Restriction map for
pF1KE6772
(Circular) (Six-base) MAPSORT of: pF1KE6772.seq Check: 4211 from: 1 to: 4952
pF1KE6772 4952 bp
With 182 enzymes: SgfI *
July 24, 2018 16:24 ..
AccI GT'mk_AC
Cuts at: 1990 1990
Size: 4952
Acc65I G'GTAC_C
Cuts at: 1968 1968
Size: 4952
AclI AA'CG_TT
Cuts at: 4792 4792
Size: 4952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 617 2777 3209 3956 4224 443
Size: 174 2160 432 747 268 1171
Fragments arranged by size:
2160 1171 747 432 268 174
AflIII A'CryG_T
Cuts at: 2304 3408 4385 2304
Size: 1104 977 2871
Fragments arranged by size:
2871 1104 977
AgeI A'CCGG_T
Cuts at: 3340 3340
Size: 4952
AleI CACnn'nnGTG
Cuts at: 1093 1093
Size: 4952
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 458 490 458
Size: 32 4920
AlwNI CAG_nnn'CTG
Cuts at: 153 756 1157 1684 2257 3824 153
Size: 603 401 527 573 1567 1281
Fragments arranged by size:
1567 1281 603 573 527 401
ApaI G_GGCC'C
Cuts at: 1570 1570
Size: 4952
ApaLI G'TGCA_C
Cuts at: 3722 3722
Size: 4952
ApoI r'AATT_y
Cuts at: 401 1956 4305 4561 401
Size: 1555 2349 256 792
Fragments arranged by size:
2349 1555 792 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4952
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
AvaI C'yCGr_G
Cuts at: 189 563 1076 1111 1972 189
Size: 374 513 35 861 3169
Fragments arranged by size:
3169 861 513 374 35
BamHI G'GATC_C
Cuts at: 567 1977 567
Size: 1410 3542
BanI G'GyrC_C
Cuts at: 156 349 489 1968 2382 2630 2665 156
Size: 193 140 1479 414 248 35 2443
Fragments arranged by size:
2443 1479 414 248 193 140 35
BanII G_rGCy'C
Cuts at: 564 1313 1570 1966 2996 564
Size: 749 257 396 1030 2520
Fragments arranged by size:
2520 1030 749 396 257
BbeI G_GCGC'C
Cuts at: 493 2386 2634 493
Size: 1893 248 2811
Fragments arranged by size:
2811 1893 248
BbsI GAAGACnn'nnnn_
Cuts at: 1949 1949
Size: 4952
BbvCI CC'TCA_GC
Cuts at: 1150 1150
Size: 4952
BciVI GTATCCnnnnn_n'
Cuts at: 1699 2850 3611 4952 1699
Size: 1151 761 1341 1699
Fragments arranged by size:
1699 1341 1151 761
BclI T'GATC_A
Cuts at: 1473 2472 1473
Size: 999 3953
BglI GCCn_nnn'nGGC
Cuts at: 1536 1536
Size: 4952
BglII A'GATC_T
Cuts at: 1905 2467 4504 1905
Size: 562 2037 2353
Fragments arranged by size:
2353 2037 562
BlpI GC'TnA_GC
Cuts at: 1526 2064 1526
Size: 538 4414
Bme1580I G_kGCm'C
Cuts at: 161 1090 1570 2577 2670 3726 161
Size: 929 480 1007 93 1056 1387
Fragments arranged by size:
1387 1056 1007 929 480 93
BmrI ACTGGGnnnn_n'
Cuts at: 803 2164 2245 2580 4724 803
Size: 1361 81 335 2144 1031
Fragments arranged by size:
2144 1361 1031 335 81
BmtI G_CTAG'C
Cuts at: 275 275
Size: 4952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3319 3319
Size: 4952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 947 1327 1523 1538 1575 235
Size: 712 380 196 15 37 3612
Fragments arranged by size:
3612 712 380 196 37 15
Bpu10I CC'TnA_GC
Cuts at: 605 749 1150 605
Size: 144 401 4407
Fragments arranged by size:
4407 401 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 2128 3007 3499 3797 464
Size: 1664 879 492 298 1619
Fragments arranged by size:
1664 1619 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4656 4656
Size: 4952
BsaAI yAC'GTr
Cuts at: 2305 2305
Size: 4952
BsaBI GATnn'nnATC
Cuts at: 6 2466 4503 6
Size: 2460 2037 455
Fragments arranged by size:
2460 2037 455
BsaHI Gr'CG_yC
Cuts at: 490 2383 2631 3333 4829 490
Size: 1893 248 702 1496 613
Fragments arranged by size:
1893 1496 702 613 248
BsaWI w'CCGG_w
Cuts at: 113 2141 2662 3340 3614 3761 4172 113
Size: 2028 521 678 274 147 411 893
Fragments arranged by size:
2028 893 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 458 488 920 950 1722 1752 458
Size: 30 432 30 772 30 3658
Fragments arranged by size:
3658 772 432 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 617 617
Size: 4952
BseYI C'CCAG_C
Cuts at: 1741 2377 3135 3712 4208 1741
Size: 636 758 577 496 2485
Fragments arranged by size:
2485 758 636 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 1628
Size: 4952
BsiEI CG_ry'CG
Cuts at: 104 2494 2540 3748 104
Size: 2390 46 1208 1308
Fragments arranged by size:
2390 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1062 1469 1966 2744 2934 3726 1062
Size: 407 497 778 190 792 2288
Fragments arranged by size:
2288 792 778 497 407 190
BsmI GAATG_Cn'
Cuts at: 1865 1865
Size: 4952
Bsp1286I G_dGCh'C
Cuts at: 161 564 1062 1090 1313 1469 1570 1966 2577
Size: 403 498 28 223 156 101 396 611
Cuts at: 2577 2670 2744 2934 2996 3726 161
Size: 93 74 190 62 730 1387
Fragments arranged by size:
1387 730 611 498 403 396 223 190
156 101 93 74 62 28
BspEI T'CCGG_A
Cuts at: 4172 4172
Size: 4952
BspHI T'CATG_A
Cuts at: 1938 4128 1938
Size: 2190 2762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 764 2002 2216 2518 2899 764
Size: 1238 214 302 381 2817
Fragments arranged by size:
2817 1238 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3244 3298 4782 4947 50
Size: 3194 54 1484 165 55
Fragments arranged by size:
3194 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2864 2864
Size: 4952
BsrFI r'CCGG_y
Cuts at: 203 2950 3131 3340 203
Size: 2747 181 209 1815
Fragments arranged by size:
2747 1815 209 181
BssSI C'ACGA_G
Cuts at: 3223 3581 3223
Size: 358 4594
BstAPI GCAn_nnn'nTGC
Cuts at: 1420 2329 1420
Size: 909 4043
BstBI TT'CG_AA
Cuts at: 3313 3313
Size: 4952
BstYI r'GATC_y
Cuts at: 1 550 567 600 1133 1905 1977 2444 2467
Size: 549 17 33 533 772 72 467 23
Cuts at: 2467 2802 3048 4060 4146 4158 4504 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 772 549 533 467 449 346 335
246 86 72 33 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 1810 1810
Size: 4952
BtgI C'CryG_G
Cuts at: 1163 1163
Size: 4952
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 243 2902 3083 3246 243
Size: 2659 181 163 1949
Fragments arranged by size:
2659 1949 181 163
BtsI GCAGTG_nn'
Cuts at: 676 1239 2195 676
Size: 563 956 3433
Fragments arranged by size:
3433 956 563
ClaI AT'CG_AT
Cuts at: 299 299
Size: 4952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1317 1352 1317
Size: 35 4917
DraI TTT'AAA
Cuts at: 1951 1951
Size: 4952
DraIII CAC_nnn'GTG
Cuts at: 1290 1290
Size: 4952
DrdI GACnn_nn'nnGTC
Cuts at: 664 1822 2658 3516 664
Size: 1158 836 858 2100
Fragments arranged by size:
2100 1158 858 836
EaeI y'GGCC_r
Cuts at: 1325 2537 2711 3102 3129 4474 1325
Size: 1212 174 391 27 1345 1803
Fragments arranged by size:
1803 1345 1212 391 174 27
EagI C'GGCC_G
Cuts at: 2537 2537
Size: 4952
EarI CTCTTCn'nnn_
Cuts at: 934 1111 2975 3185 934
Size: 177 1864 210 2701
Fragments arranged by size:
2701 1864 210 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3160 3470 3616 4762 3160
Size: 310 146 1146 3350
Fragments arranged by size:
3350 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1964 1964
Size: 4952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 443 617 947 1327 1523 1538 1575 2777
Size: 208 174 330 380 196 15 37 1202
Cuts at: 2777 3209 3956 4224 235
Size: 432 747 268 963
Fragments arranged by size:
1202 963 747 432 380 330 268 208
196 174 37 15
EcoO109I rG'GnC_Cy
Cuts at: 753 1567 2091 753
Size: 814 524 3614
Fragments arranged by size:
3614 814 524
EcoRI G'AATT_C
Cuts at: 1956 1956
Size: 4952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4952
FspI TGC'GCA
Cuts at: 2733 2733
Size: 4952
HaeII r_GCGC'y
Cuts at: 493 2386 2634 3656 4635 493
Size: 1893 248 1022 979 810
Fragments arranged by size:
1893 1022 979 810 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1696 1728 2794 2824 2826 2856 1696
Size: 32 1066 30 2 30 3792
Fragments arranged by size:
3792 1066 32 30 30 2
HincII GTy'rAC
Cuts at: 1991 1991
Size: 4952
HindIII A'AGCT_T
Cuts at: 981 981
Size: 4952
KasI G'GCGC_C
Cuts at: 489 2382 2630 489
Size: 1893 248 2811
Fragments arranged by size:
2811 1893 248
KpnI G_GTAC'C
Cuts at: 1972 1972
Size: 4952
MluI A'CGCG_T
Cuts at: 4385 4385
Size: 4952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1203 3623 3807 1203
Size: 2420 184 2348
Fragments arranged by size:
2420 2348 184
MscI TGG'CCA
Cuts at: 1327 2713 1327
Size: 1386 3566
MslI CAynn'nnrTG
Cuts at: 1093 1408 2941 3068 1093
Size: 315 1533 127 2977
Fragments arranged by size:
2977 1533 315 127
MspA1I CmG'CkG
Cuts at: 532 813 1297 2063 2377 2737 3750 3995 532
Size: 281 484 766 314 360 1013 245 1489
Fragments arranged by size:
1489 1013 766 484 360 314 281 245
NaeI GCC'GGC
Cuts at: 3133 3133
Size: 4952
NarI GG'CG_CC
Cuts at: 490 2383 2631 490
Size: 1893 248 2811
Fragments arranged by size:
2811 1893 248
NcoI C'CATG_G
Cuts at: 1163 1163
Size: 4952
NgoMIV G'CCGG_C
Cuts at: 3131 3131
Size: 4952
NheI G'CTAG_C
Cuts at: 271 271
Size: 4952
NspI r_CATG'y
Cuts at: 238 621 1430 2005 2505 3412 238
Size: 383 809 575 500 907 1778
Fragments arranged by size:
1778 907 809 575 500 383
PciI A'CATG_T
Cuts at: 3408 3408
Size: 4952
PflMI CCAn_nnn'nTGG
Cuts at: 1290 1290
Size: 4952
PmeI GTTT'AAAC
Cuts at: 1951 1951
Size: 4952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 458 490 4091 4123 458
Size: 32 3601 32 1287
Fragments arranged by size:
3601 1287 32 32
PpuMI rG'GwC_Cy
Cuts at: 753 753
Size: 4952
PspOMI G'GGCC_C
Cuts at: 1566 1566
Size: 4952
PspXI vC'TCGA_Gb
Cuts at: 1076 1076
Size: 4952
PstI C_TGCA'G
Cuts at: 546 1999 2684 546
Size: 1453 685 2814
Fragments arranged by size:
2814 1453 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4952
PvuII CAG'CTG
Cuts at: 532 813 1297 2377 2737 532
Size: 281 484 1080 360 2747
Fragments arranged by size:
2747 1080 484 360 281
RsrII CG'GwC_CG
Cuts at: 170 3147 170
Size: 2977 1975
SacI G_AGCT'C
Cuts at: 1966 1966
Size: 4952
SalI G'TCGA_C
Cuts at: 1989 1989
Size: 4952
SapI GCTCTTCn'nnn_
Cuts at: 2975 3185 2975
Size: 210 4742
SbfI CC_TGCA'GG
Cuts at: 1999 1999
Size: 4952
ScaI AGT'ACT
Cuts at: 4469 4469
Size: 4952
SfcI C'TryA_G
Cuts at: 33 542 1995 2680 3673 3864 33
Size: 509 1453 685 993 191 1121
Fragments arranged by size:
1453 1121 993 685 509 191
SfoI GGC'GCC
Cuts at: 491 2384 2632 491
Size: 1893 248 2811
Fragments arranged by size:
2811 1893 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
SmaI CCC'GGG
Cuts at: 1974 1974
Size: 4952
SmlI C'TyrA_G
Cuts at: 479 1076 2107 3022 3514 3776 479
Size: 597 1031 915 492 262 1655
Fragments arranged by size:
1655 1031 915 597 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4952
SphI G_CATG'C
Cuts at: 1430 2005 2505 1430
Size: 575 500 3877
Fragments arranged by size:
3877 575 500
StuI AGG'CCT
Cuts at: 125 1720 125
Size: 1595 3357
StyI C'CwwG_G
Cuts at: 161 1038 1082 1163 2086 2440 161
Size: 877 44 81 923 354 2673
Fragments arranged by size:
2673 923 877 354 81 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1398 2535 2668 3127 3336 1398
Size: 1137 133 459 209 3014
Fragments arranged by size:
3014 1137 459 209 133
TatI w'GTAC_w
Cuts at: 807 4467 807
Size: 3660 1292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 920 952 4469 4501 920
Size: 32 3517 32 1371
Fragments arranged by size:
3517 1371 32 32
Tth111I GACn'n_nGTC
Cuts at: 1710 2749 1710
Size: 1039 3913
XbaI T'CTAG_A
Cuts at: 243 1983 243
Size: 1740 3212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1320 2447 1320
Size: 1127 3825
XhoI C'TCGA_G
Cuts at: 1076 1076
Size: 4952
XmaI C'CCGG_G
Cuts at: 1972 1972
Size: 4952
XmnI GAAnn'nnTTC
Cuts at: 1097 1097
Size: 4952
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BcgI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI