Restriction map for
pF1KE4450
(Circular) (Six-base) MAPSORT of: pF1KE4450.seq Check: 3064 from: 1 to: 4517
pF1KE4450 4517 bp
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 912 912
Size: 4517
AccI GT'mk_AC
Cuts at: 527 1555 527
Size: 1028 3489
Acc65I G'GTAC_C
Cuts at: 1533 1533
Size: 4517
AclI AA'CG_TT
Cuts at: 4357 4357
Size: 4517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 2342 2774 3521 3789 824
Size: 1518 432 747 268 1552
Fragments arranged by size:
1552 1518 747 432 268
AflIII A'CryG_T
Cuts at: 1869 2973 3950 1869
Size: 1104 977 2436
Fragments arranged by size:
2436 1104 977
AgeI A'CCGG_T
Cuts at: 2905 2905
Size: 4517
AlwNI CAG_nnn'CTG
Cuts at: 1024 1822 3389 1024
Size: 798 1567 2152
Fragments arranged by size:
2152 1567 798
ApaI G_GGCC'C
Cuts at: 386 386
Size: 4517
ApaLI G'TGCA_C
Cuts at: 3287 3287
Size: 4517
ApoI r'AATT_y
Cuts at: 1521 3870 4126 1521
Size: 2349 256 1912
Fragments arranged by size:
2349 1912 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4517
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
AvaI C'yCGr_G
Cuts at: 1537 1537
Size: 4517
BamHI G'GATC_C
Cuts at: 991 1542 991
Size: 551 3966
BanI G'GyrC_C
Cuts at: 113 1533 1947 2195 2230 113
Size: 1420 414 248 35 2400
Fragments arranged by size:
2400 1420 414 248 35
BanII G_rGCy'C
Cuts at: 386 959 1531 2561 386
Size: 573 572 1030 2342
Fragments arranged by size:
2342 1030 573 572
BbeI G_GCGC'C
Cuts at: 1951 2199 1951
Size: 248 4269
BbsI GAAGACnn'nnnn_
Cuts at: 1293 1480 1293
Size: 187 4330
BbvCI CC'TCA_GC
Cuts at: 871 1249 871
Size: 378 4139
BciVI GTATCCnnnnn_n'
Cuts at: 2415 3176 4517 2415
Size: 761 1341 2415
Fragments arranged by size:
2415 1341 761
BclI T'GATC_A
Cuts at: 2037 2037
Size: 4517
BfrBI ATG'CAT
Cuts at: 421 421
Size: 4517
BglII A'GATC_T
Cuts at: 489 2032 4069 489
Size: 1543 2037 937
Fragments arranged by size:
2037 1543 937
BlpI GC'TnA_GC
Cuts at: 1629 1629
Size: 4517
Bme1580I G_kGCm'C
Cuts at: 116 386 2142 2235 3291 116
Size: 270 1756 93 1056 1342
Fragments arranged by size:
1756 1342 1056 270 93
BmrI ACTGGGnnnn_n'
Cuts at: 760 784 1729 1810 2145 4289 760
Size: 24 945 81 335 2144 988
Fragments arranged by size:
2144 988 945 335 81 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 4517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 540
Size: 4517
Bpu10I CC'TnA_GC
Cuts at: 871 1249 871
Size: 378 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2572 3064 3362 1693
Size: 879 492 298 2848
Fragments arranged by size:
2848 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4221 4221
Size: 4517
BsaAI yAC'GTr
Cuts at: 1870 1870
Size: 4517
BsaBI GATnn'nnATC
Cuts at: 6 2031 4068 6
Size: 2025 2037 455
Fragments arranged by size:
2037 2025 455
BsaHI Gr'CG_yC
Cuts at: 1948 2196 2898 4394 1948
Size: 248 702 1496 2071
Fragments arranged by size:
2071 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1706 2227 2905 3179 3326 3737 1706
Size: 521 678 274 147 411 2486
Fragments arranged by size:
2486 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 209 284 1279 1942 2700 3277 3773 209
Size: 75 995 663 758 577 496 953
Fragments arranged by size:
995 953 758 663 577 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 890
Size: 4517
BsiEI CG_ry'CG
Cuts at: 104 399 2059 2105 3313 104
Size: 295 1660 46 1208 1308
Fragments arranged by size:
1660 1308 1208 295 46
BsiHKAI G_wGCw'C
Cuts at: 959 1531 2309 2499 3291 959
Size: 572 778 190 792 2185
Fragments arranged by size:
2185 792 778 572 190
Bsp1286I G_dGCh'C
Cuts at: 116 386 959 1531 2142 2235 2309 2499 2561
Size: 270 573 572 611 93 74 190 62
Cuts at: 2561 3291 116
Size: 730 1342
Fragments arranged by size:
1342 730 611 573 572 270 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3737 3737
Size: 4517
BspHI T'CATG_A
Cuts at: 3693 3693
Size: 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 912 1009 1156 1567 1781 2083 2464 912
Size: 97 147 411 214 302 381 2965
Fragments arranged by size:
2965 411 381 302 214 147 97
BsrBI CCG'CTC
Cuts at: 50 2809 2863 4347 4512 50
Size: 2759 54 1484 165 55
Fragments arranged by size:
2759 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 2429 1394
Size: 1035 3482
BsrFI r'CCGG_y
Cuts at: 1477 2515 2696 2905 1477
Size: 1038 181 209 3089
Fragments arranged by size:
3089 1038 209 181
BssSI C'ACGA_G
Cuts at: 2788 3146 2788
Size: 358 4159
BstAPI GCAn_nnn'nTGC
Cuts at: 1894 1894
Size: 4517
BstBI TT'CG_AA
Cuts at: 2878 2878
Size: 4517
BstXI CCAn_nnnn'nTGG
Cuts at: 576 576
Size: 4517
BstYI r'GATC_y
Cuts at: 1 489 991 1542 2009 2032 2367 2613 3625
Size: 488 502 551 467 23 335 246 1012
Cuts at: 3625 3711 3723 4069 1
Size: 86 12 346 449
Fragments arranged by size:
1012 551 502 488 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 528 528
Size: 4517
BtgI C'CryG_G
Cuts at: 107 107
Size: 4517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2467 2648 2811 2467
Size: 181 163 4173
Fragments arranged by size:
4173 181 163
BtsI GCAGTG_nn'
Cuts at: 203 789 1760 203
Size: 586 971 2960
Fragments arranged by size:
2960 971 586
DraI TTT'AAA
Cuts at: 1516 1516
Size: 4517
DraIII CAC_nnn'GTG
Cuts at: 138 138
Size: 4517
DrdI GACnn_nn'nnGTC
Cuts at: 2223 3081 2223
Size: 858 3659
EaeI y'GGCC_r
Cuts at: 2102 2276 2667 2694 4039 2102
Size: 174 391 27 1345 2580
Fragments arranged by size:
2580 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2102 2102
Size: 4517
EarI CTCTTCn'nnn_
Cuts at: 2540 2750 2540
Size: 210 4307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2725 3035 3181 4327 2725
Size: 310 146 1146 2915
Fragments arranged by size:
2915 1146 310 146
EcoICRI GAG'CTC
Cuts at: 957 1529 957
Size: 572 3945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 824 2342 2774 3521 3789 540
Size: 284 1518 432 747 268 1268
Fragments arranged by size:
1518 1268 747 432 284 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1215 1215
Size: 4517
EcoO109I rG'GnC_Cy
Cuts at: 382 701 739 1656 382
Size: 319 38 917 3243
Fragments arranged by size:
3243 917 319 38
EcoRI G'AATT_C
Cuts at: 1521 1521
Size: 4517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4517
FspI TGC'GCA
Cuts at: 2298 2298
Size: 4517
HaeII r_GCGC'y
Cuts at: 1951 2199 3221 4200 1951
Size: 248 1022 979 2268
Fragments arranged by size:
2268 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 636 668 693 725 1117 1149 2359 2389 2391
Size: 32 25 32 392 32 1210 30 2
Cuts at: 2391 2421 636
Size: 30 2732
Fragments arranged by size:
2732 1210 392 32 32 32 30 30
25 2
HincII GTy'rAC
Cuts at: 1556 1556
Size: 4517
KasI G'GCGC_C
Cuts at: 1947 2195 1947
Size: 248 4269
KpnI G_GTAC'C
Cuts at: 1537 1537
Size: 4517
MluI A'CGCG_T
Cuts at: 3950 3950
Size: 4517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 593 3188 3372 593
Size: 2595 184 1738
Fragments arranged by size:
2595 1738 184
MscI TGG'CCA
Cuts at: 2278 2278
Size: 4517
MslI CAynn'nnrTG
Cuts at: 976 1492 2506 2633 976
Size: 516 1014 127 2860
Fragments arranged by size:
2860 1014 516 127
MspA1I CmG'CkG
Cuts at: 305 395 684 1628 1942 2302 3315 3560 305
Size: 90 289 944 314 360 1013 245 1262
Fragments arranged by size:
1262 1013 944 360 314 289 245 90
NaeI GCC'GGC
Cuts at: 2698 2698
Size: 4517
NarI GG'CG_CC
Cuts at: 1948 2196 1948
Size: 248 4269
NcoI C'CATG_G
Cuts at: 107 107
Size: 4517
NgoMIV G'CCGG_C
Cuts at: 2696 2696
Size: 4517
NsiI A_TGCA'T
Cuts at: 423 423
Size: 4517
NspI r_CATG'y
Cuts at: 1457 1570 2070 2977 1457
Size: 113 500 907 2997
Fragments arranged by size:
2997 907 500 113
PasI CC'CwG_GG
Cuts at: 379 1233 379
Size: 854 3663
PciI A'CATG_T
Cuts at: 2973 2973
Size: 4517
PflMI CCAn_nnn'nTGG
Cuts at: 1181 1181
Size: 4517
PmeI GTTT'AAAC
Cuts at: 1516 1516
Size: 4517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4485
PpuMI rG'GwC_Cy
Cuts at: 701 739 701
Size: 38 4479
PshAI GACnn'nnGTC
Cuts at: 1042 1114 1042
Size: 72 4445
PspOMI G'GGCC_C
Cuts at: 382 382
Size: 4517
PstI C_TGCA'G
Cuts at: 683 1564 2249 683
Size: 881 685 2951
Fragments arranged by size:
2951 881 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4517
PvuII CAG'CTG
Cuts at: 305 684 1942 2302 305
Size: 379 1258 360 2520
Fragments arranged by size:
2520 1258 379 360
RsrII CG'GwC_CG
Cuts at: 2712 2712
Size: 4517
SacI G_AGCT'C
Cuts at: 959 1531 959
Size: 572 3945
SalI G'TCGA_C
Cuts at: 1554 1554
Size: 4517
SapI GCTCTTCn'nnn_
Cuts at: 2540 2750 2540
Size: 210 4307
SbfI CC_TGCA'GG
Cuts at: 1564 1564
Size: 4517
ScaI AGT'ACT
Cuts at: 913 4034 913
Size: 3121 1396
SfcI C'TryA_G
Cuts at: 33 300 679 1560 2245 3238 3429 33
Size: 267 379 881 685 993 191 1121
Fragments arranged by size:
1121 993 881 685 379 267 191
SfoI GGC'GCC
Cuts at: 1949 2197 1949
Size: 248 4269
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
SmaI CCC'GGG
Cuts at: 1539 1539
Size: 4517
SmlI C'TyrA_G
Cuts at: 1672 2587 3079 3341 1672
Size: 915 492 262 2848
Fragments arranged by size:
2848 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4517
SphI G_CATG'C
Cuts at: 1570 2070 1570
Size: 500 4017
StyI C'CwwG_G
Cuts at: 107 1651 2005 107
Size: 1544 354 2619
Fragments arranged by size:
2619 1544 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 2100 2233 2692 2901 460
Size: 1640 133 459 209 2076
Fragments arranged by size:
2076 1640 459 209 133
TatI w'GTAC_w
Cuts at: 911 4032 911
Size: 3121 1396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4034 4066 4034
Size: 32 4485
Tth111I GACn'n_nGTC
Cuts at: 790 2314 790
Size: 1524 2993
XbaI T'CTAG_A
Cuts at: 1548 1548
Size: 4517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 292 2012 292
Size: 1720 2797
XmaI C'CCGG_G
Cuts at: 1537 1537
Size: 4517
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PfoI PmlI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI