Restriction map for
pF1KE5816
(Circular) (Six-base) MAPSORT of: pF1KE5816.seq Check: 6470 from: 1 to: 5699
pF1KE5816 5699 bp
With 182 enzymes: SgfI *
July 26, 2013 11:23 ..
AccI GT'mk_AC
Cuts at: 2737 2737
Size: 5699
Acc65I G'GTAC_C
Cuts at: 2715 2715
Size: 5699
AclI AA'CG_TT
Cuts at: 5539 5539
Size: 5699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 726 1377 1609 1691 2021 2554 3524 3956
Size: 305 651 232 82 330 533 970 432
Cuts at: 3956 4703 4971 421
Size: 747 268 1149
Fragments arranged by size:
1149 970 747 651 533 432 330 305
268 232 82
AfeI AGC'GCT
Cuts at: 244 244
Size: 5699
AflIII A'CryG_T
Cuts at: 200 3051 4155 5132 200
Size: 2851 1104 977 767
Fragments arranged by size:
2851 1104 977 767
AgeI A'CCGG_T
Cuts at: 4087 4087
Size: 5699
AleI CACnn'nnGTG
Cuts at: 2086 2086
Size: 5699
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2155 2187 2155
Size: 32 5667
AlwNI CAG_nnn'CTG
Cuts at: 516 543 3004 4571 516
Size: 27 2461 1567 1644
Fragments arranged by size:
2461 1644 1567 27
ApaI G_GGCC'C
Cuts at: 1808 1808
Size: 5699
ApaLI G'TGCA_C
Cuts at: 646 714 4469 646
Size: 68 3755 1876
Fragments arranged by size:
3755 1876 68
ApoI r'AATT_y
Cuts at: 880 1159 2035 2355 2362 2397 2703 5052 5308
Size: 279 876 320 7 35 306 2349 256
Cuts at: 5308 880
Size: 1271
Fragments arranged by size:
2349 1271 876 320 306 279 256 35
7
AseI AT'TA_AT
Cuts at: 20 20
Size: 5699
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
AvaI C'yCGr_G
Cuts at: 232 2177 2719 232
Size: 1945 542 3212
Fragments arranged by size:
3212 1945 542
BamHI G'GATC_C
Cuts at: 189 2724 189
Size: 2535 3164
BanI G'GyrC_C
Cuts at: 217 667 1924 2715 3129 3377 3412 217
Size: 450 1257 791 414 248 35 2504
Fragments arranged by size:
2504 1257 791 450 414 248 35
BanII G_rGCy'C
Cuts at: 842 1258 1736 1808 2713 3743 842
Size: 416 478 72 905 1030 2798
Fragments arranged by size:
2798 1030 905 478 416 72
BbeI G_GCGC'C
Cuts at: 671 3133 3381 671
Size: 2462 248 2989
Fragments arranged by size:
2989 2462 248
BbsI GAAGACnn'nnnn_
Cuts at: 821 2377 821
Size: 1556 4143
BbvCI CC'TCA_GC
Cuts at: 157 157
Size: 5699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 544 578 544
Size: 34 5665
BciVI GTATCCnnnnn_n'
Cuts at: 3597 4358 5699 3597
Size: 761 1341 3597
Fragments arranged by size:
3597 1341 761
BclI T'GATC_A
Cuts at: 3219 3219
Size: 5699
BfrBI ATG'CAT
Cuts at: 1324 1324
Size: 5699
BglI GCCn_nnn'nGGC
Cuts at: 1600 1600
Size: 5699
BglII A'GATC_T
Cuts at: 1814 3214 5251 1814
Size: 1400 2037 2262
Fragments arranged by size:
2262 2037 1400
BlpI GC'TnA_GC
Cuts at: 523 841 1756 2811 523
Size: 318 915 1055 3411
Fragments arranged by size:
3411 1055 915 318
Bme1580I G_kGCm'C
Cuts at: 220 650 718 1808 3324 3417 4473 220
Size: 430 68 1090 1516 93 1056 1446
Fragments arranged by size:
1516 1446 1090 1056 430 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 2911 2992 3327 5471 2911
Size: 81 335 2144 3139
Fragments arranged by size:
3139 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1781 2203 4066 1781
Size: 422 1863 3414
Fragments arranged by size:
3414 1863 422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 856 2541 316
Size: 540 1685 3474
Fragments arranged by size:
3474 1685 540
Bpu10I CC'TnA_GC
Cuts at: 157 157
Size: 5699
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 2576 2599 2875 3754 4246 4544 646
Size: 1930 23 276 879 492 298 1801
Fragments arranged by size:
1930 1801 879 492 298 276 23
BsaI GGTCTCn'nnnn_
Cuts at: 5403 5403
Size: 5699
BsaAI yAC'GTr
Cuts at: 1506 1614 1626 3052 1506
Size: 108 12 1426 4153
Fragments arranged by size:
4153 1426 108 12
BsaBI GATnn'nnATC
Cuts at: 6 3213 5250 6
Size: 3207 2037 455
Fragments arranged by size:
3207 2037 455
BsaHI Gr'CG_yC
Cuts at: 229 668 3130 3378 4080 5576 229
Size: 439 2462 248 702 1496 352
Fragments arranged by size:
2462 1496 702 439 352 248
BsaWI w'CCGG_w
Cuts at: 284 2888 3409 4087 4361 4508 4919 284
Size: 2604 521 678 274 147 411 1064
Fragments arranged by size:
2604 1064 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 253 313 1266 1501 2161 250
Size: 3 60 953 235 660 3788
Fragments arranged by size:
3788 953 660 235 60 3
BseYI C'CCAG_C
Cuts at: 1938 3124 3882 4459 4955 1938
Size: 1186 758 577 496 2682
Fragments arranged by size:
2682 1186 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1248 1495 1627 1648 1248
Size: 247 132 21 5299
Fragments arranged by size:
5299 247 132 21
BsiEI CG_ry'CG
Cuts at: 104 3241 3287 4495 104
Size: 3137 46 1208 1308
Fragments arranged by size:
3137 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 377 650 718 842 2713 3491 3681 4473 377
Size: 273 68 124 1871 778 190 792 1603
Fragments arranged by size:
1871 1603 792 778 273 190 124 68
BsmI GAATG_Cn'
Cuts at: 1326 1474 1326
Size: 148 5551
Bsp1286I G_dGCh'C
Cuts at: 220 377 650 718 842 1258 1736 1808 2713
Size: 157 273 68 124 416 478 72 905
Cuts at: 2713 3324 3417 3491 3681 3743 4473 220
Size: 611 93 74 190 62 730 1446
Fragments arranged by size:
1446 905 730 611 478 416 273 190
157 124 93 74 72 68 62
BspEI T'CCGG_A
Cuts at: 284 4919 284
Size: 4635 1064
BspHI T'CATG_A
Cuts at: 1262 1886 2456 4875 1262
Size: 624 570 2419 2086
Fragments arranged by size:
2419 2086 624 570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1532 2232 2749 2963 3265 3646 1532
Size: 700 517 214 302 381 3585
Fragments arranged by size:
3585 700 517 381 302 214
BsrBI CCG'CTC
Cuts at: 50 681 3991 4045 5529 5694 50
Size: 631 3310 54 1484 165 55
Fragments arranged by size:
3310 1484 631 165 55 54
BsrDI GCAATG_nn'
Cuts at: 714 919 1769 3611 714
Size: 205 850 1842 2802
Fragments arranged by size:
2802 1842 850 205
BsrFI r'CCGG_y
Cuts at: 143 3697 3878 4087 143
Size: 3554 181 209 1755
Fragments arranged by size:
3554 1755 209 181
BssSI C'ACGA_G
Cuts at: 3970 4328 3970
Size: 358 5341
BstAPI GCAn_nnn'nTGC
Cuts at: 654 713 1101 3076 654
Size: 59 388 1975 3277
Fragments arranged by size:
3277 1975 388 59
BstBI TT'CG_AA
Cuts at: 4060 4060
Size: 5699
BstXI CCAn_nnnn'nTGG
Cuts at: 1554 1554
Size: 5699
BstYI r'GATC_y
Cuts at: 1 189 1814 2724 3191 3214 3549 3795 4807
Size: 188 1625 910 467 23 335 246 1012
Cuts at: 4807 4893 4905 5251 1
Size: 86 12 346 449
Fragments arranged by size:
1625 1012 910 467 449 346 335 246
188 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1249 2668 1249
Size: 1419 4280
BtgI C'CryG_G
Cuts at: 362 1495 1595 2084 362
Size: 1133 100 489 3977
Fragments arranged by size:
3977 1133 489 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3649 3830 3993 3649
Size: 181 163 5355
Fragments arranged by size:
5355 181 163
BtsI GCAGTG_nn'
Cuts at: 624 2942 624
Size: 2318 3381
DraI TTT'AAA
Cuts at: 980 1968 2698 980
Size: 988 730 3981
Fragments arranged by size:
3981 988 730
DrdI GACnn_nn'nnGTC
Cuts at: 3405 4263 3405
Size: 858 4841
EaeI y'GGCC_r
Cuts at: 359 443 3284 3458 3849 3876 5221 359
Size: 84 2841 174 391 27 1345 837
Fragments arranged by size:
2841 1345 837 391 174 84 27
EagI C'GGCC_G
Cuts at: 3284 3284
Size: 5699
EarI CTCTTCn'nnn_
Cuts at: 956 3722 3932 956
Size: 2766 210 2723
Fragments arranged by size:
2766 2723 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1649 3907 4217 4363 5509 1649
Size: 2258 310 146 1146 1839
Fragments arranged by size:
2258 1839 1146 310 146
EcoICRI GAG'CTC
Cuts at: 840 2711 840
Size: 1871 3828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 421 726 856 1377 1609 1691 2021 2541
Size: 105 305 130 521 232 82 330 520
Cuts at: 2541 2554 3524 3956 4703 4971 316
Size: 13 970 432 747 268 1044
Fragments arranged by size:
1044 970 747 521 520 432 330 305
268 232 130 105 82 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1686 1686
Size: 5699
EcoO109I rG'GnC_Cy
Cuts at: 1805 2838 1805
Size: 1033 4666
EcoRI G'AATT_C
Cuts at: 2355 2397 2703 2355
Size: 42 306 5351
Fragments arranged by size:
5351 306 42
EcoRV GAT'ATC
Cuts at: 1842 2553 1842
Size: 711 4988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1464 3718 1464
Size: 2254 3445
FspI TGC'GCA
Cuts at: 466 3480 466
Size: 3014 2685
FspAI rTGC'GCAy
Cuts at: 466 466
Size: 5699
HaeII r_GCGC'y
Cuts at: 144 246 516 671 3133 3381 4403 5382 144
Size: 102 270 155 2462 248 1022 979 461
Fragments arranged by size:
2462 1022 979 461 270 248 155 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2572 2604 3541 3571 3573 3603 2572
Size: 32 937 30 2 30 4668
Fragments arranged by size:
4668 937 32 30 30 2
HincII GTy'rAC
Cuts at: 1141 2738 1141
Size: 1597 4102
KasI G'GCGC_C
Cuts at: 667 3129 3377 667
Size: 2462 248 2989
Fragments arranged by size:
2989 2462 248
KpnI G_GTAC'C
Cuts at: 2719 2719
Size: 5699
MfeI C'AATT_G
Cuts at: 1099 1099
Size: 5699
MluI A'CGCG_T
Cuts at: 5132 5132
Size: 5699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1034 4370 4554 1034
Size: 3336 184 2179
Fragments arranged by size:
3336 2179 184
MscI TGG'CCA
Cuts at: 445 3460 445
Size: 3015 2684
MslI CAynn'nnrTG
Cuts at: 277 1552 2086 3688 3815 277
Size: 1275 534 1602 127 2161
Fragments arranged by size:
2161 1602 1275 534 127
MspA1I CmG'CkG
Cuts at: 357 531 729 1608 2810 3124 3484 4497 4742
Size: 174 198 879 1202 314 360 1013 245
Cuts at: 4742 357
Size: 1314
Fragments arranged by size:
1314 1202 1013 879 360 314 245 198
174
NaeI GCC'GGC
Cuts at: 145 3880 145
Size: 3735 1964
NarI GG'CG_CC
Cuts at: 668 3130 3378 668
Size: 2462 248 2989
Fragments arranged by size:
2989 2462 248
NcoI C'CATG_G
Cuts at: 2084 2084
Size: 5699
NgoMIV G'CCGG_C
Cuts at: 143 3878 143
Size: 3735 1964
NsiI A_TGCA'T
Cuts at: 1326 1326
Size: 5699
NspI r_CATG'y
Cuts at: 138 204 2109 2352 2752 3252 4159 138
Size: 66 1905 243 400 500 907 1678
Fragments arranged by size:
1905 1678 907 500 400 243 66
PciI A'CATG_T
Cuts at: 200 4155 200
Size: 3955 1744
PfoI T'CCnGG_A
Cuts at: 185 248 1417 185
Size: 63 1169 4467
Fragments arranged by size:
4467 1169 63
PmeI GTTT'AAAC
Cuts at: 2698 2698
Size: 5699
PmlI CAC'GTG
Cuts at: 1626 1626
Size: 5699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4838 4870 4838
Size: 32 5667
PsiI TTA'TAA
Cuts at: 2075 2075
Size: 5699
PspOMI G'GGCC_C
Cuts at: 1804 1804
Size: 5699
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2038 2070 2038
Size: 32 5667
PstI C_TGCA'G
Cuts at: 356 1814 2746 3431 356
Size: 1458 932 685 2624
Fragments arranged by size:
2624 1458 932 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5699
PvuII CAG'CTG
Cuts at: 357 1608 3124 3484 357
Size: 1251 1516 360 2572
Fragments arranged by size:
2572 1516 1251 360
RsrII CG'GwC_CG
Cuts at: 3894 3894
Size: 5699
SacI G_AGCT'C
Cuts at: 842 2713 842
Size: 1871 3828
SalI G'TCGA_C
Cuts at: 2736 2736
Size: 5699
SapI GCTCTTCn'nnn_
Cuts at: 3722 3932 3722
Size: 210 5489
SbfI CC_TGCA'GG
Cuts at: 2746 2746
Size: 5699
ScaI AGT'ACT
Cuts at: 5216 5216
Size: 5699
SfcI C'TryA_G
Cuts at: 33 352 1810 2742 3427 4420 4611 33
Size: 319 1458 932 685 993 191 1121
Fragments arranged by size:
1458 1121 993 932 685 319 191
SfoI GGC'GCC
Cuts at: 669 3131 3379 669
Size: 2462 248 2989
Fragments arranged by size:
2989 2462 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
SmaI CCC'GGG
Cuts at: 2721 2721
Size: 5699
SmlI C'TyrA_G
Cuts at: 661 2578 2591 2854 3769 4261 4523 661
Size: 1917 13 263 915 492 262 1837
Fragments arranged by size:
1917 1837 915 492 263 262 13
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5699
SphI G_CATG'C
Cuts at: 2352 2752 3252 2352
Size: 400 500 4799
Fragments arranged by size:
4799 500 400
StuI AGG'CCT
Cuts at: 1190 1190
Size: 5699
StyI C'CwwG_G
Cuts at: 2084 2833 3187 2084
Size: 749 354 4596
Fragments arranged by size:
4596 749 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1346 3282 3415 3874 4083 1346
Size: 1936 133 459 209 2962
Fragments arranged by size:
2962 1936 459 209 133
TatI w'GTAC_w
Cuts at: 1907 5214 1907
Size: 3307 2392
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1684 1716 5216 5248 1684
Size: 32 3500 32 2135
Fragments arranged by size:
3500 2135 32 32
Tth111I GACn'n_nGTC
Cuts at: 820 2515 3496 820
Size: 1695 981 3023
Fragments arranged by size:
3023 1695 981
XbaI T'CTAG_A
Cuts at: 2730 2730
Size: 5699
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3194 3194
Size: 5699
XmaI C'CCGG_G
Cuts at: 2719 2719
Size: 5699
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI
PmeI PmlI PpiI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BmgBI BmtI BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII FseI HindIII HpaI NdeI
NheI NotI NruI PacI PasI PflMI PpuMI PshAI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI