Restriction map for
pF1KE6673
(Circular) (Six-base) MAPSORT of: pF1KE6673.seq Check: 4566 from: 1 to: 4040
pF1KE6673 4040 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 453 459 453
Size: 6 4034
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 1865 2297 3044 3312 289
Size: 1576 432 747 268 1017
Fragments arranged by size:
1576 1017 747 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaI G_GGCC'C
Cuts at: 136 136
Size: 4040
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 564 1044 3393 3649 564
Size: 480 2349 256 955
Fragments arranged by size:
2349 955 480 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 778 960 984 1060 778
Size: 182 24 76 3758
Fragments arranged by size:
3758 182 76 24
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 353 619 868 964 1056 1470 1718 1753 353
Size: 266 249 96 92 414 248 35 2640
Fragments arranged by size:
2640 414 266 249 248 96 92 35
BanII G_rGCy'C
Cuts at: 115 136 209 319 957 1054 2084 115
Size: 21 73 110 638 97 1030 2071
Fragments arranged by size:
2071 1030 638 110 97 73 21
BbeI G_GCGC'C
Cuts at: 872 1474 1722 872
Size: 602 248 3190
Fragments arranged by size:
3190 602 248
BbsI GAAGACnn'nnnn_
Cuts at: 882 882
Size: 4040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 201 235 201
Size: 34 4006
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglI GCCn_nnn'nGGC
Cuts at: 644 764 935 952 644
Size: 120 171 17 3732
Fragments arranged by size:
3732 171 120 17
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 136 622 742 967 1665 1758 2814 136
Size: 486 120 225 698 93 1056 1362
Fragments arranged by size:
1362 1056 698 486 225 120 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 715
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 536 869 1471 1719 2421 3917 536
Size: 333 602 248 702 1496 659
Fragments arranged by size:
1496 702 659 602 333 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 820 850 820
Size: 30 4010
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 825 1036 825
Size: 211 3829
BseYI C'CCAG_C
Cuts at: 490 810 1465 2223 2800 3296 490
Size: 320 655 758 577 496 1234
Fragments arranged by size:
1234 758 655 577 496 320
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 487
Size: 4040
BsiEI CG_ry'CG
Cuts at: 104 155 1582 1628 2836 104
Size: 51 1427 46 1208 1308
Fragments arranged by size:
1427 1308 1208 51 46
BsiHKAI G_wGCw'C
Cuts at: 115 209 1054 1832 2022 2814 115
Size: 94 845 778 190 792 1341
Fragments arranged by size:
1341 845 792 778 190 94
BsiWI C'GTAC_G
Cuts at: 140 140
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 115 136 209 319 622 742 957 967 1054
Size: 21 73 110 303 120 215 10 87
Cuts at: 1054 1665 1758 1832 2022 2084 2814 115
Size: 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 303 215 190 120 110
93 87 74 73 62 21 10
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 296 3216 296
Size: 2920 1120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 453 459 1090 1304 1606 1987 453
Size: 6 631 214 302 381 2506
Fragments arranged by size:
2506 631 381 302 214 6
BsrBI CCG'CTC
Cuts at: 50 338 521 561 881 2332 2386 3870 4035
Size: 288 183 40 320 1451 54 1484 165
Cuts at: 4035 50
Size: 55
Fragments arranged by size:
1484 1451 320 288 183 165 55 54
40
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 167 232 902 967 1027 2038 2219 2428 167
Size: 65 670 65 60 1011 181 209 1779
Fragments arranged by size:
1779 1011 670 209 181 65 65 60
BssSI C'ACGA_G
Cuts at: 663 2311 2669 663
Size: 1648 358 2034
Fragments arranged by size:
2034 1648 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 762 762
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 3148 3234 3246
Size: 1064 467 23 335 246 1012 86 12
Cuts at: 3246 3592 1
Size: 346 449
Fragments arranged by size:
1064 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 389 707 389
Size: 318 3722
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 535 1746 2604 535
Size: 1211 858 1971
Fragments arranged by size:
1971 1211 858
EaeI y'GGCC_r
Cuts at: 152 591 882 944 1625 1799 2190 2217 3562
Size: 439 291 62 681 174 391 27 1345
Cuts at: 3562 152
Size: 630
Fragments arranged by size:
1345 681 630 439 391 291 174 62
27
EagI C'GGCC_G
Cuts at: 152 1625 152
Size: 1473 2567
EarI CTCTTCn'nnn_
Cuts at: 482 2063 2273 482
Size: 1581 210 2249
Fragments arranged by size:
2249 1581 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 207 1052 113
Size: 94 845 3101
Fragments arranged by size:
3101 845 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 715 1865 2297 3044 3312 289
Size: 426 1150 432 747 268 1017
Fragments arranged by size:
1150 1017 747 432 426 268
EcoNI CCTnn'n_nnAGG
Cuts at: 424 424
Size: 4040
EcoO109I rG'GnC_Cy
Cuts at: 132 133 264 782 993 1179 132
Size: 1 131 518 211 186 2993
Fragments arranged by size:
2993 518 211 186 131 1
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 872 1474 1722 2744 3723 872
Size: 602 248 1022 979 1189
Fragments arranged by size:
1189 1022 979 602 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 657 689 820 852 1882 1912 1914 1944 657
Size: 32 131 32 1030 30 2 30 2753
Fragments arranged by size:
2753 1030 131 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 868 1470 1718 868
Size: 602 248 3190
Fragments arranged by size:
3190 602 248
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 398 2711 2895 398
Size: 2313 184 1543
Fragments arranged by size:
2313 1543 184
MscI TGG'CCA
Cuts at: 593 1801 593
Size: 1208 2832
MslI CAynn'nnrTG
Cuts at: 295 677 797 2029 2156 295
Size: 382 120 1232 127 2179
Fragments arranged by size:
2179 1232 382 127 120
MspA1I CmG'CkG
Cuts at: 1151 1465 1825 2838 3083 1151
Size: 314 360 1013 245 2108
Fragments arranged by size:
2108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 904 1029 2221 904
Size: 125 1192 2723
Fragments arranged by size:
2723 1192 125
NarI GG'CG_CC
Cuts at: 869 1471 1719 869
Size: 602 248 3190
Fragments arranged by size:
3190 602 248
NcoI C'CATG_G
Cuts at: 389 707 389
Size: 318 3722
NgoMIV G'CCGG_C
Cuts at: 902 1027 2219 902
Size: 125 1192 2723
Fragments arranged by size:
2723 1192 125
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 782 993 782
Size: 211 3829
PspOMI G'GGCC_C
Cuts at: 132 132
Size: 4040
PstI C_TGCA'G
Cuts at: 518 614 851 1087 1772 518
Size: 96 237 236 685 2786
Fragments arranged by size:
2786 685 237 236 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 1465 1825 1465
Size: 360 3680
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 115 209 1054 115
Size: 94 845 3101
Fragments arranged by size:
3101 845 94
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SanDI GG'GwC_CC
Cuts at: 782 782
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 544 925 3557 544
Size: 381 2632 1027
Fragments arranged by size:
2632 1027 381
SfcI C'TryA_G
Cuts at: 33 307 514 610 847 1083 1768 2761 2952
Size: 274 207 96 237 236 685 993 191
Cuts at: 2952 33
Size: 1121
Fragments arranged by size:
1121 993 685 274 237 236 207 191
96
SfoI GGC'GCC
Cuts at: 870 1472 1720 870
Size: 602 248 3190
Fragments arranged by size:
3190 602 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SgrAI Cr'CCGG_yG
Cuts at: 967 967
Size: 4040
SmaI CCC'GGG
Cuts at: 780 986 1062 780
Size: 206 76 3758
Fragments arranged by size:
3758 206 76
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 389 707 1174 1528 389
Size: 318 467 354 2901
Fragments arranged by size:
2901 467 354 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 542 923 3555 542
Size: 381 2632 1027
Fragments arranged by size:
2632 1027 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 778 984 1060 778
Size: 206 76 3758
Fragments arranged by size:
3758 206 76
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI