Restriction map for
pF1KE4516
(Circular) (Six-base) MAPSORT of: pF1KE4516.seq Check: 5844 from: 1 to: 4763
pF1KE4516 4763 bp
With 182 enzymes: SgfI *
December 8, 2011 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 123 863 123
Size: 740 4023
AccI GT'mk_AC
Cuts at: 794 1438 1801 794
Size: 644 363 3756
Fragments arranged by size:
3756 644 363
Acc65I G'GTAC_C
Cuts at: 1779 1779
Size: 4763
AclI AA'CG_TT
Cuts at: 4603 4603
Size: 4763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2588 3020 3767 4035 2588
Size: 432 747 268 3316
Fragments arranged by size:
3316 747 432 268
AfeI AGC'GCT
Cuts at: 1602 1602
Size: 4763
AflIII A'CryG_T
Cuts at: 2115 3219 4196 2115
Size: 1104 977 2682
Fragments arranged by size:
2682 1104 977
AgeI A'CCGG_T
Cuts at: 3151 3151
Size: 4763
AleI CACnn'nnGTG
Cuts at: 175 175
Size: 4763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1344 1344
Size: 4763
AlwNI CAG_nnn'CTG
Cuts at: 864 1641 1662 2068 3635 864
Size: 777 21 406 1567 1992
Fragments arranged by size:
1992 1567 777 406 21
ApaI G_GGCC'C
Cuts at: 504 969 504
Size: 465 4298
ApaLI G'TGCA_C
Cuts at: 3533 3533
Size: 4763
ApoI r'AATT_y
Cuts at: 1767 4116 4372 1767
Size: 2349 256 2158
Fragments arranged by size:
2349 2158 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4763
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
AvaI C'yCGr_G
Cuts at: 311 690 1502 1783 311
Size: 379 812 281 3291
Fragments arranged by size:
3291 812 379 281
BamHI G'GATC_C
Cuts at: 1788 1788
Size: 4763
BanI G'GyrC_C
Cuts at: 775 848 1779 2193 2441 2476 775
Size: 73 931 414 248 35 3062
Fragments arranged by size:
3062 931 414 248 73 35
BanII G_rGCy'C
Cuts at: 419 504 969 1525 1550 1777 2807 419
Size: 85 465 556 25 227 1030 2375
Fragments arranged by size:
2375 1030 556 465 227 85 25
BbeI G_GCGC'C
Cuts at: 852 2197 2445 852
Size: 1345 248 3170
Fragments arranged by size:
3170 1345 248
BbvCI CC'TCA_GC
Cuts at: 277 1734 277
Size: 1457 3306
BciVI GTATCCnnnnn_n'
Cuts at: 1463 2661 3422 4763 1463
Size: 1198 761 1341 1463
Fragments arranged by size:
1463 1341 1198 761
BclI T'GATC_A
Cuts at: 2283 2283
Size: 4763
BglI GCCn_nnn'nGGC
Cuts at: 846 1303 846
Size: 457 4306
BglII A'GATC_T
Cuts at: 2278 4315 2278
Size: 2037 2726
BlpI GC'TnA_GC
Cuts at: 1875 1875
Size: 4763
Bme1580I G_kGCm'C
Cuts at: 504 778 842 969 2388 2481 3537 504
Size: 274 64 127 1419 93 1056 1730
Fragments arranged by size:
1730 1419 1056 274 127 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 513 1451 1975 2056 2391 4535 513
Size: 938 524 81 335 2144 741
Fragments arranged by size:
2144 938 741 524 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 4763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1684 316
Size: 1368 3395
Bpu10I CC'TnA_GC
Cuts at: 277 1734 277
Size: 1457 3306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1939 2818 3310 3608 1939
Size: 879 492 298 3094
Fragments arranged by size:
3094 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 873 1618 4467 873
Size: 745 2849 1169
Fragments arranged by size:
2849 1169 745
BsaAI yAC'GTr
Cuts at: 2116 2116
Size: 4763
BsaBI GATnn'nnATC
Cuts at: 6 2277 4314 6
Size: 2271 2037 455
Fragments arranged by size:
2271 2037 455
BsaHI Gr'CG_yC
Cuts at: 849 2194 2442 3144 4640 849
Size: 1345 248 702 1496 972
Fragments arranged by size:
1496 1345 972 702 248
BsaWI w'CCGG_w
Cuts at: 1952 2473 3151 3425 3572 3983 1952
Size: 521 678 274 147 411 2732
Fragments arranged by size:
2732 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 542 572 922 952 542
Size: 30 350 30 4353
Fragments arranged by size:
4353 350 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 676 924 927 676
Size: 248 3 4512
Fragments arranged by size:
4512 248 3
BseYI C'CCAG_C
Cuts at: 1392 2188 2946 3523 4019 1392
Size: 796 758 577 496 2136
Fragments arranged by size:
2136 796 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 1292 1675 405
Size: 887 383 3493
Fragments arranged by size:
3493 887 383
BsiEI CG_ry'CG
Cuts at: 104 150 2305 2351 3559 104
Size: 46 2155 46 1208 1308
Fragments arranged by size:
2155 1308 1208 46 46
BsiHKAI G_wGCw'C
Cuts at: 493 1550 1706 1777 2555 2745 3537 493
Size: 1057 156 71 778 190 792 1719
Fragments arranged by size:
1719 1057 792 778 190 156 71
Bsp1286I G_dGCh'C
Cuts at: 419 493 504 778 842 969 1525 1550 1706
Size: 74 11 274 64 127 556 25 156
Cuts at: 1706 1777 2388 2481 2555 2745 2807 3537 419
Size: 71 611 93 74 190 62 730 1645
Fragments arranged by size:
1645 730 611 556 274 190 156 127
93 74 74 71 64 62 25 11
BspEI T'CCGG_A
Cuts at: 3983 3983
Size: 4763
BspHI T'CATG_A
Cuts at: 3939 3939
Size: 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 123 623 646 863 1193 1250 1813 2027 2329
Size: 500 23 217 330 57 563 214 302
Cuts at: 2329 2710 123
Size: 381 2176
Fragments arranged by size:
2176 563 500 381 330 302 217 214
57 23
BsrBI CCG'CTC
Cuts at: 50 3055 3109 4593 4758 50
Size: 3005 54 1484 165 55
Fragments arranged by size:
3005 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2675 2675
Size: 4763
BsrFI r'CCGG_y
Cuts at: 1289 2761 2942 3151 1289
Size: 1472 181 209 2901
Fragments arranged by size:
2901 1472 209 181
BsrGI T'GTAC_A
Cuts at: 527 527
Size: 4763
BssSI C'ACGA_G
Cuts at: 3034 3392 3034
Size: 358 4405
BstAPI GCAn_nnn'nTGC
Cuts at: 2140 2140
Size: 4763
BstBI TT'CG_AA
Cuts at: 3124 3124
Size: 4763
BstEII G'GTnAC_C
Cuts at: 168 168
Size: 4763
BstXI CCAn_nnnn'nTGG
Cuts at: 1257 1257
Size: 4763
BstYI r'GATC_y
Cuts at: 1 161 434 1788 2255 2278 2613 2859 3871
Size: 160 273 1354 467 23 335 246 1012
Cuts at: 3871 3957 3969 4315 1
Size: 86 12 346 449
Fragments arranged by size:
1354 1012 467 449 346 335 273 246
160 86 23 12
BstZ17I GTA'TAC
Cuts at: 1439 1439
Size: 4763
Bsu36I CC'TnA_GG
Cuts at: 961 961
Size: 4763
BtgI C'CryG_G
Cuts at: 107 554 1061 1085 1496 107
Size: 447 507 24 411 3374
Fragments arranged by size:
3374 507 447 411 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2713 2894 3057 2713
Size: 181 163 4419
Fragments arranged by size:
4419 181 163
BtsI GCAGTG_nn'
Cuts at: 227 249 367 1309 1639 1723 2006 227
Size: 22 118 942 330 84 283 2984
Fragments arranged by size:
2984 942 330 283 118 84 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1140 1175 1140
Size: 35 4728
DraI TTT'AAA
Cuts at: 1762 1762
Size: 4763
DrdI GACnn_nn'nnGTC
Cuts at: 627 2469 3327 627
Size: 1842 858 2063
Fragments arranged by size:
2063 1842 858
EaeI y'GGCC_r
Cuts at: 147 1082 1592 2348 2522 2913 2940 4285 147
Size: 935 510 756 174 391 27 1345 625
Fragments arranged by size:
1345 935 756 625 510 391 174 27
EagI C'GGCC_G
Cuts at: 147 2348 147
Size: 2201 2562
EarI CTCTTCn'nnn_
Cuts at: 1126 1573 2786 2996 1126
Size: 447 1213 210 2893
Fragments arranged by size:
2893 1213 447 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1102 2971 3281 3427 4573 1102
Size: 1869 310 146 1146 1292
Fragments arranged by size:
1869 1292 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1548 1775 1548
Size: 227 4536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1684 2588 3020 3767 4035 316
Size: 1368 904 432 747 268 1044
Fragments arranged by size:
1368 1044 904 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 130 942 1216 130
Size: 812 274 3677
Fragments arranged by size:
3677 812 274
EcoO109I rG'GnC_Cy
Cuts at: 480 500 501 635 965 966 1659 1902 480
Size: 20 1 134 330 1 693 243 3341
Fragments arranged by size:
3341 693 330 243 134 20 1 1
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4763
FspI TGC'GCA
Cuts at: 642 2544 642
Size: 1902 2861
HaeII r_GCGC'y
Cuts at: 852 1604 2197 2445 3467 4446 852
Size: 752 593 248 1022 979 1169
Fragments arranged by size:
1169 1022 979 752 593 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 1190 1222 2605 2635 2637 2667 189
Size: 32 969 32 1383 30 2 30 2285
Fragments arranged by size:
2285 1383 969 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1802 1802
Size: 4763
HindIII A'AGCT_T
Cuts at: 1252 1252
Size: 4763
KasI G'GCGC_C
Cuts at: 848 2193 2441 848
Size: 1345 248 3170
Fragments arranged by size:
3170 1345 248
KpnI G_GTAC'C
Cuts at: 1783 1783
Size: 4763
MluI A'CGCG_T
Cuts at: 4196 4196
Size: 4763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3434 3618 3434
Size: 184 4579
MscI TGG'CCA
Cuts at: 2524 2524
Size: 4763
MslI CAynn'nnrTG
Cuts at: 175 1483 2752 2879 175
Size: 1308 1269 127 2059
Fragments arranged by size:
2059 1308 1269 127
MspA1I CmG'CkG
Cuts at: 513 625 926 1874 2188 2548 3561 3806 513
Size: 112 301 948 314 360 1013 245 1470
Fragments arranged by size:
1470 1013 948 360 314 301 245 112
NaeI GCC'GGC
Cuts at: 1291 2944 1291
Size: 1653 3110
NarI GG'CG_CC
Cuts at: 849 2194 2442 849
Size: 1345 248 3170
Fragments arranged by size:
3170 1345 248
NcoI C'CATG_G
Cuts at: 107 554 1061 1085 1496 107
Size: 447 507 24 411 3374
Fragments arranged by size:
3374 507 447 411 24
NgoMIV G'CCGG_C
Cuts at: 1289 2942 1289
Size: 1653 3110
NspI r_CATG'y
Cuts at: 1816 2316 3223 1816
Size: 500 907 3356
Fragments arranged by size:
3356 907 500
PasI CC'CwG_GG
Cuts at: 1281 1629 1281
Size: 348 4415
PciI A'CATG_T
Cuts at: 3219 3219
Size: 4763
PflMI CCAn_nnn'nTGG
Cuts at: 1142 1142
Size: 4763
PfoI T'CCnGG_A
Cuts at: 940 940
Size: 4763
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4731
PpuMI rG'GwC_Cy
Cuts at: 480 635 1659 480
Size: 155 1024 3584
Fragments arranged by size:
3584 1024 155
PspOMI G'GGCC_C
Cuts at: 500 965 500
Size: 465 4298
PstI C_TGCA'G
Cuts at: 131 221 620 860 974 1049 1190 1730 1810
Size: 90 399 240 114 75 141 540 80
Cuts at: 1810 2495 131
Size: 685 2399
Fragments arranged by size:
2399 685 540 399 240 141 114 90
80 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4763
PvuII CAG'CTG
Cuts at: 513 625 2188 2548 513
Size: 112 1563 360 2728
Fragments arranged by size:
2728 1563 360 112
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4763
SacI G_AGCT'C
Cuts at: 1550 1777 1550
Size: 227 4536
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4763
SapI GCTCTTCn'nnn_
Cuts at: 2786 2996 2786
Size: 210 4553
SbfI CC_TGCA'GG
Cuts at: 1190 1810 1190
Size: 620 4143
ScaI AGT'ACT
Cuts at: 4280 4280
Size: 4763
SfcI C'TryA_G
Cuts at: 33 127 217 616 795 856 970 1045 1186
Size: 94 90 399 179 61 114 75 141
Cuts at: 1186 1726 1806 2491 3484 3675 33
Size: 540 80 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 399 191 179 141
114 94 90 80 75 61
SfoI GGC'GCC
Cuts at: 850 2195 2443 850
Size: 1345 248 3170
Fragments arranged by size:
3170 1345 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
SmaI CCC'GGG
Cuts at: 313 1785 313
Size: 1472 3291
SmlI C'TyrA_G
Cuts at: 1918 2833 3325 3587 1918
Size: 915 492 262 3094
Fragments arranged by size:
3094 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4763
SphI G_CATG'C
Cuts at: 1816 2316 1816
Size: 500 4263
StuI AGG'CCT
Cuts at: 1732 1732
Size: 4763
StyI C'CwwG_G
Cuts at: 107 554 1061 1085 1496 1897 2251 107
Size: 447 507 24 411 401 354 2619
Fragments arranged by size:
2619 507 447 411 401 354 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 669 2346 2479 2938 3147 669
Size: 1677 133 459 209 2285
Fragments arranged by size:
2285 1677 459 209 133
TatI w'GTAC_w
Cuts at: 527 4278 527
Size: 3751 1012
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 472 504 542 574 644 676 922 954 4280
Size: 32 38 32 70 32 246 32 3326
Cuts at: 4280 4312 472
Size: 32 923
Fragments arranged by size:
3326 923 246 70 38 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2560 2560
Size: 4763
XbaI T'CTAG_A
Cuts at: 1794 1794
Size: 4763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1020 1225 1503 2258 1020
Size: 205 278 755 3525
Fragments arranged by size:
3525 755 278 205
XmaI C'CCGG_G
Cuts at: 311 1783 311
Size: 1472 3291
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI