Restriction map for
pF1KE5665
(Circular) (Six-base) MAPSORT of: pF1KE5665.seq Check: 6528 from: 1 to: 4697
pF1KE5665 4697 bp
With 182 enzymes: SgfI *
October 12, 2018 16:45 ..
AccI GT'mk_AC
Cuts at: 1735 1735
Size: 4697
Acc65I G'GTAC_C
Cuts at: 1713 1713
Size: 4697
AclI AA'CG_TT
Cuts at: 4537 4537
Size: 4697
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2522 2954 3701 3969 2522
Size: 432 747 268 3250
Fragments arranged by size:
3250 747 432 268
AflIII A'CryG_T
Cuts at: 782 1328 1361 2049 3153 4130 782
Size: 546 33 688 1104 977 1349
Fragments arranged by size:
1349 1104 977 688 546 33
AgeI A'CCGG_T
Cuts at: 3085 3085
Size: 4697
AleI CACnn'nnGTG
Cuts at: 613 890 613
Size: 277 4420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 908 1646 908
Size: 738 3959
AlwNI CAG_nnn'CTG
Cuts at: 679 808 1045 2002 3569 679
Size: 129 237 957 1567 1807
Fragments arranged by size:
1807 1567 957 237 129
ApaI G_GGCC'C
Cuts at: 119 119
Size: 4697
ApaLI G'TGCA_C
Cuts at: 3467 3467
Size: 4697
ApoI r'AATT_y
Cuts at: 1701 4050 4306 1701
Size: 2349 256 2092
Fragments arranged by size:
2349 2092 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4697
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4697
AvaI C'yCGr_G
Cuts at: 1353 1717 1353
Size: 364 4333
BamHI G'GATC_C
Cuts at: 323 1722 323
Size: 1399 3298
BanI G'GyrC_C
Cuts at: 300 429 637 710 892 1437 1593 1713 2127
Size: 129 208 73 182 545 156 120 414
Cuts at: 2127 2375 2410 300
Size: 248 35 2587
Fragments arranged by size:
2587 545 414 248 208 182 156 129
120 73 35
BanII G_rGCy'C
Cuts at: 119 923 977 1282 1414 1574 1711 2741 119
Size: 804 54 305 132 160 137 1030 2075
Fragments arranged by size:
2075 1030 804 305 160 137 132 54
BbeI G_GCGC'C
Cuts at: 1597 2131 2379 1597
Size: 534 248 3915
Fragments arranged by size:
3915 534 248
BbsI GAAGACnn'nnnn_
Cuts at: 1239 1475 1239
Size: 236 4461
BbvCI CC'TCA_GC
Cuts at: 176 215 693 176
Size: 39 478 4180
Fragments arranged by size:
4180 478 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4663
BciVI GTATCCnnnnn_n'
Cuts at: 2595 3356 4697 2595
Size: 761 1341 2595
Fragments arranged by size:
2595 1341 761
BclI T'GATC_A
Cuts at: 2217 2217
Size: 4697
BglII A'GATC_T
Cuts at: 2212 4249 2212
Size: 2037 2660
BlpI GC'TnA_GC
Cuts at: 1060 1809 1060
Size: 749 3948
Bme1580I G_kGCm'C
Cuts at: 119 305 1442 2322 2415 3471 119
Size: 186 1137 880 93 1056 1345
Fragments arranged by size:
1345 1137 1056 880 186 93
BmgBI CAC'GTC
Cuts at: 756 1331 1389 1473 756
Size: 575 58 84 3980
Fragments arranged by size:
3980 575 84 58
BmrI ACTGGGnnnn_n'
Cuts at: 361 1621 1909 1990 2325 4469 361
Size: 1260 288 81 335 2144 589
Fragments arranged by size:
2144 1260 589 335 288 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 570 3064 570
Size: 2494 2203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 1588
Size: 4697
Bpu10I CC'TnA_GC
Cuts at: 176 215 693 1310 176
Size: 39 478 617 3563
Fragments arranged by size:
3563 617 478 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 1873 2752 3244 3542 519
Size: 1354 879 492 298 1674
Fragments arranged by size:
1674 1354 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 998 1447 4401 998
Size: 449 2954 1294
Fragments arranged by size:
2954 1294 449
BsaAI yAC'GTr
Cuts at: 1362 2050 1362
Size: 688 4009
BsaBI GATnn'nnATC
Cuts at: 6 2211 4248 6
Size: 2205 2037 455
Fragments arranged by size:
2205 2037 455
BsaHI Gr'CG_yC
Cuts at: 164 364 931 1172 1594 1616 1678 2128 2376
Size: 200 567 241 422 22 62 450 248
Cuts at: 2376 3078 4574 164
Size: 702 1496 287
Fragments arranged by size:
1496 702 567 450 422 287 248 241
200 62 22
BsaWI w'CCGG_w
Cuts at: 1886 2407 3085 3359 3506 3917 1886
Size: 521 678 274 147 411 2666
Fragments arranged by size:
2666 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 196 1219 193
Size: 3 1023 3671
Fragments arranged by size:
3671 1023 3
BseYI C'CCAG_C
Cuts at: 135 171 816 1300 1671 2122 2880 3457 3953
Size: 36 645 484 371 451 758 577 496
Cuts at: 3953 135
Size: 879
Fragments arranged by size:
879 758 645 577 496 484 451 371
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 637 886 1540 1590 524
Size: 113 249 654 50 3631
Fragments arranged by size:
3631 654 249 113 50
BsiEI CG_ry'CG
Cuts at: 104 1104 2239 2285 3493 104
Size: 1000 1135 46 1208 1308
Fragments arranged by size:
1308 1208 1135 1000 46
BsiHKAI G_wGCw'C
Cuts at: 923 1282 1711 2489 2679 3471 923
Size: 359 429 778 190 792 2149
Fragments arranged by size:
2149 792 778 429 359 190
BsmBI CGTCTCn'nnnn_
Cuts at: 355 355
Size: 4697
Bsp1286I G_dGCh'C
Cuts at: 119 305 923 977 1282 1414 1442 1574 1711
Size: 186 618 54 305 132 28 132 137
Cuts at: 1711 2322 2415 2489 2679 2741 3471 119
Size: 611 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 618 611 305 190 186 137
132 132 93 74 62 54 28
BspEI T'CCGG_A
Cuts at: 3917 3917
Size: 4697
BspHI T'CATG_A
Cuts at: 1122 3873 1122
Size: 2751 1946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1747 1961 2263 2644 1747
Size: 214 302 381 3800
Fragments arranged by size:
3800 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1406 2989 3043 4527 4692 50
Size: 1356 1583 54 1484 165 55
Fragments arranged by size:
1583 1484 1356 165 55 54
BsrDI GCAATG_nn'
Cuts at: 549 1353 2609 549
Size: 804 1256 2637
Fragments arranged by size:
2637 1256 804
BsrFI r'CCGG_y
Cuts at: 634 2695 2876 3085 634
Size: 2061 181 209 2246
Fragments arranged by size:
2246 2061 209 181
BssHII G'CGCG_C
Cuts at: 1486 1486
Size: 4697
BssSI C'ACGA_G
Cuts at: 2968 3326 2968
Size: 358 4339
BstAPI GCAn_nnn'nTGC
Cuts at: 1509 1518 2074 1509
Size: 9 556 4132
Fragments arranged by size:
4132 556 9
BstBI TT'CG_AA
Cuts at: 3058 3058
Size: 4697
BstEII G'GTnAC_C
Cuts at: 600 600
Size: 4697
BstYI r'GATC_y
Cuts at: 1 323 995 1722 2189 2212 2547 2793 3805
Size: 322 672 727 467 23 335 246 1012
Cuts at: 3805 3891 3903 4249 1
Size: 86 12 346 449
Fragments arranged by size:
1012 727 672 467 449 346 335 322
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 142 142
Size: 4697
BtgI C'CryG_G
Cuts at: 305 374 452 791 1074 1113 1458 305
Size: 69 78 339 283 39 345 3544
Fragments arranged by size:
3544 345 339 283 78 69 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 302 705 2647 2828 2991 302
Size: 403 1942 181 163 2008
Fragments arranged by size:
2008 1942 403 181 163
BtsI GCAGTG_nn'
Cuts at: 1645 1940 1645
Size: 295 4402
DraI TTT'AAA
Cuts at: 1696 1696
Size: 4697
DraIII CAC_nnn'GTG
Cuts at: 654 1509 654
Size: 855 3842
DrdI GACnn_nn'nnGTC
Cuts at: 2403 3261 2403
Size: 858 3839
EaeI y'GGCC_r
Cuts at: 371 1101 1652 2282 2456 2847 2874 4219 371
Size: 730 551 630 174 391 27 1345 849
Fragments arranged by size:
1345 849 730 630 551 391 174 27
EagI C'GGCC_G
Cuts at: 1101 2282 1101
Size: 1181 3516
EarI CTCTTCn'nnn_
Cuts at: 283 2720 2930 283
Size: 2437 210 2050
Fragments arranged by size:
2437 2050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 782 2905 3215 3361 4507 782
Size: 2123 310 146 1146 972
Fragments arranged by size:
2123 1146 972 310 146
EcoICRI GAG'CTC
Cuts at: 921 1280 1709 921
Size: 359 429 3909
Fragments arranged by size:
3909 429 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 2522 2954 3701 3969 1588
Size: 934 432 747 268 2316
Fragments arranged by size:
2316 934 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 865 865
Size: 4697
EcoO109I rG'GnC_Cy
Cuts at: 115 116 219 355 797 1658 1836 115
Size: 1 103 136 442 861 178 2976
Fragments arranged by size:
2976 861 442 178 136 103 1
EcoRI G'AATT_C
Cuts at: 1701 1701
Size: 4697
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 498 2716 498
Size: 2218 2479
FspI TGC'GCA
Cuts at: 2478 2478
Size: 4697
HaeII r_GCGC'y
Cuts at: 1597 2131 2379 3401 4380 1597
Size: 534 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 534 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2539 2569 2571 2601 2539
Size: 30 2 30 4635
Fragments arranged by size:
4635 30 30 2
HincII GTy'rAC
Cuts at: 1736 1736
Size: 4697
KasI G'GCGC_C
Cuts at: 1593 2127 2375 1593
Size: 534 248 3915
Fragments arranged by size:
3915 534 248
KpnI G_GTAC'C
Cuts at: 1717 1717
Size: 4697
MluI A'CGCG_T
Cuts at: 4130 4130
Size: 4697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 764 3368 3552 764
Size: 2604 184 1909
Fragments arranged by size:
2604 1909 184
MscI TGG'CCA
Cuts at: 373 2458 373
Size: 2085 2612
MslI CAynn'nnrTG
Cuts at: 556 613 754 890 1127 1241 2686 2813 556
Size: 57 141 136 237 114 1445 127 2440
Fragments arranged by size:
2440 1445 237 141 136 127 114 57
MspA1I CmG'CkG
Cuts at: 175 214 820 1076 1231 1684 1808 2122 2482
Size: 39 606 256 155 453 124 314 360
Cuts at: 2482 3495 3740 175
Size: 1013 245 1132
Fragments arranged by size:
1132 1013 606 453 360 314 256 245
155 124 39
NaeI GCC'GGC
Cuts at: 636 2878 636
Size: 2242 2455
NarI GG'CG_CC
Cuts at: 1594 2128 2376 1594
Size: 534 248 3915
Fragments arranged by size:
3915 534 248
NcoI C'CATG_G
Cuts at: 374 791 1113 374
Size: 417 322 3958
Fragments arranged by size:
3958 417 322
NgoMIV G'CCGG_C
Cuts at: 634 2876 634
Size: 2242 2455
NotI GC'GGCC_GC
Cuts at: 1101 1101
Size: 4697
NspI r_CATG'y
Cuts at: 687 786 1546 1750 2250 3157 687
Size: 99 760 204 500 907 2227
Fragments arranged by size:
2227 907 760 500 204 99
PasI CC'CwG_GG
Cuts at: 119 159 119
Size: 40 4657
PciI A'CATG_T
Cuts at: 782 3153 782
Size: 2371 2326
PflMI CCAn_nnn'nTGG
Cuts at: 960 960
Size: 4697
PmeI GTTT'AAAC
Cuts at: 1696 1696
Size: 4697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 4665
PpuMI rG'GwC_Cy
Cuts at: 797 1658 797
Size: 861 3836
PshAI GACnn'nnGTC
Cuts at: 1115 1222 1115
Size: 107 4590
PspOMI G'GGCC_C
Cuts at: 115 115
Size: 4697
PstI C_TGCA'G
Cuts at: 245 1744 2429 245
Size: 1499 685 2513
Fragments arranged by size:
2513 1499 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4697
PvuII CAG'CTG
Cuts at: 175 214 820 2122 2482 175
Size: 39 606 1302 360 2390
Fragments arranged by size:
2390 1302 606 360 39
RsrII CG'GwC_CG
Cuts at: 2892 2892
Size: 4697
SacI G_AGCT'C
Cuts at: 923 1282 1711 923
Size: 359 429 3909
Fragments arranged by size:
3909 429 359
SacII CC_GC'GG
Cuts at: 1077 1077
Size: 4697
SalI G'TCGA_C
Cuts at: 1734 1734
Size: 4697
SanDI GG'GwC_CC
Cuts at: 797 1658 797
Size: 861 3836
SapI GCTCTTCn'nnn_
Cuts at: 2720 2930 2720
Size: 210 4487
SbfI CC_TGCA'GG
Cuts at: 1744 1744
Size: 4697
ScaI AGT'ACT
Cuts at: 4214 4214
Size: 4697
SexAI A'CCwGG_T
Cuts at: 860 1505 860
Size: 645 4052
SfcI C'TryA_G
Cuts at: 33 148 241 1740 2425 3418 3609 33
Size: 115 93 1499 685 993 191 1121
Fragments arranged by size:
1499 1121 993 685 191 115 93
SfoI GGC'GCC
Cuts at: 1595 2129 2377 1595
Size: 534 248 3915
Fragments arranged by size:
3915 534 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4697
SmaI CCC'GGG
Cuts at: 1355 1719 1355
Size: 364 4333
SmlI C'TyrA_G
Cuts at: 498 1852 2767 3259 3521 498
Size: 1354 915 492 262 1674
Fragments arranged by size:
1674 1354 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4697
SphI G_CATG'C
Cuts at: 1750 2250 1750
Size: 500 4197
SrfI GCCC'GGGC
Cuts at: 1355 1355
Size: 4697
StyI C'CwwG_G
Cuts at: 374 762 791 1113 1831 2185 374
Size: 388 29 322 718 354 2886
Fragments arranged by size:
2886 718 388 354 322 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 111 1640 2280 2413 2872 3081 111
Size: 1529 640 133 459 209 1727
Fragments arranged by size:
1727 1529 640 459 209 133
TatI w'GTAC_w
Cuts at: 779 4212 779
Size: 3433 1264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4214 4246 4214
Size: 32 4665
Tth111I GACn'n_nGTC
Cuts at: 2494 2494
Size: 4697
XbaI T'CTAG_A
Cuts at: 1728 1728
Size: 4697
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1290 2192 1290
Size: 902 3795
XmaI C'CCGG_G
Cuts at: 1353 1717 1353
Size: 364 4333
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BfrBI BglI BmtI BsaXI BsiWI BsmI BsrGI
BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PfoI
PmlI PsiI PspXI PsrI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI