Restriction map for
pF1KE9407
(Circular) (Six-base) MAPSORT of: pF1KE9407.seq Check: 8736 from: 1 to: 4112
pF1KE9407 4112 bp
With 182 enzymes: SgfI *
November 11, 2015 11:13 ..
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1937 2369 3116 3384 418
Size: 1519 432 747 268 1146
Fragments arranged by size:
1519 1146 747 432 268
AflIII A'CryG_T
Cuts at: 122 124 410 1464 2568 3545 122
Size: 2 286 1054 1104 977 689
Fragments arranged by size:
1104 1054 977 689 286 2
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 463 463
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 1417 2984 1417
Size: 1567 2545
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 957 1116 3465 3721 957
Size: 159 2349 256 1348
Fragments arranged by size:
2349 1348 256 159
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 111 144 111
Size: 33 4079
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 1128 1542 1790 1825 1128
Size: 414 248 35 3415
Fragments arranged by size:
3415 414 248 35
BanII G_rGCy'C
Cuts at: 1126 2156 1126
Size: 1030 3082
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BbsI GAAGACnn'nnnn_
Cuts at: 733 1014 733
Size: 281 3831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4078
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 536 1632 536
Size: 1096 3016
BglI GCCn_nnn'nGGC
Cuts at: 366 366
Size: 4112
BglII A'GATC_T
Cuts at: 768 1627 3664 768
Size: 859 2037 1216
Fragments arranged by size:
2037 1216 859
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 1737 1830 2886 1737
Size: 93 1056 2963
Fragments arranged by size:
2963 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 642 967 1324 1405 1740 3884 642
Size: 325 357 81 335 2144 870
Fragments arranged by size:
2144 870 357 335 325 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2167 2659 2957 1288
Size: 879 492 298 2443
Fragments arranged by size:
2443 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 211 379 3816 211
Size: 168 3437 507
Fragments arranged by size:
3437 507 168
BsaAI yAC'GTr
Cuts at: 125 1465 125
Size: 1340 2772
BsaBI GATnn'nnATC
Cuts at: 6 752 1626 3663 6
Size: 746 874 2037 455
Fragments arranged by size:
2037 874 746 455
BsaHI Gr'CG_yC
Cuts at: 1543 1791 2493 3989 1543
Size: 248 702 1496 1666
Fragments arranged by size:
1666 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 559
Size: 4112
BseYI C'CCAG_C
Cuts at: 1537 2295 2872 3368 1537
Size: 758 577 496 2281
Fragments arranged by size:
2281 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 833
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1126 1904 2094 2886 1126
Size: 778 190 792 2352
Fragments arranged by size:
2352 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1126 1737 1830 1904 2094 2156 2886 1126
Size: 611 93 74 190 62 730 2352
Fragments arranged by size:
2352 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 351 3288 351
Size: 2937 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1376 1678 2059 1162
Size: 214 302 381 3215
Fragments arranged by size:
3215 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 293 521 2024 293
Size: 228 1503 2381
Fragments arranged by size:
2381 1503 228
BsrFI r'CCGG_y
Cuts at: 2110 2291 2500 2110
Size: 181 209 3722
Fragments arranged by size:
3722 209 181
BsrGI T'GTAC_A
Cuts at: 512 512
Size: 4112
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 1489 1489
Size: 4112
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 772 772
Size: 4112
BstYI r'GATC_y
Cuts at: 1 332 618 768 1137 1604 1627 1962 2208
Size: 331 286 150 369 467 23 335 246
Cuts at: 2208 3220 3306 3318 3664 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 369 346 335 331 286
246 150 86 23 12
BtgI C'CryG_G
Cuts at: 758 758
Size: 4112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 182 739 1355 182
Size: 557 616 2939
Fragments arranged by size:
2939 616 557
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 358 1697 1871 2262 2289 3634 358
Size: 1339 174 391 27 1345 836
Fragments arranged by size:
1345 1339 836 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1937 2369 3116 3384 418
Size: 1519 432 747 268 1146
Fragments arranged by size:
1519 1146 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1251 1251
Size: 4112
EcoRI G'AATT_C
Cuts at: 957 1116 957
Size: 159 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 1546 1794 2816 3795 1546
Size: 248 1022 979 1863
Fragments arranged by size:
1863 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 1954 1984 1986 2016 574
Size: 32 1348 30 2 30 2670
Fragments arranged by size:
2670 1348 32 30 30 2
HincII GTy'rAC
Cuts at: 408 639 1151 408
Size: 231 512 3369
Fragments arranged by size:
3369 512 231
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1068 2783 2967 1068
Size: 1715 184 2213
Fragments arranged by size:
2213 1715 184
MscI TGG'CCA
Cuts at: 360 1873 360
Size: 1513 2599
MslI CAynn'nnrTG
Cuts at: 196 445 2101 2228 196
Size: 249 1656 127 2080
Fragments arranged by size:
2080 1656 249 127
MspA1I CmG'CkG
Cuts at: 1223 1537 1897 2910 3155 1223
Size: 314 360 1013 245 2180
Fragments arranged by size:
2180 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 4112
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NcoI C'CATG_G
Cuts at: 758 758
Size: 4112
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 4112
NspI r_CATG'y
Cuts at: 384 414 1165 1665 2572 384
Size: 30 751 500 907 1924
Fragments arranged by size:
1924 907 751 500 30
PciI A'CATG_T
Cuts at: 410 2568 410
Size: 2158 1954
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PmlI CAC'GTG
Cuts at: 125 125
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PstI C_TGCA'G
Cuts at: 1159 1844 1159
Size: 685 3427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1537 1897 1537
Size: 360 3752
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SfcI C'TryA_G
Cuts at: 33 1155 1840 2833 3024 33
Size: 1122 685 993 191 1121
Fragments arranged by size:
1122 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 366 366
Size: 4112
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 1267 2182 2674 2936 1267
Size: 915 492 262 2443
Fragments arranged by size:
2443 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
SspI AAT'ATT
Cuts at: 923 923
Size: 4112
StyI C'CwwG_G
Cuts at: 758 1246 1600 758
Size: 488 354 3270
Fragments arranged by size:
3270 488 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1695 1828 2287 2496 1695
Size: 133 459 209 3311
Fragments arranged by size:
3311 459 209 133
TatI w'GTAC_w
Cuts at: 512 555 3627 512
Size: 43 3072 997
Fragments arranged by size:
3072 997 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 973 1909 973
Size: 936 3176
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 612 1607 612
Size: 995 3117
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI