Restriction map for
pF1KE5788
(Circular) (Six-base) MAPSORT of: pF1KE5788.seq Check: 2963 from: 1 to: 5453
pF1KE5788 5453 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AatII G_ACGT'C
Cuts at: 118 1491 118
Size: 1373 4080
AccI GT'mk_AC
Cuts at: 2491 2491
Size: 5453
Acc65I G'GTAC_C
Cuts at: 2095 2469 2095
Size: 374 5079
AclI AA'CG_TT
Cuts at: 5293 5293
Size: 5453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 1409 1637 3278 3710 4457 4725 130
Size: 1279 228 1641 432 747 268 858
Fragments arranged by size:
1641 1279 858 747 432 268 228
AflII C'TTAA_G
Cuts at: 1429 1963 1429
Size: 534 4919
AflIII A'CryG_T
Cuts at: 1346 2339 2805 3909 4886 1346
Size: 993 466 1104 977 1913
Fragments arranged by size:
1913 1104 993 977 466
AgeI A'CCGG_T
Cuts at: 3841 3841
Size: 5453
AhdI GACnn_n'nnGTC
Cuts at: 1487 1487
Size: 5453
AlwNI CAG_nnn'CTG
Cuts at: 1096 2758 4325 1096
Size: 1662 1567 2224
Fragments arranged by size:
2224 1662 1567
ApaLI G'TGCA_C
Cuts at: 4223 4223
Size: 5453
ApoI r'AATT_y
Cuts at: 287 695 1694 1786 2327 2457 4806 5062 287
Size: 408 999 92 541 130 2349 256 678
Fragments arranged by size:
2349 999 678 541 408 256 130 92
AseI AT'TA_AT
Cuts at: 20 20
Size: 5453
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
AvaI C'yCGr_G
Cuts at: 2473 2473
Size: 5453
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1417 1450 1417
Size: 33 5420
BamHI G'GATC_C
Cuts at: 2478 2478
Size: 5453
BanI G'GyrC_C
Cuts at: 1708 2095 2469 2883 3131 3166 1708
Size: 387 374 414 248 35 3995
Fragments arranged by size:
3995 414 387 374 248 35
BanII G_rGCy'C
Cuts at: 2467 3497 2467
Size: 1030 4423
BbeI G_GCGC'C
Cuts at: 1712 2887 3135 1712
Size: 1175 248 4030
Fragments arranged by size:
4030 1175 248
BbsI GAAGACnn'nnnn_
Cuts at: 161 506 161
Size: 345 5108
BciVI GTATCCnnnnn_n'
Cuts at: 3351 4112 5453 3351
Size: 761 1341 3351
Fragments arranged by size:
3351 1341 761
BclI T'GATC_A
Cuts at: 959 2973 959
Size: 2014 3439
BglII A'GATC_T
Cuts at: 2968 5005 2968
Size: 2037 3416
BlpI GC'TnA_GC
Cuts at: 2303 2565 2303
Size: 262 5191
Bme1580I G_kGCm'C
Cuts at: 3078 3171 4227 3078
Size: 93 1056 4304
Fragments arranged by size:
4304 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2665 2746 3081 5225 2665
Size: 81 335 2144 2893
Fragments arranged by size:
2893 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3820 3820
Size: 5453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1780 1780
Size: 5453
Bpu10I CC'TnA_GC
Cuts at: 1322 1322
Size: 5453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 2048 2629 3508 4000 4298 337
Size: 1711 581 879 492 298 1492
Fragments arranged by size:
1711 1492 879 581 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5157 5157
Size: 5453
BsaAI yAC'GTr
Cuts at: 2806 2806
Size: 5453
BsaBI GATnn'nnATC
Cuts at: 6 2967 5004 6
Size: 2961 2037 455
Fragments arranged by size:
2961 2037 455
BsaHI Gr'CG_yC
Cuts at: 115 1488 1709 2884 3132 3834 5330 115
Size: 1373 221 1175 248 702 1496 238
Fragments arranged by size:
1496 1373 1175 702 248 238 221
BsaWI w'CCGG_w
Cuts at: 1773 2642 3163 3841 4115 4262 4673 1773
Size: 869 521 678 274 147 411 2553
Fragments arranged by size:
2553 869 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 279 2878 3636 4213 4709 279
Size: 2599 758 577 496 1023
Fragments arranged by size:
2599 1023 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2995 3041 4249 104
Size: 2891 46 1208 1308
Fragments arranged by size:
2891 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 843 1421 2396 2467 3245 3435 4227 843
Size: 578 975 71 778 190 792 2069
Fragments arranged by size:
2069 975 792 778 578 190 71
BsmI GAATG_Cn'
Cuts at: 901 1707 901
Size: 806 4647
Bsp1286I G_dGCh'C
Cuts at: 843 1421 2396 2467 3078 3171 3245 3435 3497
Size: 578 975 71 611 93 74 190 62
Cuts at: 3497 4227 843
Size: 730 2069
Fragments arranged by size:
2069 975 730 611 578 190 93 74
71 62
BspEI T'CCGG_A
Cuts at: 4673 4673
Size: 5453
BspHI T'CATG_A
Cuts at: 4629 4629
Size: 5453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2503 2717 3019 3400 2503
Size: 214 302 381 4556
Fragments arranged by size:
4556 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3745 3799 5283 5448 50
Size: 3695 54 1484 165 55
Fragments arranged by size:
3695 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 590 3365 590
Size: 2775 2678
BsrFI r'CCGG_y
Cuts at: 3451 3632 3841 3451
Size: 181 209 5063
Fragments arranged by size:
5063 209 181
BsrGI T'GTAC_A
Cuts at: 786 1437 786
Size: 651 4802
BssSI C'ACGA_G
Cuts at: 341 3724 4082 341
Size: 3383 358 1712
Fragments arranged by size:
3383 1712 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2830 2830
Size: 5453
BstBI TT'CG_AA
Cuts at: 513 3814 513
Size: 3301 2152
BstEII G'GTnAC_C
Cuts at: 1890 1890
Size: 5453
BstYI r'GATC_y
Cuts at: 1 2478 2945 2968 3303 3549 4561 4647 4659
Size: 2477 467 23 335 246 1012 86 12
Cuts at: 4659 5005 1
Size: 346 449
Fragments arranged by size:
2477 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 680 680
Size: 5453
BtgI C'CryG_G
Cuts at: 107 107
Size: 5453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3403 3584 3747 3403
Size: 181 163 5109
Fragments arranged by size:
5109 181 163
BtsI GCAGTG_nn'
Cuts at: 229 2696 229
Size: 2467 2986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2362 2397 2362
Size: 35 5418
DraI TTT'AAA
Cuts at: 302 927 1257 2452 302
Size: 625 330 1195 3303
Fragments arranged by size:
3303 1195 625 330
DraIII CAC_nnn'GTG
Cuts at: 418 418
Size: 5453
DrdI GACnn_nn'nnGTC
Cuts at: 2010 3159 4017 2010
Size: 1149 858 3446
Fragments arranged by size:
3446 1149 858
EaeI y'GGCC_r
Cuts at: 3038 3212 3603 3630 4975 3038
Size: 174 391 27 1345 3516
Fragments arranged by size:
3516 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3038 3038
Size: 5453
EarI CTCTTCn'nnn_
Cuts at: 149 1385 2107 2207 2403 3476 3686 149
Size: 1236 722 100 196 1073 210 1916
Fragments arranged by size:
1916 1236 1073 722 210 196 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3661 3971 4117 5263 3661
Size: 310 146 1146 3851
Fragments arranged by size:
3851 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2465 2465
Size: 5453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 1409 1637 1780 3278 3710 4457 4725 130
Size: 1279 228 143 1498 432 747 268 858
Fragments arranged by size:
1498 1279 858 747 432 268 228 143
EcoO109I rG'GnC_Cy
Cuts at: 2592 2592
Size: 5453
EcoRI G'AATT_C
Cuts at: 1786 2457 1786
Size: 671 4782
EcoRV GAT'ATC
Cuts at: 2424 2424
Size: 5453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 160 331 741 1423 3472 160
Size: 171 410 682 2049 2141
Fragments arranged by size:
2141 2049 682 410 171
FspI TGC'GCA
Cuts at: 3234 3234
Size: 5453
HaeII r_GCGC'y
Cuts at: 1712 2887 3135 4157 5136 1712
Size: 1175 248 1022 979 2029
Fragments arranged by size:
2029 1175 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 1473 1505 1931 1963 3295 3325 3327
Size: 32 1336 32 426 32 1332 30 2
Cuts at: 3327 3357 105
Size: 30 2201
Fragments arranged by size:
2201 1336 1332 426 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 445 663 2070 2492 445
Size: 218 1407 422 3406
Fragments arranged by size:
3406 1407 422 218
HindIII A'AGCT_T
Cuts at: 190 517 617 1178 1405 1582 1631 1966 190
Size: 327 100 561 227 177 49 335 3677
Fragments arranged by size:
3677 561 335 327 227 177 100 49
HpaI GTT'AAC
Cuts at: 663 663
Size: 5453
KasI G'GCGC_C
Cuts at: 1708 2883 3131 1708
Size: 1175 248 4030
Fragments arranged by size:
4030 1175 248
KpnI G_GTAC'C
Cuts at: 2099 2473 2099
Size: 374 5079
MluI A'CGCG_T
Cuts at: 4886 4886
Size: 5453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4124 4308 4124
Size: 184 5269
MscI TGG'CCA
Cuts at: 3214 3214
Size: 5453
MslI CAynn'nnrTG
Cuts at: 359 2440 3442 3569 359
Size: 2081 1002 127 2243
Fragments arranged by size:
2243 2081 1002 127
MspA1I CmG'CkG
Cuts at: 633 1093 1567 1675 2307 2564 2878 3238 4251
Size: 460 474 108 632 257 314 360 1013
Cuts at: 4251 4496 633
Size: 245 1590
Fragments arranged by size:
1590 1013 632 474 460 360 314 257
245 108
NaeI GCC'GGC
Cuts at: 3634 3634
Size: 5453
NarI GG'CG_CC
Cuts at: 1709 2884 3132 1709
Size: 1175 248 4030
Fragments arranged by size:
4030 1175 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5453
NgoMIV G'CCGG_C
Cuts at: 3632 3632
Size: 5453
NspI r_CATG'y
Cuts at: 253 601 1350 2343 2506 3006 3913 253
Size: 348 749 993 163 500 907 1793
Fragments arranged by size:
1793 993 907 749 500 348 163
PciI A'CATG_T
Cuts at: 1346 2339 3909 1346
Size: 993 1570 2890
Fragments arranged by size:
2890 1570 993
PmeI GTTT'AAAC
Cuts at: 2452 2452
Size: 5453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 4592
Size: 32 5421
PsiI TTA'TAA
Cuts at: 1852 1852
Size: 5453
PstI C_TGCA'G
Cuts at: 2312 2500 3185 2312
Size: 188 685 4580
Fragments arranged by size:
4580 685 188
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5453
PvuII CAG'CTG
Cuts at: 633 1093 1675 2307 2878 3238 633
Size: 460 582 632 571 360 2848
Fragments arranged by size:
2848 632 582 571 460 360
RsrII CG'GwC_CG
Cuts at: 3648 3648
Size: 5453
SacI G_AGCT'C
Cuts at: 2467 2467
Size: 5453
SalI G'TCGA_C
Cuts at: 2490 2490
Size: 5453
SapI GCTCTTCn'nnn_
Cuts at: 3476 3686 3476
Size: 210 5243
SbfI CC_TGCA'GG
Cuts at: 2500 2500
Size: 5453
ScaI AGT'ACT
Cuts at: 347 4970 347
Size: 4623 830
SfcI C'TryA_G
Cuts at: 33 2192 2308 2496 3181 4174 4365 33
Size: 2159 116 188 685 993 191 1121
Fragments arranged by size:
2159 1121 993 685 191 188 116
SfoI GGC'GCC
Cuts at: 1710 2885 3133 1710
Size: 1175 248 4030
Fragments arranged by size:
4030 1175 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
SmaI CCC'GGG
Cuts at: 2475 2475
Size: 5453
SmlI C'TyrA_G
Cuts at: 316 1429 1963 2063 2608 3523 4015 4277 316
Size: 1113 534 100 545 915 492 262 1492
Fragments arranged by size:
1492 1113 915 545 534 492 262 100
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5453
SphI G_CATG'C
Cuts at: 253 2506 3006 253
Size: 2253 500 2700
Fragments arranged by size:
2700 2253 500
StyI C'CwwG_G
Cuts at: 107 951 2587 2941 107
Size: 844 1636 354 2619
Fragments arranged by size:
2619 1636 844 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2091 3036 3169 3628 3837 2091
Size: 945 133 459 209 3707
Fragments arranged by size:
3707 945 459 209 133
TatI w'GTAC_w
Cuts at: 345 786 1437 4968 345
Size: 441 651 3531 830
Fragments arranged by size:
3531 830 651 441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4970 5002 4970
Size: 32 5421
Tth111I GACn'n_nGTC
Cuts at: 3250 3250
Size: 5453
XbaI T'CTAG_A
Cuts at: 2484 2484
Size: 5453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2948 2948
Size: 5453
XmaI C'CCGG_G
Cuts at: 2473 2473
Size: 5453
XmnI GAAnn'nnTTC
Cuts at: 311 1181 311
Size: 870 4583
ZraI GAC'GTC
Cuts at: 116 1489 116
Size: 1373 4080
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmBI BssHII BstXI BstZ17I ClaI EcoNI
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI