Restriction map for
pF1KE4294
(Circular) (Six-base) MAPSORT of: pF1KE4294.seq Check: 460 from: 1 to: 9158
pF1KE4294 9158 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3989 4999 5834 3989
Size: 1010 835 7313
Fragments arranged by size:
7313 1010 835
AatII G_ACGT'C
Cuts at: 4304 4304
Size: 9158
AccI GT'mk_AC
Cuts at: 911 6196 911
Size: 5285 3873
Acc65I G'GTAC_C
Cuts at: 3890 6174 3890
Size: 2284 6874
AclI AA'CG_TT
Cuts at: 8998 8998
Size: 9158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 1483 1837 1934 4288 5799 6983 7415 8162
Size: 108 354 97 2354 1511 1184 432 747
Cuts at: 8162 8430 1375
Size: 268 2103
Fragments arranged by size:
2354 2103 1511 1184 747 432 354 268
108 97
AfeI AGC'GCT
Cuts at: 1699 3919 1699
Size: 2220 6938
AflIII A'CryG_T
Cuts at: 5462 6510 7614 8591 5462
Size: 1048 1104 977 6029
Fragments arranged by size:
6029 1104 1048 977
AgeI A'CCGG_T
Cuts at: 7546 7546
Size: 9158
AhdI GACnn_n'nnGTC
Cuts at: 2787 4269 2787
Size: 1482 7676
AleI CACnn'nnGTG
Cuts at: 4756 4798 6016 4756
Size: 42 1218 7898
Fragments arranged by size:
7898 1218 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 826 826
Size: 9158
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4564 4596 4564
Size: 32 9126
AlwNI CAG_nnn'CTG
Cuts at: 378 2619 2715 3030 3759 5592 5826 6463 8030
Size: 2241 96 315 729 1833 234 637 1567
Cuts at: 8030 378
Size: 1506
Fragments arranged by size:
2241 1833 1567 1506 729 637 315 234
96
ApaI G_GGCC'C
Cuts at: 2097 3627 3888 2097
Size: 1530 261 7367
Fragments arranged by size:
7367 1530 261
ApaLI G'TGCA_C
Cuts at: 6021 7928 6021
Size: 1907 7251
ApoI r'AATT_y
Cuts at: 1729 3934 5022 6162 8511 8767 1729
Size: 2205 1088 1140 2349 256 2120
Fragments arranged by size:
2349 2205 2120 1140 1088 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9158
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9158
AvaI C'yCGr_G
Cuts at: 317 4221 5957 6178 317
Size: 3904 1736 221 3297
Fragments arranged by size:
3904 3297 1736 221
AvrII C'CTAG_G
Cuts at: 4371 4371
Size: 9158
BamHI G'GATC_C
Cuts at: 6183 6183
Size: 9158
BanI G'GyrC_C
Cuts at: 205 313 599 2818 3128 3868 3890 3977 4366
Size: 108 286 2219 310 740 22 87 389
Cuts at: 4366 5086 5363 5526 5920 6050 6076 6174 6588
Size: 720 277 163 394 130 26 98 414
Cuts at: 6588 6836 6871 205
Size: 248 35 2492
Fragments arranged by size:
2492 2219 740 720 414 394 389 310
286 277 248 163 130 108 98 87
35 26 22
BanII G_rGCy'C
Cuts at: 461 956 1835 1868 2097 2147 2926 3377 3627
Size: 495 879 33 229 50 779 451 250
Cuts at: 3627 3888 4115 5294 6172 7202 461
Size: 261 227 1179 878 1030 2417
Fragments arranged by size:
2417 1179 1030 879 878 779 495 451
261 250 229 227 50 33
BbeI G_GCGC'C
Cuts at: 317 4370 5924 6080 6592 6840 317
Size: 4053 1554 156 512 248 2635
Fragments arranged by size:
4053 2635 1554 512 248 156
BbsI GAAGACnn'nnnn_
Cuts at: 734 1922 2969 3686 5165 734
Size: 1188 1047 717 1479 4727
Fragments arranged by size:
4727 1479 1188 1047 717
BbvCI CC'TCA_GC
Cuts at: 3973 3973
Size: 9158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 946 980 2586 2620 946
Size: 34 1606 34 7484
Fragments arranged by size:
7484 1606 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4965 7056 7817 9158 4965
Size: 2091 761 1341 4965
Fragments arranged by size:
4965 2091 1341 761
BclI T'GATC_A
Cuts at: 2030 2951 3215 3531 6678 2030
Size: 921 264 316 3147 4510
Fragments arranged by size:
4510 3147 921 316 264
BglI GCCn_nnn'nGGC
Cuts at: 484 2224 2671 3860 6001 484
Size: 1740 447 1189 2141 3641
Fragments arranged by size:
3641 2141 1740 1189 447
BglII A'GATC_T
Cuts at: 2075 5273 6673 8710 2075
Size: 3198 1400 2037 2523
Fragments arranged by size:
3198 2523 2037 1400
BlpI GC'TnA_GC
Cuts at: 1991 4123 6270 1991
Size: 2132 2147 4879
Fragments arranged by size:
4879 2147 2132
Bme1580I G_kGCm'C
Cuts at: 602 2097 3599 3627 3888 4387 4945 5089 5427
Size: 1495 1502 28 261 499 558 144 338
Cuts at: 5427 5584 6025 6783 6876 7932 602
Size: 157 441 758 93 1056 1828
Fragments arranged by size:
1828 1502 1495 1056 758 558 499 441
338 261 157 144 93 28
BmgBI CAC'GTC
Cuts at: 2571 2592 2706 3423 3516 5553 2571
Size: 21 114 717 93 2037 6176
Fragments arranged by size:
6176 2037 717 114 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 3635 4510 5176 6370 6451 6786 8930 3635
Size: 875 666 1194 81 335 2144 3863
Fragments arranged by size:
3863 2144 1194 875 666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5556 7525 5556
Size: 1969 7189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 427 1326 1605 2092 2131 2299 2454 2752
Size: 78 899 279 487 39 168 155 298
Cuts at: 2752 3826 3841 4129 4501 4855 4961 5290 5308
Size: 1074 15 288 372 354 106 329 18
Cuts at: 5308 5926 6084 6148 349
Size: 618 158 64 3359
Fragments arranged by size:
3359 1074 899 618 487 372 354 329
298 288 279 168 158 155 106 78
64 39 18 15
Bpu10I CC'TnA_GC
Cuts at: 1874 2650 3973 1874
Size: 776 1323 7059
Fragments arranged by size:
7059 1323 776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1560 1562 2148 4915 5661 6334 7213 7705
Size: 1013 2 586 2767 746 673 879 492
Cuts at: 7705 8003 547
Size: 298 1702
Fragments arranged by size:
2767 1702 1013 879 746 673 586 492
298 2
BsaI GGTCTCn'nnnn_
Cuts at: 296 5803 5849 8862 296
Size: 5507 46 3013 592
Fragments arranged by size:
5507 3013 592 46
BsaAI yAC'GTr
Cuts at: 2268 2576 3594 6511 2268
Size: 308 1018 2917 4915
Fragments arranged by size:
4915 2917 1018 308
BsaBI GATnn'nnATC
Cuts at: 6 2032 3214 5593 6672 8709 6
Size: 2026 1182 2379 1079 2037 455
Fragments arranged by size:
2379 2037 2026 1182 1079 455
BsaHI Gr'CG_yC
Cuts at: 314 2752 3574 4301 4367 5921 6077 6589 6837
Size: 2438 822 727 66 1554 156 512 248
Cuts at: 6837 7539 9035 314
Size: 702 1496 437
Fragments arranged by size:
2438 1554 1496 822 727 702 512 437
248 156 66
BsaWI w'CCGG_w
Cuts at: 1019 6347 6868 7546 7820 7967 8378 1019
Size: 5328 521 678 274 147 411 1799
Fragments arranged by size:
5328 1799 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1782 1812 3700 3730 5089 5119 5248 5278 5608
Size: 30 1888 30 1359 30 129 30 330
Cuts at: 5608 5638 1782
Size: 30 5302
Fragments arranged by size:
5302 1888 1359 330 129 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 346 2311 4792 5365 5587 5631 6127 175
Size: 171 1965 2481 573 222 44 496 3206
Fragments arranged by size:
3206 2481 1965 573 496 222 171 44
BseYI C'CCAG_C
Cuts at: 133 356 474 554 799 847 1878 1887 2289
Size: 223 118 80 245 48 1031 9 402
Cuts at: 2289 3626 4613 5446 5577 5686 5694 5924 6071
Size: 1337 987 833 131 109 8 230 147
Cuts at: 6071 6117 6583 7341 7918 8414 133
Size: 46 466 758 577 496 877
Fragments arranged by size:
1337 1031 987 877 833 758 577 496
466 402 245 230 223 147 131 118
109 80 48 46 9 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1087 2598 2728 3682 4024 4222 4481 4597
Size: 552 1511 130 954 342 198 259 116
Cuts at: 4597 4849 5349 535
Size: 252 500 4344
Fragments arranged by size:
4344 1511 954 552 500 342 259 252
198 130 116
BsiEI CG_ry'CG
Cuts at: 104 4639 6700 6746 7954 104
Size: 4535 2061 46 1208 1308
Fragments arranged by size:
4535 2061 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 956 1835 1868 1992 2147 3827 4115 4313 4469
Size: 879 33 124 155 1680 288 198 156
Cuts at: 4469 5119 6025 6172 6950 7140 7932 956
Size: 650 906 147 778 190 792 2182
Fragments arranged by size:
2182 1680 906 879 792 778 650 288
198 190 156 155 147 124 33
BsmBI CGTCTCn'nnnn_
Cuts at: 187 187
Size: 9158
Bsp1286I G_dGCh'C
Cuts at: 461 602 956 1835 1868 1992 2097 2147 2926
Size: 141 354 879 33 124 105 50 779
Cuts at: 2926 3377 3599 3627 3827 3888 4115 4313 4387
Size: 451 222 28 200 61 227 198 74
Cuts at: 4387 4469 4945 5089 5119 5294 5427 5584 6025
Size: 82 476 144 30 175 133 157 441
Cuts at: 6025 6172 6783 6876 6950 7140 7202 7932 461
Size: 147 611 93 74 190 62 730 1687
Fragments arranged by size:
1687 879 779 730 611 476 451 441
354 227 222 200 198 190 175 157
147 144 141 133 124 105 93 82
74 74 62 61 50 33 30 28
BspEI T'CCGG_A
Cuts at: 1019 8378 1019
Size: 7359 1799
BspHI T'CATG_A
Cuts at: 3776 4601 8334 3776
Size: 825 3733 4600
Fragments arranged by size:
4600 3733 825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 1600 1991 2792 3245 3989 4244 4999 5834
Size: 1268 391 801 453 744 255 755 835
Cuts at: 5834 6208 6422 6724 7105 332
Size: 374 214 302 381 2385
Fragments arranged by size:
2385 1268 835 801 755 744 453 391
381 374 302 255 214
BsrBI CCG'CTC
Cuts at: 50 2456 3705 4095 4122 7450 7504 8988 9153
Size: 2406 1249 390 27 3328 54 1484 165
Cuts at: 9153 50
Size: 55
Fragments arranged by size:
3328 2406 1484 1249 390 165 55 54
27
BsrDI GCAATG_nn'
Cuts at: 731 1938 1976 5238 5939 7070 731
Size: 1207 38 3262 701 1131 2819
Fragments arranged by size:
3262 2819 1207 1131 701 38
BsrFI r'CCGG_y
Cuts at: 2154 2214 2815 3957 6122 7156 7337 7546 2154
Size: 60 601 1142 2165 1034 181 209 3766
Fragments arranged by size:
3766 2165 1142 1034 601 209 181 60
BsrGI T'GTAC_A
Cuts at: 5564 5564
Size: 9158
BssSI C'ACGA_G
Cuts at: 7429 7787 7429
Size: 358 8800
BstAPI GCAn_nnn'nTGC
Cuts at: 522 3594 4047 5979 6535 522
Size: 3072 453 1932 556 3145
Fragments arranged by size:
3145 3072 1932 556 453
BstBI TT'CG_AA
Cuts at: 1211 7519 1211
Size: 6308 2850
BstEII G'GTnAC_C
Cuts at: 244 244
Size: 9158
BstXI CCAn_nnnn'nTGG
Cuts at: 975 1373 2070 5199 5388 975
Size: 398 697 3129 189 4745
Fragments arranged by size:
4745 3129 697 398 189
BstYI r'GATC_y
Cuts at: 1 402 1368 1614 2075 2906 4882 5273 5588
Size: 401 966 246 461 831 1976 391 315
Cuts at: 5588 5840 6183 6650 6673 7008 7254 8266 8352
Size: 252 343 467 23 335 246 1012 86
Cuts at: 8352 8364 8710 1
Size: 12 346 449
Fragments arranged by size:
1976 1012 966 831 467 461 449 401
391 346 343 335 315 252 246 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2854 2968 5455 5531 2854
Size: 114 2487 76 6481
Fragments arranged by size:
6481 2487 114 76
BtgI C'CryG_G
Cuts at: 107 188 306 461 479 2183 2303 2357 2847
Size: 81 118 155 18 1704 120 54 490
Cuts at: 2847 3117 3494 4754 5075 5192 5309 5798 5996
Size: 270 377 1260 321 117 117 489 198
Cuts at: 5996 107
Size: 3269
Fragments arranged by size:
3269 1704 1260 490 489 377 321 270
198 155 120 118 117 117 81 54
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5133 5529 7108 7289 7452 5133
Size: 396 1579 181 163 6839
Fragments arranged by size:
6839 1579 396 181 163
BtsI GCAGTG_nn'
Cuts at: 3870 6401 3870
Size: 2531 6627
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1567 1602 2339 2374 1567
Size: 35 737 35 8351
Fragments arranged by size:
8351 737 35 35
DraI TTT'AAA
Cuts at: 6157 6157
Size: 9158
DraIII CAC_nnn'GTG
Cuts at: 2138 6047 2138
Size: 3909 5249
DrdI GACnn_nn'nnGTC
Cuts at: 6864 7722 6864
Size: 858 8300
EaeI y'GGCC_r
Cuts at: 707 2216 2225 2306 2936 3032 3695 3791 3908
Size: 1509 9 81 630 96 663 96 117
Cuts at: 3908 5000 5513 6056 6743 6917 7308 7335 8680
Size: 1092 513 543 687 174 391 27 1345
Cuts at: 8680 707
Size: 1185
Fragments arranged by size:
1509 1345 1185 1092 687 663 630 543
513 391 174 117 96 96 81 27
9
EagI C'GGCC_G
Cuts at: 6743 6743
Size: 9158
EarI CTCTTCn'nnn_
Cuts at: 1813 3005 3805 7181 7391 1813
Size: 1192 800 3376 210 3580
Fragments arranged by size:
3580 3376 1192 800 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3241 4537 7366 7676 7822 8968 3241
Size: 1296 2829 310 146 1146 3431
Fragments arranged by size:
3431 2829 1296 1146 310 146
EcoICRI GAG'CTC
Cuts at: 954 1833 1866 2145 4113 6170 954
Size: 879 33 279 1968 2057 3942
Fragments arranged by size:
3942 2057 1968 879 279 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 427 1326 1375 1483 1605 1837 1934 2092
Size: 78 899 49 108 122 232 97 158
Cuts at: 2092 2131 2299 2454 2752 3826 3841 4129 4288
Size: 39 168 155 298 1074 15 288 159
Cuts at: 4288 4501 4855 4961 5290 5308 5799 5926 6084
Size: 213 354 106 329 18 491 127 158
Cuts at: 6084 6148 6983 7415 8162 8430 349
Size: 64 835 432 747 268 1077
Fragments arranged by size:
1077 1074 899 835 747 491 432 354
329 298 288 268 232 213 168 159
158 158 155 127 122 108 106 97
78 64 49 39 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 328 2659 3853 4822 5772 5908 328
Size: 2331 1194 969 950 136 3578
Fragments arranged by size:
3578 2331 1194 969 950 136
EcoO109I rG'GnC_Cy
Cuts at: 321 541 758 1005 1960 2041 2093 2094 2259
Size: 220 217 247 955 81 52 1 165
Cuts at: 2259 2346 2781 4401 4671 4815 5442 5669 5868
Size: 87 435 1620 270 144 627 227 199
Cuts at: 5868 6297 321
Size: 429 3182
Fragments arranged by size:
3182 1620 955 627 435 429 270 247
227 220 217 199 165 144 87 81
52 1
EcoRI G'AATT_C
Cuts at: 5022 6162 5022
Size: 1140 8018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1769 3186 7177 1769
Size: 1417 3991 3750
Fragments arranged by size:
3991 3750 1417
FspI TGC'GCA
Cuts at: 6939 6939
Size: 9158
HaeII r_GCGC'y
Cuts at: 317 1701 2290 2688 3921 4072 4370 4494 4600
Size: 1384 589 398 1233 151 298 124 106
Cuts at: 4600 5924 6080 6592 6840 7862 8841 317
Size: 1324 156 512 248 1022 979 634
Fragments arranged by size:
1384 1324 1233 1022 979 634 589 512
398 298 248 156 151 124 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2773 2805 3700 3732 4175 4207 4255 4287 4401
Size: 32 895 32 443 32 48 32 114
Cuts at: 4401 4433 4435 4465 4467 4497 5089 5121 5248
Size: 32 2 30 2 30 592 32 127
Cuts at: 5248 5280 5608 5640 5876 5908 7000 7030 7032
Size: 32 328 32 236 32 1092 30 2
Cuts at: 7032 7062 2773
Size: 30 4869
Fragments arranged by size:
4869 1092 895 592 443 328 236 127
114 48 32 32 32 32 32 32
32 32 32 30 30 30 30 2
2 2
HincII GTy'rAC
Cuts at: 2235 5205 6197 2235
Size: 2970 992 5196
Fragments arranged by size:
5196 2970 992
HindIII A'AGCT_T
Cuts at: 1400 1400
Size: 9158
KasI G'GCGC_C
Cuts at: 313 4366 5920 6076 6588 6836 313
Size: 4053 1554 156 512 248 2635
Fragments arranged by size:
4053 2635 1554 512 248 156
KpnI G_GTAC'C
Cuts at: 3894 6178 3894
Size: 2284 6874
MluI A'CGCG_T
Cuts at: 8591 8591
Size: 9158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1061 3443 4253 5795 7829 8013 1061
Size: 2382 810 1542 2034 184 2206
Fragments arranged by size:
2382 2206 2034 1542 810 184
MscI TGG'CCA
Cuts at: 2227 2308 2938 3034 6919 2227
Size: 81 630 96 3885 4466
Fragments arranged by size:
4466 3885 630 96 81
MslI CAynn'nnrTG
Cuts at: 2510 4285 4690 4756 4798 4825 5197 6016 7147
Size: 1775 405 66 42 27 372 819 1131
Cuts at: 7147 7274 2510
Size: 127 4394
Fragments arranged by size:
4394 1775 1131 819 405 372 127 66
42 27
MspA1I CmG'CkG
Cuts at: 169 308 360 558 1560 1887 1905 1995 2559
Size: 139 52 198 1002 327 18 90 564
Cuts at: 2559 2730 2739 3750 4641 4981 5102 5210 5418
Size: 171 9 1011 891 340 121 108 208
Cuts at: 5418 5928 6269 6583 6943 7956 8201 169
Size: 510 341 314 360 1013 245 1126
Fragments arranged by size:
1126 1013 1011 1002 891 564 510 360
341 340 327 314 245 208 198 171
139 121 108 90 52 18 9
NaeI GCC'GGC
Cuts at: 2156 6124 7339 2156
Size: 3968 1215 3975
Fragments arranged by size:
3975 3968 1215
NarI GG'CG_CC
Cuts at: 314 4367 5921 6077 6589 6837 314
Size: 4053 1554 156 512 248 2635
Fragments arranged by size:
4053 2635 1554 512 248 156
NcoI C'CATG_G
Cuts at: 107 188 3494 5192 5309 5798 107
Size: 81 3306 1698 117 489 3467
Fragments arranged by size:
3467 3306 1698 489 117 81
NgoMIV G'CCGG_C
Cuts at: 2154 6122 7337 2154
Size: 3968 1215 3975
Fragments arranged by size:
3975 3968 1215
NspI r_CATG'y
Cuts at: 120 141 4038 4191 4941 5466 5748 6211 6711
Size: 21 3897 153 750 525 282 463 500
Cuts at: 6711 7618 120
Size: 907 1660
Fragments arranged by size:
3897 1660 907 750 525 500 463 282
153 21
PasI CC'CwG_GG
Cuts at: 905 1001 1373 1506 4179 4737 4846 905
Size: 96 372 133 2673 558 109 5217
Fragments arranged by size:
5217 2673 558 372 133 109 96
PciI A'CATG_T
Cuts at: 5462 7614 5462
Size: 2152 7006
PflMI CCAn_nnn'nTGG
Cuts at: 1232 2138 2619 3030 3362 3906 1232
Size: 906 481 411 332 544 6484
Fragments arranged by size:
6484 906 544 481 411 332
PfoI T'CCnGG_A
Cuts at: 858 1143 1702 1859 2069 2834 3803 4783 858
Size: 285 559 157 210 765 969 980 5233
Fragments arranged by size:
5233 980 969 765 559 285 210 157
PmeI GTTT'AAAC
Cuts at: 6157 6157
Size: 9158
PmlI CAC'GTG
Cuts at: 2576 2576
Size: 9158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8297 8329 8297
Size: 32 9126
PpuMI rG'GwC_Cy
Cuts at: 321 541 2041 2781 4671 5442 5669 5868 321
Size: 220 1500 740 1890 771 227 199 3611
Fragments arranged by size:
3611 1890 1500 771 740 227 220 199
PspOMI G'GGCC_C
Cuts at: 2093 3623 3884 2093
Size: 1530 261 7367
Fragments arranged by size:
7367 1530 261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3010 3042 3010
Size: 32 9126
PstI C_TGCA'G
Cuts at: 255 550 1597 1944 2660 2789 2903 3242 3755
Size: 295 1047 347 716 129 114 339 513
Cuts at: 3755 4052 4595 5281 5831 6205 6890 255
Size: 297 543 686 550 374 685 2523
Fragments arranged by size:
2523 1047 716 686 685 550 543 513
374 347 339 297 295 129 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9158
PvuII CAG'CTG
Cuts at: 169 360 1560 1887 1905 1995 2559 2730 2739
Size: 191 1200 327 18 90 564 171 9
Cuts at: 2739 4981 5102 5418 6583 6943 169
Size: 2242 121 316 1165 360 2384
Fragments arranged by size:
2384 2242 1200 1165 564 360 327 316
191 171 121 90 18 9
RsrII CG'GwC_CG
Cuts at: 7353 7353
Size: 9158
SacI G_AGCT'C
Cuts at: 956 1835 1868 2147 4115 6172 956
Size: 879 33 279 1968 2057 3942
Fragments arranged by size:
3942 2057 1968 879 279 33
SacII CC_GC'GG
Cuts at: 309 309
Size: 9158
SalI G'TCGA_C
Cuts at: 6195 6195
Size: 9158
SanDI GG'GwC_CC
Cuts at: 2041 5442 5868 2041
Size: 3401 426 5331
Fragments arranged by size:
5331 3401 426
SapI GCTCTTCn'nnn_
Cuts at: 1813 3005 3805 7181 7391 1813
Size: 1192 800 3376 210 3580
Fragments arranged by size:
3580 3376 1192 800 210
SbfI CC_TGCA'GG
Cuts at: 1597 2660 5831 6205 1597
Size: 1063 3171 374 4550
Fragments arranged by size:
4550 3171 1063 374
ScaI AGT'ACT
Cuts at: 823 3019 3667 8675 823
Size: 2196 648 5008 1306
Fragments arranged by size:
5008 2196 1306 648
SexAI A'CCwGG_T
Cuts at: 537 5030 537
Size: 4493 4665
SfcI C'TryA_G
Cuts at: 33 251 546 1252 1593 1940 2656 2785 2899
Size: 218 295 706 341 347 716 129 114
Cuts at: 2899 3238 3324 3751 4048 4591 5277 5827 6201
Size: 339 86 427 297 543 686 550 374
Cuts at: 6201 6886 7879 8070 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 716 706 686 685 550 543
427 374 347 341 339 297 295 218
191 129 114 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 2224 2224
Size: 9158
SfoI GGC'GCC
Cuts at: 315 4368 5922 6078 6590 6838 315
Size: 4053 1554 156 512 248 2635
Fragments arranged by size:
4053 2635 1554 512 248 156
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9158
SmaI CCC'GGG
Cuts at: 319 6180 319
Size: 5861 3297
SmlI C'TyrA_G
Cuts at: 526 1539 1577 2163 4930 5676 6313 7228 7720
Size: 1013 38 586 2767 746 637 915 492
Cuts at: 7720 7982 526
Size: 262 1702
Fragments arranged by size:
2767 1702 1013 915 746 637 586 492
262 38
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9158
SphI G_CATG'C
Cuts at: 4038 6211 6711 4038
Size: 2173 500 6485
Fragments arranged by size:
6485 2173 500
SspI AAT'ATT
Cuts at: 740 2916 740
Size: 2176 6982
StuI AGG'CCT
Cuts at: 337 4416 4966 337
Size: 4079 550 4529
Fragments arranged by size:
4529 4079 550
StyI C'CwwG_G
Cuts at: 107 188 581 2171 2342 3494 4371 4538 4967
Size: 81 393 1590 171 1152 877 167 429
Cuts at: 4967 5192 5309 5387 5480 5798 6292 6646 107
Size: 225 117 78 93 318 494 354 2619
Fragments arranged by size:
2619 1590 1152 877 494 429 393 354
318 225 171 167 117 93 81 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2918 3130 3796 4210 6741 6874 7333 7542 2918
Size: 212 666 414 2531 133 459 209 4534
Fragments arranged by size:
4534 2531 666 459 414 212 209 133
TatI w'GTAC_w
Cuts at: 140 821 3017 3665 4748 5564 8673 140
Size: 681 2196 648 1083 816 3109 625
Fragments arranged by size:
3109 2196 1083 816 681 648 625
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1782 1814 3505 3537 8675 8707 1782
Size: 32 1691 32 5138 32 2233
Fragments arranged by size:
5138 2233 1691 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1792 3514 3685 6955 1792
Size: 1722 171 3270 3995
Fragments arranged by size:
3995 3270 1722 171
XbaI T'CTAG_A
Cuts at: 6189 6189
Size: 9158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1141 1413 2107 2155 2406 3801 3862 6653 1141
Size: 272 694 48 251 1395 61 2791 3646
Fragments arranged by size:
3646 2791 1395 694 272 251 61 48
XmaI C'CCGG_G
Cuts at: 317 6178 317
Size: 5861 3297
XmnI GAAnn'nnTTC
Cuts at: 673 4006 673
Size: 3333 5825
ZraI GAC'GTC
Cuts at: 4302 4302
Size: 9158
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI BaeI BfrBI BmtI BsiWI BsmI BssHII
BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
SgrAI SnaBI SrfI SwaI XhoI