Restriction map for
pF1KE3824
(Circular) (Six-base) MAPSORT of: pF1KE3824.seq Check: 6918 from: 1 to: 10775
pF1KE3824 10775 bp
With 182 enzymes: SgfI *
November 2, 2012 16:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5587 5587
Size: 10775
AccI GT'mk_AC
Cuts at: 5333 7813 5333
Size: 2480 8295
Acc65I G'GTAC_C
Cuts at: 7791 7791
Size: 10775
AclI AA'CG_TT
Cuts at: 851 10615 851
Size: 9764 1011
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 2266 2596 3341 3697 4316 5027 5032 6233
Size: 1529 330 745 356 619 711 5 1201
Cuts at: 6233 7607 8600 9032 9779 10047 737
Size: 1374 993 432 747 268 1465
Fragments arranged by size:
1529 1465 1374 1201 993 747 745 711
619 432 356 330 268 5
AfeI AGC'GCT
Cuts at: 5455 7231 5455
Size: 1776 8999
AflII C'TTAA_G
Cuts at: 6555 6555
Size: 10775
AflIII A'CryG_T
Cuts at: 676 6160 8127 9231 10208 676
Size: 5484 1967 1104 977 1243
Fragments arranged by size:
5484 1967 1243 1104 977
AgeI A'CCGG_T
Cuts at: 9163 9163
Size: 10775
AhdI GACnn_n'nnGTC
Cuts at: 2521 2521
Size: 10775
AlwNI CAG_nnn'CTG
Cuts at: 6696 8080 9647 6696
Size: 1384 1567 7824
Fragments arranged by size:
7824 1567 1384
ApaLI G'TGCA_C
Cuts at: 3416 4125 9545 3416
Size: 709 5420 4646
Fragments arranged by size:
5420 4646 709
ApoI r'AATT_y
Cuts at: 231 497 536 655 787 1053 1139 1832 1863
Size: 266 39 119 132 266 86 693 31
Cuts at: 1863 3905 4444 4812 5170 5557 5803 7169 7190
Size: 2042 539 368 358 387 246 1366 21
Cuts at: 7190 7779 10128 10384 231
Size: 589 2349 256 622
Fragments arranged by size:
2349 2042 1366 693 622 589 539 387
368 358 266 266 256 246 132 119
86 39 31 21
AseI AT'TA_AT
Cuts at: 20 833 2252 4280 4492 4601 20
Size: 813 1419 2028 212 109 6194
Fragments arranged by size:
6194 2028 1419 813 212 109
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10775
AvaI C'yCGr_G
Cuts at: 5564 6190 7795 5564
Size: 626 1605 8544
Fragments arranged by size:
8544 1605 626
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5289 5322 5289
Size: 33 10742
BamHI G'GATC_C
Cuts at: 7800 7800
Size: 10775
BanI G'GyrC_C
Cuts at: 3544 5927 7791 8205 8453 8488 3544
Size: 2383 1864 414 248 35 5831
Fragments arranged by size:
5831 2383 1864 414 248 35
BanII G_rGCy'C
Cuts at: 2909 7789 8819 2909
Size: 4880 1030 4865
Fragments arranged by size:
4880 4865 1030
BbeI G_GCGC'C
Cuts at: 5931 8209 8457 5931
Size: 2278 248 8249
Fragments arranged by size:
8249 2278 248
BbsI GAAGACnn'nnnn_
Cuts at: 1309 2552 3922 3953 6691 6839 6986 7398 1309
Size: 1243 1370 31 2738 148 147 412 4686
Fragments arranged by size:
4686 2738 1370 1243 412 148 147 31
BciVI GTATCCnnnnn_n'
Cuts at: 1268 7755 8673 9434 10775 1268
Size: 6487 918 761 1341 1268
Fragments arranged by size:
6487 1341 1268 918 761
BclI T'GATC_A
Cuts at: 341 1503 3684 5265 5898 6233 6567 8295 341
Size: 1162 2181 1581 633 335 334 1728 2821
Fragments arranged by size:
2821 2181 1728 1581 1162 633 335 334
BfrBI ATG'CAT
Cuts at: 5410 6105 6325 5410
Size: 695 220 9860
Fragments arranged by size:
9860 695 220
BglII A'GATC_T
Cuts at: 2478 5273 7335 8290 10327 2478
Size: 2795 2062 955 2037 2926
Fragments arranged by size:
2926 2795 2062 2037 955
BlpI GC'TnA_GC
Cuts at: 2157 3773 7887 2157
Size: 1616 4114 5045
Fragments arranged by size:
5045 4114 1616
Bme1580I G_kGCm'C
Cuts at: 1701 3420 3711 4129 5099 6430 8400 8493 9549
Size: 1719 291 418 970 1331 1970 93 1056
Cuts at: 9549 1701
Size: 2927
Fragments arranged by size:
2927 1970 1719 1331 1056 970 418 291
93
BmgBI CAC'GTC
Cuts at: 679 679
Size: 10775
BmrI ACTGGGnnnn_n'
Cuts at: 5526 7435 7987 8068 8403 10547 5526
Size: 1909 552 81 335 2144 5754
Fragments arranged by size:
5754 2144 1909 552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1892 9142 1892
Size: 7250 3525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 3149 6442 7544 725
Size: 2424 3293 1102 3956
Fragments arranged by size:
3956 3293 2424 1102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 684 1591 6582 6618 7951 8830 9322 9620 684
Size: 907 4991 36 1333 879 492 298 1839
Fragments arranged by size:
4991 1839 1333 907 879 492 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 1997 2828 7439 10479 1997
Size: 831 4611 3040 2293
Fragments arranged by size:
4611 3040 2293 831
BsaAI yAC'GTr
Cuts at: 8128 8128
Size: 10775
BsaBI GATnn'nnATC
Cuts at: 6 6012 8289 10326 6
Size: 6006 2277 2037 455
Fragments arranged by size:
6006 2277 2037 455
BsaHI Gr'CG_yC
Cuts at: 5928 6624 8206 8454 9156 10652 5928
Size: 696 1582 248 702 1496 6051
Fragments arranged by size:
6051 1582 1496 702 696 248
BsaWI w'CCGG_w
Cuts at: 132 4575 7964 8485 9163 9437 9584 9995 132
Size: 4443 3389 521 678 274 147 411 912
Fragments arranged by size:
4443 3389 912 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1228 1258 1445 1475 1711 1741 2806 2836 3048
Size: 30 187 30 236 30 1065 30 212
Cuts at: 3048 3078 3215 3245 1228
Size: 30 137 30 8758
Fragments arranged by size:
8758 1065 236 212 187 137 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1249 2120 2473 6176 6857 7549 7552 1249
Size: 871 353 3703 681 692 3 4472
Fragments arranged by size:
4472 3703 871 692 681 353 3
BseYI C'CCAG_C
Cuts at: 2037 7028 7668 8200 8958 9535 10031 2037
Size: 4991 640 532 758 577 496 2781
Fragments arranged by size:
4991 2781 758 640 577 532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 6455 6455
Size: 10775
BsiEI CG_ry'CG
Cuts at: 104 137 8317 8363 9571 104
Size: 33 8180 46 1208 1308
Fragments arranged by size:
8180 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 2130 2909 3420 4129 4198 5714 7716 7789 8567
Size: 779 511 709 69 1516 2002 73 778
Cuts at: 8567 8757 9549 2130
Size: 190 792 3356
Fragments arranged by size:
3356 2002 1516 792 779 778 709 511
190 73 69
BsmI GAATG_Cn'
Cuts at: 1170 2172 2746 3525 3719 5952 6119 6845 1170
Size: 1002 574 779 194 2233 167 726 5100
Fragments arranged by size:
5100 2233 1002 779 726 574 194 167
BsmBI CGTCTCn'nnnn_
Cuts at: 4372 4372
Size: 10775
Bsp1286I G_dGCh'C
Cuts at: 1701 2130 2909 3420 3711 4129 4198 5099 5714
Size: 429 779 511 291 418 69 901 615
Cuts at: 5714 6430 7716 7789 8400 8493 8567 8757 8819
Size: 716 1286 73 611 93 74 190 62
Cuts at: 8819 9549 1701
Size: 730 2927
Fragments arranged by size:
2927 1286 901 779 730 716 615 611
511 429 418 291 190 93 74 73
69 62
BspEI T'CCGG_A
Cuts at: 9995 9995
Size: 10775
BspHI T'CATG_A
Cuts at: 2712 9951 2712
Size: 7239 3536
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 5587 6720 7068 7825 8039 8341 8722 309
Size: 5278 1133 348 757 214 302 381 2362
Fragments arranged by size:
5278 2362 1133 757 381 348 302 214
BsrBI CCG'CTC
Cuts at: 50 9067 9121 10605 10770 50
Size: 9017 54 1484 165 55
Fragments arranged by size:
9017 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 648 895 3936 4941 6831 7335 7458 8687 648
Size: 247 3041 1005 1890 504 123 1229 2736
Fragments arranged by size:
3041 2736 1890 1229 1005 504 247 123
BsrFI r'CCGG_y
Cuts at: 4148 4792 8773 8954 9163 4148
Size: 644 3981 181 209 5760
Fragments arranged by size:
5760 3981 644 209 181
BsrGI T'GTAC_A
Cuts at: 1452 1452
Size: 10775
BssSI C'ACGA_G
Cuts at: 122 9046 9404 122
Size: 8924 358 1493
Fragments arranged by size:
8924 1493 358
BstAPI GCAn_nnn'nTGC
Cuts at: 8152 8152
Size: 10775
BstBI TT'CG_AA
Cuts at: 2652 9136 2652
Size: 6484 4291
BstXI CCAn_nnnn'nTGG
Cuts at: 764 2220 5770 5869 7615 7648 764
Size: 1456 3550 99 1746 33 3891
Fragments arranged by size:
3891 3550 1746 1456 99 33
BstYI r'GATC_y
Cuts at: 1 744 774 1395 1751 2241 2478 2931 3061
Size: 743 30 621 356 490 237 453 130
Cuts at: 3061 5273 7119 7335 7618 7800 8267 8290 8625
Size: 2212 1846 216 283 182 467 23 335
Cuts at: 8625 8871 9883 9969 9981 10327 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2212 1846 1012 743 621 490 467 453
449 356 346 335 283 246 237 216
182 130 86 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 1232 2132 1232
Size: 900 9875
BtgI C'CryG_G
Cuts at: 632 2751 3075 6297 632
Size: 2119 324 3222 5110
Fragments arranged by size:
5110 3222 2119 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 194 8725 8906 9069 194
Size: 8531 181 163 1900
Fragments arranged by size:
8531 1900 181 163
BtsI GCAGTG_nn'
Cuts at: 521 3142 3698 7367 7740 8018 521
Size: 2621 556 3669 373 278 3278
Fragments arranged by size:
3669 3278 2621 556 373 278
DraI TTT'AAA
Cuts at: 800 5210 7774 800
Size: 4410 2564 3801
Fragments arranged by size:
4410 3801 2564
DraIII CAC_nnn'GTG
Cuts at: 1626 2597 7492 1626
Size: 971 4895 4909
Fragments arranged by size:
4909 4895 971
DrdI GACnn_nn'nnGTC
Cuts at: 6536 8481 9339 6536
Size: 1945 858 7972
Fragments arranged by size:
7972 1945 858
EaeI y'GGCC_r
Cuts at: 323 587 7704 8360 8534 8925 8952 10297 323
Size: 264 7117 656 174 391 27 1345 801
Fragments arranged by size:
7117 1345 801 656 391 264 174 27
EagI C'GGCC_G
Cuts at: 8360 8360
Size: 10775
EarI CTCTTCn'nnn_
Cuts at: 914 1235 3048 4370 6837 6875 8798 9008 914
Size: 321 1813 1322 2467 38 1923 210 2681
Fragments arranged by size:
2681 2467 1923 1813 1322 321 210 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8983 9293 9439 10585 8983
Size: 310 146 1146 9173
Fragments arranged by size:
9173 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2907 7787 2907
Size: 4880 5895
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 737 2266 2596 3149 3341 3697 4316 5027
Size: 12 1529 330 553 192 356 619 711
Cuts at: 5027 5032 6233 6442 7544 7607 8600 9032 9779
Size: 5 1201 209 1102 63 993 432 747
Cuts at: 9779 10047 725
Size: 268 1453
Fragments arranged by size:
1529 1453 1201 1102 993 747 711 619
553 432 356 330 268 209 192 63
12 5
EcoO109I rG'GnC_Cy
Cuts at: 429 6751 7914 429
Size: 6322 1163 3290
Fragments arranged by size:
6322 3290 1163
EcoRI G'AATT_C
Cuts at: 4812 7169 7190 7779 4812
Size: 2357 21 589 7808
Fragments arranged by size:
7808 2357 589 21
EcoRV GAT'ATC
Cuts at: 1193 1677 3782 4966 1193
Size: 484 2105 1184 7002
Fragments arranged by size:
7002 2105 1184 484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2280 8794 2095
Size: 185 6514 4076
Fragments arranged by size:
6514 4076 185
FspI TGC'GCA
Cuts at: 8556 8556
Size: 10775
HaeII r_GCGC'y
Cuts at: 1858 5457 5931 7233 8209 8457 9479 10458 1858
Size: 3599 474 1302 976 248 1022 979 2175
Fragments arranged by size:
3599 2175 1302 1022 979 976 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 736 768 1569 1601 2507 2539 2806
Size: 32 544 32 801 32 906 32 267
Cuts at: 2806 2838 2849 2881 3421 3453 4888 4920 8617
Size: 32 11 32 540 32 1435 32 3697
Cuts at: 8617 8647 8649 8679 160
Size: 30 2 30 2256
Fragments arranged by size:
3697 2256 1435 906 801 544 540 267
32 32 32 32 32 32 32 32
30 30 11 2
HincII GTy'rAC
Cuts at: 2303 7814 2303
Size: 5511 5264
HindIII A'AGCT_T
Cuts at: 3574 3623 5204 5213 6558 7514 3574
Size: 49 1581 9 1345 956 6835
Fragments arranged by size:
6835 1581 1345 956 49 9
HpaI GTT'AAC
Cuts at: 2303 2303
Size: 10775
KasI G'GCGC_C
Cuts at: 5927 8205 8453 5927
Size: 2278 248 8249
Fragments arranged by size:
8249 2278 248
KpnI G_GTAC'C
Cuts at: 7795 7795
Size: 10775
MfeI C'AATT_G
Cuts at: 2840 7428 2840
Size: 4588 6187
MluI A'CGCG_T
Cuts at: 10208 10208
Size: 10775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 117 216 311 1513 4030 4731 9446 9630 117
Size: 99 95 1202 2517 701 4715 184 1262
Fragments arranged by size:
4715 2517 1262 1202 701 184 99 95
MscI TGG'CCA
Cuts at: 325 589 7706 8536 325
Size: 264 7117 830 2564
Fragments arranged by size:
7117 2564 830 264
MslI CAynn'nnrTG
Cuts at: 681 1220 1658 1934 2831 3089 3738 3855 7263
Size: 539 438 276 897 258 649 117 3408
Cuts at: 7263 7646 8764 8891 681
Size: 383 1118 127 2565
Fragments arranged by size:
3408 2565 1118 897 649 539 438 383
276 258 127 117
MspA1I CmG'CkG
Cuts at: 1255 2115 2753 4561 6810 6818 7886 8200 8560
Size: 860 638 1808 2249 8 1068 314 360
Cuts at: 8560 9573 9818 1255
Size: 1013 245 2212
Fragments arranged by size:
2249 2212 1808 1068 1013 860 638 360
314 245 8
NaeI GCC'GGC
Cuts at: 8956 8956
Size: 10775
NarI GG'CG_CC
Cuts at: 5928 8206 8454 5928
Size: 2278 248 8249
Fragments arranged by size:
8249 2278 248
NcoI C'CATG_G
Cuts at: 632 3075 632
Size: 2443 8332
NgoMIV G'CCGG_C
Cuts at: 8954 8954
Size: 10775
NsiI A_TGCA'T
Cuts at: 5412 6107 6327 5412
Size: 695 220 9860
Fragments arranged by size:
9860 695 220
NspI r_CATG'y
Cuts at: 455 3747 6164 7828 8328 9235 455
Size: 3292 2417 1664 500 907 1995
Fragments arranged by size:
3292 2417 1995 1664 907 500
PasI CC'CwG_GG
Cuts at: 4946 4946
Size: 10775
PciI A'CATG_T
Cuts at: 6160 9231 6160
Size: 3071 7704
PflMI CCAn_nnn'nTGG
Cuts at: 4750 6314 7402 4750
Size: 1564 1088 8123
Fragments arranged by size:
8123 1564 1088
PfoI T'CCnGG_A
Cuts at: 1071 3066 6525 1071
Size: 1995 3459 5321
Fragments arranged by size:
5321 3459 1995
PmeI GTTT'AAAC
Cuts at: 7774 7774
Size: 10775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9914 9946 9914
Size: 32 10743
PpuMI rG'GwC_Cy
Cuts at: 6751 6751
Size: 10775
PstI C_TGCA'G
Cuts at: 2940 6988 7325 7822 8507 2940
Size: 4048 337 497 685 5208
Fragments arranged by size:
5208 4048 685 497 337
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10775
PvuII CAG'CTG
Cuts at: 1255 2115 4561 6810 8200 8560 1255
Size: 860 2446 2249 1390 360 3470
Fragments arranged by size:
3470 2446 2249 1390 860 360
RsrII CG'GwC_CG
Cuts at: 8970 8970
Size: 10775
SacI G_AGCT'C
Cuts at: 2909 7789 2909
Size: 4880 5895
SacII CC_GC'GG
Cuts at: 2754 2754
Size: 10775
SalI G'TCGA_C
Cuts at: 7812 7812
Size: 10775
SapI GCTCTTCn'nnn_
Cuts at: 3048 8798 9008 3048
Size: 5750 210 4815
Fragments arranged by size:
5750 4815 210
SbfI CC_TGCA'GG
Cuts at: 7325 7822 7325
Size: 497 10278
ScaI AGT'ACT
Cuts at: 928 3233 6587 10292 928
Size: 2305 3354 3705 1411
Fragments arranged by size:
3705 3354 2305 1411
SfcI C'TryA_G
Cuts at: 33 1670 2474 2936 4531 6269 6984 7321 7818
Size: 1637 804 462 1595 1738 715 337 497
Cuts at: 7818 8503 9496 9687 33
Size: 685 993 191 1121
Fragments arranged by size:
1738 1637 1595 1121 993 804 715 685
497 462 337 191
SfoI GGC'GCC
Cuts at: 5929 8207 8455 5929
Size: 2278 248 8249
Fragments arranged by size:
8249 2278 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10775
SmaI CCC'GGG
Cuts at: 6192 7797 6192
Size: 1605 9170
SmlI C'TyrA_G
Cuts at: 699 1570 5564 6555 6561 6597 7930 8845 9337
Size: 871 3994 991 6 36 1333 915 492
Cuts at: 9337 9599 699
Size: 262 1875
Fragments arranged by size:
3994 1875 1333 991 915 871 492 262
36 6
SpeI A'CTAG_T
Cuts at: 67 2367 67
Size: 2300 8475
SphI G_CATG'C
Cuts at: 3747 7828 8328 3747
Size: 4081 500 6194
Fragments arranged by size:
6194 4081 500
SspI AAT'ATT
Cuts at: 1106 4278 1106
Size: 3172 7603
StuI AGG'CCT
Cuts at: 351 351
Size: 10775
StyI C'CwwG_G
Cuts at: 632 2505 3075 4398 5280 7760 7909 8263 632
Size: 1873 570 1323 882 2480 149 354 3144
Fragments arranged by size:
3144 2480 1873 1323 882 570 354 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 4779 5695 7217 8358 8491 8950 9159 348
Size: 4431 916 1522 1141 133 459 209 1964
Fragments arranged by size:
4431 1964 1522 1141 916 459 209 133
TatI w'GTAC_w
Cuts at: 926 1452 3231 3483 5021 5879 6119 6585 7415
Size: 526 1779 252 1538 858 240 466 830
Cuts at: 7415 10290 926
Size: 2875 1411
Fragments arranged by size:
2875 1779 1538 1411 858 830 526 466
252 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1228 1260 1736 1768 3048 3080 6797 6829 10292
Size: 32 476 32 1280 32 3717 32 3463
Cuts at: 10292 10324 1228
Size: 32 1679
Fragments arranged by size:
3717 3463 1679 1280 476 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 8572 8572
Size: 10775
XbaI T'CTAG_A
Cuts at: 7806 7806
Size: 10775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 166 667 3108 3304 4137 4773 5770 5869 6672
Size: 501 2441 196 833 636 997 99 803
Cuts at: 6672 8270 166
Size: 1598 2671
Fragments arranged by size:
2671 2441 1598 997 833 803 636 501
196 99
XhoI C'TCGA_G
Cuts at: 5564 5564
Size: 10775
XmaI C'CCGG_G
Cuts at: 6190 7795 6190
Size: 1605 9170
XmnI GAAnn'nnTTC
Cuts at: 950 1781 2648 4301 5116 6416 950
Size: 831 867 1653 815 1300 5309
Fragments arranged by size:
5309 1653 1300 867 831 815
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AleI AlfI AloI ApaI AscI AvrII BbvCI
BcgI BglI BmtI Bpu10I BsiWI BssHII BstEII BstZ17I
ClaI CspCI EcoNI FseI FspAI NdeI NheI NotI
NruI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI