Restriction map for
pF1KE5606
(Circular) (Six-base) MAPSORT of: pF1KE5606.seq Check: 6261 from: 1 to: 4646
pF1KE5606 4646 bp
With 182 enzymes: SgfI *
February 27, 2017 10:28 ..
AccI GT'mk_AC
Cuts at: 1684 1684
Size: 4646
Acc65I G'GTAC_C
Cuts at: 1662 1662
Size: 4646
AclI AA'CG_TT
Cuts at: 4486 4486
Size: 4646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1622 2471 2903 3650 3918 1622
Size: 849 432 747 268 2350
Fragments arranged by size:
2350 849 747 432 268
AfeI AGC'GCT
Cuts at: 1028 1028
Size: 4646
AflIII A'CryG_T
Cuts at: 755 1998 3102 4079 755
Size: 1243 1104 977 1322
Fragments arranged by size:
1322 1243 1104 977
AgeI A'CCGG_T
Cuts at: 3034 3034
Size: 4646
AlwNI CAG_nnn'CTG
Cuts at: 494 812 1951 3518 494
Size: 318 1139 1567 1622
Fragments arranged by size:
1622 1567 1139 318
ApaLI G'TGCA_C
Cuts at: 3416 3416
Size: 4646
ApoI r'AATT_y
Cuts at: 385 916 1550 1650 3999 4255 385
Size: 531 634 100 2349 256 776
Fragments arranged by size:
2349 776 634 531 256 100
AseI AT'TA_AT
Cuts at: 20 449 1268 1526 20
Size: 429 819 258 3140
Fragments arranged by size:
3140 819 429 258
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
AvaI C'yCGr_G
Cuts at: 1666 1666
Size: 4646
BamHI G'GATC_C
Cuts at: 1671 1671
Size: 4646
BanI G'GyrC_C
Cuts at: 1316 1662 2076 2324 2359 1316
Size: 346 414 248 35 3603
Fragments arranged by size:
3603 414 346 248 35
BanII G_rGCy'C
Cuts at: 153 1660 2690 153
Size: 1507 1030 2109
Fragments arranged by size:
2109 1507 1030
BbeI G_GCGC'C
Cuts at: 1320 2080 2328 1320
Size: 760 248 3638
Fragments arranged by size:
3638 760 248
BbsI GAAGACnn'nnnn_
Cuts at: 637 1474 637
Size: 837 3809
BciVI GTATCCnnnnn_n'
Cuts at: 2544 3305 4646 2544
Size: 761 1341 2544
Fragments arranged by size:
2544 1341 761
BclI T'GATC_A
Cuts at: 2166 2166
Size: 4646
BglII A'GATC_T
Cuts at: 367 2161 4198 367
Size: 1794 2037 815
Fragments arranged by size:
2037 1794 815
BlpI GC'TnA_GC
Cuts at: 1758 1758
Size: 4646
Bme1580I G_kGCm'C
Cuts at: 2271 2364 3420 2271
Size: 93 1056 3497
Fragments arranged by size:
3497 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 999 1305 1858 1939 2274 4418 999
Size: 306 553 81 335 2144 1227
Fragments arranged by size:
2144 1227 553 335 306 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3013 3013
Size: 4646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 785 1094 1535 682
Size: 103 309 441 3793
Fragments arranged by size:
3793 441 309 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2701 3193 3491 1822
Size: 879 492 298 2977
Fragments arranged by size:
2977 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 282 4350 282
Size: 4068 578
BsaAI yAC'GTr
Cuts at: 675 1999 675
Size: 1324 3322
BsaBI GATnn'nnATC
Cuts at: 6 2160 4197 6
Size: 2154 2037 455
Fragments arranged by size:
2154 2037 455
BsaHI Gr'CG_yC
Cuts at: 1317 2077 2325 3027 4523 1317
Size: 760 248 702 1496 1440
Fragments arranged by size:
1496 1440 760 702 248
BsaWI w'CCGG_w
Cuts at: 1835 2356 3034 3308 3455 3866 1835
Size: 521 678 274 147 411 2615
Fragments arranged by size:
2615 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1091 1091
Size: 4646
BseYI C'CCAG_C
Cuts at: 146 2071 2829 3406 3902 146
Size: 1925 758 577 496 890
Fragments arranged by size:
1925 890 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 497
Size: 4646
BsiEI CG_ry'CG
Cuts at: 104 2188 2234 3442 104
Size: 2084 46 1208 1308
Fragments arranged by size:
2084 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1660 2438 2628 3420 1660
Size: 778 190 792 2886
Fragments arranged by size:
2886 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 153 1660 2271 2364 2438 2628 2690 3420 153
Size: 1507 611 93 74 190 62 730 1379
Fragments arranged by size:
1507 1379 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3866 3866
Size: 4646
BspHI T'CATG_A
Cuts at: 3822 3822
Size: 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1696 1910 2212 2593 1696
Size: 214 302 381 3749
Fragments arranged by size:
3749 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2938 2992 4476 4641 50
Size: 2888 54 1484 165 55
Fragments arranged by size:
2888 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1296 1353 2558 1296
Size: 57 1205 3384
Fragments arranged by size:
3384 1205 57
BsrFI r'CCGG_y
Cuts at: 2644 2825 3034 2644
Size: 181 209 4256
Fragments arranged by size:
4256 209 181
BsrGI T'GTAC_A
Cuts at: 1112 1112
Size: 4646
BssSI C'ACGA_G
Cuts at: 1160 2917 3275 1160
Size: 1757 358 2531
Fragments arranged by size:
2531 1757 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1624 2023 1624
Size: 399 4247
BstBI TT'CG_AA
Cuts at: 3007 3007
Size: 4646
BstYI r'GATC_y
Cuts at: 1 367 1605 1671 2138 2161 2496 2742 3754
Size: 366 1238 66 467 23 335 246 1012
Cuts at: 3754 3840 3852 4198 1
Size: 86 12 346 449
Fragments arranged by size:
1238 1012 467 449 366 346 335 246
86 66 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 225 2596 2777 2940 225
Size: 2371 181 163 1931
Fragments arranged by size:
2371 1931 181 163
BtsI GCAGTG_nn'
Cuts at: 891 1889 891
Size: 998 3648
ClaI AT'CG_AT
Cuts at: 1311 1311
Size: 4646
DraI TTT'AAA
Cuts at: 1645 1645
Size: 4646
DrdI GACnn_nn'nnGTC
Cuts at: 2352 3210 2352
Size: 858 3788
EaeI y'GGCC_r
Cuts at: 903 1183 1359 2231 2405 2796 2823 4168 903
Size: 280 176 872 174 391 27 1345 1381
Fragments arranged by size:
1381 1345 872 391 280 176 174 27
EagI C'GGCC_G
Cuts at: 2231 2231
Size: 4646
EarI CTCTTCn'nnn_
Cuts at: 2669 2879 2669
Size: 210 4436
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2854 3164 3310 4456 2854
Size: 310 146 1146 3044
Fragments arranged by size:
3044 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1658 1658
Size: 4646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 785 1094 1535 1622 2471 2903 3650 3918
Size: 103 309 441 87 849 432 747 268
Cuts at: 3918 682
Size: 1410
Fragments arranged by size:
1410 849 747 441 432 309 268 103
87
EcoO109I rG'GnC_Cy
Cuts at: 1785 1785
Size: 4646
EcoRI G'AATT_C
Cuts at: 916 1650 916
Size: 734 3912
EcoRV GAT'ATC
Cuts at: 594 594
Size: 4646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 851 2665 851
Size: 1814 2832
FspI TGC'GCA
Cuts at: 2427 2427
Size: 4646
HaeII r_GCGC'y
Cuts at: 1030 1320 2080 2328 3350 4329 1030
Size: 290 760 248 1022 979 1347
Fragments arranged by size:
1347 1022 979 760 290 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 958 990 1016 1048 1438 1470 2488 2518 2520
Size: 32 26 32 390 32 1018 30 2
Cuts at: 2520 2550 958
Size: 30 3054
Fragments arranged by size:
3054 1018 390 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 1685 1685
Size: 4646
KasI G'GCGC_C
Cuts at: 1316 2076 2324 1316
Size: 760 248 3638
Fragments arranged by size:
3638 760 248
KpnI G_GTAC'C
Cuts at: 1666 1666
Size: 4646
MfeI C'AATT_G
Cuts at: 1533 1533
Size: 4646
MluI A'CGCG_T
Cuts at: 4079 4079
Size: 4646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1107 3317 3501 1107
Size: 2210 184 2252
Fragments arranged by size:
2252 2210 184
MscI TGG'CCA
Cuts at: 1185 1361 2407 1185
Size: 176 1046 3424
Fragments arranged by size:
3424 1046 176
MslI CAynn'nnrTG
Cuts at: 458 2635 2762 458
Size: 2177 127 2342
Fragments arranged by size:
2342 2177 127
MspA1I CmG'CkG
Cuts at: 1757 2071 2431 3444 3689 1757
Size: 314 360 1013 245 2714
Fragments arranged by size:
2714 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2827 2827
Size: 4646
NarI GG'CG_CC
Cuts at: 1317 2077 2325 1317
Size: 760 248 3638
Fragments arranged by size:
3638 760 248
NdeI CA'TA_TG
Cuts at: 455 455
Size: 4646
NgoMIV G'CCGG_C
Cuts at: 2825 2825
Size: 4646
NspI r_CATG'y
Cuts at: 759 1699 2199 3106 759
Size: 940 500 907 2299
Fragments arranged by size:
2299 940 907 500
PciI A'CATG_T
Cuts at: 755 3102 755
Size: 2347 2299
PflMI CCAn_nnn'nTGG
Cuts at: 547 547
Size: 4646
PmeI GTTT'AAAC
Cuts at: 1645 1645
Size: 4646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4614
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 474 506 474
Size: 32 4614
PstI C_TGCA'G
Cuts at: 825 1693 2378 825
Size: 868 685 3093
Fragments arranged by size:
3093 868 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4646
PvuII CAG'CTG
Cuts at: 2071 2431 2071
Size: 360 4286
RsrII CG'GwC_CG
Cuts at: 2841 2841
Size: 4646
SacI G_AGCT'C
Cuts at: 1660 1660
Size: 4646
SalI G'TCGA_C
Cuts at: 1683 1683
Size: 4646
SapI GCTCTTCn'nnn_
Cuts at: 2669 2879 2669
Size: 210 4436
SbfI CC_TGCA'GG
Cuts at: 825 1693 825
Size: 868 3778
ScaI AGT'ACT
Cuts at: 4163 4163
Size: 4646
SfcI C'TryA_G
Cuts at: 33 821 1689 2374 3367 3558 33
Size: 788 868 685 993 191 1121
Fragments arranged by size:
1121 993 868 788 685 191
SfoI GGC'GCC
Cuts at: 1318 2078 2326 1318
Size: 760 248 3638
Fragments arranged by size:
3638 760 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
SmaI CCC'GGG
Cuts at: 1668 1668
Size: 4646
SmlI C'TyrA_G
Cuts at: 1801 2716 3208 3470 1801
Size: 915 492 262 2977
Fragments arranged by size:
2977 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4646
SphI G_CATG'C
Cuts at: 1699 2199 1699
Size: 500 4146
StyI C'CwwG_G
Cuts at: 322 1780 2134 322
Size: 1458 354 2834
Fragments arranged by size:
2834 1458 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 853 2229 2362 2821 3030 853
Size: 1376 133 459 209 2469
Fragments arranged by size:
2469 1376 459 209 133
TatI w'GTAC_w
Cuts at: 1112 4161 1112
Size: 3049 1597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4163 4195 4163
Size: 32 4614
Tth111I GACn'n_nGTC
Cuts at: 1297 2443 1297
Size: 1146 3500
XbaI T'CTAG_A
Cuts at: 1677 1677
Size: 4646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 548 1405 2141 548
Size: 857 736 3053
Fragments arranged by size:
3053 857 736
XmaI C'CCGG_G
Cuts at: 1666 1666
Size: 4646
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NcoI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI