Restriction map for
pF1KE9257
(Circular) (Six-base) MAPSORT of: pF1KE9257.seq Check: 3104 from: 1 to: 5690
pF1KE9257 5690 bp
With 182 enzymes: SgfI *
May 12, 2014 09:13 ..
AatII G_ACGT'C
Cuts at: 1901 1901
Size: 5690
AccI GT'mk_AC
Cuts at: 2728 2728
Size: 5690
Acc65I G'GTAC_C
Cuts at: 2549 2706 2549
Size: 157 5533
AclI AA'CG_TT
Cuts at: 5530 5530
Size: 5690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1528 1651 1822 2144 2603 3515 3947 4694 4962
Size: 123 171 322 459 912 432 747 268
Cuts at: 4962 1528
Size: 2256
Fragments arranged by size:
2256 912 747 459 432 322 268 171
123
AflIII A'CryG_T
Cuts at: 1637 3042 4146 5123 1637
Size: 1405 1104 977 2204
Fragments arranged by size:
2204 1405 1104 977
AgeI A'CCGG_T
Cuts at: 4078 4078
Size: 5690
AleI CACnn'nnGTG
Cuts at: 634 2311 2476 634
Size: 1677 165 3848
Fragments arranged by size:
3848 1677 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1270 1270
Size: 5690
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 326 358 1603 1635 326
Size: 32 1245 32 4381
Fragments arranged by size:
4381 1245 32 32
AlwNI CAG_nnn'CTG
Cuts at: 591 1785 2995 4562 591
Size: 1194 1210 1567 1719
Fragments arranged by size:
1719 1567 1210 1194
ApaLI G'TGCA_C
Cuts at: 1023 1491 4460 1023
Size: 468 2969 2253
Fragments arranged by size:
2969 2253 468
ApoI r'AATT_y
Cuts at: 1294 2694 5043 5299 1294
Size: 1400 2349 256 1685
Fragments arranged by size:
2349 1685 1400 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5690
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
AvaI C'yCGr_G
Cuts at: 114 306 1539 2206 2710 114
Size: 192 1233 667 504 3094
Fragments arranged by size:
3094 1233 667 504 192
BamHI G'GATC_C
Cuts at: 2715 2715
Size: 5690
BanI G'GyrC_C
Cuts at: 1221 1625 2237 2549 2706 3120 3368 3403 1221
Size: 404 612 312 157 414 248 35 3508
Fragments arranged by size:
3508 612 414 404 312 248 157 35
BanII G_rGCy'C
Cuts at: 1616 2054 2704 3734 1616
Size: 438 650 1030 3572
Fragments arranged by size:
3572 1030 650 438
BbeI G_GCGC'C
Cuts at: 1225 3124 3372 1225
Size: 1899 248 3543
Fragments arranged by size:
3543 1899 248
BciVI GTATCCnnnnn_n'
Cuts at: 3588 4349 5690 3588
Size: 761 1341 3588
Fragments arranged by size:
3588 1341 761
BclI T'GATC_A
Cuts at: 1199 3210 1199
Size: 2011 3679
BglI GCCn_nnn'nGGC
Cuts at: 1230 2542 1230
Size: 1312 4378
BglII A'GATC_T
Cuts at: 362 1535 3205 5242 362
Size: 1173 1670 2037 810
Fragments arranged by size:
2037 1670 1173 810
BlpI GC'TnA_GC
Cuts at: 2802 2802
Size: 5690
Bme1580I G_kGCm'C
Cuts at: 307 1027 1495 3315 3408 4464 307
Size: 720 468 1820 93 1056 1533
Fragments arranged by size:
1820 1533 1056 720 468 93
BmgBI CAC'GTC
Cuts at: 597 1329 2406 597
Size: 732 1077 3881
Fragments arranged by size:
3881 1077 732
BmrI ACTGGGnnnn_n'
Cuts at: 1029 1343 2902 2983 3318 5462 1029
Size: 314 1559 81 335 2144 1257
Fragments arranged by size:
2144 1559 1257 335 314 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4057 4057
Size: 5690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1150 472
Size: 678 5012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2866 3745 4237 4535 2866
Size: 879 492 298 4021
Fragments arranged by size:
4021 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2612 5394 2612
Size: 2782 2908
BsaAI yAC'GTr
Cuts at: 1640 2478 3043 1640
Size: 838 565 4287
Fragments arranged by size:
4287 838 565
BsaBI GATnn'nnATC
Cuts at: 6 3204 5241 6
Size: 3198 2037 455
Fragments arranged by size:
3198 2037 455
BsaHI Gr'CG_yC
Cuts at: 1222 1406 1684 1898 1964 3121 3369 4071 5567
Size: 184 278 214 66 1157 248 702 1496
Cuts at: 5567 1222
Size: 1345
Fragments arranged by size:
1496 1345 1157 702 278 248 214 184
66
BsaWI w'CCGG_w
Cuts at: 2879 3400 4078 4352 4499 4910 2879
Size: 521 678 274 147 411 3659
Fragments arranged by size:
3659 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1603 1633 1706 1736 1883 1913 1603
Size: 30 73 30 147 30 5380
Fragments arranged by size:
5380 147 73 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 324 2044 2421 324
Size: 1720 377 3593
Fragments arranged by size:
3593 1720 377
BseYI C'CCAG_C
Cuts at: 562 1481 1725 1818 2505 3115 3873 4450 4946
Size: 919 244 93 687 610 758 577 496
Cuts at: 4946 562
Size: 1306
Fragments arranged by size:
1306 919 758 687 610 577 496 244
93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1768 1783 2020 2241 2248 2469 1768
Size: 15 237 221 7 221 4989
Fragments arranged by size:
4989 237 221 221 15 7
BsiEI CG_ry'CG
Cuts at: 104 1230 3232 3278 4486 104
Size: 1126 2002 46 1208 1308
Fragments arranged by size:
2002 1308 1208 1126 46
BsiHKAI G_wGCw'C
Cuts at: 1027 1495 2054 2704 3482 3672 4464 1027
Size: 468 559 650 778 190 792 2253
Fragments arranged by size:
2253 792 778 650 559 468 190
BsmBI CGTCTCn'nnnn_
Cuts at: 571 1538 571
Size: 967 4723
Bsp1286I G_dGCh'C
Cuts at: 307 1027 1495 1616 2054 2704 3315 3408 3482
Size: 720 468 121 438 650 611 93 74
Cuts at: 3482 3672 3734 4464 307
Size: 190 62 730 1533
Fragments arranged by size:
1533 730 720 650 611 468 438 190
121 93 74 62
BspEI T'CCGG_A
Cuts at: 4910 4910
Size: 5690
BspHI T'CATG_A
Cuts at: 602 4866 602
Size: 4264 1426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2130 2740 2954 3256 3637 2130
Size: 610 214 302 381 4183
Fragments arranged by size:
4183 610 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1007 2274 3982 4036 5520 5685 50
Size: 957 1267 1708 54 1484 165 55
Fragments arranged by size:
1708 1484 1267 957 165 55 54
BsrDI GCAATG_nn'
Cuts at: 960 1463 1937 3602 960
Size: 503 474 1665 3048
Fragments arranged by size:
3048 1665 503 474
BsrFI r'CCGG_y
Cuts at: 121 1860 2009 3688 3869 4078 121
Size: 1739 149 1679 181 209 1733
Fragments arranged by size:
1739 1733 1679 209 181 149
BssSI C'ACGA_G
Cuts at: 2152 3961 4319 2152
Size: 1809 358 3523
Fragments arranged by size:
3523 1809 358
BstAPI GCAn_nnn'nTGC
Cuts at: 142 1746 3067 142
Size: 1604 1321 2765
Fragments arranged by size:
2765 1604 1321
BstBI TT'CG_AA
Cuts at: 242 4051 242
Size: 3809 1881
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2599
Size: 5690
BstYI r'GATC_y
Cuts at: 1 362 1278 1535 2447 2715 3182 3205 3540
Size: 361 916 257 912 268 467 23 335
Cuts at: 3540 3786 4798 4884 4896 5242 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 916 912 467 449 361 346 335
268 257 246 86 23 12
BtgI C'CryG_G
Cuts at: 635 1946 2039 635
Size: 1311 93 4286
Fragments arranged by size:
4286 1311 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3640 3821 3984 3640
Size: 181 163 5346
Fragments arranged by size:
5346 181 163
BtsI GCAGTG_nn'
Cuts at: 1747 2933 1747
Size: 1186 4504
DraI TTT'AAA
Cuts at: 354 828 2689 354
Size: 474 1861 3355
Fragments arranged by size:
3355 1861 474
DraIII CAC_nnn'GTG
Cuts at: 1742 2532 2559 1742
Size: 790 27 4873
Fragments arranged by size:
4873 790 27
DrdI GACnn_nn'nnGTC
Cuts at: 3396 4254 3396
Size: 858 4832
EaeI y'GGCC_r
Cuts at: 1733 2033 2162 2633 3275 3449 3840 3867 5212
Size: 300 129 471 642 174 391 27 1345
Cuts at: 5212 1733
Size: 2211
Fragments arranged by size:
2211 1345 642 471 391 300 174 129
27
EagI C'GGCC_G
Cuts at: 3275 3275
Size: 5690
EarI CTCTTCn'nnn_
Cuts at: 928 1232 2227 2257 3713 3923 928
Size: 304 995 30 1456 210 2695
Fragments arranged by size:
2695 1456 995 304 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1615 3898 4208 4354 5500 1615
Size: 2283 310 146 1146 1805
Fragments arranged by size:
2283 1805 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2052 2702 2052
Size: 650 5040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1150 1528 1651 1822 2144 2603 3515 3947
Size: 678 378 123 171 322 459 912 432
Cuts at: 3947 4694 4962 472
Size: 747 268 1200
Fragments arranged by size:
1200 912 747 678 459 432 378 322
268 171 123
EcoO109I rG'GnC_Cy
Cuts at: 203 588 2829 203
Size: 385 2241 3064
Fragments arranged by size:
3064 2241 385
EcoRI G'AATT_C
Cuts at: 2694 2694
Size: 5690
EcoRV GAT'ATC
Cuts at: 288 288
Size: 5690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2408 3709 2408
Size: 1301 4389
FspI TGC'GCA
Cuts at: 2668 3471 2668
Size: 803 4887
HaeII r_GCGC'y
Cuts at: 1225 1488 2512 3124 3372 4394 5373 1225
Size: 263 1024 612 248 1022 979 1542
Fragments arranged by size:
1542 1024 1022 979 612 263 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1883 1915 2104 2136 3532 3562 3564 3594 1883
Size: 32 189 32 1396 30 2 30 3979
Fragments arranged by size:
3979 1396 189 32 32 30 30 2
HincII GTy'rAC
Cuts at: 318 2729 318
Size: 2411 3279
HpaI GTT'AAC
Cuts at: 318 318
Size: 5690
KasI G'GCGC_C
Cuts at: 1221 3120 3368 1221
Size: 1899 248 3543
Fragments arranged by size:
3543 1899 248
KpnI G_GTAC'C
Cuts at: 2553 2710 2553
Size: 157 5533
MluI A'CGCG_T
Cuts at: 5123 5123
Size: 5690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1077 1814 4361 4545 1077
Size: 737 2547 184 2222
Fragments arranged by size:
2547 2222 737 184
MscI TGG'CCA
Cuts at: 1735 2164 2635 3451 1735
Size: 429 471 816 3974
Fragments arranged by size:
3974 816 471 429
MslI CAynn'nnrTG
Cuts at: 415 634 2224 2311 2476 2576 3679 3806 415
Size: 219 1590 87 165 100 1103 127 2299
Fragments arranged by size:
2299 1590 1103 219 165 127 100 87
MspA1I CmG'CkG
Cuts at: 993 1656 2463 2801 3115 3475 4488 4733 993
Size: 663 807 338 314 360 1013 245 1950
Fragments arranged by size:
1950 1013 807 663 360 338 314 245
NaeI GCC'GGC
Cuts at: 123 3871 123
Size: 3748 1942
NarI GG'CG_CC
Cuts at: 1222 3121 3369 1222
Size: 1899 248 3543
Fragments arranged by size:
3543 1899 248
NcoI C'CATG_G
Cuts at: 2039 2039
Size: 5690
NgoMIV G'CCGG_C
Cuts at: 121 3869 121
Size: 3748 1942
NspI r_CATG'y
Cuts at: 1419 1557 2743 3243 4150 1419
Size: 138 1186 500 907 2959
Fragments arranged by size:
2959 1186 907 500 138
PasI CC'CwG_GG
Cuts at: 1848 1848
Size: 5690
PciI A'CATG_T
Cuts at: 4146 4146
Size: 5690
PflMI CCAn_nnn'nTGG
Cuts at: 506 591 2559 2631 506
Size: 85 1968 72 3565
Fragments arranged by size:
3565 1968 85 72
PfoI T'CCnGG_A
Cuts at: 208 208
Size: 5690
PmeI GTTT'AAAC
Cuts at: 2689 2689
Size: 5690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1603 1635 4829 4861 1603
Size: 32 3194 32 2432
Fragments arranged by size:
3194 2432 32 32
PpuMI rG'GwC_Cy
Cuts at: 203 203
Size: 5690
PshAI GACnn'nnGTC
Cuts at: 1396 1396
Size: 5690
PstI C_TGCA'G
Cuts at: 998 1349 1655 2447 2737 3422 998
Size: 351 306 792 290 685 3266
Fragments arranged by size:
3266 792 685 351 306 290
PvuI CG_AT'CG
Cuts at: 104 1230 104
Size: 1126 4564
PvuII CAG'CTG
Cuts at: 1656 2463 3115 3475 1656
Size: 807 652 360 3871
Fragments arranged by size:
3871 807 652 360
RsrII CG'GwC_CG
Cuts at: 3885 3885
Size: 5690
SacI G_AGCT'C
Cuts at: 2054 2704 2054
Size: 650 5040
SalI G'TCGA_C
Cuts at: 2727 2727
Size: 5690
SapI GCTCTTCn'nnn_
Cuts at: 2257 3713 3923 2257
Size: 1456 210 4024
Fragments arranged by size:
4024 1456 210
SbfI CC_TGCA'GG
Cuts at: 2737 2737
Size: 5690
ScaI AGT'ACT
Cuts at: 5207 5207
Size: 5690
SexAI A'CCwGG_T
Cuts at: 2528 2555 2528
Size: 27 5663
SfcI C'TryA_G
Cuts at: 33 994 1345 1651 2443 2733 3418 4411 4602
Size: 961 351 306 792 290 685 993 191
Cuts at: 4602 33
Size: 1121
Fragments arranged by size:
1121 993 961 792 685 351 306 290
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2542 2542
Size: 5690
SfoI GGC'GCC
Cuts at: 1223 3122 3370 1223
Size: 1899 248 3543
Fragments arranged by size:
3543 1899 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
SgrAI Cr'CCGG_yG
Cuts at: 1860 1860
Size: 5690
SmaI CCC'GGG
Cuts at: 2208 2712 2208
Size: 504 5186
SmlI C'TyrA_G
Cuts at: 1539 2845 3760 4252 4514 1539
Size: 1306 915 492 262 2715
Fragments arranged by size:
2715 1306 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5690
SphI G_CATG'C
Cuts at: 1557 2743 3243 1557
Size: 1186 500 4004
Fragments arranged by size:
4004 1186 500
SspI AAT'ATT
Cuts at: 756 756
Size: 5690
StuI AGG'CCT
Cuts at: 2176 2391 2176
Size: 215 5475
StyI C'CwwG_G
Cuts at: 162 2039 2636 2824 3178 162
Size: 1877 597 188 354 2674
Fragments arranged by size:
2674 1877 597 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 498 2344 3273 3406 3865 4074 498
Size: 1846 929 133 459 209 2114
Fragments arranged by size:
2114 1846 929 459 209 133
TatI w'GTAC_w
Cuts at: 110 2675 5205 110
Size: 2565 2530 595
Fragments arranged by size:
2565 2530 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 601 633 930 962 1107 1139 1706 1738 5207
Size: 32 297 32 145 32 567 32 3469
Cuts at: 5207 5239 601
Size: 32 1052
Fragments arranged by size:
3469 1052 567 297 145 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 598 1785 2116 3487 598
Size: 1187 331 1371 2801
Fragments arranged by size:
2801 1371 1187 331
XbaI T'CTAG_A
Cuts at: 2721 2721
Size: 5690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1944 2553 3185 1944
Size: 609 632 4449
Fragments arranged by size:
4449 632 609
XhoI C'TCGA_G
Cuts at: 1539 1539
Size: 5690
XmaI C'CCGG_G
Cuts at: 2206 2710 2206
Size: 504 5186
XmnI GAAnn'nnTTC
Cuts at: 1513 1513
Size: 5690
ZraI GAC'GTC
Cuts at: 1899 1899
Size: 5690
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SnaBI SrfI SwaI