Restriction map for
pF1KE6821
(Circular) (Six-base) MAPSORT of: pF1KE6821.seq Check: 5080 from: 1 to: 4250
pF1KE6821 4250 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1112 1112
Size: 4250
AccI GT'mk_AC
Cuts at: 1288 1288
Size: 4250
Acc65I G'GTAC_C
Cuts at: 242 335 1266 242
Size: 93 931 3226
Fragments arranged by size:
3226 931 93
AclI AA'CG_TT
Cuts at: 4090 4090
Size: 4250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 2507 3254 3522 2075
Size: 432 747 268 2803
Fragments arranged by size:
2803 747 432 268
AflIII A'CryG_T
Cuts at: 1602 2706 3683 1602
Size: 1104 977 2169
Fragments arranged by size:
2169 1104 977
AgeI A'CCGG_T
Cuts at: 793 2638 793
Size: 1845 2405
AlwNI CAG_nnn'CTG
Cuts at: 350 495 699 1555 3122 350
Size: 145 204 856 1567 1478
Fragments arranged by size:
1567 1478 856 204 145
ApaI G_GGCC'C
Cuts at: 162 399 162
Size: 237 4013
ApaLI G'TGCA_C
Cuts at: 3020 3020
Size: 4250
ApoI r'AATT_y
Cuts at: 561 1254 3603 3859 561
Size: 693 2349 256 952
Fragments arranged by size:
2349 952 693 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4250
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
AvaI C'yCGr_G
Cuts at: 1270 1270
Size: 4250
BamHI G'GATC_C
Cuts at: 1275 1275
Size: 4250
BanI G'GyrC_C
Cuts at: 242 335 729 871 1100 1158 1266 1680 1928
Size: 93 394 142 229 58 108 414 248
Cuts at: 1928 1963 242
Size: 35 2529
Fragments arranged by size:
2529 414 394 248 229 142 108 93
58 35
BanII G_rGCy'C
Cuts at: 162 399 632 1196 1264 2294 162
Size: 237 233 564 68 1030 2118
Fragments arranged by size:
2118 1030 564 237 233 68
BbeI G_GCGC'C
Cuts at: 1684 1932 1684
Size: 248 4002
BbsI GAAGACnn'nnnn_
Cuts at: 1153 1153
Size: 4250
BbvCI CC'TCA_GC
Cuts at: 308 1152 308
Size: 844 3406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4216
BciVI GTATCCnnnnn_n'
Cuts at: 2148 2909 4250 2148
Size: 761 1341 2148
Fragments arranged by size:
2148 1341 761
BclI T'GATC_A
Cuts at: 1770 1770
Size: 4250
BglI GCCn_nnn'nGGC
Cuts at: 166 518 166
Size: 352 3898
BglII A'GATC_T
Cuts at: 536 1765 3802 536
Size: 1229 2037 984
Fragments arranged by size:
2037 1229 984
BlpI GC'TnA_GC
Cuts at: 1362 1362
Size: 4250
Bme1580I G_kGCm'C
Cuts at: 162 399 874 1163 1875 1968 3024 162
Size: 237 475 289 712 93 1056 1388
Fragments arranged by size:
1388 1056 712 475 289 237 93
BmrI ACTGGGnnnn_n'
Cuts at: 1157 1462 1543 1878 4022 1157
Size: 305 81 335 2144 1385
Fragments arranged by size:
2144 1385 335 305 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 4250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 315 372 616 871 904 102
Size: 213 57 244 255 33 3448
Fragments arranged by size:
3448 255 244 213 57 33
Bpu10I CC'TnA_GC
Cuts at: 308 1152 1190 308
Size: 844 38 3368
Fragments arranged by size:
3368 844 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 982 1012 1426 2305 2797 3095 499
Size: 483 30 414 879 492 298 1654
Fragments arranged by size:
1654 879 492 483 414 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 3954 3954
Size: 4250
BsaAI yAC'GTr
Cuts at: 1603 1603
Size: 4250
BsaBI GATnn'nnATC
Cuts at: 6 1764 3801 6
Size: 1758 2037 455
Fragments arranged by size:
2037 1758 455
BsaHI Gr'CG_yC
Cuts at: 1681 1929 2631 4127 1681
Size: 248 702 1496 1804
Fragments arranged by size:
1804 1496 702 248
BsaWI w'CCGG_w
Cuts at: 239 793 1439 1960 2638 2912 3059 3470 239
Size: 554 646 521 678 274 147 411 1019
Fragments arranged by size:
1019 678 646 554 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 247 277 313 343 247
Size: 30 36 30 4154
Fragments arranged by size:
4154 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 700 1186 325
Size: 375 486 3389
Fragments arranged by size:
3389 486 375
BseYI C'CCAG_C
Cuts at: 273 528 785 1675 2433 3010 3506 273
Size: 255 257 890 758 577 496 1017
Fragments arranged by size:
1017 890 758 577 496 257 255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 704
Size: 4250
BsiEI CG_ry'CG
Cuts at: 104 446 1792 1838 3046 104
Size: 342 1346 46 1208 1308
Fragments arranged by size:
1346 1308 1208 342 46
BsiHKAI G_wGCw'C
Cuts at: 233 254 632 1196 1264 2042 2232 3024 233
Size: 21 378 564 68 778 190 792 1459
Fragments arranged by size:
1459 792 778 564 378 190 68 21
Bsp1286I G_dGCh'C
Cuts at: 162 233 254 399 632 874 1163 1196 1264
Size: 71 21 145 233 242 289 33 68
Cuts at: 1264 1875 1968 2042 2232 2294 3024 162
Size: 611 93 74 190 62 730 1388
Fragments arranged by size:
1388 730 611 289 242 233 190 145
93 74 71 68 62 33 21
BspEI T'CCGG_A
Cuts at: 3470 3470
Size: 4250
BspHI T'CATG_A
Cuts at: 3426 3426
Size: 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 680 1065 1112 1300 1514 1816 2197 680
Size: 385 47 188 214 302 381 2733
Fragments arranged by size:
2733 385 381 302 214 188 47
BsrBI CCG'CTC
Cuts at: 50 2542 2596 4080 4245 50
Size: 2492 54 1484 165 55
Fragments arranged by size:
2492 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1040 2162 1040
Size: 1122 3128
BsrFI r'CCGG_y
Cuts at: 793 2248 2429 2638 793
Size: 1455 181 209 2405
Fragments arranged by size:
2405 1455 209 181
BssSI C'ACGA_G
Cuts at: 2521 2879 2521
Size: 358 3892
BstAPI GCAn_nnn'nTGC
Cuts at: 1627 1627
Size: 4250
BstBI TT'CG_AA
Cuts at: 645 2611 645
Size: 1966 2284
BstEII G'GTnAC_C
Cuts at: 198 796 198
Size: 598 3652
BstYI r'GATC_y
Cuts at: 1 536 1275 1742 1765 2100 2346 3358 3444
Size: 535 739 467 23 335 246 1012 86
Cuts at: 3444 3456 3802 1
Size: 12 346 449
Fragments arranged by size:
1012 739 535 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 390 576 390
Size: 186 4064
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2200 2381 2544 2200
Size: 181 163 3906
Fragments arranged by size:
3906 181 163
BtsI GCAGTG_nn'
Cuts at: 551 692 1493 551
Size: 141 801 3308
Fragments arranged by size:
3308 801 141
DraI TTT'AAA
Cuts at: 1249 1249
Size: 4250
DrdI GACnn_nn'nnGTC
Cuts at: 1956 2814 1956
Size: 858 3392
EaeI y'GGCC_r
Cuts at: 1835 2009 2400 2427 3772 1835
Size: 174 391 27 1345 2313
Fragments arranged by size:
2313 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1835 1835
Size: 4250
EarI CTCTTCn'nnn_
Cuts at: 982 1118 2273 2483 982
Size: 136 1155 210 2749
Fragments arranged by size:
2749 1155 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 376 2458 2768 2914 4060 376
Size: 2082 310 146 1146 566
Fragments arranged by size:
2082 1146 566 310 146
EcoICRI GAG'CTC
Cuts at: 630 1194 1262 630
Size: 564 68 3618
Fragments arranged by size:
3618 564 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 315 372 616 871 904 2075 2507 3254
Size: 213 57 244 255 33 1171 432 747
Cuts at: 3254 3522 102
Size: 268 830
Fragments arranged by size:
1171 830 747 432 268 255 244 213
57 33
EcoNI CCTnn'n_nnAGG
Cuts at: 307 487 1108 307
Size: 180 621 3449
Fragments arranged by size:
3449 621 180
EcoO109I rG'GnC_Cy
Cuts at: 158 395 396 519 1389 158
Size: 237 1 123 870 3019
Fragments arranged by size:
3019 870 237 123 1
EcoRI G'AATT_C
Cuts at: 1254 1254
Size: 4250
EcoRV GAT'ATC
Cuts at: 607 607
Size: 4250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 189 2269 189
Size: 2080 2170
FspI TGC'GCA
Cuts at: 2031 2031
Size: 4250
HaeII r_GCGC'y
Cuts at: 1684 1932 2954 3933 1684
Size: 248 1022 979 2001
Fragments arranged by size:
2001 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2092 2122 2124 2154 2092
Size: 30 2 30 4188
Fragments arranged by size:
4188 30 30 2
HincII GTy'rAC
Cuts at: 1289 1289
Size: 4250
KasI G'GCGC_C
Cuts at: 1680 1928 1680
Size: 248 4002
KpnI G_GTAC'C
Cuts at: 246 339 1270 246
Size: 93 931 3226
Fragments arranged by size:
3226 931 93
MluI A'CGCG_T
Cuts at: 3683 3683
Size: 4250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2921 3105 2921
Size: 184 4066
MscI TGG'CCA
Cuts at: 2011 2011
Size: 4250
MslI CAynn'nnrTG
Cuts at: 463 2239 2366 463
Size: 1776 127 2347
Fragments arranged by size:
2347 1776 127
MspA1I CmG'CkG
Cuts at: 273 532 696 864 1156 1361 1675 2035 3048
Size: 259 164 168 292 205 314 360 1013
Cuts at: 3048 3293 273
Size: 245 1230
Fragments arranged by size:
1230 1013 360 314 292 259 245 205
168 164
NaeI GCC'GGC
Cuts at: 2431 2431
Size: 4250
NarI GG'CG_CC
Cuts at: 1681 1929 1681
Size: 248 4002
NcoI C'CATG_G
Cuts at: 576 576
Size: 4250
NgoMIV G'CCGG_C
Cuts at: 2429 2429
Size: 4250
NspI r_CATG'y
Cuts at: 1303 1803 2710 1303
Size: 500 907 2843
Fragments arranged by size:
2843 907 500
PasI CC'CwG_GG
Cuts at: 146 146
Size: 4250
PciI A'CATG_T
Cuts at: 2706 2706
Size: 4250
PflMI CCAn_nnn'nTGG
Cuts at: 582 582
Size: 4250
PfoI T'CCnGG_A
Cuts at: 770 881 770
Size: 111 4139
PmeI GTTT'AAAC
Cuts at: 1249 1249
Size: 4250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 4218
PspOMI G'GGCC_C
Cuts at: 158 395 158
Size: 237 4013
PstI C_TGCA'G
Cuts at: 272 301 546 1062 1297 1982 272
Size: 29 245 516 235 685 2540
Fragments arranged by size:
2540 685 516 245 235 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4250
PvuII CAG'CTG
Cuts at: 273 532 1675 2035 273
Size: 259 1143 360 2488
Fragments arranged by size:
2488 1143 360 259
RsrII CG'GwC_CG
Cuts at: 2445 2445
Size: 4250
SacI G_AGCT'C
Cuts at: 632 1196 1264 632
Size: 564 68 3618
Fragments arranged by size:
3618 564 68
SalI G'TCGA_C
Cuts at: 1287 1287
Size: 4250
SapI GCTCTTCn'nnn_
Cuts at: 2273 2483 2273
Size: 210 4040
SbfI CC_TGCA'GG
Cuts at: 1297 1297
Size: 4250
ScaI AGT'ACT
Cuts at: 3767 3767
Size: 4250
SexAI A'CCwGG_T
Cuts at: 245 245
Size: 4250
SfcI C'TryA_G
Cuts at: 33 268 297 542 1058 1293 1978 2971 3162
Size: 235 29 245 516 235 685 993 191
Cuts at: 3162 33
Size: 1121
Fragments arranged by size:
1121 993 685 516 245 235 235 191
29
SfiI GGCCn_nnn'nGGCC
Cuts at: 518 518
Size: 4250
SfoI GGC'GCC
Cuts at: 1682 1930 1682
Size: 248 4002
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
SmaI CCC'GGG
Cuts at: 1272 1272
Size: 4250
SmlI C'TyrA_G
Cuts at: 514 961 991 1405 2320 2812 3074 514
Size: 447 30 414 915 492 262 1690
Fragments arranged by size:
1690 915 492 447 414 262 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4250
SphI G_CATG'C
Cuts at: 1303 1803 1303
Size: 500 3750
StyI C'CwwG_G
Cuts at: 173 576 1384 1738 173
Size: 403 808 354 2685
Fragments arranged by size:
2685 808 403 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1833 1966 2425 2634 1833
Size: 133 459 209 3449
Fragments arranged by size:
3449 459 209 133
TatI w'GTAC_w
Cuts at: 3765 3765
Size: 4250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 247 279 313 345 485 517 3767 3799 247
Size: 32 34 32 140 32 3250 32 698
Fragments arranged by size:
3250 698 140 34 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2047 2047
Size: 4250
XbaI T'CTAG_A
Cuts at: 1281 1281
Size: 4250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1745 1745
Size: 4250
XmaI C'CCGG_G
Cuts at: 1270 1270
Size: 4250
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI