 1079 425 354 296 279 214 191
154 106 6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 199 349 969 1432 1855 1927 2613 3312 3445
Size: 150 620 463 423 72 686 699 133
Cuts at: 3445 3904 4113 199
Size: 459 209 1815
Fragments arranged by size:
1815 699 686 620 463 459 423 209
150 133 72
TatI w'GTAC_w
Cuts at: 1784 2405 5244 1784
Size: 621 2839 2269
Fragments arranged by size:
2839 2269 621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 648 680 5246 5278 188
Size: 32 428 32 4566 32 639
Fragments arranged by size:
4566 639 428 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2712 3526 2712
Size: 814 4915
XbaI T'CTAG_A
Cuts at: 2760 2760
Size: 5729
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 897 3224 897
Size: 2327 3402
XmaI C'CCGG_G
Cuts at: 932 2099 2749 932
Size: 1167 650 3912
Fragments arranged by size:
3912 1167 650
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI