Restriction map for
pF1KE6507
(Circular) (Six-base) MAPSORT of: pF1KE6507.seq Check: 4701 from: 1 to: 3743
pF1KE6507 3743 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 2000 2747 3015 1568
Size: 432 747 268 2296
Fragments arranged by size:
2296 747 432 268
AflIII A'CryG_T
Cuts at: 461 1095 2199 3176 461
Size: 634 1104 977 1028
Fragments arranged by size:
1104 1028 977 634
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AhdI GACnn_n'nnGTC
Cuts at: 645 645
Size: 3743
AlwNI CAG_nnn'CTG
Cuts at: 1048 2615 1048
Size: 1567 2176
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 336 747 3096 3352 336
Size: 411 2349 256 727
Fragments arranged by size:
2349 727 411 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 118 182 763 118
Size: 64 581 3098
Fragments arranged by size:
3098 581 64
BamHI G'GATC_C
Cuts at: 768 768
Size: 3743
BanI G'GyrC_C
Cuts at: 122 759 1173 1421 1456 122
Size: 637 414 248 35 2409
Fragments arranged by size:
2409 637 414 248 35
BanII G_rGCy'C
Cuts at: 677 757 1787 677
Size: 80 1030 2633
Fragments arranged by size:
2633 1030 80
BbeI G_GCGC'C
Cuts at: 126 1177 1425 126
Size: 1051 248 2444
Fragments arranged by size:
2444 1051 248
BbsI GAAGACnn'nnnn_
Cuts at: 446 701 446
Size: 255 3488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 225 229 259 263 225
Size: 4 30 4 3705
Fragments arranged by size:
3705 30 4 4
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2402 3743 1641
Size: 761 1341 1641
Fragments arranged by size:
1641 1341 761
BclI T'GATC_A
Cuts at: 539 1263 539
Size: 724 3019
BglI GCCn_nnn'nGGC
Cuts at: 227 368 227
Size: 141 3602
BglII A'GATC_T
Cuts at: 522 1258 3295 522
Size: 736 2037 970
Fragments arranged by size:
2037 970 736
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 1368 1461 2517 1368
Size: 93 1056 2594
Fragments arranged by size:
2594 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 523 623 633 955 1036 1371 3515 523
Size: 100 10 322 81 335 2144 751
Fragments arranged by size:
2144 751 335 322 100 81 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 3743
Bpu10I CC'TnA_GC
Cuts at: 596 596
Size: 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1798 2290 2588 919
Size: 879 492 298 2074
Fragments arranged by size:
2074 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 3447 376
Size: 3071 672
BsaAI yAC'GTr
Cuts at: 1096 1096
Size: 3743
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 123 148 553 1174 1422 2124 3620 123
Size: 25 405 621 248 702 1496 246
Fragments arranged by size:
1496 702 621 405 248 246 25
BsaWI w'CCGG_w
Cuts at: 724 932 1453 2131 2405 2552 2963 724
Size: 208 521 678 274 147 411 1504
Fragments arranged by size:
1504 678 521 411 274 208 147
BseYI C'CCAG_C
Cuts at: 154 1168 1926 2503 2999 154
Size: 1014 758 577 496 898
Fragments arranged by size:
1014 898 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 240
Size: 3743
BsiEI CG_ry'CG
Cuts at: 104 196 222 1285 1331 2539 104
Size: 92 26 1063 46 1208 1308
Fragments arranged by size:
1308 1208 1063 92 46 26
BsiHKAI G_wGCw'C
Cuts at: 677 757 1535 1725 2517 677
Size: 80 778 190 792 1903
Fragments arranged by size:
1903 792 778 190 80
Bsp1286I G_dGCh'C
Cuts at: 677 757 1368 1461 1535 1725 1787 2517 677
Size: 80 611 93 74 190 62 730 1903
Fragments arranged by size:
1903 730 611 190 93 80 74 62
BspEI T'CCGG_A
Cuts at: 724 2963 724
Size: 2239 1504
BspHI T'CATG_A
Cuts at: 2919 2919
Size: 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 262 793 1007 1309 1690 262
Size: 531 214 302 381 2315
Fragments arranged by size:
2315 531 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3573 3738 50
Size: 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1655 1655
Size: 3743
BsrFI r'CCGG_y
Cuts at: 136 195 1741 1922 2131 136
Size: 59 1546 181 209 1748
Fragments arranged by size:
1748 1546 209 181 59
BssSI C'ACGA_G
Cuts at: 2014 2372 2014
Size: 358 3385
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3743
BstBI TT'CG_AA
Cuts at: 277 2104 277
Size: 1827 1916
BstYI r'GATC_y
Cuts at: 1 522 768 1235 1258 1593 1839 2851 2937
Size: 521 246 467 23 335 246 1012 86
Cuts at: 2937 2949 3295 1
Size: 12 346 449
Fragments arranged by size:
1012 521 467 449 346 335 246 246
86 23 12
BtgI C'CryG_G
Cuts at: 190 225 190
Size: 35 3708
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1693 1874 2037 1693
Size: 181 163 3399
Fragments arranged by size:
3399 181 163
BtsI GCAGTG_nn'
Cuts at: 986 986
Size: 3743
ClaI AT'CG_AT
Cuts at: 245 245
Size: 3743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 498 533 498
Size: 35 3708
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 478 1449 2307 478
Size: 971 858 1914
Fragments arranged by size:
1914 971 858
EaeI y'GGCC_r
Cuts at: 193 219 665 1328 1502 1893 1920 3265 193
Size: 26 446 663 174 391 27 1345 671
Fragments arranged by size:
1345 671 663 446 391 174 27 26
EagI C'GGCC_G
Cuts at: 193 219 1328 193
Size: 26 1109 2608
Fragments arranged by size:
2608 1109 26
EarI CTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1951 2261 2407 3553 1951
Size: 310 146 1146 2141
Fragments arranged by size:
2141 1146 310 146
EcoICRI GAG'CTC
Cuts at: 675 755 675
Size: 80 3663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 2000 2747 3015 1568
Size: 432 747 268 2296
Fragments arranged by size:
2296 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 186 882 186
Size: 696 3047
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3743
FspI TGC'GCA
Cuts at: 235 1524 235
Size: 1289 2454
HaeII r_GCGC'y
Cuts at: 126 1177 1425 2447 3426 126
Size: 1051 248 1022 979 443
Fragments arranged by size:
1051 1022 979 443 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 631 663 1585 1615 1617 1647 631
Size: 32 922 30 2 30 2727
Fragments arranged by size:
2727 922 32 30 30 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3743
KasI G'GCGC_C
Cuts at: 122 1173 1421 122
Size: 1051 248 2444
Fragments arranged by size:
2444 1051 248
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2598 2414
Size: 184 3559
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 1732 1859 1732
Size: 127 3616
MspA1I CmG'CkG
Cuts at: 192 854 1168 1528 2541 2786 192
Size: 662 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 662 360 314 245
NaeI GCC'GGC
Cuts at: 138 197 1924 138
Size: 59 1727 1957
Fragments arranged by size:
1957 1727 59
NarI GG'CG_CC
Cuts at: 123 1174 1422 123
Size: 1051 248 2444
Fragments arranged by size:
2444 1051 248
NgoMIV G'CCGG_C
Cuts at: 136 195 1922 136
Size: 59 1727 1957
Fragments arranged by size:
1957 1727 59
NotI GC'GGCC_GC
Cuts at: 219 219
Size: 3743
NspI r_CATG'y
Cuts at: 796 1296 2203 796
Size: 500 907 2336
Fragments arranged by size:
2336 907 500
PciI A'CATG_T
Cuts at: 2199 2199
Size: 3743
PflMI CCAn_nnn'nTGG
Cuts at: 589 589
Size: 3743
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3711
PpuMI rG'GwC_Cy
Cuts at: 186 186
Size: 3743
PstI C_TGCA'G
Cuts at: 790 1475 790
Size: 685 3058
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 1168 1528 1168
Size: 360 3383
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 677 757 677
Size: 80 3663
SacII CC_GC'GG
Cuts at: 193 193
Size: 3743
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SanDI GG'GwC_CC
Cuts at: 186 186
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SfcI C'TryA_G
Cuts at: 33 786 1471 2464 2655 33
Size: 753 685 993 191 1121
Fragments arranged by size:
1121 993 753 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 227 227
Size: 3743
SfoI GGC'GCC
Cuts at: 124 1175 1423 124
Size: 1051 248 2444
Fragments arranged by size:
2444 1051 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 184 765 184
Size: 581 3162
SmlI C'TyrA_G
Cuts at: 898 1813 2305 2567 898
Size: 915 492 262 2074
Fragments arranged by size:
2074 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 796 1296 796
Size: 500 3243
StyI C'CwwG_G
Cuts at: 713 877 1231 713
Size: 164 354 3225
Fragments arranged by size:
3225 354 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 168 380 505 615 1326 1459 1918 2127 168
Size: 212 125 110 711 133 459 209 1784
Fragments arranged by size:
1784 711 459 212 209 133 125 110
TatI w'GTAC_w
Cuts at: 629 3258 629
Size: 2629 1114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 521 553 3260 3292 521
Size: 32 2707 32 972
Fragments arranged by size:
2707 972 32 32
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 375 774 375
Size: 399 3344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1238 1238
Size: 3743
XmaI C'CCGG_G
Cuts at: 182 763 182
Size: 581 3162
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI