Restriction map for
pF1KE5136
(Circular) (Six-base) MAPSORT of: pF1KE5136.seq Check: 9585 from: 1 to: 3521
pF1KE5136 3521 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 139 559 139
Size: 420 3101
Acc65I G'GTAC_C
Cuts at: 537 537
Size: 3521
AclI AA'CG_TT
Cuts at: 3361 3361
Size: 3521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1346 1778 2525 2793 1346
Size: 432 747 268 2074
Fragments arranged by size:
2074 747 432 268
AfeI AGC'GCT
Cuts at: 195 195
Size: 3521
AflIII A'CryG_T
Cuts at: 873 1977 2954 873
Size: 1104 977 1440
Fragments arranged by size:
1440 1104 977
AgeI A'CCGG_T
Cuts at: 1909 1909
Size: 3521
AlwNI CAG_nnn'CTG
Cuts at: 318 826 2393 318
Size: 508 1567 1446
Fragments arranged by size:
1567 1446 508
ApaLI G'TGCA_C
Cuts at: 2291 2291
Size: 3521
ApoI r'AATT_y
Cuts at: 525 2874 3130 525
Size: 2349 256 916
Fragments arranged by size:
2349 916 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3521
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3521
AvaI C'yCGr_G
Cuts at: 541 541
Size: 3521
BamHI G'GATC_C
Cuts at: 546 546
Size: 3521
BanI G'GyrC_C
Cuts at: 153 241 537 951 1199 1234 153
Size: 88 296 414 248 35 2440
Fragments arranged by size:
2440 414 296 248 88 35
BanII G_rGCy'C
Cuts at: 370 535 1565 370
Size: 165 1030 2326
Fragments arranged by size:
2326 1030 165
BbeI G_GCGC'C
Cuts at: 157 955 1203 157
Size: 798 248 2475
Fragments arranged by size:
2475 798 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 112 146 112
Size: 34 3487
BciVI GTATCCnnnnn_n'
Cuts at: 1419 2180 3521 1419
Size: 761 1341 1419
Fragments arranged by size:
1419 1341 761
BclI T'GATC_A
Cuts at: 1041 1041
Size: 3521
BglI GCCn_nnn'nGGC
Cuts at: 178 178
Size: 3521
BglII A'GATC_T
Cuts at: 1036 3073 1036
Size: 2037 1484
BlpI GC'TnA_GC
Cuts at: 633 633
Size: 3521
Bme1580I G_kGCm'C
Cuts at: 244 1146 1239 2295 244
Size: 902 93 1056 1470
Fragments arranged by size:
1470 1056 902 93
BmrI ACTGGGnnnn_n'
Cuts at: 733 814 1149 3293 733
Size: 81 335 2144 961
Fragments arranged by size:
2144 961 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1576 2068 2366 697
Size: 879 492 298 1852
Fragments arranged by size:
1852 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3225 3225
Size: 3521
BsaAI yAC'GTr
Cuts at: 874 874
Size: 3521
BsaBI GATnn'nnATC
Cuts at: 6 1035 3072 6
Size: 1029 2037 455
Fragments arranged by size:
2037 1029 455
BsaHI Gr'CG_yC
Cuts at: 154 952 1200 1902 3398 154
Size: 798 248 702 1496 277
Fragments arranged by size:
1496 798 702 277 248
BsaWI w'CCGG_w
Cuts at: 710 1231 1909 2183 2330 2741 710
Size: 521 678 274 147 411 1490
Fragments arranged by size:
1490 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 946 1704 2281 2777 946
Size: 758 577 496 1690
Fragments arranged by size:
1690 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 238 1063 1109 2317 104
Size: 134 825 46 1208 1308
Fragments arranged by size:
1308 1208 825 134 46
BsiHKAI G_wGCw'C
Cuts at: 370 535 1313 1503 2295 370
Size: 165 778 190 792 1596
Fragments arranged by size:
1596 792 778 190 165
Bsp1286I G_dGCh'C
Cuts at: 244 370 535 1146 1239 1313 1503 1565 2295
Size: 126 165 611 93 74 190 62 730
Cuts at: 2295 244
Size: 1470
Fragments arranged by size:
1470 730 611 190 165 126 93 74
62
BspEI T'CCGG_A
Cuts at: 2741 2741
Size: 3521
BspHI T'CATG_A
Cuts at: 2697 2697
Size: 3521
BspMI ACCTGCnnnn'nnnn_
Cuts at: 362 571 785 1087 1468 362
Size: 209 214 302 381 2415
Fragments arranged by size:
2415 381 302 214 209
BsrBI CCG'CTC
Cuts at: 50 1813 1867 3351 3516 50
Size: 1763 54 1484 165 55
Fragments arranged by size:
1763 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1433 1433
Size: 3521
BsrFI r'CCGG_y
Cuts at: 205 233 1519 1700 1909 205
Size: 28 1286 181 209 1817
Fragments arranged by size:
1817 1286 209 181 28
BssSI C'ACGA_G
Cuts at: 1792 2150 1792
Size: 358 3163
BstAPI GCAn_nnn'nTGC
Cuts at: 898 898
Size: 3521
BstBI TT'CG_AA
Cuts at: 1882 1882
Size: 3521
BstYI r'GATC_y
Cuts at: 1 260 546 1013 1036 1371 1617 2629 2715
Size: 259 286 467 23 335 246 1012 86
Cuts at: 2715 2727 3073 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 286 259 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 245 501 107
Size: 138 256 3127
Fragments arranged by size:
3127 256 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1471 1652 1815 1471
Size: 181 163 3177
Fragments arranged by size:
3177 181 163
BtsI GCAGTG_nn'
Cuts at: 764 764
Size: 3521
DraI TTT'AAA
Cuts at: 345 520 345
Size: 175 3346
DrdI GACnn_nn'nnGTC
Cuts at: 1227 2085 1227
Size: 858 2663
EaeI y'GGCC_r
Cuts at: 170 200 235 1106 1280 1671 1698 3043 170
Size: 30 35 871 174 391 27 1345 648
Fragments arranged by size:
1345 871 648 391 174 35 30 27
EagI C'GGCC_G
Cuts at: 235 1106 235
Size: 871 2650
EarI CTCTTCn'nnn_
Cuts at: 1544 1754 1544
Size: 210 3311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 253 1729 2039 2185 3331 253
Size: 1476 310 146 1146 443
Fragments arranged by size:
1476 1146 443 310 146
EcoICRI GAG'CTC
Cuts at: 368 533 368
Size: 165 3356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1346 1778 2525 2793 1346
Size: 432 747 268 2074
Fragments arranged by size:
2074 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 660 660
Size: 3521
EcoRI G'AATT_C
Cuts at: 525 525
Size: 3521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1540 1540
Size: 3521
FspI TGC'GCA
Cuts at: 335 1302 335
Size: 967 2554
HaeII r_GCGC'y
Cuts at: 157 197 318 955 1203 2225 3204 157
Size: 40 121 637 248 1022 979 474
Fragments arranged by size:
1022 979 637 474 248 121 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1363 1393 1395 1425 1363
Size: 30 2 30 3459
Fragments arranged by size:
3459 30 30 2
HincII GTy'rAC
Cuts at: 560 560
Size: 3521
KasI G'GCGC_C
Cuts at: 153 951 1199 153
Size: 798 248 2475
Fragments arranged by size:
2475 798 248
KpnI G_GTAC'C
Cuts at: 541 541
Size: 3521
MluI A'CGCG_T
Cuts at: 2954 2954
Size: 3521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2376 2192
Size: 184 3337
MscI TGG'CCA
Cuts at: 172 1282 172
Size: 1110 2411
MslI CAynn'nnrTG
Cuts at: 466 1510 1637 466
Size: 1044 127 2350
Fragments arranged by size:
2350 1044 127
MspA1I CmG'CkG
Cuts at: 373 503 632 946 1306 2319 2564 373
Size: 130 129 314 360 1013 245 1330
Fragments arranged by size:
1330 1013 360 314 245 130 129
NaeI GCC'GGC
Cuts at: 1702 1702
Size: 3521
NarI GG'CG_CC
Cuts at: 154 952 1200 154
Size: 798 248 2475
Fragments arranged by size:
2475 798 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3521
NgoMIV G'CCGG_C
Cuts at: 1700 1700
Size: 3521
NspI r_CATG'y
Cuts at: 574 1074 1981 574
Size: 500 907 2114
Fragments arranged by size:
2114 907 500
PciI A'CATG_T
Cuts at: 1977 1977
Size: 3521
PflMI CCAn_nnn'nTGG
Cuts at: 318 318
Size: 3521
PmeI GTTT'AAAC
Cuts at: 520 520
Size: 3521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 3489
PstI C_TGCA'G
Cuts at: 389 568 1253 389
Size: 179 685 2657
Fragments arranged by size:
2657 685 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3521
PvuII CAG'CTG
Cuts at: 946 1306 946
Size: 360 3161
RsrII CG'GwC_CG
Cuts at: 1716 1716
Size: 3521
SacI G_AGCT'C
Cuts at: 370 535 370
Size: 165 3356
SacII CC_GC'GG
Cuts at: 504 504
Size: 3521
SalI G'TCGA_C
Cuts at: 558 558
Size: 3521
SapI GCTCTTCn'nnn_
Cuts at: 1544 1754 1544
Size: 210 3311
SbfI CC_TGCA'GG
Cuts at: 568 568
Size: 3521
ScaI AGT'ACT
Cuts at: 3038 3038
Size: 3521
SfcI C'TryA_G
Cuts at: 33 385 564 1249 2242 2433 33
Size: 352 179 685 993 191 1121
Fragments arranged by size:
1121 993 685 352 191 179
SfoI GGC'GCC
Cuts at: 155 953 1201 155
Size: 798 248 2475
Fragments arranged by size:
2475 798 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3521
SgrAI Cr'CCGG_yG
Cuts at: 205 205
Size: 3521
SmaI CCC'GGG
Cuts at: 543 543
Size: 3521
SmlI C'TyrA_G
Cuts at: 676 1591 2083 2345 676
Size: 915 492 262 1852
Fragments arranged by size:
1852 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3521
SphI G_CATG'C
Cuts at: 574 1074 574
Size: 500 3021
StuI AGG'CCT
Cuts at: 403 403
Size: 3521
StyI C'CwwG_G
Cuts at: 107 473 655 1009 107
Size: 366 182 354 2619
Fragments arranged by size:
2619 366 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1104 1237 1696 1905 1104
Size: 133 459 209 2720
Fragments arranged by size:
2720 459 209 133
TatI w'GTAC_w
Cuts at: 3036 3036
Size: 3521
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3489
Tth111I GACn'n_nGTC
Cuts at: 1318 1318
Size: 3521
XbaI T'CTAG_A
Cuts at: 552 552
Size: 3521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1016 1016
Size: 3521
XmaI C'CCGG_G
Cuts at: 541 541
Size: 3521
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI